НОВІТНІ ПІДХОДИ ДО КОРЕКЦІЇ БОЛЬОВОГО СИНДРОМУ У ХВОРИХ З ОБЛІТЕРУЮЧИМ АТЕРОСКЛЕРОЗОМ НИЖНІХ КІНЦІВОК, ЩО ПРООПЕРОВАНІ МЕТОДОМ ЕНДОВАСКУЛЯРНОЇ БАЛОНОПЛАСТИКИ

Purpose. In order to optimize the intraoperative anesthesia tactics we used epidural anesthesia.Materials and methods. There were 40 patients. They were randomized into 2 groups of 20 people. Patients of I group performed analgosedation, group – II epidural analgesia.Results. The use of epidural anesthesia sol. 0.125% bupivacaine in the volume of 30 ml with the addition of 0.12 mg of sol. Buprenorphine provided adequate levels of both intra- and postoperative analgesia of balonplastics of obliterating atherosclerosis of the lower limbs.З метою оптимізувати інтраопераційну анестезіологічну тактику, для знеболювання було використано епідуральну аналгезію.Матеріали і методи. 40 пацієнтів були рандомізовано розподілені на дві групи по 20 осіб. Хворим І групи проводилась аналгоседація, ІІ групи – епідуральна анестезія.Результати. Застосування епідуральної анестезії розчином 0,125% бупівакаїну в об’ємі 30 мл з додавання 0,12 мг розчину бупренорфіну забезпечувало адекватний рівень як інтра-, так і післяопераційної аналгезії при балонопластиці облітеруючого атеросклерозу нижніх кінцівок

Syntaxonomy and ecological differentiation of the pioneer vegetation of Ukraine. 2. Helichryso-Crucianelletea maritimae, Festucetea vaginatae, Koelerio-Corynephoretea canescentis classes

Pioneer psammophytic vegetation is usually developed on wind-drift sandy substrates such as arenas, spits, beaches, river terraces, and this vegetation occupies significant areas in all three natural zones of Ukraine. The Koelerio-Corynephoretea canescentis class was represented by 13 associations, 3 alliances and 1 order; Festucetea vaginatae class by 22 associations, 2 alliances and 1 order; Helichryso-Crucianelletea maritimae by 10 associations, 4 alliances and 1 order. The results of cluster analysis and synoptic tables of the classes are presented. 9 alliances are briefly described. Leading factors of territorial and ecological differentiation are identified. It was found that the territorial distribution of plant communities is influenced by the character of ecotope mesorelief, soil composition and humus horizon thickness, as well as the degree of eolian processes development. The main factors of their ecological differentiation are soil acidity, salt regime and ombroregime. Based on the results of DCA-ordination of syntaxa within certain vegetation classes, it was found that their distribution is influenced by factors that correlate with the environment-specific conditions. It has emerged that an ecological differentiation of syntaxa within Festucetea vaginatae is determined by the integrated effect of gradients, and soil salinity is leading among them. Temperature regime and climate continentality are leading factors in the distribution of syntaxa within the Koelerio-Corynephoretea canescentis class. The gradients of ombroregime and soil humidity have a significant impact. The distribution of communities of the Helichryso-Crucianelletea maritimae class in the ecological space is determined mainly by factors of variability of damping, ombroregime and climate continentality. The author’s syntaxonomic concept assumes the independence of the studied classes: Koelerio-Corynephoretea canescentis, Helichryso-Crucianelletea maritimae and Festucetea vaginatae, conside­ring that the leading factors of community differentiation of the Festucetea vaginatae and Koelerio-Corynephoretea canescentis classes are the origin (genesis) of sandy substrates, as well as soil acidity. Phytosociological analysis of a large number of relevés of coastal littoral vegetation also provides support for independence of the Helichryso-Crucianelletea maritimae and Ammophiletea classes diffe­rent floristically and ecologically. A review of the psammophytic vegetation of Ukraine will determine the place of the selected syntaxonomic units in the pan-European system

Psychological and Pedagogical Aspects of the Development of Integrative Readiness of Future Specialists for Professional Activity

The need to develop an integrative readiness of future specialists is a relevant scientific problem. The reasons for this could be based on the fact that that the specialists-to-be were expected to be involved in the fierce competition for vacancies and areas of activity, have modern information and communication tools, i.e. have an integrative readiness for professional activity. Institutions do not, however, have a single integration (interdisciplinary) framework for training, do not provide a comprehensive educational information, technical tools, strategies and technologies of education, reasoned psychological and pedagogical conditions. According to the author, the integrative readiness of future specialists for professional activity is a system-personality formation that reflects the unity of theoretical and managerial training and practical ability of students to comprehensively apply regulatory, socio-economic, psychological and pedagogical methods and technologies for solving different problems. This readiness reflects the unity of the motivational inclination of future specialists to professional activity and knowledge of practical technologies for solving a wide range of professional problems in personal and business interactions. The research methodology is based on the concept of key competence, which provides systematization, classification of significant problems, development of a matrix of significant problems, and determination of overall strategy, management technology of professional training development process. Students and teachers can use research materials can be used by in educational and practical activities; developers of content, organizational forms and methods of professional training to improve the practical component of curricula and standards of their development

