Análise da caracterizaçao da bexiga com o uso do extrato aquoso da Orbignya phalerata (babaçu) : estudo controlado em ratos

Orientador: Osvaldo MalafaiaInclui apendicesDissertaçao (mestrado) - Programa de Mestrado Interinstitucional em Clínica Cirúrgica, Universidade Federal do Paraná e Universidade Federal do Maranhao. Defesa: Curitiba, 2004Inclui bibliografiaÁrea de concentraçao: Clínica cirúrgicaResumo: A cicatrização constitui processo complexo, envolvendo diferentes sistemas biológicos e imunológicos, sendo essencial para manter a integridade do organismo. Três fases bem definidas ocorrem: inflamatória, proliferativa e maturação. Falha ou prolongamento em uma das fases pode resultar em retardo ou ausência da mesma. O homem sempre buscou amenizar seu próprio sofrimento e curar seus males, testando para isso os medicamentos fitoterápicos mesocarpo de babaçu (Orbignya phlalerata) tem sido usado em estudos experimentais, procurando-se verificar sua ação pro-inflamatória. Objetivo - Analisar comparativamente as alterações histológicas proporcionadas pelo uso do extrato aquoso da Orbignya phalerata na cicatrização de lesões cirúrgicas da bexiga. Material e método - Quarenta ratos da linhagem Wistar, adultos e machos foram utilizados. O procedimento experimental constituiu-se em incisão longitudinal de 2cm na bexiga e síntese em plano único com pontos separados de poliglactina 910 (Ethicon) cinco zeros. Apos este procedimento comum, os animais foram distribuídos aleatoriamente em dois grupos contendo vinte animais cada. No grupo-controle não foi utilizada a substancia Orbignya phalerata. No grupo-experimento' utilizou-se solução aquosa de Orbignya phalerata na dose de 50mg/kg por via intraperitoneal. Os animais foram acompanhados e mortos em três e sete dias. Foi feita analise histológica comparativa entre os grupos. Resultados - Foi observada diferença estatística significante nas variáveis neoformação (p= 0,001) e proliferação fibroblastica (p= 0,010) nos subgrupos-controle e experimento do 3o dia. Ainda no grupo-experimento a neoformação capilar foi mais intensa que no grupo-controle, apresentando significância estatística. No grupo de 7° dias observou-se que as variáveis inflamação aguda (p= 0,001) inflamação crônica (p= 0,002) e proliferação fibroblastica (p=0,023) apresentaram significância estatística, sendo bem evidente a inflamação aguda no grupo controle. Conclusão - Foi observada homogeneidade no fator tempo da cicatrização nos grupos-experimento e controle. Contudo, ela foi mais rápida no grupo-experimento. Houve efeito favorecedor cicatrizante na utilização da Orbignya phalerata na bexiga do rato.Abstract: Wound healing is a complex process that deals with different biological and immunological systems and is essential to keep the organism integrity. Three welldefined phases occur: inflammatory, proliferative and maturation. A failure or lengthym phase may result in a delay or absence of it. Man has always sought to ease his own suffering and heal his diseases by the testing of Phytotherapeutic drugs. The babassu mesocarp (Orbignya phalerata) has been used in experimental studies in order to verify its pro-inflammatory action. Objective- To analyse comparatively the histological alteration provided by the use of the aqueous extract of Orbignya phalerata, in the healing of bladder surgical wounds. Material and method- Forty adult, male Wistar rats were used. The experimental procedure consisted of a longitudinal 2cm long bladder incision and single layer interrupted suture of 5-0 poliglactine. Postoperatively, the rats were randomly divided into two groups of twenty. The substance was not used in the control group. The Orbignya phalerata aqueous solution was used in the study group. The animals were observed and killed three and seven days later. Comparative histological analysis was accomplished between the groups. Results- Significant statistical differences were observed in the neo-formation variables (p= 0,001), chronic inflammation (p= 0,002) and fibroblastic proliferation (p= 0,023). Acute inflammation was very evident in the control group. Conclusion- The time factor of wound healing showed homogeneity between study and control groups. However, it was faster in the study group. There was a favoring healing effect of Orbignya phalerata when it was used on the bladder of rats

