387 research outputs found
Kalman-filter control schemes for fringe tracking. Development and application to VLTI/GRAVITY
The implementation of fringe tracking for optical interferometers is
inevitable when optimal exploitation of the instrumental capacities is desired.
Fringe tracking allows continuous fringe observation, considerably increasing
the sensitivity of the interferometric system. In addition to the correction of
atmospheric path-length differences, a decent control algorithm should correct
for disturbances introduced by instrumental vibrations, and deal with other
errors propagating in the optical trains. We attempt to construct control
schemes based on Kalman filters. Kalman filtering is an optimal data processing
algorithm for tracking and correcting a system on which observations are
performed. As a direct application, control schemes are designed for GRAVITY, a
future four-telescope near-infrared beam combiner for the Very Large Telescope
Interferometer (VLTI). We base our study on recent work in adaptive-optics
control. The technique is to describe perturbations of fringe phases in terms
of an a priori model. The model allows us to optimize the tracking of fringes,
in that it is adapted to the prevailing perturbations. Since the model is of a
parametric nature, a parameter identification needs to be included. Different
possibilities exist to generalize to the four-telescope fringe tracking that is
useful for GRAVITY. On the basis of a two-telescope Kalman-filtering control
algorithm, a set of two properly working control algorithms for four-telescope
fringe tracking is constructed. The control schemes are designed to take into
account flux problems and low-signal baselines. First simulations of the
fringe-tracking process indicate that the defined schemes meet the requirements
for GRAVITY and allow us to distinguish in performance. In a future paper, we
will compare the performances of classical fringe tracking to our Kalman-filter
control.Comment: 17 pages, 8 figures, accepted for publication in A&
Antitumour and antiangiogenic effects of Aplidin® in the 5TMM syngeneic models of multiple myeloma
Aplidin® is an antitumour drug, currently undergoing phase II evaluation in different haematological and solid tumours. In this study, we analysed the antimyeloma effects of Aplidin in the syngeneic 5T33MM model, which is representable for the human disease. In vitro, Aplidin inhibited 5T33MMvv DNA synthesis with an IC50 of 3.87 nM. On cell-cycle progression, the drug induced an arrest in transition from G0/G1 to S phase, while Western blot showed a decreased cyclin D1 and CDK4 expression. Furthermore, Aplidin induced apoptosis by lowering the mitochondrial membrane potential, by inducing cytochrome c release and by activating caspase-9 and caspase-3. For the in vivo experiment, 5T33MM-injected C57Bl/KaLwRij mice were intraperitoneally treated with vehicle or Aplidin (90 μg kg−1 daily). Chronic treatment with Aplidin was well tolerated and reduced serum paraprotein concentration by 42% (P<0.001), while BM invasion with myeloma cells was decreased by 35% (P<0.001). Aplidin also reduced the myeloma-associated angiogenesis to basal values. This antiangiogenic effect was confirmed in vitro and explained by inhibition of endothelial cell proliferation and vessel formation. These data indicate that Aplidin is well tolerated in vivo and its antitumour and antiangiogenic effects support the use of the drug in multiple myeloma
Influence of Vectors' Risk-Spreading Strategies and Environmental Stochasticity on the Epidemiology and Evolution of Vector-Borne Diseases: The Example of Chagas' Disease
Insects are known to display strategies that spread the risk of encountering unfavorable conditions, thereby decreasing the extinction probability of genetic lineages in unpredictable environments. To what extent these strategies influence the epidemiology and evolution of vector-borne diseases in stochastic environments is largely unknown. In triatomines, the vectors of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease, juvenile development time varies between individuals and such variation most likely decreases the extinction risk of vector populations in stochastic environments. We developed a simplified multi-stage vector-borne SI epidemiological model to investigate how vector risk-spreading strategies and environmental stochasticity influence the prevalence and evolution of a parasite. This model is based on available knowledge on triatomine biodemography, but its conceptual outcomes apply, to a certain extent, to other vector-borne diseases. Model comparisons between deterministic and stochastic settings led to the conclusion that environmental stochasticity, vector risk-spreading strategies (in particular an increase in the length and variability of development time) and their interaction have drastic consequences on vector population dynamics, disease prevalence, and the relative short-term evolution of parasite virulence. Our work shows that stochastic environments and associated risk-spreading strategies can increase the prevalence of vector-borne diseases and favor the invasion of more virulent parasite strains on relatively short evolutionary timescales. This study raises new questions and challenges in a context of increasingly unpredictable environmental variations as a result of global climate change and human interventions such as habitat destruction or vector control.Centro de Estudios Parasitológicos y de Vectore
