Immunohistochemical study of PrPSc distribution in neural and extraneural tissues of two cats with feline spongiform encephalopathy

<p>Abstract</p> <p>Background</p> <p>Two domestic shorthair cats presenting with progressive hind-limb ataxia and increased aggressiveness were necropsied and a post mortem diagnosis of Feline Spongiform Encephalopathy (FSE) was made. A wide spectrum of tissue samples was collected and evaluated histologically and immunohistologically for the presence of PrP^Sc.</p> <p>Results</p> <p>Histopathological examination revealed a diffuse vacuolation of the grey matter neuropil with the following areas being most severely affected: corpus geniculatum medialis, thalamus, gyrus dentatus of the hippocampus, corpus striatum, and deep layers of the cerebral and cerebellar cortex as well as in the brain stem. In addition, a diffuse glial reaction involving astrocytes and microglia and intraneuronal vacuolation in a few neurons in the brain stem was present.</p> <p>Heavy PrP^Scimmunostaining was detected in brain, retina, optic nerve, pars nervosa of the pituitary gland, trigeminal ganglia and small amounts in the myenteric plexus of the small intestine (duodenum, jejunum) and slightly in the medulla of the adrenal gland.</p> <p>Conclusion</p> <p>The PrP^Scdistribution within the brain was consistent with that described in other FSE-affected cats. The pattern of abnormal PrP in the retina corresponded to that found in a captive cheetah with FSE, in sheep with scrapie and was similar to nvCJD in humans.</p

Claiming Space and Struggling for Recognition Partispace Working Paper Two

Aiming at contributing to a better understanding of the current developments, paradoxes and ways to deal with youth political participation in Europe, the main research question of the PARTISPACE project is: How and where do 15- to 30 year-old young people participate differently across social milieus and youth cultural scenes and across eight European cities (framed by different national welfare, education and youth policies)? In relation to this research question, this report was intended to analyse case studies which explore participation practices in formal, non-formal and informal contexts and reconstruct practices, meanings, relationship and dynamics of different forms of youth participation in a comparative perspective and local constellations of youth participation. In so doing, the report has outlined a mosaic of the different practices, activities, styles and spaces wherein young people situate their participation in the eight countries involved in the PARTISPACE project

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging.

Fluorescence microscopy is used extensively in cell-biological and biomedical research, but it is often plagued by three major problems with the presently available fluorescent probes: photobleaching, blinking, and large size. We have addressed these problems, with special attention to single-molecule imaging, by developing biocompatible, red-emitting silicon nanocrystals (SiNCs) with a 4.1-nm hydrodynamic diameter. Methods for producing SiNCs by simple chemical etching, for hydrophilically coating them, and for conjugating them to biomolecules precisely at a 1:1 ratio have been developed. Single SiNCs neither blinked nor photobleached during a 300-min overall period observed at video rate. Single receptor molecules in the plasma membrane of living cells (using transferrin receptor) were imaged for ≥10 times longer than with other probes, making it possible for the first time to observe the internalization process of receptor molecules at the single-molecule level. Spatial variations of molecular diffusivity in the scale of 1-2 µm, i.e., a higher level of domain mosaicism in the plasma membrane, were revealed

Co-producing a Research Agenda for Sustainable Palm Oil

The rise of palm oil as the world’s most consumed vegetable oil has coincided with exponential growth in palm oil research activity. Bibliometric analysis of research outputs reveals a distinct imbalance in the type of research being undertaken, notably a disproportionate focus on biofuel and engineering topics. Recognizing the expansion of oil palm agriculture across the tropics and the increasing awareness of environmental, social, and economic impacts, we seek to reorientate the existing research agenda toward one that addresses the most fundamental and urgent questions defined by the palm oil stakeholder community. Following consultation with 659 stakeholders from 38 countries, including palm oil growers, government agencies, non-governmental organizations, and researchers, the highest priority research questions were identified within 13 themes. The resulting 279 questions, including 26 ranked as top priority, reveal a diversity of environmental and social research challenges facing the industry, ranging from the ecological and ecosystem impacts of production, to the livelihoods of plantation workers and smallholder communities. Analysis of the knowledge type produced from these questions underscores a clear need for fundamental science programmes, and studies that involve the consultation of non-academic stakeholders to develop “transformative” solutions to the oil palm sector. Stakeholders were most aligned in their choice of priority questions across the themes of policy and certification related themes, and differed the most in environmental feedback, technology and smallholder related themes. Our recommendations include improved regional academic leadership and coordination, greater engagement with private and public stakeholders in Africa, and Central and South America, and enhanced collaborative efforts with researchers in the major consuming countries of India and China.The online survey and focus groups were funded by the Geran Kursi Endowmen MPOB-UKM Malaysia, and the Royal Geographical Society UK. The residential workshop was supported from by British Council and Academy Science Malaysia via the UK Newton Ungku-Omar Fund. ZD, JB, and MS are supported by the UK Natural Environment Research Council (NE/K016407/1; http://lombok.nerc-hmtf.info/)

