Investigating gender differences in the factor structure of the Gudjonsson Compliance Scale

Purpose: The Gudjonsson Compliance Scale (GCS) remains, in terms of its psychometrics, an under-researched instrument, in which gender differences in particular have been insufficiently examined. The aim of this research was to therefore investigate the effect of gender on the factor structure of the GCS. Method: The GCS was administered to 441 females and 250 males. The data were factor-analysed, with 1-, 2-, 3-, and 4-factor solutions tested and compared. Procrustean rotation was applied to the male factor loading matrix to investigate structural equivalence across gender. Results: Although a 3-factor solution was the best fit to the male GCS data, a 4-factor solution was the most acceptable fit to the female data. Whilst each of the factors had a high degree of determinacy, the identity coefficients indicated that these factors differ non-trivially across gender. Conclusion: The GCS may measure different aspects of compliance across males and females, which may explain the gender differences in compliance found within the literature to date. The work also allows insight into why males and females may end up complying with police requests, which might ultimately help to inform strategies, implemented by police, to manage vulnerable general population suspects and witnesses. There is a need now to further investigate the structure of compliance across ethnic groups and/or countries where the GCS is administered

Pancreatic polypeptide inhibits somatostatin secretion

AbstractPancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested, including pancreatic islets, duodenum, hippocampus, and hypothalamus. Its agonism by PP decreases somatostatin secretion from human islets. Mouse embryonic hippocampal (mHippo E18) cells expressed NPY4Rs and their activation by PP consistently decreased somatostatin secretion. Furthermore, central injection of PP in mice induced c-Fos immunoreactivity in somatostatin-containing cells in the hippocampus compared with PBS-injected mice. In sum, our results identify PP as a pivotal modulator of somatostatin secretion

Tissue eosinophilia induced by recombinant human interleukin-5 in the hamster cheek pouch membrane

Interleukin-5 (IL-5) is a cytokine that preferentially effects the development and function of eosinophils, and is considered important in the pathophysiology of allergic inflammation. In this study, we evaluated the ability of recombinant human IL-5 (rHu IL-5) to promote tissue eosinophilia and the importance of this eosinophilia to pathological alterations in vascular function. Repetitive subcutaneous administration for 18 days of rHu IL-5 resulted in a 7-fold increase in the number of eosinophils found in the ipsilateral hamster cheek pouch membrane. The contralateral cheek pouch membrane and peritoneum of these animals showed lesser but significant elevations in the number of eosinophils. In contrast, denatured rHu IL-5 did not elevate eosinophils in these tissues. Through the use of intravital microscopy and fluorometric analysis, rHu IL-5 treated hamster cheek pouch membranes were evaluated for alterations in microvascular permeability, using plasma clearance of FITC-dextran 150 as an index. Despite promoting a prominent tissue eosinophilia, the repetitive subcutaneous injections of rHu IL-5 did not alter the clearance of FITC-dextran 150. Topical application of rHu IL-5 to the cheek pouch, also, had no effect on the clearance of FITC-dextran 150. Immunofluorescence observations using an antibody to the granule protein, eosinophil peroxidase, indicated that the recruited cells had not degranulated. Our results support the importance of IL-5 in the recruitment of tissue eosinophils, but further stimulation is probably required to cause degranulation of these cells and the ensuing tissue damage

Functional Alignment of Regulatory Networks: A Study of Temperate Phages

The relationship between the design and functionality of molecular networks is now a key issue in biology. Comparison of regulatory networks performing similar tasks can provide insights into how network architecture is constrained by the functions it directs. Here, we discuss methods of network comparison based on network architecture and signaling logic. Introducing local and global signaling scores for the difference between two networks, we quantify similarities between evolutionarily closely and distantly related bacteriophages. Despite the large evolutionary separation between phage λ and 186, their networks are found to be similar when difference is measured in terms of global signaling. We finally discuss how network alignment can be used to pinpoint protein similarities viewed from the network perspective

Anterior Ischemic Optic Neuropathy Associated with Udenafil

We report a case of anterior ischemic optic neuropathy associated with udenafil. A 54-year-old male presented with an acute onset visual field defect of the right eye after udenafil use. Examination revealed a relative afferent pupillary defect and a swollen disc. Automated visual fields revealed an enlarged blind spot and a narrowed visual field. Fluorescein angiography revealed both an inferior choroidal filling delay and an inferior sector filling delay of the optic disc in the arteriovenous phase as well as diffuse leakage of the optic disc in the late phase. Optical coherent tomography revealed increased thickness of the retinal nerve fiber layer, especially in the area of the inferior disc. The patient was counseled to discontinue the use of udenafil and to monitor his blood pressure regularly. The disc swelling was resolved with residual optic atrophy one month after discontinuing the use of udenafil

Social network analysis of white-tailed deer scraping behavior: Implications for disease transmission

