Quels sont les bénéfices d'une immunisation passive postexpositionnelle contre la rougeole ? : Revue Cochrane pour le praticien

Question clinique : Une patiente de 43 ans connue pour un lupus érythémateux disséminé et une néphropathie lupique stable et traitée par hydroxychloroquine et mycophénolate mofétil vous rapporte avoir eu contact il y a quatre jours avec un enfant atteint de la rougeole. Elle ne présente aucun symptôme et son dernier bilan vaccinal montre un taux d'anticorps contre la rougeole non protecteur. La patiente devrait-elle recevoir une immunisation active ou passive contre la rougeole? Contexte En Suisse : la couverture vaccinale contre la rougeole n'est pas assez importante pour empêcher sa diffusion. L'immunisation passive avec des immunoglobulines est destinée aux personnes non immunes exposées à la rougeole, recommandée en Suisse aux personnes à haut risque. Cette revue visait à évaluer l'efficacité et l'innocuité de l'injection intramusculaire ou de la perfusion intraveineuse d'immunoglobulines pour prévenir la rougeole chez les personnes susceptibles avant l'apparition des symptômes

Editorial research and the publication process in biomedicine and health: Report from the Esteve Foundation Discussion Group, December 2012.

Despite the fact that there are more than twenty thousand biomedical journals in the world, research into the work of editors and publication process in biomedical and health care journals is rare. In December 2012, the Esteve Foundation, a non-profit scientific institution that fosters progress in pharmacotherapy by means of scientific communication and discussion organized a discussion group of 7 editors and/or experts in peer review biomedical publishing. They presented findings of past editorial research, discussed the lack of competitive funding schemes and specialized journals for dissemination of editorial research, and reported on the great diversity of misconduct and conflict of interest policies, as well as adherence to reporting guidelines. Furthermore, they reported on the reluctance of editors to investigate allegations of misconduct or increase the level of data sharing in health research. In the end, they concluded that if editors are to remain gatekeepers of scientific knowledge they should reaffirm their focus on the integrity of the scientific record and completeness of the data they publish. Additionally, more research should be undertaken to understand why many journals are not adhering to editorial standards, and what obstacles editors face when engaging in editorial research

How do authors of systematic reviews deal with research malpractice and misconduct in original studies? A cross-sectional analysis of systematic reviews and survey of their authors.

OBJECTIVES: To study whether systematic reviewers apply procedures to counter-balance some common forms of research malpractice such as not publishing completed research, duplicate publications, or selective reporting of outcomes, and to see whether they identify and report misconduct. DESIGN: Cross-sectional analysis of systematic reviews and survey of their authors. PARTICIPANTS: 118 systematic reviews published in four journals (Ann Int Med, BMJ, JAMA, Lancet), and the Cochrane Library, in 2013. MAIN OUTCOMES AND MEASURES: Number (%) of reviews that applied procedures to reduce the impact of: (1) publication bias (through searching of unpublished trials), (2) selective outcome reporting (by contacting the authors of the original studies), (3) duplicate publications, (4) sponsors' and (5) authors' conflicts of interest, on the conclusions of the review, and (6) looked for ethical approval of the studies. Number (%) of reviewers who suspected misconduct are reported. The procedures applied were compared across journals. RESULTS: 80 (68%) reviewers confirmed their data. 59 (50%) reviews applied three or more procedures; 11 (9%) applied none. Unpublished trials were searched in 79 (66%) reviews. Authors of original studies were contacted in 73 (62%). Duplicate publications were searched in 81 (69%). 27 reviews (23%) reported sponsors of the included studies; 6 (5%) analysed their impact on the conclusions of the review. Five reviews (4%) looked at conflicts of interest of study authors; none of them analysed their impact. Three reviews (2.5%) looked at ethical approval of the studies. Seven reviews (6%) suspected misconduct; only 2 (2%) reported it explicitly. Procedures applied differed across the journals. CONCLUSIONS: Only half of the systematic reviews applied three or more of the six procedures examined. Sponsors, conflicts of interest of authors and ethical approval remain overlooked. Research misconduct is sometimes identified, but rarely reported. Guidance on when, and how, to report suspected misconduct is needed

