Changes on grape volatile composition after elicitors and nitrogen compounds foliar applications to 'Garnacha', 'Tempranillo' and 'Graciano' vines

Grape volatile compounds determine the wine quality and typicity. The aim of this work was to study the effects of several foliar applications in 'Garnacha', 'Tempranillo', and 'Graciano' grapevines in order to enhance their grape volatile composition. The field trial involved the application of two nitrogen compounds, urea (Ur) and phenylalanine (Phe), and two elicitors, methyl jasmonate (MeJ) and a yeast extract (YE). The analysis of grape volatile compounds was carried out by HS-SPME-GC-MS. For 'Garnacha', most terpenes, and C13 norisoprenoids increased their grape content by applying Ur and Phe, and especially MeJ. For 'Tempranillo', treatments with Ur and MeJ improved the synthesis of most terpenoids. For 'Graciano', a trend to decrease the terpenoids content in grapes with the treatments was observed; however, Phe application increased C13 norisoprenoids content. In conclusion, foliar application of, Phe or Ur, and an elicitor, MeJ, can be a useful tool to improve grape quality

Deshojado precoz en Viura y Malvasía riojana

En este trabajo se ha estudiado la aplicación de deshojado precoz en las variedades blancas Viura y Malvasía riojana como herramienta para el control del rendimiento y la mejora de la sanidad de la uva, con el objetivo de incrementar la calidad de sus vinos. Estas variedades presentan una producción elevada y racimos de gran tamaño, muy compactos, por lo que son bastante sensibles a Botrytis cinerea. Los resultados obtenidos indican que esta práctica puede plantearse como un método eficaz para el control del rendimiento y la mejora del estado sanitario de la uva; asimismo, favorece una correcta evolución del proceso de maduración, mejorando la composición de la uva debido al incremento del contenido de sólidos solubles y de compuestos fenólicos. La disminución de la acidez total y el aumento de pH que se producen en la uva, debido a la modificación del microclima de los racimos y al control del rendimiento, podría considerarse un inconveniente a tener en cuenta, sobre todo en el caso de variedades blancas que se caracterizan por un déficit de acidez total.Peer Reviewe

Gender bias in ecosystem restoration: from science to practice

The declaration of the United Nations Decade on Ecosystem Restoration 2020–2030 has established the need to focus on human rights in restoration initiatives, including gender equality. Although this goal raises a need to monitor gender biases on ecosystem restoration, we still lack basic gender information and evaluations on the current situation. The main purpose of this study is to analyze gender bias in ecosystem restoration covering three dimensions: research, outreach, and practice. We used scientific publications from the Restoration Ecology journal, mentions of these articles in Altmetric Explorer and Twitter, and projects from the Society for Ecological Restoration's database. First, we study gender bias among people leading ecosystem restoration initiatives in the three dimensions. Second, we assessed factors that could influence gender bias, including year, target ecosystem, and socioeconomic country development. Third, we analyzed whether the impact of scientific knowledge in society depends on the gender of the scientific team. Our results indicate that men were primary leaders in research, outreach, and practice initiatives in ecosystem restoration. There seems to be a trend over time toward equality in research, but gender inequality is still present in most types of ecosystems, with women leading more projects in more developed countries. The impact of scientific knowledge is independent of the author's gender, but research of male senior authors seems to reach society more easily. This broad perspective of inequality in the three dimensions can evolve toward gender equality, by applying gender approaches in restoration policies and initiatives. © 2022 Society for Ecological Restoration.Raw data is publicly available thanks to Web of Science, Altmetrics, Twitter and SER. Data and scripts used for the analysis are available via Figshare (Cruz‐Alonso et al. 2022 ). Funding: V.C.‐A.—Real Colegio Complutense postdoc fellowship; A.R.‐U.—Spanish State Research Agency through María de Maeztu Excellence Unit accreditation 2018–2022 (MDM‐2017‐0714); L.M.—Swiss National Science Foundation (PCEFP2_181115) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; L.M.‐B.—Ministerio de Ciencia e Innovación (PID2019‐106806GB‐I00) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; N.M.—predoctoral grant from Universidad de Alcalá; E.V.‐A.—European Commission (project SHOWCASE, H2020: 862480). We appreciate the support of the FIRE Foundation and the comments of M. Almaraz, M. Pajares, A. S. Moya, and D. Rohrer to improve the manuscript.Raw data is publicly available thanks to Web of Science, Altmetrics, Twitter and SER. Data and scripts used for the analysis are available via Figshare (Cruz‐Alonso et al. 2022 ). Funding: V.C.‐A.—Real Colegio Complutense postdoc fellowship; A.R.‐U.—Spanish State Research Agency through María de Maeztu Excellence Unit accreditation 2018–2022 (MDM‐2017‐0714); L.M.—Swiss National Science Foundation (PCEFP2_181115) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; L.M.‐B.—Ministerio de Ciencia e Innovación (PID2019‐106806GB‐I00) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; N.M.—predoctoral grant from Universidad de Alcalá; E.V.‐A.—European Commission (project SHOWCASE, H2020: 862480). We appreciate the support of the FIRE Foundation and the comments of M. Almaraz, M. Pajares, A. S. Moya, and D. Rohrer to improve the manuscript

