Hormonal responses during two different concurrent-training trials in youth elite soccer players: does changing the organisation of training impact the hormonal response to concurrent exercise?

BACKGROUND: There are no data describing the acute hormonal responses to concurrent-training programmes in youth elite soccer players. Therefore, the aim of this study was to describe the total testosterone (T), cortisol (C), and growth hormone (hGH) responses during two same-day concurrent-training (CT) trials in elite soccer players. METHODS: n=13 youth elite players (age: 17.0±0.2 yrs; height, 1.80±0.07 m; body mass, 73.1±5.7 kg; O2 max, 64.4±4.8ml-1.kg-1.min-1) from an English premier league soccer club completed two CT trials. ‘Trial 1’ (CT1); E (10.30h) followed by S (14.00h) and Trial 2 (CT2); strength-training (S) 09.00h followed by a soccer-specific endurance-training session (E) at 10.30h. Venous blood samples were collected at 5 time-points around training and food intake (T1; 08.00h, T2; 09.45h, T3; 12.30h, T4; 13.45h and T5; 15.15h) and analysed for T (nmol/L) and C (nmol/L) and hGH (ug/L). RESULTS: There was no main effects found between exercise conditions for any hormones (T; P=0.22, C; P=0.07, hGH; P=0.21). Effect size analysis revealed a moderate effect for T at T3 (ES=0.63, CT1; 18.4±3.8, CT2; 15.7±4.7 nmol/L-1). A moderate effect for T area under the curve (AUC) was observed between conditions (CT1; 300±76 versus CT2; 244 ± 81 [AU]; ES=0.71). A moderate effect was apparent for C concentrations T4 in (ES=-0.95, CT1; 230±69, CT2; 314±105 nmol/L-1). Moderate effect sizes were observed at T3 and T4 (ES=0.82, CT1; 1.28±1.17, CT2; 0.47±0.75, ES=0.72, CT1; 0.11±0.05, CT2; 0.07±0.06 ug/L-1 respectively). A moderate effect for hGH AUC was observed between trials (CT1; 14±11 versus CT2; 5±9; [AU], ES=-1.08). CONCLUSIONS: The organisation of the concurrent-training protocols used in this study has a negligible impact upon the acute T, C and hGH in youth elite soccer players

A narrative review on female physique athletes: the physiological and psychological implications of weight management practices

Physique competitions are events in which aesthetic appearance and posing ability are valued above physical performance. Female physique athletes are required to possess high lean body mass and extremely low fat mass in competition. As such, extended periods of reduced energy intake and intensive training regimens are used with acute weight loss practices at the end of the precompetition phase. This represents an increased risk for chronic low energy availability and associated symptoms of relative energy deficiency in sport, compromising both psychological and physiological health. Available literature suggests that a large proportion of female physique athletes report menstrual irregularities (e.g., amenorrhea and oligomenorrhea), which are unlikely to normalize immediately postcompetition. Furthermore, the tendency to reduce intakes of numerous essential micronutrients is prominent among those using restrictive eating patterns. Following competition, reduced resting metabolic rate, and hyperphagia, is also a concern for these female athletes, which can result in frequent weight cycling, distorted body image, and disordered eating/eating disorders. Overall, female physique athletes are an understudied population, and the need for more robust studies to detect low energy availability and associated health effects is warranted. This narrative review aims to define the natural female physique athlete, explore some of the physiological and psychological implications of weight management practices experienced by female physique athletes, and propose future research directions

Maturity-associated considerations for training load, injury risk, and physical performance within youth soccer: One size does not fit all

Biological maturation can be defined as the timing and tempo of progress to achieve a mature state. The estimation of age of peak height velocity (PHV) or percentage of final estimated adult stature attainment (%EASA) is typically used to inform the training process in young athletes. In youth soccer, maturity-related changes in anthropometric and physical fitness characteristics are diverse among individuals, particularly around PHV. During this time, players are also at an increased risk of sustaining an overuse or growth-related injury. As a result, the implementation of training interventions can be challenging. The purpose of this review is to (1) highlight and discuss many of the methods that can be used to estimate maturation in the applied setting and (2) discuss the implications of manipulating training load around PHV on physical development and injury risk. We also have provided key stakeholders with a practical online tool for estimating player maturation status (see online supplementary maturity estimation tool(s)). Whilst estimating maturity using predictive equations is useful in guiding the training process, practitioners should be aware of its limitations. To increase the accuracy and usefulness of data, it is also vital that sports scientists implement reliable testing protocols at predetermined time-points

Caring through Science and Autonomy: Terry’s Story

While it is accepted that sport coaches should safeguard participants, this book argues that coaches have a duty of care that moves beyond protection and involves the development of caring relationships with athletes

Reliability of ‘in-season’ fitness assessments in youth elite soccer players: a working model for practitioners and coaches.