Rare steppe plant communities in Ukraine: Status, threats and their minimization

Nowadays, the impact of anthropogenic activities on natural vegetation is constantly increasing, the level of threats is raised, and newer risk factors are emerging. Recent trends in the anthropogenic impact on plant communities are extremely pronounced, especially on those listed in the Green Book of Ukraine (GBU). Identifying such trends is required for the further development of strategic and tactical planning for the preservation and restoration of rare grass, shrub, and subshrub steppe, petrophyte and psammophyte plant communities of the steppe and forest-steppe zones of Ukraine. In addition to well-established threat factors that cause changes in the habitat of plant communities or mechanically affect plants, new specific threats occur. Today, the most important among them in Ukraine are as follows: climate change, military activity, growing population in the industrial cities, population poverty and government corruption, changes in forms of land ownership and the creation of a land market, lack of knowledge and effective policy, including lack of popular scientific information about the status of specific species and plant groupings, inadequate management of protected areas, uncoordinated environmental protection measures, ineffective sanctions, insufficient monitoring of the consumption of natural biological resources. The preservation of rare grass, shrubby and subshrubby communities in the steppe zone of Ukraine should be provided with proper support at the state level. There is a pressing need for a law of Ukraine “On the preservation of the steppes in Ukraine” and this will require mechanisms for its implementation. The issue of developing a strategy for the conservation and balanced use of steppe ecosystems in Ukraine, whose area is one of the largest in Europe, has long been raised. The main goal of the strategy is the actual preservation of steppe communities (most of which are currently rare) ensuring their restoration, minimizing degradation, and stopping biodiversity loss. In order to develop specific actions to eliminate threats or reduce their impact on rare plant groupings, it is required to investigate the causes of threats and assess their level and duration. This is required to preserve the landscape and biotic diversity in the steppe zone of Ukraine

Прогностичні фактори тривалості перебування у стаціонарі пацієнтів із гострим коронарним синдромом після екстреної ендоваскулярної реваскуляризації міокарда

Мета. Визначити ступінь впливу незалежних один від одного передопераційних чинників на єдиний залежний чинник - тривалість госпіталізації пацієнтів із гострим коронарним синдромом після екстреної ендоваскулярної реваскуляризації міокарда. Матеріали і методи. Зібрано та статистично опрацьовано дані історій хвороб 40 пацієнтів віком до 60 років (1-ша група) та 40 пацієнтів віком 60 років і старше (2-га група). Методом статистичного опрацювання даних обрано кореляційно-регресійний аналіз. Результати. У пацієнтів 1-ї групи на тривалість госпіталізації впливали (в порядку зменшення значущості): передопераційні показники гемоглобіну та гематокриту, рівні тропоніну І та загального холестерину, ступінь оклюзії коронарних артерій, індекс маси тіла, фракція викиду лівого шлуночка та об’єм післяопераційної крововтрати. У пацієнтів 2-ї групи єдиним фактором, що істотно вплинув на тривалість госпіталізації, був вік. Висновки. Предикторами тривалості госпіталізації у віковій групі до 60 років слугують майже всі включені до дослідження незалежні фактори, тоді як у віковій групі 60 років і старше стійка кореляція характерна лише для фактора віку

Evidence That a Respiratory Shield in \u3cem\u3eEscherichia coli\u3c/em\u3e Protects a Low-Molecular-Mass Fe-\u3csup\u3eII\u3c/sup\u3e Pool from O\u3csub\u3e2\u3c/sub\u3e-Dependent Oxidation

Iron is critical for virtually all organisms, yet major questions remain regarding the systems\u27 level understanding of iron in whole cells. Here, we obtained Mössbauer and EPR spectra of Escherichia coli cells prepared under different nutrient iron concentrations, carbon sources, growth phases, and O2 concentrations to better understand their global iron content. We investigated wild-type cells and those lacking Fur, FtnA, Bfr, and Dps proteins. The coarse-grain iron content of exponentially growing cells consisted of iron-sulfur clusters, variable amounts of nonheme high-spin FeII species, and an unassigned residual quadrupole doublet. The iron in stationary-phase cells was dominated by magnetically-ordered FeIII ions due to oxyhydroxide nanoparticles. Analysis of cytosolic extracts by size-exclusion chromatography detected by an online inductively-coupled plasma mass spectrometer revealed a low-molecular-mass (4LMM) FeII pool consisting of two iron complexes with masses of ~ 500 (major) and ~1300 (minor) Da. They appeared to be high-spin FeII species with mostly O donor ligands, perhaps a few N donors, and probably no S donor. Surprisingly, the iron content of E. coli and its reactivity with O2 were remarkably similar to those of mitochondria. In both cases, a respiratory shield composed of membrane-bound iron-rich respiratory complexes may protect the LMM FeII pool from reacting with O2. When exponentially growing cells transition to stationary phase, the shield deactivates as metabolic activity declines. Given the universality of oxidative phosphorylation in aerobic biology, the iron content and respiratory shield in other aerobic prokaryotes might be similar to those of E. coli and mitochondria

