Latent Dirichlet Allocation (LDA) for improving the topic modeling of the official bulletin of the spanish state (BOE)

Since Internet was born most people can access fully free to a lot sources of information. Every day a lot of web pages are created and new content is uploaded and shared. Never in the history the humans has been more informed but also uninformed due the huge amount of information that can be access. When we are looking for something in any search engine the results are too many for reading and filtering one by one. Recommended Systems (RS) was created to help us to discriminate and filter these information according to ours preferences. This contribution analyses the RS of the official agency of publications in Spain (BOE), which is known as "Mi BOE'. The way this RS works was analysed, and all the meta-data of the published documents were analysed in order to know the coverage of the system. The results of our analysis show that more than 89% of the documents cannot be recommended, because they are not well described at the documentary level, some of their key meta-data are empty. So, this contribution proposes a method to label documents automatically based on Latent Dirichlet Allocation (LDA). The results are that using this approach the system could recommend (at a theoretical point of view) more than twice of documents that it now does, 11% vs 23% after applied this approach

La literatura no sexista en las aulas de Educación Infantil: transmedialidad en “La Bella y la Bestia” y evolución de los arquetipos de género. Propuesta de un canon literario coeducativo

El presente trabajo tiene como objetivo mostrar la relación existente entre la Literatura Infantil y la transmisión de roles y estereotipos sexistas que contribuyen a perpetuar una cultura basada en valores patriarcales y discriminatorios. El propósito de esta investigación es concienciar acerca de la importancia de ofrecer una adecuada educación a niños y niñas, partiendo de un modelo coeducativo que promueva la igualdad de oportunidades, considerando la diversidad como un elemento de riqueza social y cultural. Para ello se ha presentado un marco teórico en el que se explica cómo en la infancia los niños y niñas adquieren los roles de género a través de la transmisión social. Se ha considerado la importancia de la perspectiva de género, valorando las aportaciones de autoras relevantes como Virginia Woolf, Simone de Beauvoir, Judith Butler o Monique Wittig, entre otras. En este marco se ha valorado la importancia de la educación literaria como medio de transmisión de valores, revisando para ello los géneros más adecuados para la etapa de Educación Infantil. Para mostrar cómo la literatura influye en el desarrollo de niños y niñas se ha realizado un análisis desde la crítica feminista de algunos cuentos clásicos aplicando una metodología comparatista para la evolución de los arquetipos de género, considerando principalmente distintas versiones de “La Bella y La Bestia” y su transmedialidad. Finalmente, se propone un modelo coeducativo a través del cual se promueve la igualdad de oportunidades entre hombres y mujeres. La educación literaria es esencial y para ello se presenta un canon literario analizado y comentado de cuarenta y dos cuentos no sexistas, a modo de guías de lectura como herramienta útil en la praxis educativa que se puede incluir en las aulas de Educación Infantil. Este canon se ha estructurado y categorizado para abordar cinco dimensiones primordiales y emergentes en la educación literaria no sexista: remodelación de las princesas, romper roles y estereotipos de género y fomentar la igualdad, perspectiva modernizada de los clásicos, construcción de su identidad, respeto y tolerancia hacia la diversidad afectivo-sexual.Con esta propuesta pretendemos que desde la infancia reciban modelos de conducta diversos que promuevan una actitud crítica y reflexiva para contribuir a la construcción de su personalidad, permitiendo a niños, a niñas y a los adultos tomar parte en la eliminación de la cultura heteropatriarcal. <br /

Inhibition of MMP-9-dependent degradation of gelatin, but not other MMP-9 substrates, by the MMP-9 hemopexin domain blades 1 and 4