Der Immunhistochemische Nachweis von BVD-Virusinfektionen an Hautbiopsien

BVD-Virusinfektionen sind weltweit verbreitet. Diese Viruserkrankung verursacht hohe wirtschaftliche Kosten in der Rinderzucht und wird deshalb in verschiedenen Ländern bekämpft Persistent infizierte Tiere, wie sie nach intrauteriner Infektion entstehen, stellen eine Ansteckungsgefahr für die übrigen Herdentiere dar. Eine geeignete Nachweismethode persistenter BVDV Infektionen ist die Immunhistologie, die sowohl am lebenden wie auch am toten Tier angewendet werden kann. Die Immunhistologie mittels käuflicher monoklonalerAntikörper und Detektions-Kits ist einfach zu handhaben, sehr sensitiv und spezifisch. Für die Diagnose am lebenden Tier eignet sich die Hautbiopsie, am toten Tier sind Organe wie Schilddrüse, Zunge und Gehirn besonders geeignet. BVD-virus infection occur worldwide. This virally induced illness causes high economic losses in cattle reproduction and therefore eradication programms are implemented in various countries. Persistently infected animals are a source of 2 infection for other herdmates. One adequate method for diagnosing persistently infected animals in alive and death animals is the immunohistology. The immunohistology using commercially available monoclonal antibodies and detection kits is easy to perform, highly sensitive and specific. For the diagnosis in living animals a skin biopsy is appropriate, in death animals thyroid gland, tongue and brain are particularly suitable organs

Immunohistochemical diagnosis of persistent infection with Bovine Viral Diarrhea Virus (BVDV) on skin biopsies

Detection of persistent infection with BovineViral Diarrhea Virus (BVDV) is essential for both epidemiological and clinical reasons. In addition to the classical virological methods such as virus isolation in tissue culture, ELISA and RT-PCR, immunohistochemistry of skin biopsies has become a useful and reliable tool. Assuming that the presence of BVDV antigen in skin structures is restricted to persistent infection, this method could differentiate from transient infection. In order to answer this question, 6 calves were experimentally infected orally with a non-cytopathic genotype 1 BVDV strain belonging to the subtype k.The calves developed fever, mucopurulent nasal discharge, coughing and leucopenia with relative lymphopenia. Immunohistochemistry of skin biopsies taken daily up to day 13-post infection did not reveal any evidence of BVDV infection. BVDV was, however, isolated from blood samples on cell cultures. Anti-NS3-antibody-ELISA and serum neutralization tests showed that all six calves seroconverted. We conclude that in acute BVDV infections, with genotype 1 and the subtypes found in Switzerland (b, e, h and k) viral antigen is not found in epidermal structures of the skin. In contrast, persistently infected animals test positive for BVD viral antigen by immunohistochemistry of the skin

Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts

Borna disease is a severe, immunopathological disorder of the central nervous system caused by the infection with the Borna disease virus (BDV). The detection of BDV in diseased animals, mainly sheep and horses, is achieved by histological, immunohistochemical and serological approaches and/or PCR-based technologies. In the present study, reverse transcription, real-time PCR assays were established for the detection of BDV in the brain tissue from sheep and horses, using loci for the p40 (nucleoprotein) and the p24 (phosphoprotein) genes. The PCRs were equally specific and sensitive, detecting 10 target molecules per reaction and one BDV-infected cell among 106 non-infected cells. In tissues from BDV-diseased sheep and horses, the p24 target was detected at higher abundance than for p40. Therefore, the p24 test is suggested to be of higher value in the diagnostic laboratory. However, both assays should be useful for addressing questions in pathogenesis and for detecting BDV reservoirs in endemic areas