Porcine vas deferens luminal pH is acutely increased by systemic xylazine administration

Data are accumulating to demonstrate that pH regulation in the male reproductive tract has a vital role in modulating sperm cell fertilizing capacity and, therefore, male fertility. Bicarbonate uptake by sperm cells is required for the achievement of motility levels required for fertilization. Vas deferens epithelial cells can carry out measurable bicarbonate secretion, but the available literature to date reports that the vas deferens luminal content is typically acidic. This study aimed to determine pH in the boar vas deferens lumen and whether modulatory mechanisms exist for regulation of pH in this compartment of the male reproductive tract. A fiber-optic pH probe was used to assess pH in the vas deferens of anesthetized adult boars. The mean pH, derived from multiple measurements at variable positions along the vas deferens lumen, was 7.39 ± 0.09. Furthermore, administration of xylazine, an alpha-2 adrenergic receptor agonist, rapidly (< 10 min) alkalinized the vas deferens lumen in most cases. Since the duct was transected proximal to the site of measurements, the observations rule out the possibility that alkalinization resulted from secretion in more proximal portions of the duct. These results indicate that the boar vas deferens lumen can be alkaline, and suggest that porcine vas deferens epithelia increase net bicarbonate secretion in vivo, following systemic alpha-2 adrenergic stimulation. This secretory response greatly changes the luminal environment to which sperm cells are exposed, which will initiate or enhance motility, and is expected to modulate male fertility

Opening the black box: soil microcosm experiments reveal soot black carbon short-term oxidation and influence on soil organic carbon mineralisation

Soils hold three quarters of the total organic carbon (OC) stock in terrestrial ecosystems and yet we fundamentally lack detailed mechanistic understanding of the turnover of major soil OC pools. Black carbon (BC), the product of the incomplete combustion of fossil fuels and biomass, is ubiquitous in soils globally. Although BC is a major soil carbon pool, its effects on the global carbon cycle have not yet been resolved. Soil BC represents a large stable carbon pool turning over on geological timescales, but research suggests it can alter soil biogeochemical cycling including that of soil OC. Here, we established two soil microcosm experiments: experiment one added 13C OC to soil with and without added BC (soot or biochar) to investigate whether it suppresses OC mineralisation; experiment two added 13C BC (soot) to soil to establish whether it is mineralised in soil over a short timescale. Gases were sampled over six-months and analysed using isotope ratio mass spectrometry. In experiment one we found that the efflux of 13C OC from soil decreased over time, but the addition of soot to soil significantly reduced the mineralisation of OC from 32% of the total supplied without soot to 14% of the total supplied with soot. In contrast, there was not a significant difference after the addition of biochar in the flux of 13C from the OC added to the soil. In experiment two, we found that the efflux 13C from soil with added 13C soot significantly differed from the control, but this efflux declined over time. There was a cumulative loss of 0.17% 13C from soot over the experiment. These experimental results represent a step-change in understanding the influence of BC continuum on carbon dynamics, which has major consequences for the way we monitor and manage soils for carbon sequestration in future

CCR8 Expression Defines Tissue-Resident Memory T Cells in Human Skin

Human skin harbors two major T cell compartments of equal size that are distinguished by expression of the chemokine receptor CCR8. In vitro studies have demonstrated that CCR8 expression is regulated by TCR engagement and the skin tissue microenvironment. To extend these observations, we examined the relationship between CCR8+ and CCR8− skin T cells in vivo. Phenotypic, functional, and transcriptomic analyses revealed that CCR8+ skin T cells bear all the hallmarks of resident memory T cells, including homeostatic proliferation in response to IL-7 and IL-15, surface expression of tissue localization (CD103) and retention (CD69) markers, low levels of inhibitory receptors (programmed cell death protein 1, Tim-3, LAG-3), and a lack of senescence markers (CD57, killer cell lectin-like receptor subfamily G member 1). In contrast, CCR8− skin T cells are heterogeneous and comprise variable numbers of exhausted (programmed cell death protein 1+), senescent (CD57+, killer cell lectin-like receptor subfamily G member 1+), and effector (T-bethi, Eomeshi) T cells. Importantly, conventional and high-throughput sequencing of expressed TCR β-chain (TRB) gene rearrangements showed that these CCR8-defined populations are clonotypically distinct, suggesting unique ontogenies in response to separate antigenic challenges and/or stimulatory conditions. Moreover, CCR8+ and CCR8− skin T cells were phenotypically stable in vitro and displayed similar levels of telomere erosion, further supporting the likelihood of a nonlinear differentiation pathway. On the basis of these results, we propose that long-lived memory T cells in human skin can be defined by the expression of CCR8

Five years of SGR 1900+14 observations with BeppoSAX

We present a systematic analysis of all the BeppoSAX data of the soft gamma-ray repeater SGR 1900+14: these observations allowed us to study the long term properties of the source quiescent emission. In the observation carried out before the 1998 giant flare the spectrum in the 0.8-10 keV energy range was harder and there was evidence for a 20-150 keV emission, possibly associated with SGR 1900+14. This possible hard tail, if compared with the recent INTEGRAL detection of SGR 1900+14, has a harder spectrum (power-law photon index ~1.6 versus ~3) and a 20-100 keV flux ~4 times larger. In the last BeppoSAX observation (April 2002), while the source was entering the long quiescent period that lasted until 2006, the 2-10 keV flux was ~25% below the historical level. We also studied in detail the spectral evolution during the 2001 flare afterglow. This was characterized by a softening that can be interpreted in terms of a cooling blackbody-like component.Comment: Accepted for publication on Astronomy and Astrophysics on August 31, 200

Nebuliser therapy in the intensive care unit

The relationship between identity, lived experience, sexual practices and the language through which these are conveyed has been widely debated in sexuality literature. For example, ‘coming out’ has famously been conceptualised as a ‘speech act’ (Sedgwick 1990) and as a collective narrative (Plummer 1995), while a growing concern for individuals’ diverse identifications in relations to their sexual and gender practices has produced interesting research focusing on linguistic practices among LGBT-identified individuals (Leap 1995; Kulick 2000; Cameron and Kulick 2006; Farqhar 2000). While an explicit focus on language remains marginal to literature on sexualities (Kulick 2000), issue of language use and translation are seldom explicitly addressed in the growing literature on intersectionality. Yet intersectional perspectives ‘reject the separability of analytical and identity categories’ (McCall 2005:1771), and therefore have an implicit stake in the ‘vernacular’ language of the researched, in the ‘scientific’ language of the researcher and in the relationship of continuity between the two. Drawing on literature within gay and lesbian/queer studies and cross-cultural studies, this chapter revisits debates on sexuality, language and intersectionality. I argue for the importance of giving careful consideration to the language we choose to use as researchers to collectively define the people whose experiences we try to capture. I also propose that language itself can be investigated as a productive way to foreground how individual and collective identifications are discursively constructed, and to unpack the diversity of lived experience. I address intersectional complexity as a methodological issue, where methodology is understood not only as the methods and practicalities of doing research, but more broadly as ‘a coherent set of ideas about the philosophy, methods and data that underlie the research process and the production of knowledge’ (McCall 2005:1774). My points are illustrated with examples drawn from my ethnographic study on ‘lesbian’ identity in urban Russia, interspersed with insights from existing literature. In particular, I aim to show that an explicit focus on language can be a productive way to explore the intersections between the global, the national and the local in cross-cultural research on sexuality, while also addressing issues of positionality and accountability to the communities researched

Evaluation of the relative potential for contact and doffing transmission of SARS-CoV-2 by a range of personal protective equipment materials

