4,618 research outputs found
Increased paclitaxel recovery from Taxus baccata vascular stem cells using novel in situ product recovery approaches
In this study, several approaches were tested to optimise the production and recovery of the widely used anticancer drug Taxol® (paclitaxel) from culturable vascular stem cells (VSCs) of Taxus baccata, which is currently used as a successful cell line for paclitaxel production. An in situ product recovery (ISPR) technique was employed, which involved combining three commercial macro-porous resin beads (HP-20, XAD7HP and HP-2MG) with batch and semi-continuous cultivations of the T. baccata VSCs after adding methyl jasmonate (Me-JA) as an elicitor. The optimal resin combination resulted in 234 ± 23 mg of paclitaxel per kg of fresh-weight cells, indicating a 13-fold improved yield compared to the control (with no resins) in batch cultivation. This resin treatment was further studied to evaluate the resins’ removal capacity of reactive oxygen species (ROS), which can cause poor cell growth or reduce product synthesis. It was observed that the ISPR cultivations had fourfold less intracellular ROS concentration than that of the control; thus, a reduced ROS concentration established by the resin contributed to increased paclitaxel yield, contrary to previous studies. These paclitaxel yields are the highest reported to date using VSCs, and this scalable production method could be applied for a diverse range of similar compounds utilising plant cell culture. Graphical Abstract: [Figure not available: see fulltext.]
The global oscillation network group site survey. II. Results
The Global Oscillation Network Group (GONG) Project will place a network of instruments around the world to observe solar oscillations as continuously as possible for three years. The Project has now chosen the six network sites based on analysis of survey data from fifteen sites around the world. The chosen sites are: Big Bear Solar Observatory, California; Mauna Loa Solar Observatory, Hawaii; Learmonth Solar Observatory, Australia; Udaipur Solar Observatory, India; Observatorio del Teide, Tenerife; and Cerro Tololo Interamerican Observatory, Chile.
Total solar intensity at each site yields information on local cloud cover, extinction coefficient, and transparency fluctuations. In addition, the performance of 192 reasonable components analysis. An accompanying paper describes the analysis methods in detail; here we present the results of both the network and individual site analyses.
The selected network has a duty cycle of 93.3%, in good agreement with numerical simulations. The power spectrum of the network observing window shows a first diurnal sidelobe height of 3 × 10⁻⁴ with respect to the central component, an improvement of a factor of 1300 over a single site. The background level of the network spectrum is lower by a factor of 50 compared to a single-site spectrum
A GHEP-ISFG collaborative study on the genetic variation of 38 autosomal indels for human identification in different continental populations
A collaborative effort was carried out by the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) to promote knowledge exchange between associate laboratories interested in the implementation of indel-based methodologies and build allele frequency databases of 38 indels for forensic applications. These databases include populations from different countries that are relevant for identification and kinship investigations undertaken by the participating laboratories. Before compiling population data, participants were asked to type the 38 indels in blind samples from annual GHEP-ISFG proficiency tests, using an amplification protocol previously described. Only laboratories that reported correct results contributed with population data to this study. A total of 5839 samples were genotyped from 45 different populations from Africa, America, East Asia, Europe and Middle East. Population differentiation analysis showed significant differences between most populations studied from Africa and America, as well as between two Asian populations from China and East Timor. Low FST values were detected among most European populations. Overall diversities and parameters of forensic efficiency were high in populations from all continents.RP is supported by a postdoctoral fellowship (SFRH/BPD/81986/2011) awarded by the
Portuguese Foundation for Science and Technology (FCT) and co-financed by the European
Social Fund (Human Potential Thematic Operational Programme – POPH
An Experimental Exploration of the QCD Phase Diagram: The Search for the Critical Point and the Onset of De-confinement
The QCD phase diagram lies at the heart of what the RHIC Physics Program is
