A survey on wireless ad hoc networks

A wireless ad hoc network is a collection of wireless nodes that can dynamically self-organize into an arbitrary and temporary topology to form a network without necessarily using any pre-existing infrastructure. These characteristics make ad hoc networks well suited for military activities, emergency operations, and disaster recoveries. Nevertheless, as electronic devices are getting smaller, cheaper, and more powerful, the mobile market is rapidly growing and, as a consequence, the need of seamlessly internetworking people and devices becomes mandatory. New wireless technologies enable easy deployment of commercial applications for ad hoc networks. The design of an ad hoc network has to take into account several interesting and difficult problems due to noisy, limited-range, and insecure wireless transmissions added to mobility and energy constraints. This paper presents an overview of issues related to medium access control (MAC), routing, and transport in wireless ad hoc networks and techniques proposed to improve the performance of protocols. Research activities and problems requiring further work are also presented. Finally, the paper presents a project concerning an ad hoc network to easily deploy Internet services on low-income habitations fostering digital inclusion8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

Wireless ad hoc networks on underserved communities: an efficient solution for interactive digital TV

The Brazilian government intends to use the Digital TV technology as a vehicle of digital inclusion on underserved communities. The wireless ad hoc network is a low-cost, scalable and easy solution to implement the return channel. This work analyzes the performance of an ad hoc return channel using the wireless IEEE 802.11 technology in different Brazilian geographical scenarios. The results show that a high connectivity is achieved when more than 20% of the nodes are turned on, regardless of the position of the gateway. The influence of the number of hops and the number of transmitting nodes is also analyzed. A minimum throughput of 2 Mbps can be reached for increasing number of hops in the forwarding chain for a one-node transmission. Besides, when the number of transmitting nodes increases, the aggregated throughput can achieve 3.5 Mbps. The results show that the ad hoc network is a promising solution for the return channel of the interactive Digital TV.8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

A Phylogenomic Approach for the Analysis of Colistin Resistance-Associated Genes in Klebsiella Pneumoniae, its Mutational Diversity and Implications for Phenotypic Resistance

The emergence of carbapenemase-producing Klebsiella pneumoniae strains has triggered the use of old antibiotics such as colistin. This is driving the emergence of colistin resistance in multidrug-resistant strains that underlie life-threatening infections. This study analyses the mutational diversity of 22 genes associated with colistin resistance in 140 K. pneumoniae clinical isolates integrated in a high-resolution phylogenetic scenario. Colistin susceptibility was accessed by broth microdilution. A total of 98 isolates were susceptible and 16 were resistant, 10 of which were carbapenemase producers. Across the 22 genes examined, 171 non-synonymous mutations and 9 mutations associated with promoter regions were found. Eighty-five isolates had a truncation and/or deletion in at least one of the 22 genes. However, only seven mutations, the complete deletion of mgrB or insertion sequence (IS)-mediated disruption, were exclusively observed in resistant isolates. Four of these (mgrBIle13fs, pmrBGly207Asp, phoQHis339Asp and ramAIle28Met) comprised novel mutations that are potentially involved in colistin resistance. One strain bore a ISEcp1-blaCTX-M-15::mgrB disruption, underlying co-resistance to third-generation cephalosporins and colistin. Moreover, the high-resolution phylogenetic context shows that most of the mutational diversity spans multiple phylogenetic clades, and most of the mutations previously associated with colistin resistance are clade-associated and present in susceptible isolates, showing no correlation with colistin resistance. In conclusion, the present study provides relevant data on the genetic background of genes involved with colistin resistance deeply rooted across monophyletic groups and provides a better understanding of the genes and mutations involved in colistin resistance.info:eu-repo/semantics/publishedVersio

