On the Unicity of Smartphone Applications

Prior works have shown that the list of apps installed by a user reveal a lot about user interests and behavior. These works rely on the semantics of the installed apps and show that various user traits could be learnt automatically using off-the-shelf machine-learning techniques. In this work, we focus on the re-identifiability issue and thoroughly study the unicity of smartphone apps on a dataset containing 54,893 Android users collected over a period of 7 months. Our study finds that any 4 apps installed by a user are enough (more than 95% times) for the re-identification of the user in our dataset. As the complete list of installed apps is unique for 99% of the users in our dataset, it can be easily used to track/profile the users by a service such as Twitter that has access to the whole list of installed apps of users. As our analyzed dataset is small as compared to the total population of Android users, we also study how unicity would vary with larger datasets. This work emphasizes the need of better privacy guards against collection, use and release of the list of installed apps.Comment: 10 pages, 9 Figures, Appeared at ACM CCS Workshop on Privacy in Electronic Society (WPES) 201

Quantification of vaporized targeted nanodroplets using high-frame-rate ultrasound and optics

Owing to their ability to efficiently deliver biological cargo and sense the intracellular milieu, vertical arrays of high aspect ratio nanostructures, known as nanoneedles,are being developed as minimally invasive tools for cell manipulation. However, little is known of the mechanisms of cargo transfer across the cell membrane-nanoneedle interface. Particularly,the contributions of membrane piercing, modulation of membrane permeability and endocytosis to cargo transfer remain largelyunexplored. Here, combining state-of-the-art electron and scanning ion conductance microscopy with molecular biology techniques, we show that porous silicon nanoneedle arrays concurrently stimulate independent endocytic pathways which contribute to enhanced biomolecule delivery into human mesenchymal stem cells. Electron microscopy of the cell membrane at nanoneedle sites shows an intact lipid bilayer, accompanied by an accumulation of clathrin-coated pits and caveolae. Nanoneedles enhance the internalisation of biomolecular markers of endocytosis, highlighting the concurrent activation of caveolae-and clathrin-mediated endocytosis, alongside macropinocytosis. These events contribute to the nanoneedle-mediated delivery (nanoinjection) of nucleic acids into human stem cells, which distribute across the cytosol and the endolysosomal system. This data extends the understanding of how nanoneedles modulate biological processes to mediate interaction with the intracellular space, providing indications for the rational design of improved cell-manipulation technologies

In search of green political economy: steering markets, innovation and the case of the zero carbon homes agenda in England

Advocates of a democratic ‘Green state’ challenge Hayekian free-market environmentalist proposals for a minimal state and the emphasis of ecological modernisation discourses on technological innovation as the primary route towards ecological sustainability. However, these more strongly pro-market traditions raise important questions and provide useful insights concerning the challenges of translating the political ideology of ‘ecologism’ into practical proposals for democratic governance. Hayekian thought raises vital questions concerning the capacity of political processes to address complex challenges of coordinating the formulation and delivery of the sustainability objectives of ecologism. Scholarship on ecological modernisation and the ‘new regulation’ offer important insights into how shifting interrelationships between the state and private sector in the policy process might enable this challenge to be more effectively addressed. These areas for further developing proposals for a Green state are illustrated here through a case study of the zero carbon homes policy agenda in England

Peptide location fingerprinting reveals tissue region-specific differences in protein structures in an ageing human organ

In ageing tissues, long-lived extracellular matrix (ECM) proteins are susceptible to the accumulation of structural damage due to diverse mechanisms including glycation, oxidation and protease cleavage. Peptide location fingerprinting (PLF) is a new mass spectrometry (MS) analysis technique capable of identifying proteins exhibiting structural differences in complex proteomes. PLF applied to published young and aged intervertebral disc (IVD) MS datasets (posterior, lateral and anterior regions of the annulus fibrosus) identified 268 proteins with age-associated structural differences. For several ECM assemblies (collagens I, II and V and aggrecan), these differences were markedly conserved between degeneration-prone (posterior and lateral) and -resistant (anterior) regions. Significant differences in peptide yields, observed within collagen I alpha 2, collagen II alpha 1 and collagen V alpha 1, were located within their triple-helical regions and/or cleaved C-terminal propeptides, indicating potential accumulation of damage and impaired maintenance. Several proteins (collagen V alpha 1, collagen II alpha 1 and aggrecan) also exhibited tissue region (lateral)-specific differences in structure between aged and young samples, suggesting that some ageing mechanisms may act locally within tissues. This study not only reveals possible age-associated differences in ECM protein structures which are tissue-region specific, but also highlights the ability of PLF as a proteomic tool to aid in biomarker discovery

Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics.