all about. While RHIC has been operating very successfully at or close to its
maximum energy for almost a decade, it has become clear that this collider can
also be operated at lower energies down to 5 GeV without extensive upgrades. An
exploration of the full region of beam energies available at the RHIC facility
is imperative. The STAR detector, due to its large uniform acceptance and
excellent particle identification capabilities, is uniquely positioned to carry
out this program in depth and detail. The first exploratory beam energy scan
(BES) run at RHIC took place in 2010 (Run 10), since several STAR upgrades,
most importantly a full barrel Time of Flight detector, are now completed which
add new capabilities important for the interesting physics at BES energies. In
this document we discuss current proposed measurements, with estimations of the
accuracy of the measurements given an assumed event count at each beam energy.Comment: 59 pages, 78 figure
Charged and strange hadron elliptic flow in Cu+Cu collisions at = 62.4 and 200 GeV
We present the results of an elliptic flow analysis of Cu+Cu collisions
recorded with the STAR detector at 62.4 and 200GeV. Elliptic flow as a function
of transverse momentum is reported for different collision centralities for
charged hadrons and strangeness containing hadrons , ,
, in the midrapidity region . Significant reduction in
systematic uncertainty of the measurement due to non-flow effects has been
achieved by correlating particles at midrapidity, , with those at
forward rapidity, . We also present azimuthal correlations in
p+p collisions at 200 GeV to help estimating non-flow effects. To study the
system-size dependence of elliptic flow, we present a detailed comparison with
previously published results from Au+Au collisions at 200 GeV. We observe that
() of strange hadrons has similar scaling properties as were
first observed in Au+Au collisions, i.e.: (i) at low transverse momenta,
, scales with transverse kinetic energy, , and
(ii) at intermediate , , it scales with the number of
constituent quarks, . We have found that ideal hydrodynamic calculations
fail to reproduce the centrality dependence of () for
and . Eccentricity scaled values, , are larger
in more central collisions, suggesting stronger collective flow develops in
more central collisions. The comparison with Au+Au collisions which go further
in density shows depend on the system size, number of
participants . This indicates that the ideal hydrodynamic limit is
not reached in Cu+Cu collisions, presumably because the assumption of
thermalization is not attained.Comment: 18 pages, 14 figure
Inclusive pi^0, eta, and direct photon production at high transverse momentum in p+p and d+Au collisions at sqrt(s_NN) = 200 GeV
We report a measurement of high-p_T inclusive pi^0, eta, and direct photon
production in p+p and d+Au collisions at sqrt(s_NN) = 200 GeV at midrapidity (0
gamma gamma were detected in the
Barrel Electromagnetic Calorimeter of the STAR experiment at the Relativistic
Heavy Ion Collider. The eta -> gamma gamma decay was also observed and
constituted the first eta measurement by STAR. The first direct photon cross
section measurement by STAR is also presented, the signal was extracted
statistically by subtracting the pi^0, eta, and omega(782) decay background
from the inclusive photon distribution observed in the calorimeter. The
analysis is described in detail, and the results are found to be in good
agreement with earlier measurements and with next-to-leading order perturbative
QCD calculations.Comment: 28 pages, 30 figures, 6 tables, the updated version that was accepted
by Phys. Rev.
Growth of Long Range Forward-Backward Multiplicity Correlations with Centrality in Au+Au Collisions at = 200 GeV
Forward-backward multiplicity correlation strengths have been measured with
the STAR detector for Au+Au and collisions at =
200 GeV. Strong short and long range correlations (LRC) are seen in central
Au+Au collisions. The magnitude of these correlations decrease with decreasing
centrality until only short range correlations are observed in peripheral Au+Au
collisions. Both the Dual Parton Model (DPM) and the Color Glass Condensate
(CGC) predict the existence of the long range correlations. In the DPM the
fluctuation in the number of elementary (parton) inelastic collisions produces
the LRC. In the CGC longitudinal color flux tubes generate the LRC. The data is
in qualitative agreement with the predictions from the DPM and indicates the
presence of multiple parton interactions.Comment: 6 pages, 3 figures The abstract has been slightly modifie
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