The effects on grain quality traits of a grain serpin protein and the VPM 1 segment in southern Australian wheat breeding

Trabalho final do 6º ano médico com vista à atribuição do Grau de Mestre no âmbito do ciclo de estudos de mestrado integrado em medicina da Faculdade de Medicina de Coimbra.Introdução: Estudos prévios estabeleceram que até 12% dos doentes com enfarte agudo do miocárdio não demonstravam lesão coronária aparente ao exame angiográfico. Contudo, existe muito pouca informação disponível acerca deste fenómeno em enfartes agudos do miocárdio sem elevação do segmento ST. O objectivo deste estudo foi avaliar uma população de doentes com esse diagnóstico sujeitos a angiografia coronária, analisando e comparando as características clínicas e os prognósticos entre os que não revelaram lesão coronária aparente e os que apresentavam doença coronária epicárdica. Métodos: O nosso estudo avaliou 270 doentes com enfarte agudo do miocárdio sem elevação do segmento ST que foram sujeitos a angiografia coronária nas primeiras 72 horas de enfarte. Foram valorizadas as características demográficas, clínicas, parâmetros analíticos, achados electrocardiográficos, fracção de ejecção ventricular esquerda, duração do internamento e prognóstico.Previous studies established that up to 12% of patients with acute myocardial infarction did not present coronary atherosclerotic disease demonstrable with angiographic exam. However, little information is available about this phenomenon in acute non-STelevation myocardial infarction. We examined a population of patients with this diagnosis who had undergone coronary angiography, assessing and comparing the clinical characteristics and prognosis between the subset of patients with angiographically normal coronary arteries and the subset of patients with coronary disease

The comparative analyses of selected aspects of conservation and management of Vietnam’s national parks

The national parks in Vietnam are protected areas in the national system of special-use forests created to protect natural resources and biodiversity. In order to improve the effectiveness of management of national parks, the study assesses some current aspects of conservation and management of natural resources with respect to management plans, financial sources, staff, cooperative activities, causes of limited management capacity and threats to natural resources. Out of the total of 30 national parks, six are under the responsibility of the Vietnam Administration of Forestry (VNFOREST) and 24 national parks are managed by provincial authorities. It was found that most of the national parks have updated their management plans. Financial sources of funding for national parks mainly originated from the central and provincial budgets, with an average of 51% and 76% respectively. Fifty percent of national parks spent 40–60% of their total funding on conservation activities. About 85% of national parks’ staff had academic degrees, typically in the fields of forestry, agriculture and fisheries. Biodiversity conservation was considered a priority cooperative action in national parks with scientific institutes. Major causes of a limited management capacity of national parks included human population growth and pressure associated with resources use, lack of funding, limited human and institutional capacity and land use conflict/land grab. Illegal hunting, trapping, poaching and fishing, the illegal wildlife trade, illegal logging and firewood collecting appeared to be the most serious threats to the conservation and management of natural resources. In addition to these results, significant differences were found between the VNFOREST and provincial parks in terms of financial sources, staff and the threat of illegal logging and firewood collecting. The authors’ findings offer useful information for national park planners and managers, as well as policy makers and researchers in seeking solutions for the sustainable management of natural resources in national parks

First documentation of seismic stratigraphy and depositional signatures of Zhongsha atoll (Macclesfield Bank), South China Sea

Carbonate platforms form informative archives for paleoclimates and their internal structures can also hold crucial information about the tectonic history and carbonate evolution of the ocean basins. The Zhongsha atoll (Macclesfield Bank) forms the largest atoll system in the South China Sea with a surface area of 23500 km2. However, the internal structure and evolution of this atoll system is completely unknown. 2D multichannel seismic reflection data were acquired in 2017 over the Zhongsha atoll in the South China Sea to unravel the stratigraphy, geomorphology, depositional processes, and seismic facies of one of the world's largest atoll for the first time. This Neogene carbonate platform comprises more than 1 km thick carbonate sequence and overlies a metamorphic basement. The southeastern part of the atoll comprises a fault-controlled graben system, which was formed during the Cenozoic rifting stage of the South China Sea. Most of the faults trend NE-SW and E-W and terminate at or slightly above the top of Middle Miocene strata. Atolls and abundant organic reefs initiated on the positive relief and closely mimicked the underlying topography during the Early Miocene. Shallow-water carbonates continued growing through Middle Miocene to present times. Regional uplift led to subaerial exposure, termination of platform growth and karstification during the Miocene. We also reveal a number of fluid-flow features such as vertical sub-bottom venting features (chimneys and pipes), chaotic reflection zones, which provide the first evidence of active fluid venting in the area of Zhongsha atoll. The Neogene sedimentation history of Zhongsha atoll further provide an important paleoenvironmental context for future scientific drilling to better constrain the evolution of Asia Monsoon

New Insights into the Organization, Recombination, Expression and Functional Mechanism of Low Molecular Weight Glutenin Subunit Genes in Bread Wheat