11 p.-5 fig.-1 tab.Degradation and remodeling of the extracellular matrix by matrix metalloproteinases (MMPs) plays important roles in normal development, inflammation, and cancer. MMP-9 efficiently degrades the extracellular matrix component gelatin, and the hemopexin domain of MMP-9 (PEX9) inhibits this degradation. To study the molecular basis of this inhibition, we generated GST fusion proteins containing PEX9 or truncated forms corresponding to specific structural blades (B1-B4) of PEX9. GST-PEX9 inhibited MMP-9-driven gelatin proteolysis, measured by gelatin zymography, FITC-gelatin conversion, and DQ-gelatin degradation assays. However, GST-PEX9 did not prevent the degradation of other MMP-9 substrates, such as a fluorogenic peptide, αB crystalline, or nonmuscular actin. Therefore, PEX9 may inhibit gelatin degradation by shielding gelatin and specifically preventing its binding to MMP-9. Accordingly, GST-PEX9 also abolished the degradation of gelatin by MMP-2, confirming that PEX9 is not an MMP-9 antagonist. Moreover, GST-B4 and, to a lesser extent, GST-B1 also inhibited gelatin degradation by MMP-9, indicating that these regions are responsible for the inhibitory activity of PEX9. Accordingly, ELISAs demonstrated that GST-B4 and GST-B1 specifically bound to gelatin. Our results establish new functions of PEX9 attributed to blades B4 and B1 and should help in designing specific inhibitors of gelatin degradation.This work was supported by Grant SAF2012-31613 and Red Temática de Investigación Cooperativa en Cáncer Grant RD12/0036/0061 from the Ministry of Economy and Competitivity (Spain) (to A. G.-P.); by Grant S2010/ BMD-2314 (to A. G.-P.) from the Comunidad de Madrid/European Union;and by the Concerted Research Actions Grant GOA 2013–2015 and the fund for Scientific Research of Flanders (to G. O.).Peer reviewe

El marketing digital como herramienta estratégica de apoyo al crecimiento y desarrollo organizacional de Rocanet

El uso de herramientas digitales en las organizaciones es fundamental hoy en día, debido a la transformación y los avances tecnológicos que ha sufrido el mundo empresarial, que necesita recurrir a la innovación constante para adaptarse a estos cambios. En este sentido, el objetivo de la investigación se centró en analizar la incidencia del marketing digital como herramienta de apoyo estratégico para lograr el crecimiento y desarrollo organizacional de la empresa Rocanet. El estudio utilizó una metodología no experimental con enfoque cuantitativo-cualitativo, de tipo transversal. Para la obtención de los datos se realizó una encuesta a una muestra no probabilística intencional, conformada por colaboradores de la empresa y clientes, así como una entrevista al administrador. Los resultados obtenidos en estos instrumentos sirvieron para saber que la organización no aplica el marketing digital. Se elaboró una matriz que permitió identificar las debilidades, oportunidades, amenazas y fortalezas que presenta la empresa frente a la competencia y realizar una propuesta de acciones estratégicas. Se demostró que la no utilización del marketing digital en esta organización ha provocado la pérdida de clientes, ventas y visibilidad. Se concluye que Rocanet necesita contar con un plan de marketing digital que le permita mejorar su competitividad.PALABRAS CLAVE: marketing digital; estrategias; desarrollo organizacional; Internet.Digital marketing as a strategic support tool for the growth and organizational development of RocanetABSTRACTThe use of digital tools in organizations is essential today, due to the transformation and technological advances that the business world has undergone, which needs to resort to constant innovation to adapt to these changes. In this sense, the objective of the research focused on analyzing the incidence of digital marketing as a strategic support tool to achieve the growth and organizational development of the Rocanet company. The study used a non-experimental methodology with a quantitative-qualitative approach, of a cross-sectional type. To obtain the data, a survey was carried out on an intentional non-probabilistic sample, made up of company collaborators and clients, as well as an-interview with the administrator. The results obtained in these instruments served to know that the organization does not apply digital marketing. A matrix was elaborated that allowed to identify the weaknesses, opportunities, threats and strengths that the company presents in front of the competition and to make a proposal of strategic actions. It was shown that the non-use of digital marketing in this organization has caused the loss of customers, sales and visibility. It is concluded that the Rocanet company needs to have a digital marketing plan that allows it to improve its competitiveness.KEYWORDS: digital marketing; strategies; organizational development; Internet

Boletín oficial del Estado: análisis de metadatos, detección de errores y recomendaciones de mejora