How to assess the external validity of therapeutic trials: a conceptual approach

Background External validity of study results is an important issue from a clinical point of view. From a methodological point of view, however, the concept of external validity is more complex than it seems to be at first glance. Methods Methodological review to address the concept of external validity. Results External validity refers to the question whether results are generalizable to persons other than the population in the original study. The only formal way to establish the external validity would be to repeat the study for that specific target population. We propose a three-way approach for assessing the external validity for specified target populations. (i) The study population might not be representative for the eligibility criteria that were intended. It should be addressed whether the study population differs from the intended source population with respect to characteristics that influence outcome. (ii) The target population will, by definition, differ from the study population with respect to geographical, temporal and ethnical conditions. Pondering external validity means asking the question whether these differences may influence study results. (iii) It should be assessed whether the study's conclusions can be generalized to target populations that do not meet all the eligibility criteria. Conclusion Judging the external validity of study results cannot be done by applying given eligibility criteria to a single target population. Rather, it is a complex reflection in which prior knowledge, statistical considerations, biological plausibility and eligibility criteria all have plac

Graphic Classes in the Worldwide Classroom: A Comparison of Two MOOC Experiences

Graphics are present in the day-to-day professional practice of architects and engineers, not only to receive and transmit information, but also to design and create. Students who are accepted on university courses have varied curriculum vitae, and some may initially lack skills. Consequently, engineering schools have developed a Massive Open Online Course (MOOC) entitled “The Language of Engineering” (ELI), which reviews basic geometry concepts and develops spatial intelligence, among others. The Barcelona School of Architecture has produced “From reality to design. From design to augmented reality” (RA), which covers topics including traditional architectural representation and the latest techniques. The goal of this study was to explain and analyse the main characteristics and learning strategies of these two MOOC (strengths, weaknesses and opportunities for improvement). The results show that although strategies vary depending on the subjects, the contents and exercises should be practical and adapted to students (interests, level, time availability and aesthetics), always considering motivation as a key point (gamification). These topics have been found to have a considerable influence on the success of a MOOC. Therefore, the conclusions should be considered in subsequent versions of these courses and other MOOCs.Postprint (author's final draft

Attitudes of editors of core clinical journals about whether systematic reviews are original research: a mixed-methods study.

In 2009, not all journal editors considered systematic reviews (SRs) to be original research studies, and not all PubMed Core Clinical Journals published SRs. The aim of this study was to conduct a new analysis about editors' opinion regarding SRs as original research. We conducted a survey and qualitative interview study of journal editors. All editors listed as editor-in chief of 118 PubMed Core Clinical Journals. We contacted editors via email and asked them whether they considered SRs original research, whether they published SRs in the journal and, if yes, in which section. We searched PubMed for any SRs (or meta-analyses) published in the included journals in 2017; if we did not find any, we hand-searched these journals. Editors were invited to participate in a follow-up qualitative interview study. We received responses from 73 editors representing 72 (62%) journals. Fifty-two (80%) editors considered SRs original research, either for any type of SR (65%) or only for SRs with a meta-analysis (15%) and almost all (91%) of editors published SRs. Compared with the results of the 2009 study of Core Clinical Journals, a similar proportion of editors considered SRs to be original studies (71%), accepted SRs as original on certain condition such as presence of meta-analysis (14%) or published SRs (94%). Interviews with editors showed that they used various criteria to decide whether a SR is original research, including methodology, reproducibility, originality of idea and level of novelty. The majority of editors of core clinical journals consider that SRs are original research. Among editors, there was no uniform approach to defining what makes a SR, or any study, original. This indicates that the concepts of originality of SRs and research are evolving and that this would be a relevant topic for further discussion

