707 research outputs found
Plasma Sialic Acid Levels in Normal Beagles
Glycoproteins are found in cell membraines, plasma and in various secretions. Many plasma proteins have carbohydrates covalently bound to them. Carbohydrate unites will containe hexoses, N-acetylglucoseamine, Nacetylgalactoseamine, fucose and sialic acid (N-acetylneuraminic acid)
Impact of carprofen administration on stress and nociception responses of calves to cautery dehorning
Citation: Stock, M. L., Barth, L. A., Van Engen, N. K., Millman, S. T., Gehring, R., Wang, C., . . . Coetzee, J. F. (2016). Impact of carprofen administration on stress and nociception responses of calves to cautery dehorning. Journal of Animal Science, 94(2), 542-555. doi:10.2527/jas2015-9510The objective of this study was to investigate the effects of carprofen administered immediately before cautery dehorning on nociception and stress. Forty Holstein calves aged approximately 6 to 8 wk old were either placebo treated and sham dehorned (n = 10) or cautery dehorned following administration of carprofen (1.4 mg/kg) subcutaneously (n = 10) or orally (n = 10) or a subcutaneous and oral placebo (n = 10) in a randomized, controlled trial. All animals were given a cornual nerve block using lidocaine before dehorning. Response variables including mechanical nociception threshold, ocular temperature, heart rate, and respiratory rate were measured before and following cautery dehorning for 96 h. Blood samples were also collected over 96 h following dehorning and analyzed for plasma cortisol and substance P concentrations by RIA. Plasma carprofen concentration and ex vivo PGE2 concentrations were also determined for this time period. Average daily gain was calculated for 7 d after dehorning. Data were analyzed using a linear mixed effects model with repeated measures, controlling for baseline values by their inclusion as a covariate in addition to planned contrasts. Dehorning was associated with decreased nociception thresholds throughout the study and a stress response immediately after dehorning, following the loss of local anesthesia, and 48 h after dehorning compared with sham-dehorned calves. Carprofen was well absorbed after administration and reached concentrations that inhibited ex vivo PGE2 concentrations for 72 h (subcutaneous) and 96 h (oral) compared with placebo-treated calves (P < 0.05). Carprofen-treated calves tended to be less sensitive (P = 0.097) to nociceptive threshold tests. Overall, at the dosing regimen studied, the effect of carprofen on sensitivity and stress following cautery dehorning was minimal. Consideration of route of administration and dose determination studies may be warranted
Modelling radiation-induced cell cycle delays
Ionizing radiation is known to delay the cell cycle progression. In
particular after particle exposure significant delays have been observed and it
has been shown that the extent of delay affects the expression of damage such
as chromosome aberrations. Thus, to predict how cells respond to ionizing
radiation and to derive reliable estimates of radiation risks, information
about radiation-induced cell cycle perturbations is required. In the present
study we describe and apply a method for retrieval of information about the
time-course of all cell cycle phases from experimental data on the mitotic
index only. We study the progression of mammalian cells through the cell cycle
after exposure. The analysis reveals a prolonged block of damaged cells in the
G2 phase. Furthermore, by performing an error analysis on simulated data
valuable information for the design of experimental studies has been obtained.
The analysis showed that the number of cells analyzed in an experimental sample
should be at least 100 to obtain a relative error less than 20%.Comment: 19 pages, 11 figures, accepted for publication in Radiation and
Environmental Biophysic
Recommended from our members
Cross-accent intelligibility of speech in noise: Long-term familiarity and short-term familiarization
Listeners must cope with a great deal of variability in the speech signal, and thus theories of speech perception must also account for variability, which comes from a number of sources, including variation between accents. It is well-known that there is a processing cost when listening to speech in an accent other than one’s own, but recent work has suggested that this cost is reduced when listening to a familiar accent widely represented in the media, and/or when short amounts of exposure to an accent are provided. Little is known, however, about how these factors (long-term familiarity and short-term familiarization with an accent) interact. The current study tested this interaction by playing listeners difficult-to-segment sentences in noise, before and after a familiarization period where the same sentences were heard in the clear, allowing us to manipulate short-term familiarization. Listeners were speakers of either Glasgow English or Standard Southern British English, and they listened to speech in either their own or the other accent, thereby allowing us to manipulate long-term familiarity. Results suggest that both long-term familiarity and short-term familiarization mitigate the perceptual processing costs of listening to an accent that is not one’s own, but seem not to compensate for them entirely, even when the accent is widely heard in the media
Fiscal Multipliers and Public Debt Dynamics in Consolidations
The success of a consolidation in reducing the debt ratio depends crucially on the value of the multiplier, which measures the impact of consolidation on growth, and on the reaction of sovereign yields to such a consolidation.
