Spatially and Spectrally Resolved Hydrogen Gas within 0.1 AU of T Tauri and Herbig Ae/Be Stars

We present near-infrared observations of T Tauri and Herbig Ae/Be stars with a spatial resolution of a few milli-arcseconds and a spectral resolution of ~2000. Our observations spatially resolve gas and dust in the inner regions of protoplanetary disks, and spectrally resolve broad-linewidth emission from the Brackett gamma transition of hydrogen gas. We use the technique of spectro-astrometry to determine centroids of different velocity components of this gaseous emission at a precision orders of magnitude better than the angular resolution. In all sources, we find the gaseous emission to be more compact than or distributed on similar spatial scales to the dust emission. We attempt to fit the data with models including both dust and Brackett gamma-emitting gas, and we consider both disk and infall/outflow morphologies for the gaseous matter. In most cases where we can distinguish between these two models, the data show a preference for infall/outflow models. In all cases, our data appear consistent with the presence of some gas at stellocentric radii of ~0.01 AU. Our findings support the hypothesis that Brackett gamma emission generally traces magnetospherically driven accretion and/or outflows in young star/disk systems.Comment: 48 pages, including 17 figures. Accepted for publication by Ap

Spatially Resolved Spectroscopy of Sub-AU-Sized Regions of T Tauri and Herbig Ae/Be Disks

We present spatially resolved near-IR spectroscopic observations of 15 young stars. Using a grism spectrometer behind the Keck Interferometer, we obtained an angular resolution of a few milli-arcseconds and a spectral resolution of 230, enabling probes of both gas and dust in the inner disks surrounding the target stars. We find that the angular size of the near-IR emission typically increases with wavelength, indicating hot, presumably gaseous material within the dust sublimation radius. Our data also clearly indicate Brackett-gamma emission arising from hot hydrogen gas, and suggest the presence of water vapor and carbon monoxide gas in the inner disks of several objects. This gaseous emission is more compact than the dust continuum emission in all cases. We construct simple physical models of the inner disk and fit them to our data to constrain the spatial distribution and temperature of dust and gas emission components.Comment: 40 pages, 8 figures. Accepted for publication in Ap

Palynological Analysis of a Peat Core from Imnavait Creek, the North Slope, Alaska

The pollen record of a 160 cm peat core from Imnavait Creek, a small upland basin in the northern foothills of the Brooks Range, Alaska, reveals a history of vegetation change from the early Holocene to the present. The Alnus rise within the region occurred after 8500 yr B.P. Betula and Cyperaceae are the major floristic elements throughout the diagram, and this, along with the significant levels of Salix and Ericaceae, suggest that the area was characterized by a mosaic of herb and shrub tundra communities, dependent on variations in terrain and moisture availability.Key words: palynology, Peat , North Slope, Alaska, pollen, Brooks Range, vegetation history, HoloceneMots clés: “Paleontologie”, tourbière, pente nord, Alaska, pollen, Brooks Range, histoire de la végétation, holocèn

Man Versus Food: An Analysis of \u27Dude Food\u27 Television and Public Health

Contemporary food television has moved away from an instructional and cooking-centered model, to an entertainment and eating-focused one. However, public health researchers have not considered these shows in the wake of rising obesity rates. This thesis is concerned with the kinds of messages food television conveys about food and eating and is guided by three research questions: How are food and eating represented on the shows? How is health addressed? How do these shows work to create and promote a more acceptable popular discourse around unhealthy eating habits? Through an analysis of Diners, Drive-Ins and Dives, You Gotta Eat Here! and Man vs. Food Nation, I argue that these food shows downplay or reject entirely health concerns and provide conflicting information about food and health. Finally, by framing these shows within current public health debates on obesity, I discuss possible ways to better inform viewers about the health merits of foods featured on these shows

First L-band Interferometric Observations of a Young Stellar Object: Probing the Circumstellar Environment of MWC 419

We present spatially-resolved K- and L-band spectra (at spectral resolution R = 230 and R = 60, respectively) of MWC 419, a Herbig Ae/Be star. The data were obtained simultaneously with a new configuration of the 85-m baseline Keck Interferometer. Our observations are sensitive to the radial distribution of temperature in the inner region of the disk of MWC 419. We fit the visibility data with both simple geometric and more physical disk models. The geometric models (uniform disk and Gaussian) show that the apparent size increases linearly with wavelength in the 2-4 microns wavelength region, suggesting that the disk is extended with a temperature gradient. A model having a power-law temperature gradient with radius simultaneously fits our interferometric measurements and the spectral energy distribution data from the literature. The slope of the power-law is close to that expected from an optically thick disk. Our spectrally dispersed interferometric measurements include the Br gamma emission line. The measured disk size at and around Br gamma suggests that emitting hydrogen gas is located inside (or within the inner regions) of the dust disk.Comment: Accepted for publication in Ap