A survey on wireless ad hoc networks

A wireless ad hoc network is a collection of wireless nodes that can dynamically self-organize into an arbitrary and temporary topology to form a network without necessarily using any pre-existing infrastructure. These characteristics make ad hoc networks well suited for military activities, emergency operations, and disaster recoveries. Nevertheless, as electronic devices are getting smaller, cheaper, and more powerful, the mobile market is rapidly growing and, as a consequence, the need of seamlessly internetworking people and devices becomes mandatory. New wireless technologies enable easy deployment of commercial applications for ad hoc networks. The design of an ad hoc network has to take into account several interesting and difficult problems due to noisy, limited-range, and insecure wireless transmissions added to mobility and energy constraints. This paper presents an overview of issues related to medium access control (MAC), routing, and transport in wireless ad hoc networks and techniques proposed to improve the performance of protocols. Research activities and problems requiring further work are also presented. Finally, the paper presents a project concerning an ad hoc network to easily deploy Internet services on low-income habitations fostering digital inclusion8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

Protection against symptomatic dengue infection by neutralizing antibodies varies by infection history and infecting serotype

Dengue viruses (DENV1–4) are the most prevalent arboviruses in humans and a major public health concern. Understanding immune mechanisms that modulate DENV infection outcome is critical for vaccine development. Neutralizing antibodies (nAbs) are an essential component of the protective immune response, yet their measurement often relies on a single cellular substrate and partially mature virions, which does not capture the full breadth of neutralizing activity and may lead to biased estimations of nAb potency. Here, we analyze 125 samples collected after one or more DENV infections but prior to subsequent symptomatic or inapparent DENV1, DENV2, or DENV3 infections from a long-standing pediatric cohort study in Nicaragua. By assessing nAb responses using Vero cells with or without DC-SIGN and with mature or partially mature virions, we find that nAb potency and the protective NT50 cutoff are greatly influenced by cell substrate and virion maturation state. Additionally, the correlation between nAb titer and protection from disease depends on prior infection history and infecting serotype. Finally, we uncover variations in nAb composition that contribute to protection from symptomatic infection differently after primary and secondary prior infection. These findings have important implications for identifying antibody correlates of protection for vaccines and natural infections

Early Start Peritoneal Dialysis: Technique Survival in Long-Term Follow-Up

Background/Aims: Peritoneal dialysis (PD) has gained interest over the last decade as a viable option for early start dialysis. It is still unknown if shorter break-in periods and less time for proper patient evaluation and training could influence technique survival in comparison to planned-start PD. Methods: A prospective and observational study that compared technique survival in a cohort of patients who started either early or planned PD. Early start PD was defined as break-in period from 3 to 14 days with no previous nephrologist follow-up or patient training. Results: A total of 154 patients were included (40 as early start PD), followed by a median time of 381 days. Comparing early vs. planned-start PD, groups were similar concerning age 56 (40; 70) vs. 48 (32; 63) years, p=0.071, body mass index (BMI) 23.3 ± 4.2 vs. 23.8 ± 4.0 kg/m2, p=0.567 and male gender (60 vs. 48%, p=0.201), respectively. Comparing early vs. planned-start groups, there were no differences regarding PD dropout for peritonitis (7.5 vs. 11.4%, p=0.764), catheter dysfunction (12.5 vs. 17.5%, p=0.619) and patient burnout (0 vs. 4.4%, p=0.328), respectively. Less patients in early start group quit PD for peritoneal membrane failure in comparison to planned-start group (2.5 vs. 16.7%, p=0.026). In multivariate cox-regression analysis, the only factors independently associated with technique failure were BMI> 25 kg/m² (p=0.033) and Diabetes Mellitus (p=0.013), whereas no differences regarding early vs. planned-PD start were observed (p=0.184). Conclusion: Despite the adverse scenario for initiating dialysis, early start PD had similar outcomes in comparison to planned-start PD in long-term follow-up

Wireless ad hoc networks on underserved communities: an efficient solution for interactive digital TV