Seleção de padrões de codigo para sintese de datapaths especializados

Orientadores: Rodolfo Jardim de Azevedo, Guido Costa Souza de AraujoDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Matematica, Estatistica e Computação CientificaMestradoMestre em Matemátic

Progressão da fibrose hepática em portadores de coinfecção HIV/vírus da hepatite C com níveis de aminotransferases normais

INTRODUCTION: Approximately 30% of hepatitis C virus (HCV) monoinfected patients present persistently normal alanine aminotransferase (ALT) levels. Most of these patients have a slow progression of liver fibrosis. Studies have demonstrated the rate of liver fibrosis progression in hepatitis C virus-human immunodeficiency virus (HCV-HIV) coinfected patients is faster than in patients infected only by HCV. Few studies have evaluated the histological features of chronic hepatitis C in HIV-infected patients with normal ALT levels. METHODS: HCV-HIV coinfected patients (HCV-RNA and anti-HIV positive) with known time of HCV infection (intravenous drugs users) were selected. Patients with hepatitis B surface antigen (HBsAg) positive or hepatitis C treatment before liver biopsy were excluded. Patients were considered to have a normal ALT levels if they had at least 3 normal determinations in the previous 6 months prior to liver biopsy. All patients were submitted to liver biopsy and METAVIR scale was used. RESULTS: Of 50 studied patients 40 (80%) were males. All patients were treated with antiretroviral therapy. The ALT levels were normal in 13 (26%) patients. HCV-HIV co-infected patients with normal ALT levels had presented means of the liver fibrosis stages (0.77±0.44 versus 1.86±1.38; p<0.001) periportal inflammatory activity (0.62±0.77 versus 2.24±1.35; p<0.001) and liver fibrosis progression rate (0.058±0.043 fibrosis unit/year versus 0.118±0.102 fibrosis unit/year) significantly lower as compared to those with elevated ALT. CONCLUSIONS: HCV-HIV coinfected patients with persistently normal ALTs showed slower progression of liver fibrosis. In these patients the development of liver cirrhosis is improbable.INTRODUÇÃO: Aproximadamente, 30% dos portadores de hepatite crônica C apresentam níveis de aminotransferases persistentemente normais (APNL). A maioria destes pacientes tem lenta progressão da fibrose hepática. Em portadores de coinfecção VHC-HIV, estudos têm demonstrado que a progressão da fibrose hepática é mais rápida que a observada em indivíduos infectados somente pelo VHC. Há poucos estudos que verificaram as características histológicas da hepatite crônica C em pacientes coinfectados pelo HIV APNL. MÉTODOS: Portadores de coinfecção VHC-HIV (HCV-RNA e anti-HIV positivos) com tempo de infecção pelo VHC conhecido (uso de drogas intravenosas) foram selecionados. Aqueles com hepatitis B surface antigen (HbsAg) positivo ou que tenham sido submetidos à terapia antiviral para hepatite C antes da biópsia hepática foram excluídos. Pacientes com pelo menos 3 determinações normais da ALT nos últimos 6 meses antes da biópsia hepática foram considerados como tendo APNL. Todos foram submetidos a biópsia hepática que foi classificada de acordo com a escala METAVIR. RESULTADOS: Foram incluídos 50 pacientes, 40 (80%) homens. Todos receberam terapia antirretroviral. Os níveis de ALT foram persistentemente normais em 13 (26%) pacientes. Pacientes coinfectados com APNL apresentaram menor média dos estágiosde fibrose hepática (0,77±0,44 versus 1,86±1,38; p<0,001), dos índices de atividade inflamatória periportal (0,62±0,77 versus 2,24±1,35; p<0,001) e progressão mais lenta da fibrose hepática (0,058±0,043 unidades de fibrose /ano versus 0,118±0,102 unidades de fibrose/ano) quando comparados àqueles com aminotransferases elevadas. CONCLUSÕES: Portadores de coinfecção VHC-HIV com APNL apresentam progressão mais lenta da fibrose hepática. Nesses pacientes o desenvolvimento de cirrose hepática é improvável.Universidade Federal de Juiz de Fora Centro de Referência em Hepatologia Departamento de GastroenterologiaUniversidade Federal de Juiz de Fora Unidade de Endoscopia DigestivaUniversidade Federal de São Paulo (UNIFESP) Divisão de Gastroenterologia Seção de HepatitesUNIFESP, Divisão de Gastroenterologia Seção de HepatitesSciEL