Influence of Vectors' Risk-Spreading Strategies and Environmental Stochasticity on the Epidemiology and Evolution of Vector-Borne Diseases: The Example of Chagas' Disease
Insects are known to display strategies that spread the risk of encountering unfavorable conditions, thereby decreasing the extinction probability of genetic lineages in unpredictable environments. To what extent these strategies influence the epidemiology and evolution of vector-borne diseases in stochastic environments is largely unknown. In triatomines, the vectors of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease, juvenile development time varies between individuals and such variation most likely decreases the extinction risk of vector populations in stochastic environments. We developed a simplified multi-stage vector-borne SI epidemiological model to investigate how vector risk-spreading strategies and environmental stochasticity influence the prevalence and evolution of a parasite. This model is based on available knowledge on triatomine biodemography, but its conceptual outcomes apply, to a certain extent, to other vector-borne diseases. Model comparisons between deterministic and stochastic settings led to the conclusion that environmental stochasticity, vector risk-spreading strategies (in particular an increase in the length and variability of development time) and their interaction have drastic consequences on vector population dynamics, disease prevalence, and the relative short-term evolution of parasite virulence. Our work shows that stochastic environments and associated risk-spreading strategies can increase the prevalence of vector-borne diseases and favor the invasion of more virulent parasite strains on relatively short evolutionary timescales. This study raises new questions and challenges in a context of increasingly unpredictable environmental variations as a result of global climate change and human interventions such as habitat destruction or vector control.Centro de Estudios Parasitológicos y de Vectore
Assimilable Organic Carbon (AOC) in Soil Water Extracts Using Vibrio harveyi BB721 and Its Implication for Microbial Biomass
Assimilable organic carbon (AOC) is commonly used to measure the growth potential of microorganisms in water, but has not yet been investigated for measuring microbial growth potential in soils. In this study, a simple, rapid, and non-growth based assay to determine AOC in soil was developed using a naturally occurring luminous strain Vibrio harveyi BB721 to determine the fraction of low molecular weight organic carbon in soil water extract. Calibration of the assay was achieved by measuring the luminescence intensity of starved V. harveyi BB721 cells in the late exponential phase with a concentration range from 0 to 800 µg l−1 glucose (equivalent to 0–16.0 mg glucose C kg−1 soil) with the detection limit of 10 µg l−1 equivalent to 0.20 mg glucose C kg−1 soil. Results showed that bioluminescence was proportional to the concentration of glucose added to soil. The luminescence intensity of the cells was highly pH dependent and the optimal pH was about 7.0. The average AOC concentration in 32 soils tested was 2.9±2.2 mg glucose C kg−1. Our data showed that AOC levels in soil water extracts were significantly correlated (P<0.05) with microbial biomass determined as microbial biomass carbon, indicating that the AOC concentrations determined by the method developed might be a good indicator of soil microbial biomass. Our findings provide a new approach that may be used to determine AOC in environmental samples using a non-growth bioluminescence based assay. Understanding the levels of AOC in soil water extract provides new insights into our ability to estimate the most available carbon pool to bacteria in soil that may be easily assimilated into cells for many metabolic processes and suggest possible the links between AOC, microbial regrowth potential, and microbial biomass in soils
The orbits of subdwarf B + main-sequence binaries. I: The sdB+G0 system PG 1104+243
The predicted orbital period histogram of an sdB population is bimodal with a
peak at short ( 250 days) periods. Observationally, there
are many short-period sdB systems known, but only very few long-period sdB
binaries are identified. As these predictions are based on poorly understood
binary interaction processes, it is of prime importance to confront the
predictions to observational data. In this contribution we aim to determine the
absolute dimensions of the long-period sdB+MS binary system PG1104+243.