Interferometric Observations of V838 Monocerotis

We have used long-baseline near-IR interferometry to resolve the peculiar eruptive variable V838 Mon and to provide the first direct measurement of its angular size. Assuming a uniform disk model for the emission we derive an apparent angular diameter at the time of observations (November-December 2004) of $1.83 \pm 0.06$ milli-arcseconds. For a nominal distance of $8\pm2$ kpc, this implies a linear radius of $1570 \pm 400 R_{\odot}$. However, the data are somewhat better fit by elliptical disk or binary component models, and we suggest that the emission may be strongly affected by ejecta from the outburst.Comment: 12 pages, 1 two-part encapsulated postscript figure. Accepted by ApJL. Added a table of observation

Outflow 20--2000 AU from a High-Mass Protostar in W51-IRS2

We present the results of the first high angular resolution observations of SiO maser emission towards the star forming region W51-IRS2 made with the Very Large Array (VLA) and Very Long Baseline Array (VLBA). Our images of the water maser emission in W51-IRS2 reveal two maser complexes bracketing the SiO maser source. One of these water maser complexes appears to trace a bow shock whose opening angle is consistent with the opening angle observed in the distribution of SiO maser emission. A comparison of our water maser image with an image constructed from data acquired 19 years earlier clearly shows the persistence and motion of this bow shock. The proper motions correspond to an outflow velocity of 80 km/s, which is consistent with the data of 19 years ago (that spanned 2 years). We have discovered a two-armed linear structure in the SiO maser emission on scales of ~25 AU, and we find a velocity gradient on the order of 0.1 km/s/AU along the arms. We propose that the SiO maser source traces the limbs of an accelerating bipolar outflow close to an obscured protostar. We estimate that the outflow makes an angle of <20 degrees with respect to the plane of the sky. Our measurement of the acceleration is consistent with a reported drift in the line-of-sight velocity of the W51 SiO maser source.Comment: 19 pages, 5 figures (including 3 color). Accepted for publication in ApJ (April 1, 2001 issue

Transcriptomic and ChIP-sequence interrogation of EGFR signaling in HER2+ breast cancer cells reveals a dynamic chromatin landscape and S100 genes as targets.

BACKGROUND:The Human Epidermal Growth Factor Receptor (EGFR/HER1) can be activated by several ligands including Transforming Growth Factor alpha (TGF-α) and Epidermal Growth Factor (EGF). Following ligand binding, EGFR heterodimerizes with other HER family members, such as HER2 (human epidermal growth factor receptor-2). Previously, we showed that the EGFR is upregulated in trastuzumab resistant HER2 positive (HER2+) breast cancer cells. This study is aimed to determine the downstream effects on transcription following EGFR upregulation in HER2+ breast cancer cells. METHODS:RNA-sequence and ChIP-sequence for H3K18ac and H3K27ac (Histone H3 lysine K18 and K27 acetylation) were conducted following an Epidermal Growth Factor (EGF) treatment time course in HER2+ breast cancer cells, SKBR3. The levels of several proteins of interest were confirmed by western blot analysis. The cellular localization of proteins of interest was examined using biochemically fractionated lysates followed by western blot analysis. RESULTS:Over the course of 24 h, EGFR stimulation resulted in the modulation of over 4000 transcripts. Moreover, our data demonstrates that EGFR/HER2 signaling regulates the epigenome, with global H3K18ac and H3K27ac oscillating as a function of time following EGF treatment. RNA-sequence data demonstrates the activation of immediate early genes (IEGs) and delayed early genes (DEGs) within 1 h of EGF treatment. More importantly, we have identified members of the S100 (S100 Calcium Binding Protein) gene family as likely direct targets of EGFR signaling as H3K18ac, H3K27ac and pol2 (RNA polymerase II) increase near the transcription start sites of some of these genes. CONCLUSIONS:Our data suggests that S100 proteins, which act as Ca2+ sensors, could play a role in EGF induced tumor cell growth and metastasis, contribute to trastuzumab resistance and cell migration and that they are likely drug targets in HER2+ breast cancer

Holocene Biogeochemical and Pollen History of a Lake Erie, Ohio, Coastal Wetland

Author Institution: Department of Biological and Environmental Sciences, Morehead State University and Byrd Polar Research Center, The Ohio State UniversityA five meter sediment core was taken from Old Woman Creek National Estuarine Research Reserve and State Natural Area and Preserve along the western basin of Lake Erie, U.S.A., to determine the historical biogeochemistry of the wetland. Analysis of pollen and sediment chemistry revealed that the area has remained a wetland since ca. 5,500 yr BP, despite changing lake levels. Pollen stratigraphy indicates a distinctive local succession, which has been divided into three zones. Sediments from sometime after glaciation to 5,500 yr BP were characterized by low concentrations of herb pollen; the next zone showed an establishment of hardwood forest vegetation. The modern local vegetation developed after deforestation (about 200 years ago), when sedimentation increased an order of magnitude, phosphorus deposition increased, and the ecosystem changed from a macrophyte dominated wetland to a plankton dominated marsh. After European settlement, the wetland retained its ability to act as a sink and biotic transformer of bioavailable phosphorus; however, abiotic processes seemed to be more important than the biotic transformations that dominated before deforestation