The Brazilian government intends to use the Digital TV technology as a vehicle of digital inclusion on underserved communities. The wireless ad hoc network is a low-cost, scalable and easy solution to implement the return channel. This work analyzes the performance of an ad hoc return channel using the wireless IEEE 802.11 technology in different Brazilian geographical scenarios. The results show that a high connectivity is achieved when more than 20% of the nodes are turned on, regardless of the position of the gateway. The influence of the number of hops and the number of transmitting nodes is also analyzed. A minimum throughput of 2 Mbps can be reached for increasing number of hops in the forwarding chain for a one-node transmission. Besides, when the number of transmitting nodes increases, the aggregated throughput can achieve 3.5 Mbps. The results show that the ad hoc network is a promising solution for the return channel of the interactive Digital TV.8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

In defence of the entity of Macaronesia as a biogeographical region

Since its coinage ca. 1850 AD by Philip Barker Webb, the biogeographical region of Macaronesia, consisting of the North Atlantic volcanic archipelagos of the Azores, Madeira with the tiny Selvagens, the Canaries and Cabo Verde, and for some authors different continental coastal strips, has been under dispute. Herein, after a brief introduction on the terminology and purpose of regionalism, we recover the origins of the Macaronesia name, concept and geographical adscription, as well as its biogeographical implications and how different authors have positioned themselves, using distinct terrestrial or marine floristic and/or faunistic taxa distributions and relationships for accepting or rejecting the existence of this biogeographical region. Four main issues related to Macaronesia are thoroughly discussed: (i) its independence from the Mediterranean phytogeographical region; (ii) discrepancies according to different taxa analysed; (iii) its geographical limits and the role of the continental enclave(s), and, (iv) the validity of the phytogeographical region level. We conclude that Macaronesia has its own identity and a sound phytogeographical foundation, and that this is mainly based on three different floristic components that are shared by the Macaronesian core (Madeira and the Canaries) and the outermost archipelagos (Azores and Cabo Verde). These floristic components are: (i) the Palaeotropical‐Tethyan Geoflora, formerly much more widely distributed in Europe and North Africa and currently restricted to the three northern archipelagos (the Azores, Madeira and the Canaries); (ii) the African Rand Flora, still extant in the coastal margins of Africa and Arabia, and present in the southern archipelagos (Madeira, the Canaries and Cabo Verde), and (iii) the Macaronesian neoendemic floristic component, represented in all the archipelagos, a result of allopatric diversification promoted by isolation of Mediterranean ancestors that manage to colonize Central Macaronesia and, from there, the outer archipelagos. Finally, a differentiating floristic component recently colonized the different archipelagos from the nearest continental coast, providing them with different biogeographic flavours

Theoretical and experimental study of the excitonic binding energy\ud in GaAs/AlGaAs single and coupled double quantum wells

This paper discusses the theoretical and experimental results obtained for the excitonic binding energy (Eb) in a set of single and coupled double quantum wells (SQWs and CDQWs) of GaAs/AlGaAs with different Al concentrations (Al%) and inter-well barrier thicknesses. To obtain the theoretical Eb the method proposed by Mathieu, Lefebvre and Christol (MLC) was used, which is based on the idea of fractional-dimension space, together with the approach proposed by Zhao et al., which extends the MLC method for application in CDQWs. Through magnetophotoluminescence (MPL) measurements performed at 4 K with magnetic fields ranging from 0 T to 12 T, the diamagnetic shift curves were plotted and adjusted using two expressions: one appropriate to fit the curve in the range of low intensity fields and another for the range of high intensity fields, providing the experimental Eb values. The effects of increasing the Al% and the inter-well barrier thickness on Eb are discussed. The Eb reduction when going from the SQW to the CDQW with 5 Å inter-well barrier is clearly observed experimentally for 35% Al concentration and this trend can be noticed even for concentrations as low as 25% and 15%, although the Eb variations in these latter cases are within the error bars. As the Zhao's approach is unable to describe this effect, the wave functions and the probability densities for electrons and holes were calculated, allowing us to explain this effect as being due to a decrease in the spatial superposition of the wave functions caused by the thin inter-well barrier.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (Fundação Araucária)Fundo de Apoio ao Ensino, à Pesquisa e à Extensão (FAPE-UEL)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Instituto Nacional de Ciência e Tecnologia de Nanodispositivos Semicondutores, Brazil

Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli

Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.  Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins.  Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets