Modelling full waveform Lidar data on forest structures at plot level : a sensitivity analysis of forest and sensor main characteristics on full-waveform simulated data

[Departement_IRSTEA]Territoires [TR1_IRSTEA]SYNERGIE [Axe_IRSTEA]TETIS-ATTOSSilviLaser, La Grande Motte, FRA, 28-/09/2015 - 30/09/2015International audienceA new approach for LIDAR altimetry mission for biomass applications (tree height measurement) is explored based on low emitted laser energy at high repetition frequency. Low energy approach drastical ly reduces the laser induced risks. Altimetry performances meet preliminary science requirements . The proposed instrument design is compatible with a space mission

Quantitative risk assessment of FMDV introduction in a FMD free country through bone-in beef and offal importation from a FMD free with vaccination country/zone

Quantitative risk assessment was used to estimate the risk of introducing foot-and-mouth disease (FMD) through bone-in beef from Argentina (FMD-free with vaccination status) into other FMD-free countries. A stochastic model was built to characterize all the steps from primary production to bone-in beef export and introduction into an FMD-free country. The probability that bone-in beef from at least one animal infected with the FMD virus (FMDV) was exported during a year was 5.27 × 10−3 (95% CI <10−10 – 5.19 x 10–2) or in other words one case in 190 years. The risk of FMDV introduction was sensitive to the probability of an outbreak occurring in Argentina (r [Spearman´s rank correlation] = 0.99) and the number of herds affected during an outbreak (r = 0.10). Additionally, the probability that susceptible animals in the importing country came into contact with infective material (bones) and generated an outbreak was 6.16 × 10−4 (95% CI <10−10 – 6.20 ×10−3) or one FMD outbreak every 1623 years on average. Based on the quantitative risk assessment results, the probability of FMDV introduction into a FMD-free country where vaccination is not practiced from a FMD-free country where vaccination is practiced associated with bone-in beef trade from Argentina was negligible. The risk of an FMD outbreak caused by the potential introduction of the FMDV was associated with the existing conditions in the country. Thus, maintaining the FMD-free status with or without vaccination would not be relevant.Fil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Durrieu, M.. No especifíca;Fil: Van Gelderen, C. J.. No especifíca;Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Schudel, A.. No especifíca

Évaluation des risques écologiques causés par des matériaux de dragage: roposition d'une approche adaptée aux dépôts en gravière en eau