Genetic variation and association analyses identify genes linked to fruit set-related traits in grapevine

Grapevine is one of the most valuable fruit crops in the world. Adverse environmental conditions reduce fruit quality and crop yield, so understanding the genetic and molecular mechanisms determining crop yield components is essential to optimize grape production. The analysis of a diverse collection of grapevine cultivars allowed us to evaluate the relationship between fruit set-related components of yield, including the incidence of reproductive disorders such as coulure and millerandage. The collection displayed a great phenotypic variation that we surveyed in a genetics association study using 15, 309 single nucleotide polymorphisms (SNPs) detected in the sequence of 289 candidate genes scattered across the 19 grapevine linkage groups. After correcting statistical models for population structure and linkage disequilibrium effects, 164 SNPs from 34 of these genes were found to associate with fruit set-related traits, supporting a complex polygenic determinism. Many of them were found in the sequence of different putative MADS-box transcription factors, a gene family related with plant reproductive development control. In addition, we observed an additive effect of some of the associated SNPs on the phenotype, suggesting that advantageous alleles from different loci could be pyramided to generate superior cultivars with optimized fruit production

Microbial volatile emissions promote accumulation of exceptionally high levels of starch in leaves in mono- and di-cotyledonous plants

Trabajo presentado en la X Reunión de Biología Molecular de plantas, celebrada en Valencia del 8 al 10 de julio de 2010.Peer Reviewe

Chromosome reorganizations in the origin of Tempranillo Blanco and other white grape cultivars

Póster presentado en la 11th International Conference on Grapevine Breeding and Genetics, celebrada en Pekín del 29 de julio al 2 de agosto de 2014.Acknowledgmcnts: Projects 8102011-26229 and INNOVINE.Peer Reviewe

Saturation of acyl chains converts cardiolipin from an antagonist to an activator of Toll-like receptor-4

Abstract: Cardiolipins (CLs) are tetra-acylated diphosphatidylglycerols found in bacteria, yeast, plants, and animals. In healthy mammals, CLs are unsaturated, whereas saturated CLs are found in blood cells from Barth syndrome patients and in some Gram-positive bacteria. Here, we show that unsaturated but not saturated CLs block LPS-induced NF-κB activation, TNF-α and IP-10 secretion in human and murine macrophages, as well as LPS-induced TNF-α and IL-1β release in human blood mononuclear cells. Using HEK293 cells transfected with Toll-like receptor 4 (TLR4) and its co-receptor Myeloid Differentiation 2 (MD2), we demonstrate that unsaturated CLs compete with LPS for binding TLR4/MD2 preventing its activation, whereas saturated CLs are TLR4/MD2 agonists. As a consequence, saturated CLs induce a pro-inflammatory response in macrophages characterized by TNF-α and IP-10 secretion, and activate the alternative NLRP3 inflammasome pathway in human blood-derived monocytes. Thus, we identify that double bonds discriminate between anti- and pro-inflammatory properties of tetra-acylated molecules, providing a rationale for the development of TLR4 activators and inhibitors for use as vaccine adjuvants or in the treatment of TLR4-related diseases. Graphical abstract

Material and instruments linked to the saharawi food: cultural, linguistic and semantic elements

