Spherical Vesicles Distorted by a Grafted Latex Bead: An Exact Solution

We present an exact solution to the problem of the global shape description of a spherical vesicle distorted by a grafted latex bead. This solution is derived by treating the nonlinearity in bending elasticity through the (topological) Bogomol'nyi decomposition technique and elastic compatibility. We recover the ``hat-model'' approximation in the limit of a small latex bead and find that the region antipodal to the grafted latex bead flattens. We also derive the appropriate shape equation using the variational principle and relevant constraints.Comment: 12 pages, 2 figures, LaTeX2e+REVTeX+AmSLaTe

Observation of an Efimov resonance in an ultracold mixture of atoms and weakly bound dimers

We discuss our recent observation of an atom-dimer Efimov resonance in an ultracold mixture of Cs atoms and Cs_2 Feshbach molecules [Nature Phys. 5, 227 (2009)]. We review our experimental procedure and present additional data involving a non-universal g-wave dimer state, to contrast our previous results on the universal s-wave dimer. We resolve a seeming discrepancy when quantitatively comparing our experimental findings with theoretical results from effective field theory.Comment: Conference Proceeding ICPEAC 2009 Kalamazoo, to appear in Journal of Physics: Conference Serie

Observation of an Efimov resonance in an ultracold mixture of atoms and weakly bound dimers

We discuss our recent observation of an atom-dimer Efimov resonance in an ultracold mixture of Cs atoms and Cs_2 Feshbach molecules [Nature Phys. 5, 227 (2009)]. We review our experimental procedure and present additional data involving a non-universal g-wave dimer state, to contrast our previous results on the universal s-wave dimer. We resolve a seeming discrepancy when quantitatively comparing our experimental findings with theoretical results from effective field theory.Comment: Conference Proceeding ICPEAC 2009 Kalamazoo, to appear in Journal of Physics: Conference Serie

Observation of an Efimov resonance in an ultracold mixture of atoms and weakly bound dimers

We discuss our recent observation of an atom-dimer Efimov resonance in an ultracold mixture of Cs atoms and Cs_2 Feshbach molecules [Nature Phys. 5, 227 (2009)]. We review our experimental procedure and present additional data involving a non-universal g-wave dimer state, to contrast our previous results on the universal s-wave dimer. We resolve a seeming discrepancy when quantitatively comparing our experimental findings with theoretical results from effective field theory.Comment: Conference Proceeding ICPEAC 2009 Kalamazoo, to appear in Journal of Physics: Conference Serie

Equilibrium onions?

We demonstrate the possibility of a stable equilibrium multi-lamellar ("onion") phase in pure lamellar systems (no excess solvent) due to a sufficiently negative Gaussian curvature modulus. The onion phase is stabilized by non-linear elastic moduli coupled to a polydisperse size distribution (Apollonian packing) to allow space-filling without appreciable elastic distortion. This model is compared to experiments on copolymer-decorated lamellar surfactant systems, with reasonable qualitative agreement

Entropic forces generated by grafted semiflexible polymers

The entropic force exerted by the Brownian fluctuations of a grafted semiflexible polymer upon a rigid smooth wall are calculated both analytically and by Monte Carlo simulations. Such forces are thought to play an important role for several cellular phenomena, in particular, the physics of actin-polymerization-driven cell motility and movement of bacteria like Listeria. In the stiff limit, where the persistence length of the polymer is larger than its contour length, we find that the entropic force shows scaling behavior. We identify the characteristic length scales and the explicit form of the scaling functions. In certain asymptotic regimes we give simple analytical expressions which describe the full results to a very high numerical accuracy. Depending on the constraints imposed on the transverse fluctuations of the filament there are characteristic differences in the functional form of the entropic forces; in a two-dimensional geometry the entropic force exhibits a marked peak.Comment: 21 pages, 18 figures, minor misprints correcte

Derivation of a Non-Local Interfacial Hamiltonian for Short-Ranged Wetting II: General Diagrammatic Structure

In our first paper, we showed how a non-local effective Hamiltionian for short-ranged wetting may be derived from an underlying Landau-Ginzburg-Wilson model. Here, we combine the Green's function method with standard perturbation theory to determine the general diagrammatic form of the binding potential functional beyond the double-parabola approximation for the Landau-Ginzburg-Wilson bulk potential. The main influence of cubic and quartic interactions is simply to alter the coefficients of the double parabola-like zig-zag diagrams and also to introduce curvature and tube-interaction corrections (also represented diagrammatically), which are of minor importance. Non-locality generates effective long-ranged many-body interfacial interactions due to the reflection of tube-like fluctuations from the wall. Alternative wall boundary conditions (with a surface field and enhancement) and the diagrammatic description of tricritical wetting are also discussed.Comment: (14 pages, 2 figures) Submitted J. Phys. Condens. Matte

Bi-defects of Nematic Surfactant Bilayers

We consider the effects of the coupling between the orientational order of the two monolayers in flat nematic bilayers. We show that the presence of a topological defect on one bilayer generates a nontrivial orientational texture on both monolayers. Therefore, one cannot consider isolated defects on one monolayer, but rather associated pairs of defects on either monolayer, which we call bi-defects. Bi-defects generally produce walls, such that the textures of the two monolayers are identical outside the walls, and different in their interior. We suggest some experimental conditions in which these structures could be observed.Comment: RevTeX, 4 pages, 3 figure

A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood.

The presence of tumour cells in the circulation may predict disease recurrence and metastasis. To improve on existing methods of cytological or immunocytological detection, we have developed a sensitive and quantitative technique for the detection of carcinoma cells in blood, using the reverse transcriptase polymerase chain reaction (RT-PCR) identifying transcripts of the pancarcinoma-associated tumour marker EGP-2 (KSA or 17-1A antigen). The amount of EGP2 mRNA was quantified using an internal recombinant competitor RNA standard with known concentration and which is both reversely transcribed and co-amplified in the same reaction, allowing for a reliable assessment of the initial amount of EGP2 mRNA in the sample. Calibration studies, seeding blood with MCF-7 breast carcinoma cells, showed that the assay can detect ten tumour cells among 1.0 x 10(6) leucocytes. The PCR assay revealed that normal bone marrow expresses low levels of EGP2 mRNA, although immunocytochemistry with the anti-EGP2 MAb MOC31 could not identify any positively stained cell. Analyses using this RT-PCR assay may prove to have applications to the assessment of circulating tumour cells in clinical samples

Dynamic Fluctuation Phenomena in Double Membrane Films

Dynamics of double membrane films is investigated in the long-wavelength limit including the overdamped squeezing mode. We demonstrate that thermal fluctuations essentially modify the character of the mode due to its nonlinear coupling to the transversal shear hydrodynamic mode. The corresponding Green function acquires as a function of the frequency a cut along the imaginary semi-axis. Fluctuations lead to increasing the attenuation of the squeezing mode it becomes larger than the `bare' value.Comment: 7 pages, Revte