Une procédure d'évaluation des risques pour l'écosystème aquatique engendrés par un dépôt de matériaux de dragage dans une gravière type a été élaborée, et testée avec des échantillons de sédiments d'un canal du Nord-Est de la France. La procédure comporte une étape d'évaluation sommaire des risques, à partir de quotients des concentrations mesurées par les critères de danger correspondants, et une étape d'évaluation détaillée où des essais de toxicité et de lixiviation en colonnes sont mis en œuvre. Le scénario testé retient trois hypothèses, qui concernent (a) les effets sur les peuplements d'invertébrés benthiques, représentés notamment par Hyalella azteca et Chironomus riparius, (b) les effets sur les peuplements d'organismes pélagiques, représentés par Chlorella vulgaris, Ceriodaphnia dubia, et Brachionus calyciflorus, et (c) la pollution de la nappe alluviale associée. Différentes modalités d'exposition (essais normalisés, microcosmes) ont été testées. Dans le contexte particulier des trois sédiments étudiés, ces hypothèses se sont avérées plus ou moins discriminantes, la pollution de la nappe étant la plus sensible. Des améliorations de la procédure doivent être envisagées qui concernent à la fois la formulation des hypothèses (risques à court et long terme sur les organismes pélagiques), et les protocoles d'essai, tant pour les organismes du sédiment (rôle de la nourriture notamment) que pour les essais de lixiviation en colonnes.When contaminated by metals or synthetic organic compounds, dredged sediments may have negative impacts on receiving ecosystems. Therefore, there is a need for an operational risk assessment approach. Such a framework is proposed for dredged material deposits in open gravel quarries, which is a rather common means of disposal in France. The first step of the assessment relies upon chemical characterisation of the sediments; the resulting concentrations are divided by "probable effect concentrations" and pooled together, in order to calculate a global hazard quotient. According to the value of this quotient, several decisions can be taken: (a) undertake a detailed risk assessment, (b) dispose of the materials without further constraints, or (c) in case of uncertainty, do some biological testing (with Hyalella azteca and Chironomus riparius) in order to allow decisions. The second step is a detailed ecological risk assessment. Three different assessment endpoints have been proposed, which are (1) the deposit should have no effect on the structure and abundance of benthic invertebrates in the quarry, (2) it should have no long term effect on pelagic species, and (3) it should not cause groundwater pollution, as such quarries are in fact cross sections of shallow alluvial groundwater aquifers. A fourth assessment endpoint should be introduced, regarding health risks for recreational uses, including fishing, but this endpoint was not implemented in the current version of the approach. The analysis phase includes aquatic bioassays (bacteria - Metplate TM-, algae, microcrustaceans Ceriodaphnia dubia, rotifers Brachionus calyciflorus), and leaching assays in columns under ascendant flow.The proposed approach was tested with 3 sediments from a canal located in the north-eastern region of France. Microcosm assays were introduced in parallel to the proposed tests, in order to explore alternatives to standardised bioassays. According to their hazard quotient, the 3 sediments showed a contamination gradient; one of them should not have entered the detailed risk assessment phase, while another would have been further tested with H. azteca and C. riparius. In that case, this latter sediment would not have entered the detailed phase either, as it was not toxic to these species. However, the detailed risk assessment approach was applied to the three sediments, so as to test completely the relevance of the framework.The three sediments were not significantly toxic to either C. riparius or H. azteca. However, some effects were observed in microcosms, including genotoxicity to molluscs. In this case, no risk characterisation could be made. Pore waters extracted from the three sediments were not toxic or slightly toxic to bacteria, algae, and C. dubia; an EC10 value could be determined only for B. calyciflorus. Therefore, due to exposure calculations, it seems there is a risk to pelagic species. However, as 3 bioassays out of 4 were negative or inconclusive, a refinement step would seem to be necessary. The highest concentrations of cadmium, copper, chromium, nickel and zinc were measured in the first lixiviates of the most contaminated sediment. Yet, even in that case, the total extracted fraction remained less than 10% of the total load. This fraction was below 1% for the other sediments, whatever the metal. Maximum concentrations and predicted concentrations at 1 year were compared to drinking water standards. This comparison showed a real risk of degrading groundwater quality for that most contaminated sediment, and a transient risk due to cadmium and nickel for the following one on the contamination gradient.Considering these results, the design of the first step of the proposed assessment approach may be discussed, as one sediment which would not have been assessed in depth according to its hazard quotient did show a risk to pelagic species. This discrepancy underlines the fact that some sediment toxicity may exist below the lowest threshold. As it would be unrealistic to enter systematically into detailed risk assessments, the proposed thresholds in the decision diagram must be pragmatic compromises rather than absolutely safe boundaries. Moreover, protocol improvements are needed for sediment toxicity bioassays. Chronic endpoints are preferable, as they are more sensitive and more relevant. Another issue is related to the role of additional food: not adding food may increase the apparent toxicity, but the sediment organic content, which is an alternative food source, may also be a contaminant carrier. Furthermore, the second assessment endpoint (risk to pelagic species) should be reformulated, as it includes in fact two different questions. Short-term risks related to the deposition phase could be assessed with standardised bioassays like C. dubia survival and reproduction and algal growth, while longer term risks related to contaminant diffusion could be assessed with microcosms. Genotoxic effects were observed at rather high levels, as compared to published results. This result stresses the interest of introducing sensitive and early markers in the risk assessment process, although their real meaning for ecosystems is not yet fully elucidated. Finally, the leaching tests in columns are not completely satisfactory, as the column filling implies that one must first dry the sediments, which will alter their structure. Other application trials along with field validation studies should be carried out prior to the introduction of this scenario in operational or regulatory frameworks

Transfer of particulate matter from the Northwestern Mediterranean continental margin: Variability and controlling factors

International audienceLong-term observations of monthly downward particle fluxes and hourly currents and temperaturewere initiated in 1993 in two canyons of the continental margin of the Gulf of Lion. The goals of thissurvey were to estimate its contribution to the CO 2 global budget and to understand the role offorcing factors in the control of present-day particle exchange across this margin. A previousstatistical analysis of the long-term time series suggested that variability in the transfer of particulatematter to the deep ocean could be the result of the effect of the meandering of the Northern Currentand by dense water formation in winter rather than variations in the sources of matter. Numericalsimulations have been carried out to consider these hypotheses. A model is used to examine theimpact of local atmospheric forcing (wind stress, heat fluxes, precipitation–evaporation budget) onthe variability of the oceanic circulation and of mass fluxes within the canyons from December toApril, for five consecutive years between 1996 and 2001. Results show an east-west gradient of massexport on the shelf and a positive correlation between anomalies of dense water formation rates andinterannual variability of particle fluxes. However, in the eastern part of the Gulf, the simulated massexport from the shelf is not significant, even during a winter of strong convection, when the measuredparticle fluxes are at maxima. Moreover, although the model suggests that the dense water formationcould be the major hydrodynamic forcing factor, this process is not sufficient to completely explainthe space and time variations of observed particle fluxes, especially at depth

Integration of formal fault analysis in ASSERT: Case studies and lessons learnt

International audienceThe ASSERT European Integrated Project (Automated proof-based System and Software Engineering for Real-Time systems; EC FP6, IST-004033) has investigated, elaborated and experimented advanced methods based on the AltaRica language and support tool OCAS for architecture and fault approach propagation description analysis, and integrated in the complete ASSERT process. The paper describes lessons learnt from three case studies: safety critical spacecraft, autonomous deep exploration spacecraft, and civil aircraft

Major consequences of an intense dense shelf water cascading event on deep-sea benthic trophic condtions and meiofaunal biodiversity

Numerous submarine canyons around the world are preferential conduits for episodic dense shelf water cas- cading (DSWC), which quickly modifies physical and chem- ical ambient conditions while transporting large amounts of material towards the base of slope and basin. Observations conducted during the last 20 yr in the Lacaze-Duthiers and Cap de Creus canyons (Gulf of Lion, NW Mediterranean Sea) report several intense DSWC events. The effects of DSWC on deep-sea ecosystems are almost unknown. To in- vestigate the effects of these episodic events, we analysed changes in the meiofaunal biodiversity inside and outside the canyon. Sediment samples were collected at depths varying from ca. 1000 to >2100m in May 2004 (before a major event), April 2005 (during a major cascading event) and in October 2005, August 2006, April 2008 and April 2009 (af- ter a major event). We report here that the late winter–early spring 2005 cascading led to a reduction of the organic mat- ter contents in canyon floor sediments down to 1800 m depth, whereas surface sediments at about 2200 m depth showed an increase. Our findings suggest that the nutritional material re- moved from the shallower continental shelf, canyon floor and flanks, and also the adjacent open slope was rapidly trans- ported to the deep margin. During the cascading event the meiofaunal abundance and biodiversity in the studied deep- sea sediments were significantly lower than after the event. Benthic assemblages during the cascading were significantly different from those in all other sampling periods in both the canyon and deep margin. After only six months from the cessation of the cascading, benthic assemblages in the impacted sediments were again similar to those observed in other sampling periods, thus illustrating a quick recovery. Since the present climate change is expected to increase the intensity and frequency of these episodic events, we anticipate that they will increasingly affect benthic bathyal ecosys- tems, which may eventually challenge their resilience

Conductometric biosensor based on whole-cell microalgae for assessment of heavy metals in wastewater

