159 research outputs found
Quantum Smoluchowski equation: Escape from a metastable state
We develop a quantum Smoluchowski equation in terms of a true probability
distribution function to describe quantum Brownian motion in configuration
space in large friction limit at arbitrary temperature and derive the rate of
barrier crossing and tunneling within an unified scheme. The present treatment
is independent of path integral formalism and is based on canonical
quantization procedure.Comment: 10 pages, To appear in the Proceedings of Statphys - Kolkata I
Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains
Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP–binding domains
Rational Design of a Plasmid Origin That Replicates Efficiently in Both Gram-Positive and Gram-Negative Bacteria
Background: Most plasmids replicate only within a particular genus or family
Functional Fluorescent Ca(2+) Indicator Proteins in Transgenic Mice under TET Control
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes—for the measurement of population activity, in particular—have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2
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