We present a theoretical framework that formalizes the re spo nse of the public debt ratio to fiscal consolidations in relation to the value of fiscal multipliers, the starting debt level and the cyclical elasticity of the budget balance. We also assess the role of markets confidence to fiscal consolidations under al ternative scenarios. We find that with high levels of public debt and sizeable fiscal multipliers , debt ratios are likely to increase in the short term in response to fiscal consolidations. Hence, the typical horizon for a consolidation during crises episo des to reduce the debt ratio is two - three years , although this horizon depends critically on the size and persistence of fiscal multipliers and the reaction of financial markets.
Anyway, such undesired debt responses are mainly short - lived.
This effect is very unlikely in non - crisis times, as it requires a number of conditions difficult to observe at the same time , especially high fiscal multipliers
Preliminary seismological and geological studies of the San Fernando, California, earthquake of February 9 1971
The San Fernando earthquake was the largest earthquake to occur in the metropolitan Los
Angeles area in more than 50 years. It has tentatively been assigned a magnitude, M_L of 6.6, a focal
depth of 13.0 km, and an epicentral location about 12 km east of Newhall, California, at 34°24.0'N,
118°23.7'W (Figure 1), but these figures undoubtedly will be modified as further data become available.
Although the focal depth is not as well defined as the epicenter, it is consistent with other
observations suggesting thrusting on a fault plane dipping north about 45 ° and breaking the surface
in the Sylmar-San Fernando area (Figure 1). It should be emphasized that the hypocenter of
the main shock represents only the point of initial rupture. Breaking, presumably, then propagated
southward and upward from this point, so that the main geological and engineering effects
were observed farther south where the fault was shallower and the displacement greater. The location
of the main shock is based on readings from permanent stations of the Caltech network, as
well as the U. S. Geological Survey station at Point Mugu (SBLG) and the California Department
of Water Resources stations at Pyramid (PYR) and Cedar Springs (CSP). Portable Caltech seismographs
were installed in the epicentral area as early as 3 hr following the main shock, and,
within a few days, there were at least 30 portable units in the region operated by various groups
and agencies
Robust estimation of microbial diversity in theory and in practice
Quantifying diversity is of central importance for the study of structure,
function and evolution of microbial communities. The estimation of microbial
diversity has received renewed attention with the advent of large-scale
metagenomic studies. Here, we consider what the diversity observed in a sample
tells us about the diversity of the community being sampled. First, we argue
that one cannot reliably estimate the absolute and relative number of microbial
species present in a community without making unsupported assumptions about
species abundance distributions. The reason for this is that sample data do not
contain information about the number of rare species in the tail of species
abundance distributions. We illustrate the difficulty in comparing species
richness estimates by applying Chao's estimator of species richness to a set of
in silico communities: they are ranked incorrectly in the presence of large
numbers of rare species. Next, we extend our analysis to a general family of
diversity metrics ("Hill diversities"), and construct lower and upper estimates
of diversity values consistent with the sample data. The theory generalizes
Chao's estimator, which we retrieve as the lower estimate of species richness.
We show that Shannon and Simpson diversity can be robustly estimated for the in
silico communities. We analyze nine metagenomic data sets from a wide range of
environments, and show that our findings are relevant for empirically-sampled
communities. Hence, we recommend the use of Shannon and Simpson diversity
rather than species richness in efforts to quantify and compare microbial
diversity.Comment: To be published in The ISME Journal. Main text: 16 pages, 5 figures.
Supplement: 16 pages, 4 figure
Архетип свобода у контексті французької політичної теорії та історії
Розглянуто сучасні підходи щодо аналізу політичної ментальності. У межах політологічного аналізу окреслено коло проблем, які потребують вирішення з використанням підходів психології. Зроблено висновок про те, що архетип “свобода” становить важливий елемент політичної ментальності французів.Modern approaches of analysis of political mentality are considered. Within the limits of political science analysis outlined circle of problems which need decision with the use of approaches of psychology. A conclusion is done that archetype freedom makes the important element of political mentality of French’s
Half-metallicity and Slater-Pauling behavior in the ferromagnetic Heusler alloys
Introductory chapter for the book "Halfmetallic Alloys - Fundamentals and
Applications" to be published in the series Springer Lecture Notes on Physics,
P. H. Dederichs and I. Galanakis (eds). It contains a review of the theoretical
work on the half-metallic Heusler alloys.Comment: Introductory chapter for the book "Halfmetallic Alloys - Fundamentals
and Applications" to be published in the series Springer Lecture Notes on
Physics, P. H. Dederichs and I. Galanakis (eds
- …