High-resolution spectroscopy time-series were obtained with HERMES at the
Mercator telescope at La Palma, and analyzed to obtain radial velocities of
both components. Photometry from the literature was used to construct the
spectral energy distribution (SED) of the binary. Atmosphere models were used
to fit this SED and determine the surface gravity and temperature of both
components. The gravitational redshift provided an independent confirmation of
the surface gravity of the sdB component. An orbital period of 753 +- 3 d and a
mass ratio of q = 0.637 +- 0.015 were found from the RV-curves. The sdB
component has an effective temperature of Teff = 33500 +- 1200 K and a surface
gravity of logg = 5.84 +- 0.08 dex, while the cool companion is found to be a
G-type star with Teff = 5930 +- 160 K and logg = 4.29 +- 0.05 dex. Assuming a
canonical mass of Msdb = 0.47 Msun, the MS component has a mass of 0.74 +- 0.07
Msun, and its Teff corresponds to what is expected for a terminal age
main-sequence star with sub-solar metalicity. PG1104+243 is the first
long-period sdB binary in which accurate physical parameters of both components
could be determined, and the first sdB binary in which the gravitational
redshift is measured. Furthermore, PG1104+243 is the first sdB+MS system that
shows consistent evidence for being formed through stable Roche-lobe overflow.Comment: Accepted by A&A on 05-10-201
X-ray and Infrared Microanalyses of <i>Mona Lisa</i>'s Ground Layer and Significance Regarding Leonardo da Vinci's Palette
An exceptional microsample from the ground layer of Leonardo da Vinci's Mona Lisa was analyzed by high-angular resolution synchrotron X-ray diffraction and micro Fourier transform infrared spectroscopy, revealing a singular mixture of strongly saponified oil with high lead content and a cerussite (PbCO3)-depleted lead white pigment. The most remarkable signature in the sample is the presence of plumbonacrite (Pb5(CO3)3O(OH)2), a rare compound that is stable only in an alkaline environment. Leonardo probably endeavored to prepare a thick paint suitable for covering the wooden panel of the Mona Lisa by treating the oil with a high load of lead II oxide, PbO. The review of Leonardo's manuscripts (original and latter translation) to track the mention of PbO gives ambiguous information. Conversely, the analysis of fragments from the Last Supper confirms that not only PbO was part of Leonardo's palette, through the detection of both litharge (α-PbO) and massicot (β-PbO) but also plumbonacrite and shannonite (Pb2OCO3), the latter phase being detected for the first time in a historical painting.</p
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Discovery of Unusual Minerals in Paleolithic Black Pigments from Lascaux (France) and Ekain (Spain)
Analyses of archaeological materials aim to rediscover the know-how of Prehistoric people by determining the nature of the painting matter, its preparation mode, and the geographic origin of its raw materials. This study deals with identification of manganese oxides in black pigments by micro-XANES (X-ray absorption near-edge structure) based on previous TEM (transmission electron microscopy) studies. Complex mixtures of the manganese oxides studied are present in some of mankind's oldest known paintings, namely those from the caves of Lascaux (Dordogne, France) and Ekain (Basque country, Spain). Scarce manganese oxide minerals, including groutite, hausmannite, and manganite, were found for the first time in Paleolithic art at these archaeological sites. Because there are no known deposits of such minerals in these areas, more distant origins and trade routes are inferred. The closest known Mn-rich geological province for Lascaux is the central Pyrenees, which is {approx} 250 km from the Dordogne area
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