En este artículo, centrado en la región denominada Sáhara Occidental (antiguo Sáhara Español), y directamente relacionado con la dialectología y las tradiciones populares, ofrecemos un estudio sobre el material y los instrumentos culinarios utilizados tradicionalmente (aunque mucho de ello se conserva hoy en día) por los habitantes autóctonos de dicha región. Para ello, abordamos cuestiones principales tales como el dialecto ḥassā-niya; la cocción y preparación tradicional de los alimentos en la zona objeto de estudio; los instrumentos y vasijas para el tratamiento de la leche; los instrumentos para el tratamiento del grano o el uso y aprovechamiento de arbustos para la obtención de la madera, ya que la inmensa mayoría de los recipientes de madera se fabricaban a partir de algunas de las especies leñosas más representativas del entorno. El enfoque es, básicamente, antropológico, con un evidente componente semántico, reflejado en el uso abundante de terminología árabe ḥassā-niya, con su equivalente castellano.This article focuses on the region called Western Sahara (former Spanish Sahara) and directly related to dialectology and folk traditions, we provide a study on the culinary equipment and instruments used once (although much of it remains today) by the indigenous inhabitants of the region. To this end, we address key issues such as Hassaniya dialect; cooking and food preparation in the traditional survey area; instruments and vessels for the treatment of milk; tools for treatment of grain or the use and utilization of shrubs for obtaining wood, since the vast majority of wooden containers were made of some of the most representative woody species in the area. The approach is basically anthropological, with obvious semantic component, reflected in the generous use of Arab-Hassaniya terminology, with its equivalent in Spanish

Enteritis secundaria a nivolumab, una causa creciente de diarrea

La inmunoterapia es una herramienta cada vez más utilizada en el campo de la oncología. Conviene conocerla debido a sus crecientes usos, entre los que se incluye el tratamiento de tumores del aparato digestivo (hepatocarcinoma1, adenocarcinoma colorrectal con alta inestabilidad en microsatelites2) así como por las reacciones adversas que con elevada frecuencia afectan al tubo digestivo. Presentamos el caso de un varón de 74 años, con antecedentes personales de enfermedad pulmonar obstructiva crónica (EPOC) y melanoma con metástasis pulmonares. Debido a estas patologías tomaba de manera habitual inhaladores de salbutamol y había estado en tratamiento con nivolumab, suspendido hacía cuatro meses tras conseguir una respuesta radiológica completa de las metástasis pulmonares. El paciente refería cuadro diarreico de un mes de evolución, consistente en tres a cuadro deposiciones (Bristol 5-6) sin productos patológicos, que afectaban el descanso nocturno, asociaban molestias centroabdominales intermitentes y pérdida de peso de unos 3-4 kg. No había ingerido alimentos crudos, antibióticos o nuevas medicaciones. Tampoco había convivientes con la misma sintomatología ni había realizado viajes al extranjero. Negaba cualquier otra sintomatología y antecedentes familiares de interés. La exploración física era anodina a excepción de unas ligeras molestias a la palpación profunda en mesogastrio..

Aproximación al manejo de la disección del tronco celíaco

El dolor abdominal constituye uno de los motivos de consulta más frecuentes en los servicios de Urgencias y de Aparato Digestivo. Además, el diagnóstico diferencial supone un importante reto, dado el amplio abanico de entidades clínicas que pueden provocarlo, algunas de ellas con un pronóstico desfavorable. En este sentido, en algunas cohortes no se ha llegado a un diagnóstico específico en más de un 30% de los casos1, 2. Se presenta el caso de un varón de 40 años, fumador activo desde hace más de 20 años, sin otros antecedentes personales ni familiares de interés, excepto traumatismo cerrado abdominal hace cuatro años, que no seguía tratamiento farmacológico habitual. Presentaba dolor abdominal epigástrico continuo e irradiado hacia ambos hipocondrios, de ocho horas de evolución. No asociaba ictericia mucocutánea ni coluria o acolia, tampoco náuseas ni vómitos ni alteraciones en el ritmo y características de las deposiciones. No presentaba fiebre, síndrome constitucional ni otra sintomatología asociada. A la exploración física destacaba dolor a la palpación de epigastrio, sin signos de irritación peritoneal, con peristaltismo y pulsos distales conservados. Asimismo, no presentaba signos de colagenopatía. Para una primera aproximación diagnóstica se realizó analítica de sangre, destacando como únicos hallazgos ligera alteración del perfil hepático (AST 66 U/L, ALT 58 U/L, GGT 106 U/L, FA 76 U/L) con amilasemia, ..