Calidad de vida relacionada con la salud en pacientes con hepatitis c en terapia doble y triple

OBJETIVO Comparar los puntajes de Calidad de Vida Relacionada con la Salud (CVRS) en pacientes con hepatitis C crónica sometidos a la terapia antiviral doble y triple y analizar los posibles factores relacionados con la CVRS. MÉTODO La CVRS fue evaluada utilizando el Short Form 36 y elChronic Liver Disease Questionnaire , que fueron aplicados antes y en las semanas 4, 12 y 16 de tratamiento, en 32 pacientes divididos en 2 grupos: terapia doble con interferón pegilado (IFN-PEG) y ribavirina, y triple con IFN-PEG, ribavirina y telaprevir. RESULTADOS La reducción de la CVRS fue mayor en pacientes en terapia triple cuando comparados con los tratados con dos drogas, siendo el momento más crítico la 12ª semana en ambos grupos. Después de la retirada del telaprevir, el grupo de terapia triple mejoró de modo significativo los puntajes de CVRS. Ansiedad y depresión en el pre tratamiento, situación de empleo y raza se mostraron relacionados con la reducción de la CVRS. CONCLUSIÓN Pacientes sometidos a la terapia doble y triple presentan reducción de los índices de CVRS, pero la adición del telaprevir les proporciona una caída más expresiva.OBJETIVO Comparar os escores de Qualidade de Vida Relacionada à Saúde (QVRS) em pacientes com hepatite crônica C submetidos à terapia antiviral dupla e tripla e analisar os possíveis fatores relacionados à QVRS. MÉTODO A QVRS foi avaliada utilizando o Short Form 36 e oChronic Liver Disease Questionnaire , que foram aplicados antes e nas semanas 4, 12 e 16 de tratamento, em 32 pacientes divididos em 2 grupos: terapia dupla com interferon peguilado (IFN-PEG) e ribavirina e tripla com IFN-PEG, ribavirina e telaprevir. RESULTADOS A redução da QVRS foi maior em pacientes em terapia tripla quando comparados àqueles tratados com duas drogas, sendo o momento mais crítico a 12ª semana em ambos os grupos. Após a retirada do telaprevir, o grupo terapia tripla melhorou de modo significativo os escores de QVRS. Ansiedade e depressão no pré-tratamento, status empregatício e raça se mostraram relacionados à redução da QVRS. CONCLUSÃO Pacientes submetidos à terapia dupla e tripla apresentam redução dos índices de QVRS, mas a adição do telaprevir confere uma queda mais expressiva.OBJECTIVE Comparing Health-Related Quality of Life (HRQoL) scores in patients with chronic hepatitis C undergoing double and triple antiviral therapy and analyzing possible factors related to HRQoL. METHOD HRQoL was assessed using the Short Form 36 and Chronic Liver Disease Questionnaire, which were applied at baseline and at weeks 4, 12 and 16 of treatment to 32 patients divided into two groups: double therapy with pegylated interferon (IFN-PEG) and ribavirin, and triple therapy with PEG-IFN, ribavirin and telaprevir. RESULTS The reduction of HRQoL was greater in patients receiving triple therapy compared to those treated with two drugs, the most critical time is at 12 weeks in both groups. After removal of telaprevir, the triple therapy group significantly improved their HRQoL scores. Anxiety and depression before treatment, employment status and race are significantly related to diminished HRQoL. CONCLUSION Patients undergoing double and triple therapy have diminished HRQoL indexes, but the addition of telaprevir chooses a more significant decrease