El acceso a la información pública (la que generan las instituciones públicas) es un derecho reconocido tanto por la legislación europea (Unión Europea, 2001) como por la española (España, 1992; 1997; 1999; 2013). Los organismos del estado español están obligados a poner a disposición del ciudadano de manera gratuita y transparente todo lo que generan a menos que existan motivos de seguridad nacional. Muchos organismos estatales, autonómicos y locales han optado por crear sistemas de información o plataformas de información pública en forma de boletines informativos. Este artículo tiene como finalidad conocer cómo se encuentra a nivel documental el principal boletín informativo del estado español, el Boletín oficial del Estado (BOE) estudiando los metadatos de los documentos publicados y analizado en profundidad los que se utilizan para la descripción documental. Los resultados reflejan la ausencia de descriptores documentales en más del 89% del total del corpus documental del BOE. En los últimos años esta cifra apenas supera el 50%. El trabajo finaliza esbozando algunas líneas de mejora

Sphingosine-1-phosphate activates chemokine-promoted myeloma cell adhesion and migration involving α4β1 integrin function

51 p.-7 fig.-1 tab.-2 fig.supl.Myeloma cell adhesion dependent on α4β1 integrin is crucial for the progression of multiple myeloma (MM). The α4β1-dependent myeloma cell adhesion is up-regulated by the chemokine CXCL12, and pharmacological blockade of the CXCL12 receptor CXCR4 leads to defective myeloma cell homing to bone marrow (BM). Sphingosine-1-phosphate (S1P) regulates immune cell trafficking upon binding to G-protein-coupled receptors. Here we show that myeloma cells express S1P1, a receptor for S1P. We found that S1P up-regulated the α4β1-mediated myeloma cell adhesion and transendothelial migration stimulated by CXCL12. S1P promoted generation of high-affinity α4β1 that efficiently bound the α4β1 ligand VCAM-1, a finding that was associated with S1P-triggered increase in talin-β1 integrin association. Furthermore, S1P cooperated with CXCL12 for enhancement of α4β1-dependent adhesion strengthening and spreading. CXCL12 and S1P activated the DOCK2-Rac1 pathway, which was required for stimulation of myeloma cell adhesion involving α4β1. Moreover, in vivo analyses indicated that S1P contributes to optimizing the interactions of MM cells with the BM microvasculture and for their lodging inside the bone marrow. The regulation of α4β1-dependent adhesion and migration of myeloma cells by CXCL12-S1P combined activities might have important consequences for myeloma disease progressionThis study was supported by the Ministerio de Ciencia e Innovación (Grant Nos SAF2011-24022 to JT, SAF2009-07035 to AGP, SAF2009-11037 to AH, RD06/0020/0011 to JT and AGP, RD06/0020/0006 to NCG and MG and PI081825 to MG), the Comunidad de Madrid (Grant No. P2010/BMD-2314 to AGP, JT and AH) and the Fundación de Investigación Médica Mutua Madrileña (to AGP and MG). AH was also funded by the FP7-People-2009-RG (Grant No. 246655), a Ramón y Cajal Fellowship (Grant No. RYC-2007-00697) and the Pro-CNIC Foundation.Peer reviewe

A 17-residue sequence from the matrix metalloproteinase-9 (MMP-9) hemopexin domain binds α4β1 integrin and inhibits MMP-9-induced functions in chronic lymphocytic leukemia B cells

13 páginas, 7 figuras, 2 tablas -- PAGS nros. 27601-27613We previously showed that pro-matrix metalloproteinase-9 (proMMP-9) binds to B chronic lymphocytic leukemia (B-CLL) cells and contributes to B-CLL progression by regulating cell migration and survival. Induction of cell survival involves a non-proteolytic mechanism and the proMMP-9 hemopexin domain (PEX9). To help design specific inhibitors of proMMP-9-cell binding, we have now characterized B-CLL cell interaction with the isolated PEX9. B-CLL cells bound soluble and immobilized GST-PEX9, but not GST, and binding was mediated by α4β1 integrin. The ability to recognize PEX9 was observed in all 20 primary samples studied irrespective of their clinical stage or prognostic marker phenotype. By preparing truncated forms of GST-PEX9 containing structural blades B1B2 or B3B4, we have identified B3B4 as the primary α4β1 integrin-interacting region within PEX9. Overlapping synthetic peptides spanning B3B4 were then tested in functional assays. Peptide P3 (FPGVPLDTHDVFQYREKAYFC), a sequence present in B4 or smaller versions of this sequence (peptides P3a/P3b), inhibited B-CLL cell adhesion to GST-PEX9 or proMMP-9, with IC50 values of 138 and 279 μm, respectively. Mutating the two aspartate residues to alanine rendered the peptides inactive. An anti-P3 antibody also inhibited adhesion to GST-PEX9 and proMMP-9. GST-PEX9, GST-B3B4, and P3/P3a/P3b peptides inhibited B-CLL cell transendothelial migration, whereas the mutated peptide did not. B-CLL cell incubation with GST-PEX9 induced intracellular survival signals, namely Lyn phosphorylation and Mcl-1 up-regulation, and this was also prevented by the P3 peptides. The P3 sequence may, therefore, constitute an excellent target to prevent proMMP-9 contribution to B-CLL pathogenesisThis work was supported by Grants SAF2009–07035 and RTICC RD06/0020/0011 (to A. G.-P.) and RTICC RD06/0020/0080 (to M. J. T.) from the Ministerio de Ciencia e Innovación, Spain, and by a grant from the Fundación Puerta de Hierro (to J. A. G. M.)Peer reviewe