Purpose: To assess the reliability of a battery of field tests when performed around habitual training during one micro-cycle of the ‘in-season’ period in youth elite soccer players. Methods: n=19 English Premier League academy players (mean ± SD: age, 18.3±0.2 years; stature, 1.80±0.05 m; body mass, 76.5±7.5 kg; V̇O2 max; 62.3±4.38 ml·kg-1·min-1 Sum of 8 skinfolds; 64.8±17.4 mm) performed; ‘1RM half-back squat (HBS)’, ‘vertical jump’ (VJ), ‘Yo-Yo IR2’, ’5, 10 & 20 m, sprint’, ‘the agility T-test’ and a ‘repeated sprint ability’ assessments around their habitual ‘in-season’ training and match-play on two occasions. Typical error (TE), coefficient of variation (CV), the smallest worthwhile change (SWC) was then calculated between trials. Test usefulness was then calculated by comparing the ‘SWC’ to the TE and rated as; good, OK or marginal. The smallest difference needed to be considered real (MD), was also calculated using the equation (TE×1.96×√2). Results: Most assessments demonstrated relatively high levels of reproducibility (CV; 0.3 - 4.3 %) to witness an “almost certain beneficial change” (i.e.,>MD) changes of approximately 5% (RSAbest, RSAmean, 10m & 20m sprint, ‘agility T test’ and 1RM HBS), and 11.5% (VJ, 5m sprint, and Yo-Yo IR2) are needed. Conclusions: The present training and testing ‘model’ is reliable and could be used when evaluating the fitness of highly trained youth elite soccer players during the ‘in-season’ period

How the love of muscle can break a heart: Impact of anabolic androgenic steroids on skeletal muscle hypertrophy, metabolic and cardiovascular health.

It is estimated 6.4% of males and 1.6% of females globally use anabolic-androgenic steroids (AAS), mostly for appearance and performance enhancing reasons. In combination with resistance exercise, AAS use increases muscle protein synthesis resulting in skeletal muscle hypertrophy and increased performance. Primarily through binding to the androgen receptor, AAS exert their hypertrophic effects via genomic, non-genomic and anti-catabolic mechanisms. However, chronic AAS use also has a detrimental effect on metabolism ultimately increasing the risk of cardiovascular disease (CVD). Much research has focused on AAS effects on blood lipids and lipoproteins, with abnormal concentrations of these associated with insulin resistance, hypertension and increased visceral adipose tissue (VAT). This clustering of interconnected abnormalities is often referred as metabolic syndrome (MetS). Therefore, the aim of this review is to explore the impact of AAS use on mechanisms of muscle hypertrophy and markers of MetS. AAS use markedly decreases high-density lipoprotein cholesterol (HDL-C) and increases low-density lipoprotein cholesterol (LDL-C). Chronic AAS use also appears to cause higher fasting insulin levels and impaired glucose tolerance and possibly higher levels of VAT; however, research is currently lacking on the effects of AAS use on glucose metabolism. While cessation of AAS use can restore normal lipid levels, it may lead to withdrawal symptoms such as depression and hypogonadism that can increase CVD risk. Research is currently lacking on effective treatments for withdrawal symptoms and further long-term research is warranted on the effects of AAS use on metabolic health in males and females

Effect of menstrual cycle phase, menstrual irregularities and hormonal contraceptive use on anterior knee laxity and non-contact anterior cruciate ligament injury occurrence in women: a protocol for a systematic review and meta-analysis.

Exercising women report three to six times more ACL tears than men, which happen, in the majority of cases, with a non-contact mechanism. This sex disparity has, in part, been attributed to the differences in reproductive hormone profiles between men and women. Many studies have shown that anterior knee (AK) laxity and the rate of non-contact ACL injuries vary across the menstrual cycle, but these data are inconsistent. Similarly, several studies have investigated the potential protective effect of hormonal contraceptives on non-contact ACL injuries, but their conclusions are also variable. The purpose of this systematic review and meta-analysis is to, identify, evaluate and summarise the effects of endogenous and exogenous ovarian hormones on AK laxity (primary outcome) and the occurrence of non-contact ACL injuries (secondary outcome) in women. We will perform a systematic search for all observational studies conducted on this topic. Studies will be retrieved by searching electronic databases, clinical trial registers, author's personal files and cross-referencing selected studies. Risk of bias will be assessed using the Newcastle Ottawa Quality Assessment Scale for Cohort and Case-Control Studies. Certainty in the cumulative evidence will be assessed using the Grading of Recommendations Assessment, Development and Evaluation approach. The meta-analyses will use a Bayesian approach to address specific research questions in a more intuitive and probabilistic manner. This review is registered on the international database of prospectively registered systematic reviews (PROSPERO; CRD42021252365)

Methods to predict the timing and status of biological maturation in male adolescent soccer players: A narrative systematic review

