665 research outputs found
Polymerase I and Transcript Release Factor Regulates Lipolysis via a Phosphorylation-Dependent Mechanism
OBJECTIVE: Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization. RESEARCH DESIGN AND METHODS: PTRF expression was examined in different adipose depots of mice during fasting, refeeding, and after administration of catecholamines and insulin. Involvement of PTRF during lipolysis was studied upon PTRF knockdown and overexpression and mutation of PTRF phosphorylation sites in 3T3-L1 adipocytes. RESULTS: PTRF expression in mouse white adipose tissue (WAT) is regulated by nutritional status, increasing during fasting and decreasing to baseline after refeeding. Expression of PTRF also is hormonally regulated because treatment of mice with insulin leads to a decrease in expression, whereas isoproterenol increases expression in WAT. Manipulation of PTRF levels revealed a role of PTRF in lipolysis. Lentiviral-mediated knockdown of PTRF resulted in a marked attenuation of glycerol release in response to isoproterenol. Conversely, overexpressing PTRF enhanced isoproterenol-stimulated glycerol release. Mass-spectrometric analysis revealed that PTRF is phosphorylated at multiple sites in WAT. Mutation of serine 42, threonine 304, or serine 368 to alanine reduced isoproterenol-stimulated glycerol release in 3T3-L1 adipocytes. CONCLUSIONS: Our study is the first direct demonstration for a novel adipose tissue–specific function of PTRF as a mediator of lipolysis and also shows that phosphorylation of PTRF is required for efficient fat mobilization
Promising Functional Readouts of Immunity in a Blood-Stage Malaria Vaccine Trial
The authors discuss results from an early trial of a vaccine based on Plasmodium MSP-3 protein reported by Pierre Druilhe and colleagues
The Hydrogen Epoch of Reionization Array Dish II: Characterization of Spectral Structure with Electromagnetic Simulations and its science Implications
We use time-domain electromagnetic simulations to determine the spectral
characteristics of the Hydrogen Epoch of Reionization Arrays (HERA) antenna.
These simulations are part of a multi-faceted campaign to determine the
effectiveness of the dish's design for obtaining a detection of redshifted 21
cm emission from the epoch of reionization. Our simulations show the existence
of reflections between HERA's suspended feed and its parabolic dish reflector
that fall below -40 dB at 150 ns and, for reasonable impedance matches, have a
negligible impact on HERA's ability to constrain EoR parameters. It follows
that despite the reflections they introduce, dishes are effective for
increasing the sensitivity of EoR experiments at relatively low cost. We find
that electromagnetic resonances in the HERA feed's cylindrical skirt, which is
intended to reduce cross coupling and beam ellipticity, introduces significant
power at large delays ( dB at 200 ns) which can lead to some loss of
measurable Fourier modes and a modest reduction in sensitivity. Even in the
presence of this structure, we find that the spectral response of the antenna
is sufficiently smooth for delay filtering to contain foreground emission at
line-of-sight wave numbers below Mpc, in
the region where the current PAPER experiment operates. Incorporating these
results into a Fisher Matrix analysis, we find that the spectral structure
observed in our simulations has only a small effect on the tight constraints
HERA can achieve on parameters associated with the astrophysics of
reionization.Comment: Accepted to ApJ, 18 pages, 17 Figures. Replacement matches accepted
manuscrip
Application of in situ process monitoring to optimise laser parameters during laser powder bed fusion printing of Ti-6Al-4V parts with overhang structures
Enhanced levels of alloy print defects such as porosity are associated with the printing of overhang structures by laser powder bed fusion (L-PBF). This study compared the microstructure and porosity of Ti-6Al-4V overhang structures, with that observed for the bulk alloy. It was observed in the region around the overhang structure that the microstructure exhibited larger grain sizes and was less homogenous, compared to the that obtained within the bulk alloy. An increased level of porosity of up to 0.08% was also observed in the overhang print alloy, compared with the corresponding < 0.02% in the alloy bulk. It is hypothesised that these microstructural changes are associated with the excess heat generated in the overhang region, due to the decreased thermal conductivity of the powder immediately below the print layers, compared with solid alloy. During L-PBF alloy printing, in situ process monitoring of the melt pool emissions was obtained in the near-infrared range and correlated with the properties of the printed parts. This in-process data was used to assist in selecting optimal laser processing conditions, in order to help prevent melt pool overheating at the overhang. By systematically controlling the laser energy during the printing of the first fifteen layers over the overhang structure, the level of porosity was reduced, to the < 0.02% level of the bulk alloy. There was also an associated reduction in the roughness (Ra) of the overhang itself, with its Ra decreasing from 62.4 ± 7.3 to 7.5 ± 1.9 µm.Science Foundation IrelandSMART Eureka project APEM-A
A massive cluster of Red Supergiants at the base of the Scutum-Crux arm
We report on the unprecedented Red Supergiant (RSG) population of a massive
young cluster, located at the base of the Scutum-Crux Galactic arm. We identify
candidate cluster RSGs based on {\it 2MASS} photometry and medium resolution
spectroscopy. With follow-up high-resolution spectroscopy, we use CO-bandhead
equivalent width and high-precision radial velocity measurements to identify a
core grouping of 26 physically-associated RSGs -- the largest such cluster
known to-date. Using the stars' velocity dispersion, and their inferred
luminosities in conjuction with evolutionary models, we argue that the cluster
has an initial mass of 40,000\msun, and is therefore among the most
massive in the galaxy. Further, the cluster is only a few hundred parsecs away
from the cluster of 14 RSGs recently reported by Figer et al (2006). These two
RSG clusters represent 20% of all known RSGs in the Galaxy, and now offer the
unique opportunity to study the pre-supernova evolution of massive stars, and
the Blue- to Red-Supergiant ratio at uniform metallicity. We use GLIMPSE,
MIPSGAL and MAGPIS survey data to identify several objects in the field of the
larger cluster which seem to be indicative of recent region-wide starburst
activity at the point where the Scutum-Crux arm intercepts the Galactic bulge.