The current gold standard technique for SARS-CoV-2 diagnostics is hydrolysis probe-based RT-qPCR. Reliable testing requires reliable control reagents to monitor the efficiency of RNA extraction, reverse transcription and PCR amplification. Here we describe a custom RNA packaging system from the plant virus cowpea mosaic virus to produce virus-like particles that encapsidate specifically designed portions of the genome of SARS-CoV-2, the causative agent of COVID-19. These encapsidated mimics are highly stable particles which can be used either to spike patient swab samples for use as an in-tube extraction and reaction positive control in multiplex RT-qPCR, or alone as a side-by-side mock-positive control reagent. The selection of sequences in the packaged pseudogenomes ensures that these mimics are compatible with the most commonly used primer/probe combinations for SARS-CoV-2 diagnostics (including German Berlin Charité Hospital, American CDC, and Chinese CDC protocols). The plant transient expression system used to produce these encapsidated mimics is inherently low-cost, and sufficiently high-yielding that a single laboratory-scale preparation can provide enough positive control reagent for millions of tests

Wales: challenges and opportunities for post-Brexit environmental governance

Brexit represents a major change to environmental governance in Wales and the United Kingdom (UK), raising both opportunities and challenges. Welsh stakeholders are worried that English interests will predominate in the design of environmental governance after Brexit and are also concerned about the prospect of greater instability and weaker environmental protections. Crucially, the key planks of the UK government’s ‘Green Brexit’ strategy—the 25 Year Environment Plan (25 YEP) and Defra’s environmental governance and principles consultation—do not cover governance in the devolved nations. This gap in coverage raises the prospect of policy divergence and inconsistent implementation and enforcement across the UK. Most importantly, there is a strong fear in Wales that Welsh environmental policy ambition will be thwarted by Brexit and deregulatory pressure emanating from England

Wales: challenges and opportunities for post-Brexit environmental governance

Brexit represents a major change to environmental governance in Wales and the United Kingdom (UK), raising both opportunities and challenges. Welsh stakeholders are worried that English interests will predominate in the design of environmental governance after Brexit and are also concerned about the prospect of greater instability and weaker environmental protections. Crucially, the key planks of the UK government’s ‘Green Brexit’ strategy—the 25 Year Environment Plan (25 YEP) and Defra’s environmental governance and principles consultation—do not cover governance in the devolved nations. This gap in coverage raises the prospect of policy divergence and inconsistent implementation and enforcement across the UK. Most importantly, there is a strong fear in Wales that Welsh environmental policy ambition will be thwarted by Brexit and deregulatory pressure emanating from England

Quantitative analysis of chromatin interaction changes upon a 4.3 Mb deletion at mouse 4E2

BACKGROUND: Circular chromosome conformation capture (4C) has provided important insights into three dimensional (3D) genome organization and its critical impact on the regulation of gene expression. We developed a new quantitative framework based on polymer physics for the analysis of paired-end sequencing 4C (PE-4Cseq) data. We applied this strategy to the study of chromatin interaction changes upon a 4.3 Mb DNA deletion in mouse region 4E2. RESULTS: A significant number of differentially interacting regions (DIRs) and chromatin compaction changes were detected in the deletion chromosome compared to a wild-type (WT) control. Selected DIRs were validated by 3D DNA FISH experiments, demonstrating the robustness of our pipeline. Interestingly, significant overlaps of DIRs with CTCF/Smc1 binding sites and differentially expressed genes were observed. CONCLUSIONS: Altogether, our PE-4Cseq analysis pipeline provides a comprehensive characterization of DNA deletion effects on chromatin structure and function

Scotland: challenges and opportunities for post-Brexit environmental governance

Brexit represents a major change to environmental governance in Scotland and the United Kingdom (UK), raising both opportunities and challenges. It has prompted a constitutional dispute between the Scottish and UK governments, which may jeopardise future environmental governance. The current constitutional impasse has created even more uncertainty, making Brexit preparations highly challenging for government and civil society actors. Scottish stakeholders are worried that English interests will predominate in the design of environmental governance after Brexit and are also concerned about the prospect of greater instability and weaker environmental protections. Crucially, the key planks of the UK government’s ‘Green Brexit’ strategy—the 25 Year Environment Plan and the Department for Environment, Food and Rural Affairs’ (Defra) environmental governance and principles consultation—do not cover governance in the devolved nations. This gap in coverage raises the prospect of policy divergence and inconsistent implementation and enforcement across the UK. Most importantly, there is a strong fear in Scotland that Scottish environmental policy ambition will be thwarted by Brexit and deregulatory pressure from England

Northern Ireland: challenges and opportunities for post-Brexit environmental governance

Brexit represents a major change to environmental governance in Northern Ireland and the UK. Yet it is occurring at a time when Northern Ireland has no government, curtailing its ability to engage in both local and UK-wide preparations. Northern Irish stakeholders are worried that tensions between England and Scotland are dominating Brexit preparations, hampering discussions of UK-wide cooperation, as well as of the specific needs of Northern Ireland. They are concerned pre-existing environmental governance issues in the region (such as the lack of an independent environmental agency or the prevalence of cross-border environmental crime) will remain unaddressed, and that current North/South cooperation on environmental issues will be negatively impacted by the Brexit deal. Crucially, the key planks of the UK government’s ‘Green Brexit’ strategy (such as the commitments laid out in the 25 Year Environment Plan and the Environmental Principles and Governance consultation) do not cover the devolved nations. This raises the prospect of further policy divergence and inconsistent implementation and enforcement across the UK, and for Northern Ireland’s environment to continue deteriorating