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—the causative agent of coronavirus disease 2019 (COVID-19)—has caused a global public health emergency. Personal protective equipment (PPE) is the primary defence against viral exposure in healthcare and community settings. However, the surfaces of PPE materials may trap virus for contact transmission or through laden aerosols generated during removal of PPE, through cleaning or during movement. In this study, the relative efficacy of current PPE materials in terms of virion adsorption to materials and their antiviral potency, has been evaluated on a wide range of PPE for the first time, including four polymer glove types, two types of scrubs, apron material, a mask, visor and a selection of other commercial polymers and products. Although differences in virion adsorption to the test materials were observed, none of the existing polymer-based PPE resulted in more than tenfold reduction in the SARS-CoV-2 titre within either 10min or 30min contact period. The wettability and surface chemistry of the test materials were analysed to investigate any correlations with their surface physicochemical properties. While no correlation was found between wettability and viral retention under air flow challenge, one secondary ion of m/z 101.03 (+) and three secondary ions of m/z 31.98 (−), 196.93 (−) and 394.33 (+) in ToF–SIMS data of the test materials showed positive and negative correlations with the viral retention, respectively, which was identified by PLS regression model, suggesting that the surface chemistry plays a role in determining the extent of virion adsorption. Our findings outline the material aspects that influence the efficacy of current PPE against SARS-CoV-2 transmission and give suggestions on the development of novel simple polymer-based PPE for better infection protection

Hadron Helicity Violation in Exclusive Processes: Quantitative Calculations in Leading Order QCD

We study a new mechanism for hadronic helicity flip in high energy hard exclusive reactions. The mechanism proceeds in the limit of perfect chiral symmetry, namely without any need to flip a quark helicity. The fundamental feature of the new mechanism is the breaking of rotational symmetry of the hard collision by a scattering plane in processes involving independent quark scattering. We show that in the impulse approximation there is no evidence for of the helicity violating process as the energy or momentum transfer $Q^2$ is increased over the region 1 GeV^2 < Q^2 < 100 GeV^2. In the asymptotic region Q^2> 1000 GeV^2, a saddle point approximation with doubly logarithmic accuracy yields suppression by a fraction of power of Q^2. ``Chirally--odd" exclusive wave functions which carry non--zero orbital angular momentum and yet are leading order in the high energy limit, play an important role.Comment: uuencoded LaTeX file (21 pages) and PostScript figure

The effects of arbuscular mycorrhizal fungal colonisation on nutrient status, growth, productivity, and canker resistance of apple (Malus pumila)

We assess whether arbuscular mycorrhizal fungi (AMF) improve growth, nutritional status, phenology, flower and fruit production, and disease resistance in woody perennial crops using apple (Malus pumila) as a study system. In a fully factorial experiment, young trees were grown for 3 years with or without AMF (Funneliformis mosseae and Rhizophagus irregularis), and with industrial standard fertiliser applications or restricted fertiliser (10% of standard). We use two commercial scions (Dabinett and Michelin) and rootstocks (MM111 and MM106). Industrial standard fertiliser applications reduced AMF colonisation and root biomass, potentially increasing drought sensitivity. Mycorrhizal status was influenced by above ground genotypes (scion type) but not rootstocks, indicating strong interactions between above and below ground plant tissue. The AMF inoculation significantly increased resistance to Neonectria ditissima, a globally economically significant fungal pathogen of apple orchards, but did not consistently alter leaf nutrients, growth, phenology or fruit and flower production. This study significantly advances understanding of AMF benefits to woody perennial crops, especially increased disease resistance which we show is not due to improved tree nutrition or drought alleviation. Breeding programmes and standard management practises can limit the potential for these benefits

Is the lower return to immigrants’ foreign schooling a postarrival problem in Canada?

Published versionUsing the 2006 Canadian Census, this paper investigates the lower return to immigrants’ foreign education credentials after adjusting for their occupational matching in hosting labor markets. We develop two continuous indices that quantify the matching quality of the native-born in both horizontal (fields of study) and vertical (educational degrees) dimensions. This allows us to separate the effects of immigrants’ occupational attainment and their foreign schooling quality on wage earnings by measuring immigrants’ occupational match relative to that of native-born. Our findings indicate that the lack of portability in immigrants’ foreign credentials may not be addressed effectively by post arrival policies as the results show that a significant and persistent poor matching quality for internationally educated immigrants cannot substantiate the lower return to their foreign education credentials