Whole-cell Chlorella vulgaris conductometric biosensors consisting of gold planar interdigitated electrodes and sol-gel algal membranes have been used for assessment of heavy-metal ions in water. These analytes act as algal alkaline phosphatase inhibitors. Enzyme residual activity has been measured in Tris-nitrate buffer in the presence of Mg²⁺ ions as activator. Operating conditions of this biosensor have been optimized and its characteristics are discussed. Detection limits are about 1 ppb for Cd²⁺, Co²⁺, Ni²⁺, Pb²⁺ and 10 ppb for Zn²⁺. The storage stability of the biosensor in buffer solution at 4 oC is more than 40 days. The biosensor has been used to assess wastewater pollution.Описан биосенсор для оценки содержания тяжелых металлов в воде. Для его создания клетки Chlorella vulgaris иммобилизировали на золотых планарных гребенчатых электродах с помощью золь–гель технологии. Тяжелые металлы являются ингибиторами щелочной фосфатазы. Остаточную активность иммобилизованного фермента измеряли в трис-нитратном буферном растворе в присутствии активатора: ионов магния. Оптимизированы рабочие характеристики биосенсора. Нижняя граница определения составляла 1 млрд ⁻¹ для Cd²⁺, Co²⁺, Ni²⁺, Pb²⁺ и 10 млрд ⁻¹ для Zn²⁺. Срок хранения биосенсора в буферном растворе при температуре 4 оC составляет более 40 дней. Биосенсор использован для оценки загрязнения тяжелыми металлами сточных вод.Описано біосенсор для оцінки вмісту важких металів у воді. Для його створення клітини Chlorella vulgaris іммобілізували на золотих планарних гребінчастих електродах за допомогою золь–гель технології. Важкі метали є інгібіторами лужної фосфатази. Залишкову активність іммобілізованого ферменту вимірювали в трис-нітратному буферному розчині за присутності активатора – іонів магнію. Оптимізовано робочі характеристики біосенсора. Нижня межа визначення складала 1 млрд ⁻¹ для Cd²⁺, Co²⁺, Ni²⁺, Pb²⁺ та 10 млрд ⁻¹ для Zn²⁺. Термін зберігання біосенсора у буферному розчині за температури 4 оC був більшим, ніж 40 діб. Біосенсор використано для оцінки забруднення важкими металами стічних вод

Quality of life: psychological symptoms-effects of a 2-month healthy diet and nutraceutical intervention; a randomized, open-label intervention trial (RISTOMED)

Depression symptoms and lower health-related quality of life (HRQoL) are associated with inflammation. This multicenter dietary intervention was shown to reduce inflammation in older people. This was the main outcome. Here, we describe the effects on HRQoL, anxiety, and depressive symptoms according to inflammation status. Overall, 125 healthy older subjects (65-80 year) were recruited (Italy, France, and Germany) and randomized into four arms (A, Healthy diet (HD); B, HD plus De Simone Formulation probiotic blend; C, HD plus AISA d-Limonene; D, HD plus Argan oil). The HD was weight maintaining, rich in antioxidant vitamins, polyphenols, polyunsaturated fatty acids (n6: n3 ratio = 3:1), and fiber. Data on inflammatory parameters, mental (MCS) and physical (PCS) component summaries of HRQoL (SF-36), anxiety symptoms (STAI state), and depressive symptoms (CES-D) were collected before and after 56 days of intervention. Body fat mass proportion (BFM) was considered a co-variable. A decrease of CES-D score was seen in the four arms (A: -40.0%, p = 0.001; B: -32.5%, p = 0.023; C: -42.8%, p = 0.004; and D: -33.3%, p = 0.21). Within the subgroups of subjects with medium/high inflammation a similar decrease in CES-D score occurred in all groups (A: -44.8%, p = 0.021; B, -46.7%, p = 0.024; C, -52.2%, p = 0.039; D, -43.8%, p = 0.037). The effect of interventions on CES-D was not related to baseline inflammation. MCS-HRQoL improved in A and C. There was no change in anxiety or PCS-HRQoL. In this trial with no control group, a decrease in depressive symptoms in healthy older volunteers was observed after a 2-month healthy diet intervention, independently of inflammation but with possible limitations due to participation