Host contact structure affects pathogen transmission in host populations, but many measures of host contact do not distinguish contacts that are relevant to pathogen transmission from those that are not. Scrapes are sites for chemical communication by white-tailed deer (Odocoileus virginianus) during the breeding season and potential sites of transmission of prions, the causative agent of chronic wasting disease (CWD). Scrape-related behaviors vary in their probability of transmitting prions to or from the environment, suggesting that behavior be combined with contact structure to better reflect potential heterogeneity in prion transmission at scrapes. We recorded visits and behaviors by deer at scrapes throughout DeSoto National Wildlife Refuge, Nebraska in 2005 and 2006. We recorded 2013 interactions by 169 unique identifiable males and 75 females. Adult males performed the most scrape-related behaviors and spent the most time at scrapes, especially smelling the overhanging branch (70%), smelling the scrape (59%), licking/grasping the overhanging branch (44%), and scraping (36%). We used social network analysis to test the effect of behavior on indirect contact networks among deer at scrapes. By weighting edges based on the frequency and duration of behaviors, we produced networks representing sources of variation in scrape use and compared these networks to evaluate the effects of behavior on network contact structure. Social networks based on scrape-related behavior were highly connected and dependent upon the frequency, duration, and type of behavior exhibited at scrapes (e.g., scraping, interacting with a scrape or overhanging branch, rub-urinating, grazing) as well as the age of the deer. Accounting for contact frequency produced networks with lower variation in contact, but higher ability to facilitate contact among disparate groups. Including behavior when defining edges did not preserve the network properties of simpler measures (i.e., unweighted networks) suggesting that heterogeneity in behaviors that affect transmission probability is important for inferring transmission networks from contact networks. High connectivity through indirect contacts suggests that scrapes may be effective targets for management. Adult male deer had the highest connectivity, suggesting that management strategies focused on reducing their interaction with scrapes through density reduction or behavioral modification could reduce the connectivity of indirect contact networks

The effect of cognitive load on faking interrogative suggestibility on the Gudjonsson Suggestibility Scale

In the light of recent studies into the impact of cognitive load on detecting deception, the impact of cognitive load on faking on the Gudjonsson Suggestibility Scale (GSS) was investigated. Eighty undergraduate students participated in the study, and were randomly assigned to one of four conditions resulting from a combination of the factors: instruction type (genuine or instructed faking, see Hansen, Smeets, & Jelicic, 2009) and concurrent task (yes or no). Findings show that instructed fakers, not performing a concurrent task, score significantly higher on yield 1 in comparison to genuine interviewees. This is in line with previous studies into faking on the GSS. However, instructed fakers, performing a concurrent task, achieved significantly lower yield 1 scores than instructed fakers not performing a concurrent task. Genuine (non fakers) showed a different response to increased cognitive load during the dual-task paradigm. This study suggests that increasing cognitive load may potentially indicate (and preclude) faking attempts on the yield dimension of the Gudjonsson Suggestibility Scale

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a special plasmid, pREDI, which carries two independent inducible promoters: (i) an arabinose-inducible promoter that drives expression of λ-Red recombination proteins, which carry out the replacement of a target genomic region with a marker-containing linear DNA cassette, and (ii) a rhamnose-inducible promoter that drives expression of I-SceI endonuclease, which stimulates deletion of the introduced marker by double-strand breakage-mediated intramolecular recombination. This genomic deletion was performed successively with only one plasmid, pREDI, simply by changing the carbon source in the bacterial growth medium from arabinose to rhamnose. The efficiencies of targeted region replacement and deletion of the inserted linear DNA cassette were nearly 70 and 100%, respectively. This rapid and efficient procedure can be adapted for use in generating a variety of genome modifications

Mouse skeletal muscle fiber-type-specific macroautophagy and muscle wasting are regulated by a Fyn/STAT3/Vps34 signaling pathway

Skeletal muscle atrophy induced by aging (sarcopenia), inactivity, and prolonged fasting states (starvation) is predominantly restricted to glycolytic type II muscle fibers and typical spares oxidative type I fibers. However, the mechanisms accounting for muscle fiber-type specificity of atrophy have remained enigmatic. In the current study, although the Fyn tyrosine kinase activated the mTORC1 signaling complex, it also induced marked atrophy of glycolytic fibers with relatively less effect on oxidative muscle fibers. This was due to inhibition of macroautophagy via an mTORC1-independent but STAT3-dependent reduction in Vps34 protein levels and decreased Vps34/p150/Beclin1/Atg14 complex 1. Physiologically, in the fed state endogenous Fyn kinase activity was increased in glycolytic but not oxidative skeletal muscle. In parallel, Y705-STAT3 phosphorylation increased with decreased Vps34 protein levels. Moreover, fed/starved regulation of Y705-STAT3 phosphorylation and Vps34 protein levels was prevented in skeletal muscle of Fyn null mice. These data demonstrate a Fyn/STAT3/Vps34 pathway that is responsible for fiber-type-specific regulation of macroautophagy and skeletal muscle atrophy