The aim of this review was to summarise the methods used to predict and assess maturity status and timing in adolescent, male, academy soccer players. A systematic search was conducted on PubMed, Scopus, Web of Science, CINAHL, Medline and SPORTDiscus. Only experimental studies including male, academy players aged U9-U18 years registered with a professional soccer club were included. The methodological quality of the included studies was assessed using guidelines from the Framework of Potential Biases. Fifteen studies fulfilled our inclusion criteria. Studies were mainly conducted in European countries (n = 12). In total, 4,707 players were recruited across all 15 studies, with an age range of 8-18 years. Five studies were longitudinal, two studies were mixed-method designs and eight studies were cross-sectional. Due to high heterogeneity within the studies, a meta-analysis was not performed. Our findings provided no equivalent estimations of adult height, skeletal age, or age at PHV. Discrepancies were evident between actual and predicted adult height and age at PHV. The Bayley-Pinneau (1952), Tanner-Whitehouse 2 (1983) and Khamis-Roche (1994) methods produced estimates of adult height within 1cm of actual adult height. For age at PHV, both Moore (2015) equations produced the closest estimates to actual age at PHV, and the Fransen (2018) equation correlated highly with actual age at PHV (>90%), even when the period between chronological age and age at PHV was large. Medical imaging techniques (e.g., Magnetic Resonance Imaging, X-Ray, Dual energy X-ray Absorptiometry) demonstrated high intra/inter-rater reliability (ICC = 0.83-0.98) for skeletal maturity assessments. The poor concordance between invasive and non-invasive methods, is a warning to practitioners to not use these methods interchangeably for assessing maturational status and timing in academy soccer players. Further research with improved study designs is required to validate these results and improve our understanding of these methods when applied in this target population

The Effect of Carbohydrate Restriction on Lipids, Lipoproteins, and Nuclear Magnetic Resonance-Based Metabolites: CALIBER, a Randomised Parallel Trial

Low-carbohydrate high-fat (LCHF) diets can be just as effective as high-carbohydrate, lower-fat (HCLF) diets for improving cardiovascular disease risk markers. Few studies have compared the effects of the UK HCLF dietary guidelines with an LCHF diet on lipids and lipoprotein metabolism using high-throughput NMR spectroscopy. This study aimed to explore the effect of an ad libitum 8-week LCHF diet compared to an HCLF diet on lipids and lipoprotein metabolism and CVD risk factors. For 8 weeks, n = 16 adults were randomly assigned to follow either an LCHF (n = 8, <50 g CHO p/day) or an HCLF diet (n = 8). Fasted blood samples at weeks 0, 4, and 8 were collected and analysed for lipids, lipoprotein subclasses, and energy-related metabolism markers via NMR spectroscopy. The LCHF diet increased (p < 0.05) very small VLDL, IDL, and large HDL cholesterol levels, whereas the HCLF diet increased (p < 0.05) IDL and large LDL cholesterol levels. Following the LCHF diet alone, triglycerides in VLDL and HDL lipoproteins significantly (p < 0.05) decreased, and HDL phospholipids significantly (p < 0.05) increased. Furthermore, the LCHF diet significantly (p < 0.05) increased the large and small HDL particle concentrations compared to the HCLF diet. In conclusion, the LCHF diet may reduce CVD risk factors by reducing triglyceride-rich lipoproteins and improving HDL functionality

Translational Regulation of Utrophin by miRNAs

Background Utrophin is the autosomal homolog of dystrophin, the product of the Duchenne Muscular Dystrophy (DMD) locus. Its regulation is of therapeutic interest as its overexpression can compensate for dystrophin's absence in animal models of DMD. The tissue distribution and transcriptional regulation of utrophin have been characterized extensively, and more recently translational control mechanisms that may underlie its complex expression patterns have begun to be identified. Methodology/Principal Findings Using a variety of bioinformatic, molecular and cell biology techniques, we show that the muscle isoform utrophin-A is predominantly suppressed at the translational level in C2C12 myoblasts. The extent of translational inhibition is estimated to be ~99% in C2C12 cells and is mediated by both the 5′- and 3′-UTRs of the utrophin-A mRNA. In this study we identify five miRNAs (let-7c, miR-150, miR-196b, miR-296-5p, miR-133b) that mediate the repression, and confirm repression by the previously identified miR-206. We demonstrate that this translational repression can be overcome by blocking the actions of miRNAs, resulting in an increased level of utrophin protein in C2C12 cells. Conclusions/Significance The present study has identified key inhibitory mechanisms featuring miRNAs that regulate utrophin expression, and demonstrated that these mechanisms can be targeted to increase endogenous utrophin expression in cultured muscle cells. We suggest that miRNA-mediated inhibitory mechanisms could be targeted by methods similar to those described here as a novel strategy to increase utrophin expression as a therapy for DMD