Features of ecological differentiation of halophytic, steppe and petrophytic vegetation in the valley of the Liman Kuyalnik (Odesa Oblast)

Assessment of key environmental factors that influence vegetation distribution and formation of plant communities is one of the most important challenges in modern phytocenology. Nowadays, several bioindication systems are applied to determine ecological specificity of plant communities and to establish the leading factors for their environmental differentiation. The system most widely used in Europe, that of H. Ellenberg, contains a numerical score on 6 ecological factors. On the example of vegetation of the valley of the Liman Kuyalnik, Y. Didukh developed the synphytoindication method based on evaluation of phytocenoses with respect to 12 ecological factors: 7 edaphic factors and 5 climatic factors; the method determines a more accurate and complete presentation of the analysis. In the valley of the Liman Kuyalnik (Odesa Oblast) the largest area is covered with halophytic and steppe vegetation. Halophytic vegetation (Therosalicornietea, Festuco-Puccinellietea classes, Juncetea maritimi, Bolboschoenetea maritimi) predominated in the shoreline areas of the valley, whereas steppe (Festuco-Brometea) and petrophytic (Sedo-Scleranthetea) vegetation dominated on the slope sites. With the application of DCA-ordination and synphytoindication methods it was established that distribution of plant communities in the hyper-space of the environmental conditions was most strongly correlated with edaphic factors, whereas microclimatic (light intensity) and climatic (thermo-regime) conditions had somewhat less influence on their differentiation. Water regime and level of soil salinity served as key factors for syntaxa of halophytic vegetation; moisture variability and salt regime, as well as soil moisture and carbonate content were key factors for the steppe vegetation, and thermo-regime was the main factor for petrophytic-steppe and petrophytic vegetation. The "eco-spaces" of these groups largely overlap. Halophytic cenoses are characterized by quite wide ecological ranges by most ecological factors. Steppe communities show much less ecological diversity. In the valley of the liman, all the steppe communities were characterized by stenotopicity in relation to most ecological factors; these factors complexly determine the specificity and diversity of biotopes within the valley, which are unique and require protection and the taking of appropriate measures, depending on the changes in activity of one or another limiting factor. Nowadays, the valley of the Liman Kuyalnik is in a state of environmental disaster. The established relationships in ecological differentiation of plant communities will be applied to further monitoring of biodiversity state, preservation and possible restoration of vegetation types that were native for this unique territory

Substantiation of thermomechanical technology parameters of absorbing levels isolation of the boreholes

The aim of the work is to improve the thermomechanical absorption insulation technology horizons of drilling wells by the established regularities of change and the substantiation of its regime parameters from the composition and physical-mechanical properties strengthen thermoplastic composite material and, on this basis, development a technological regulation containing recommendations on the manufacture of composites and organizations laying work, designing and isolation of the absorption zones of the washing liquid in the drilling rigs wells. The tasks set were solved by complex method research that contains analysis and synthesis of literary and patent sources, conducting analytical, experimental and industrial research. Experimental processing data was carried out using methods of mathematical statistics. Experimental research is carried out using the provisions of the theory of scientific experiment and theory random processes. The evaluation of the effectiveness of the results was carried out in production conditions

Large-scale mapping of bioactive peptides in structural and sequence space

Health-enhancing potential bioactive peptide (BP) has driven an interest in food proteins as well as in the development of predictive methods. Research in this area has been especially active to use them as components in functional foods. Apparently, BPs do not have a given biological function in the containing proteins and they do not evolve under independent evolutionary constraints. In this work we performed a large-scale mapping of BPs in sequence and structural space. Using well curated BP deposited in BIOPEP database, we searched for exact matches in non-redundant sequences databases. Proteins containing BPs, were used in fold-recognition methods to predict the corresponding folds and BPs occurrences were mapped. We found that fold distribution of BP occurrences possibly reflects sequence relative abundance in databases. However, we also found that proteins with 5 or more than 5 BP in their sequences correspond to well populated protein folds, called superfolds. Also, we found that in well populated superfamilies, BPs tend to adopt similar locations in the protein fold, suggesting the existence of hotspots. We think that our results could contribute to the development of new bioinformatics pipeline to improve BP detection.Fil: Nardo, Agustina Estefania. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Añon, Maria Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Parisi, Gustavo Daniel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

A multi-pronged approach targeting SARS-CoV-2 proteins using ultra-large virtual screening.

The unparalleled global effort to combat the continuing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic over the last year has resulted in promising prophylactic measures. However, a need still exists for cheap, effective therapeutics, and targeting multiple points in the viral life cycle could help tackle the current, as well as future, coronaviruses. Here, we leverage our recently developed, ultra-large-scale in silico screening platform, VirtualFlow, to search for inhibitors that target SARS-CoV-2. In this unprecedented structure-based virtual campaign, we screened roughly 1 billion molecules against each of 40 different target sites on 17 different potential viral and host targets. In addition to targeting the active sites of viral enzymes, we also targeted critical auxiliary sites such as functionally important protein-protein interactions