The bread-making quality of wheat is strongly influenced by multiple low molecular weight glutenin subunit (LMW-GS) proteins expressed in the seeds. However, the organization, recombination and expression of LMW-GS genes and their functional mechanism in bread-making are not well understood. Here we report a systematic molecular analysis of LMW-GS genes located at the orthologous Glu-3 loci (Glu-A3, B3 and D3) of bread wheat using complementary approaches (genome wide characterization of gene members, expression profiling, proteomic analysis). Fourteen unique LMW-GS genes were identified for Xiaoyan 54 (with superior bread-making quality). Molecular mapping and recombination analyses revealed that the three Glu-3 loci of Xiaoyan 54 harbored dissimilar numbers of LMW-GS genes and covered different genetic distances. The number of expressed LMW-GS in the seeds was higher in Xiaoyan 54 than in Jing 411 (with relatively poor bread-making quality). This correlated with the finding of higher numbers of active LMW-GS genes at the A3 and D3 loci in Xiaoyan 54. Association analysis using recombinant inbred lines suggested that positive interactions, conferred by genetic combinations of the Glu-3 locus alleles with more numerous active LMW-GS genes, were generally important for the recombinant progenies to attain high Zeleny sedimentation value (ZSV), an important indicator of bread-making quality. A higher number of active LMW-GS genes tended to lead to a more elevated ZSV, although this tendency was influenced by genetic background. This work provides substantial new insights into the genomic organization and expression of LMW-GS genes, and molecular genetic evidence suggesting that these genes contribute quantitatively to bread-making quality in hexaploid wheat. Our analysis also indicates that selection for high numbers of active LMW-GS genes can be used for improvement of bread-making quality in wheat breeding

Copy Number Variation Affecting the Photoperiod-B1 and Vernalization-A1 Genes Is Associated with Altered Flowering Time in Wheat (Triticum aestivum)

The timing of flowering during the year is an important adaptive character affecting reproductive success in plants and is critical to crop yield. Flowering time has been extensively manipulated in crops such as wheat (Triticum aestivum L.) during domestication, and this enables them to grow productively in a wide range of environments. Several major genes controlling flowering time have been identified in wheat with mutant alleles having sequence changes such as insertions, deletions or point mutations. We investigated genetic variants in commercial varieties of wheat that regulate flowering by altering photoperiod response (Ppd-B1 alleles) or vernalization requirement (Vrn-A1 alleles) and for which no candidate mutation was found within the gene sequence. Genetic and genomic approaches showed that in both cases alleles conferring altered flowering time had an increased copy number of the gene and altered gene expression. Alleles with an increased copy number of Ppd-B1 confer an early flowering day neutral phenotype and have arisen independently at least twice. Plants with an increased copy number of Vrn-A1 have an increased requirement for vernalization so that longer periods of cold are required to potentiate flowering. The results suggest that copy number variation (CNV) plays a significant role in wheat adaptation

Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera Ceratopogonidae), using multi-locus DNA microsatellites

Bluetongue virus (BTV) is a major pathogen of ruminants that is transmitted by biting midges (Culicoides spp.). Australian BTV serotypes have origins in Asia and are distributed across the continent into two distinct episystems, one in the north and another in the east. Culicoides brevitarsis is the major vector of BTV in Australia and is distributed across the entire geographic range of the virus. Here, we describe the isolation and use of DNA microsatellites and gauge their ability to determine population genetic connectivity of C. brevitarsis within Australia and with countries to the north. Eleven DNA microsatellite markers were isolated using a novel genomic enrichment method and identified as useful for genetic analyses of sampled populations in Australia, northern Papua New Guinea (PNG) and Timor-Leste. Significant (P < 0.05) population genetic subdivision was observed between all paired regions, though the highest levels of genetic sub-division involved pair-wise tests with PNG (PNG vs. Australia (F-ST = 0.120) and PNG vs. Timor-Leste (F-ST = 0.095)). Analysis of multi-locus allelic distributions using STRUCTURE identified a most probable two-cluster population model, which separated PNG specimens from a cluster containing specimens from Timor-Leste and Australia. The source of incursions of this species in Australia is more likely to be Timor-Leste than PNG. Future incursions of BTV positive C. brevitarsis into Australia may be genetically identified to their source populations using these microsatellite loci. The vector's panmictic genetic structure within Australia cannot explain the differential geographic distribution of BTV serotypes

Association mapping of malting quality traits in UK spring and winter barley cultivar collections

Key Message: Historical malting quality data was collated from UK national and recommended list trial data and used in a GWAS. 25 QTL were identified, with the majority from spring barley cultivar sets. Abstract: In Europe, the most economically significant use of barley is the production of malt for use in the brewing and distilling industries. As such, selection for traits related to malting quality is of great commercial interest. In order to study the genetic basis of variation for malting quality traits in UK cultivars, a historical set of trial data was collated from national and recommended list trials from the period 1988 to 2016. This data was used to estimate variety means for 20 quality related traits in 451 spring barley cultivars, and 407 winter cultivars. Genotypes for these cultivars were generated using iSelect 9k and 50k genotyping platforms, and a genome wide association scan performed to identify malting quality quantitative trait loci (QTL). 24 QTL were identified in spring barley cultivars, and 2 from the winter set. A number of these correspond to known malting quality related genes but the remainder represents novel genetic variation that is accessible to breeders for the genetic improvement of new cultivars.Mark E. Looseley, Luke Ramsay, Hazel Bull, J. Stuart Swanston, Paul D. Shaw, Malcolm Macaulay, Allan Booth, Joanne R. Russell, Robbie Waugh, on behalf of the IMPROMALT Consortium, William T.B. Thoma