Matrix metalloproteinase-9 induces a pro-angiogenic profile in chronic lymphocytic leukemia cells

27 p.-3 fig.-2 tab.Increased angiogenesis is commonly observed in chronic lymphocytic leukemia (CLL) tissues in correlation with advanced disease. CLL cells express pro- and anti-angiogenic genes and acquire a pro-angiogenic pattern upon interaction with the microenvironment. Because MMP-9 (a microenvironment component) plays important roles in solid tumor angiogenesis, we have studied whether MMP-9 influenced the angiogenic pattern in CLL cells. Immunofluorescence analyses confirmed the presence of MMP-9 in CLL tissues. MMP-9 interaction with CLL cells increased their MMP-9 expression and secretion into the medium. Accordingly, the conditioned media of MMP-9-primed CLL cells significantly enhanced endothelial cell proliferation, compared to control cells. MMP-9 also increased VEGF and decreased TSP-1 and Ang-2 expression, all at the gene and protein level, inducing a pro-angiogenic pattern in CLL cells. Mechanistic analyses demonstrated that downregulation of the selected gene TSP-1 by MMP-9 involved α4β1 integrin, Src kinase family activity and the STAT3 transcription factor. Regulation of angiogenic genes is a novel contribution of MMP-9 to CLL pathology.This work was supported by grants SAF2015-69180R from the Ministerio de Economía y Competitividad (Spain)/Fondo Europeo de Desarrollo Regional (FEDER), European Union; S2010/BMD-2314 from the Comunidad de Madrid/European Union (AGP.); and by the Concerted Research Actions (KU Leuven C1 Grant C16/17/010) and the Research Foundation of Flanders (FWO-Vlaanderen, to EUB, PEVdS, and GO).Peer reviewe

Effects of Aesthetic Chills on a Cardiac Signature of Emotionality

Previous studies have shown that a cardiac signature of emotionality (referred to as EK, which can be computed from the standard 12 lead electrocardiogram, ECG), predicts inter-individual differences in the tendency to experience and express positive emotion. Here, we investigated whether EK values can be transiently modulated during stimulation with participant-selected music pieces and film scenes that elicit strongly positive emotion. The phenomenon of aesthetic chills, as indicated by measurable piloerection on the forearm, was used to accurately locate moments of peak emotional responses during stimulation. From 58 healthy participants, continuous EK values, heart rate, and respiratory frequency were recorded during stimulation with film scenes and music pieces, and were related to the aesthetic chills. EK values, as well as heart rate, increased significantly during moments of peak positive emotion accompanied by piloerection. These results are the first to provide evidence for an influence of momentary psychological state on a cardiac signature of emotional personality (as reflected in EK values). The possibility to modulate ECG amplitude signatures via stimulation with emotionally significant music pieces and film scenes opens up new perspectives for the use of emotional peak experiences in the therapy of disorders characterized by flattened emotionality, such as depression or schizoid personality disorder

UR-1505, ¿un nuevo salicilato para la enfermedad inflamatoria intestinal?

Capítulos 5, 6, 7 y 8 escritos en inglésTesis Univ. Granada. Departamento de Farmacología. Leída el 20 de marzo de 200