Future abundance studies of these clusters will therefore permit the study of
the chemical evolution and metallicity gradient of the Galaxy in the region
where the disk meets the bulge.Comment: 49 pages, 22 figures. Accepted for publication in ApJ. Version with
hi-res figures can be found at http://www.cis.rit.edu/~bxdpci/RSGC2.pd
The Hydrogen Epoch of Reionization Array Dish I: Beam Pattern Measurements and Science Implications
The Hydrogen Epoch of Reionization Array (HERA) is a radio interferometer
aiming to detect the power spectrum of 21 cm fluctuations from neutral hydrogen
from the Epoch of Reionization (EOR). Drawing on lessons from the Murchison
Widefield Array (MWA) and the Precision Array for Probing the Epoch of
Reionization (PAPER), HERA is a hexagonal array of large (14 m diameter) dishes
with suspended dipole feeds. Not only does the dish determine overall
sensitivity, it affects the observed frequency structure of foregrounds in the
interferometer. This is the first of a series of four papers characterizing the
frequency and angular response of the dish with simulations and measurements.
We focus in this paper on the angular response (i.e., power pattern), which
sets the relative weighting between sky regions of high and low delay, and
thus, apparent source frequency structure. We measure the angular response at
137 MHz using the ORBCOMM beam mapping system of Neben et al. We measure a
collecting area of 93 m^2 in the optimal dish/feed configuration, implying
HERA-320 should detect the EOR power spectrum at z~9 with a signal-to-noise
ratio of 12.7 using a foreground avoidance approach with a single season of
observations, and 74.3 using a foreground subtraction approach. Lastly we study
the impact of these beam measurements on the distribution of foregrounds in
Fourier space.Comment: 13 pages, 9 figures. Replaced to match accepted ApJ versio
The Bolocam Galactic Plane Survey: Survey Description and Data Reduction
We present the Bolocam Galactic Plane Survey (BGPS), a 1.1 mm continuum
survey at 33" effective resolution of 170 square degrees of the Galactic Plane
visible from the northern hemisphere. The survey is contiguous over the range
-10.5 < l < 90.5, |b| < 0.5 and encompasses 133 square degrees, including some
extended regions |b| < 1.5. In addition to the contiguous region, four targeted
regions in the outer Galaxy were observed: IC1396, a region towards the Perseus
Arm, W3/4/5, and Gem OB1. The BGPS has detected approximately 8400 clumps over
the entire area to a limiting non-uniform 1-sigma noise level in the range 11
to 53 mJy/beam in the inner Galaxy. The BGPS source catalog is presented in a
companion paper (Rosolowsky et al. 2010). This paper details the survey
observations and data reduction methods for the images. We discuss in detail
the determination of astrometric and flux density calibration uncertainties and
compare our results to the literature. Data processing algorithms that separate
astronomical signals from time-variable atmospheric fluctuations in the data
time-stream are presented. These algorithms reproduce the structure of the
astronomical sky over a limited range of angular scales and produce artifacts
in the vicinity of bright sources. Based on simulations, we find that extended
emission on scales larger than about 5.9' is nearly completely attenuated (>
90%) and the linear scale at which the attenuation reaches 50% is 3.8'.
Comparison with other millimeter-wave data sets implies a possible systematic
offset in flux calibration, for which no cause has been discovered. This
presentation serves as a companion and guide to the public data release through
NASA's Infrared Processing and Analysis Center (IPAC) Infrared Science Archive
(IRSA). New data releases will be provided through IPAC IRSA with any future
improvements in the reduction.Comment: Accepted for publication in Astrophysical Journal Supplemen
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