Insights into SusCD-mediated glycan import by a prominent gut symbiont

In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a “pedal bin” transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the β2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism

Model-Based Verification, Optimization, Synthesis and Performance Evaluation of Real-Time Systems

International audienceThis article aims at providing a concise and precise Travellers Guide, Phrase Book or Reference Manual to the timed automata modeling formalism introduced by Alur and Dill [8, 9]. The paper gives comprehensive definitions of timed automata, priced (or weighted) timed automata, and timed games and highlights a number of results on associated decision problems related to model checking, equivalence checking, optimal scheduling, the existence of winning strategies, and then statistical model checking

Comparison of CdS films deposited from chemical baths containing different doping impurities. Thin Solid Films 2005

Abstract The results obtained for CdS films chemically deposited from solutions containing different impurities known as donor type dopants for CdS (chlorine, iodine, boron and indium) are reported. CdS films were deposited onto glass and CuInSe 2 absorber substrates at 85 8C. The deposition baths were ammonia solutions containing 0.001 M Cd 2+ , 0.02 M ammonium compound and 0.002 M thiourea and CdS was deposited at pH 10.3. After deposition, the layers were heated at 200 8C for 30 min in vacuum. The results confirm that the electrical resistivity of CdS films doped with boron and chlorine was dependent on doping level. At the same time, characteristics of CuInSe 2 solar cells with differently doped CdS did not show any remarkable dependence on doping level and nature. The buried homojunction model is proposed to explain obtained results.

Cooperative Learning in Information Security Education: Teaching Secret Sharing Concepts

Information security is an important yet challenging area of education. Much of the difficulty lies in the complexity of security schemes and the often perceived disjoint between theory and practice. This paper investigates the topic of adopting cooperative learning for teaching secret sharing concepts and schemes. The aim of this is to motivate and cultivate an interest in students to learn more about the topic. By designing hands-on cooperative learning activities, the goal is help students appreciate the connection between theory and practice, and to be able to help and encourage each other in their understanding of the area. In this paper, we analyze the suitability of this group-based learning approach in relation to addressing the five essential elements of cooperative learning

Tartu Ülikooli Õppe- ja Katsemetskonna Kastre Peravalla metsandike plaanid (kvartalid nr 200-293)

PlaanidTartu Ülikooli Õppe- ja Katsemetskonna Kastre Peravalla metsandike takseerkirjeldused aastatest 1922, 1923. Kvartalite nr 200-293 käsikirjalised originaalplaanid on mõõtkavas 1:2500. Koloreeritud plaanid sisaldavad kvartalite numbreid, leppemärkide seletusi, ilmakaari, graafilist mõõtkava, autorite nimesid. Autorite nimedes võib esineda eksimusi. Loetamatud nimed puuduvad autorite loendis

Tartu Ülikooli Õppe- ja Katsemetskonna Kastre Peravalla metsandike plaanid (kvartalid nr 101-199)

PlaanidTartu Ülikooli Õppe- ja Katsemetskonna Kastre Peravalla metsandike takseerkirjeldused aastatest 1922, 1923, 1924. Kvartalite nr 101-199 käsikirjalised originaalplaanid on mõõtkavas 1:2500. Koloreeritud plaanid sisaldavad kvartalite numbreid, leppemärkide seletusi, ilmakaari, graafilist mõõtkava, autorite nimesid. Autorite nimedes võib esineda eksimusi. Loetamatud nimed puuduvad autorite loendis