Zingiber zerumbet flower stem postharvest characterization

About the Zingiber zerumbet little is known about its cut flower postharvest and market, despite its high ornamental potential. The inflorescences, which resemble a compact cone, emerge from the base of the plants and start with green color changing to red with the age. This study objective was to characterize floral stem of ornamental ginger in two cultivate conditions and to evaluate the longevity of those submitted to post-harvest treatments. Flower stems were harvest from clumps cultivated under full sun and partial shade area, and were submitted to the postharvest treatments: complete flower immersion in tap water (CFI) or only the base stem immersion (BSI). The flower stems harvested from clumps at partial shade presented higher fresh weight, length and diameter of the inflorescences compared to flower stems harvested from clumps at full sun area. The flower stem bracts cultivated in full sun area changed the color from green to red 10.69 and 11.94 days after BSI and CFI postharvest treatments, and the vase life were 22.94 and 28.19 days, respectively. Flower stem harvest in partial shade area change the color only after 18.94 and 18.43 days and the vase life durability was 27.56 and 31.81, respectively. The complete immersion of the flower stem increase the vase life durability in 5.25 and 4.25 days compared to flowers kept with the stem base immersed only, in flower stems harvested from clumps cultivated in full sun area and partial shade area, respectively. Flower stems harvested from clumps cultivated in partial shade area and completely immerse in tap water during 3 hours increase the vase life durability in 8.87 days compared to flowers harvested from clumps cultivated in full sun area and base immersed only

Morphophenological characterization of ornamental ginger and selection for landscape use

Many species of Zingiber have great ornamental potential, due to durability and exotic appearance of the inflorescences. Despite its large phenotypic variability, they are scarcely exploited or not yet exploited regarding the ornamental potential. To conserve potential ornamental genotypes, and subsidize breeding program, the Agronomic Institute (IAC) maintain a Germoplasm Collection of Ornamental Zingiberales with promising accessions, including Zingiber. The aim was the morphophenological characterization of ten Zingiber accessions and the indication for landscape purposes. A large variation was observed to the evaluated characters: Clump height (CH); Inflorescence visualization (IV); Clump area (CA); Clump density (CD); Leaf stem Firmness (LSF); Number of leaf stems per clump (NLSC); Number of leaves per stem (NLS); Leaf color (LCol); Evergreen tendency (ET); Flower stem growth (FSG); Flower stem length (FSLe); Flower stem diameter (FSD); Flower stem per clump (FSC); Color sensorial perception (CSP); Flower stem weight (FSW); Inflorescence length (IL); Inflorescence diameter (ID); Bracts aspects (BAs); and Flowering season (FSe). The accessions very suitable and with the best performance to use for landscape purpose were Z. spectabile, IAC Anchieta (Z. spectabile), Z. newmanii

Leishmania infantum AS A CAUSATIVE AGENT OF CUTANEOUS LEISHMANIASIS IN THE STATE OF MATO GROSSO DO SUL, BRAZIL

Cutaneous leishmaniasis is caused by different species of theLeishmania genus. Leishmania(Leishmania) infantum, causing cutaneous leishmaniasis, has been described in patients living in areas where visceral leishmaniasis is endemic. In this study, it was possible to characterize this species in seven slides from cutaneous tissue imprints from patients with cutaneous leishmaniasis in the State of Mato Grosso do Sul, Brazil

Dermatology relevance to graduates from the Universidade Estadual de Campinas Medical School

Some research indicates that physicians do not dominate the expected dermatological content for the proper exercise of the profession. This fact compromises their diagnostic and therapeutic performance, generating unnecessary costs. OBJECTIVE: The aim of this study was to evaluate the relevance of Dermatology and the knowledge acquired in the specialty during the undergraduate course in clinical practice of graduates at the State University of Campinas Medical School (FCM/UNICAMP). METHOD: A questionnaire with 22 closed questions and two open ones was electronically sent to physicians who had graduated more than 10 years ago and others for less than 10 years. In the first group, physicians were trained by the same curriculum and in the second group there were subjects trained by the old and the new curriculum. RESULTS: Of the 126 respondents, 83% had completed a specialization course. Among all, 82% did not study dermatology after graduation. The majority considered that Dermatology has high relevance in clinical practice, regardless of the group. There was a statistically significant difference between non-dermatologist doctors graduated for more than 10 years and those graduated for less than 10 years regarding confidence about lesion diagnosis, diagnostic investigation and treatment of skin diseases. Physicians who have graduated for a longer time feel more insecure in relation to patients with dermatoses. Concerning contributions offered by graduation program completion they prioritized outpatient care, ability to diagnose, knowledge of pathology, research and knowledge of lesions. CONCLUSION: This study has shown that Dermatology is relevant in medical practice and more recent graduates from the FCM/UNICAMP feel less insecure when treating a patient with dermatoses90563163