Chlamydia pneumoniae aggravates vein graft intimal hyperplasia in a rat model

<p>Abstract</p> <p>Background</p> <p>Along with angioplasty, autologus vein grafts are commonly used for artery bypass grafting in patients with advanced arterial stenosis and drug-resistant angina pectoris. Although initially a successful procedure, long-term functionality is limited due to proliferation and migration of smooth muscle cells. Like in atherosclerosis, common chronic infections caused by viruses and bacteria may contribute to this process of vein graft failure. Here we investigated the possible role of <it>Chlamydia pneumoniae </it>(<it>Cpn</it>) in the pathogenesis of venous graft failure in an experimental animal model. In 2 groups (n = 10 rats/group), an epigastric vein-to-common femoral artery interposition graft was placed. Immediately thereafter, rats were infected with <it>Cpn </it>(5*10⁸IFU) or injected with control solutions. Rats were sacrificed three weeks after surgery and the grafts were harvested for morphometrical and immunohistochemical analysis.</p> <p>Results</p> <p><it>Cpn </it>administration immediately after vein grafting resulted in a significant increase in medial cross-sectional area, wall thickness and total wall area. There were no significant differences in T-cell or macrophage influx. Likewise, although positive immunostaining for both HSP60 and CRP could be detected, no differences were found between groups. Based on the observation that the number of cells/μm²was also not altered, we conclude that Cpn infection stimulates smooth muscle cell proliferation by hereunto unknown molecular mechanisms, resulting in a significant increase in intimal hyperplasia.</p> <p>Conclusion</p> <p>In conclusion, in a well defined animal model we present here for the first time evidence for a role of <it>Chlamydia pneumoniae </it>in the process of venous graft failure.</p

Benzophénanthridines isolées de Zanthoxylum psammophilum

Dix-huit composés dont deux nouvelles benzophénanthridines la 8-méthoxy-7,8-dihydrofagaridine 1 et la 8-acétonyl-7,8-dihydrofagaridine 2 ont été isolés des racines de Zanthoxylum psammophilum (Rutaceae). La structure de ces composés (1-18) a été déterminée principalement par l’utilisation de la spectroscopie de RMN 1D (1H et 13C) et 2D (COSY, NOESY, HSQC, HMBC). Le composé 1 a montré une activité antimicrobienne sur S. Aureus.Mots clés: Rutaceae, alcaloïdes, 8-méthoxy-7,8-dihydrofagaridine, 8- acétonyl-7,8-dihydrofagaridin

Grain morphological characterization and protein content of sixty-eight local rice (Oryza sativa L) cultivars from Cameroon

Rice (Oryza sativa L.) cultivated in Cameroon is appreciated by consumers for its nutritive quality and good taste. Diversity of 68 local rice cultivars was investigated via grain morphology and protein content characterization. The size and shape of grains were determined and used with yield parameters to classify the cultivars and perform Principal Component Analysis (PCA). Total protein content and glutelin content of eight selected cultivars (CMRGNd, CMRGDn, CMRGTï, CMRTBa, CMRDWb, CMRDTc3, CMRDTx5 and CMRDTx6) were evaluated by Bradford assay and correlation analysis of all the parameters studied was performed. Long size grains (42) were predominant over extra-long (16), medium (9) and short (1) grains. Slender shaped grains (36) were distinguished as well as medium (28) and bold (4) grains. The 68 cultivars were grouped into four clusters independent of their origins. PCA revealed three principal components accounting for 74.4% of total variation. Highest total protein content was observed in CMRGNd (14.3%) and highest glutelin content in CMRGDn (10.1 mgEqvBSA/g DW). Pearson correlation of the different variables revealed no significant correlation between total protein and glutelin contents with the agro-morphological parameters evaluated in this study. This suggests that none of these parameters could be descriptor for protein content. Positive correlation between grain length and yield (r = 0.7) suggests grain length as yield descriptor