Antifreeze Proteins in the Primary Urine of Larvae of the Beetle \u3cem\u3eDendroides canadensis\u3c/em\u3e

To avoid freezing while overwintering beneath the bark of fallen trees, Dendroides canadensis (Coleoptera: Pyrochroidae) larvae produce a family of antifreeze proteins (DAFPs) that are transcribed in specific tissues and have specific compartmental fates. DAFPs and associated thermal hysteresis activity (THA) have been shown previously in hemolymph and midgut fluid, but the presence of DAFPs has not been explored in primary urine, a potentially important site that can contain endogenous ice-nucleating compounds that could induce freezing. A maximum mean THA of 2.65±0.33°C was observed in primary urine of winter-collected D. canadensis larvae. THA in primary urine increased significantly through autumn, peaked in the winter and decreased through spring to levels of 0.2–0.3°C in summer, in a pattern similar to that of hemolymph and midgut fluid. THA was also found in hindgut fluid and excreted rectal fluid, suggesting that these larvae not only concentrate AFPs in the hindgut, but also excrete AFPs from the rectal cavity. Based on dafp transcripts isolated from Malpighian tubule epithelia, cDNAs were cloned and sequenced, identifying the presence of transcripts encoding 24 DAFP isoforms. Six of these Malpighian tubule DAFPs were known previously, but 18 are new. We also provide functional evidence that DAFPs can inhibit ice nucleators present in insect primary urine. This is potentially critical because D. canadensis larvae die if frozen, and therefore ice formation in any body fluid, including the urine, would be lethal

Design of LDPC Codes for Two-Way Relay Systems with Physical-Layer Network Coding

Cataloged from PDF version of article.This letter presents low-density parity-check (LDPC) code design for two-way relay (TWR) systems employing physical-layer network coding (PLNC). We focus on relay decoding, and propose an empirical density evolution method for estimating the decoding threshold of the LDPC code ensemble. We utilize the proposed method in conjunction with a random walk optimization procedure to obtain good LDPC code degree distributions. Numerical results demonstrate that the specifically designed LDPC codes can attain improvements of about 0.3 dB over off-the-shelf LDPC codes (designed for point-to-point additive white Gaussian noise channels), i.e., it is new code designs are essential to optimize the performance of TWR systems

Magnetic superelasticity and inverse magnetocaloric effect in Ni-Mn-In

Applying a magnetic field to a ferromagnetic Ni$_{50}$Mn$_{34}$In$_{16}$ alloy in the martensitic state induces a structural phase transition to the austenitic state. This is accompanied by a strain which recovers on removing the magnetic field giving the system a magnetically superelastic character. A further property of this alloy is that it also shows the inverse magnetocaloric effect. The magnetic superelasticity and the inverse magnetocaloric effect in Ni-Mn-In and their association with the first order structural transition is studied by magnetization, strain, and neutron diffraction studies under magnetic field.Comment: 6 pages, 8 figures. Published in the Physical Review

Magnetic superelasticity and inverse magnetocaloric effect in Ni-Mn-In

Applying a magnetic field to a ferromagnetic Ni$_{50}$Mn$_{34}$In$_{16}$ alloy in the martensitic state induces a structural phase transition to the austenitic state. This is accompanied by a strain which recovers on removing the magnetic field giving the system a magnetically superelastic character. A further property of this alloy is that it also shows the inverse magnetocaloric effect. The magnetic superelasticity and the inverse magnetocaloric effect in Ni-Mn-In and their association with the first order structural transition is studied by magnetization, strain, and neutron diffraction studies under magnetic field.Comment: 6 pages, 8 figures. Published in the Physical Review

Multiple-Resampling Receiver Design for OFDM Over Doppler-Distorted Underwater Acoustic Channels

Cataloged from PDF version of article.In this paper, we focus on orthogonal frequency-divisionmultiplexing (OFDM) receiver designs for underwater acoustic (UWA) channels with user- and/or path-specific Doppler scaling distortions. The scenario is motivated by the cooperative communications framework, where distributed transmitter/receiver pairs may experience significantly different Doppler distortions, as well as by the single-user scenarios, where distinct Doppler scaling factors may exist among different propagation paths. The conventional approach of front–end resampling that corrects for common Doppler scalingmay not be appropriatein such scenarios, rendering a post-fast-Fourier-transform (FFT) signal that is contaminated by user- and/or path-specific intercarrier interference. To counteract this problem, we propose a family of front–end receiver structures thatutilizemultiple-resampling (MR)branches,eachmatched to the Doppler scaling factor of a particular user and/or path. Following resampling, FFT modules transform the Doppler-compensated signals into the frequency domain for further processing through linear or nonlinear detection schemes. As part of the overall receiver structure, a gradient–descent approachis also proposed to refine the channel estimates obtained by standard sparse channel estimators. The effectiveness and robustness of the proposed receivers are demonstrated via simulations, as well as emulations based on real data collected during the 2010 Mobile Acoustic Communications Experiment (MACE10, Martha’s Vineyard, MA) and the 2008 Kauai Acomms MURI (KAM08, Kauai, HI) experiment

Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes

BACKGROUND: RNA interference (RNAi), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. Saccharomyces cerevisiae (baker's yeast) may represent an effective interfering RNA expression system that could be used directly for delivery of RNA pesticides to mosquito larvae. Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes. RESULTS: Experiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. The larvicide was also found to induce high levels of Aedes albopictus, Anopheles gambiae and Culex quinquefasciatus mortality. CONCLUSIONS: The results of these studies indicate that use of yeast interfering RNA larvicides targeting mosquito sema1a genes may represent a new biorational tool for mosquito control

Evaluation of localisation of mandibular foramen in patients with mandibular third molar teeth using cone-beam computed tomography

Background: It is important to know the correct anatomical location of the mandibular foramen to obtain successful anaesthesia of inferior alveolar nerve and to prevent injury to the mandibular vessels and nerve, during a variety of oral and maxillofacial surgical procedures. The aim of this study is to evaluate localisation of the mandibular foramen in patients with the third molars using cone-beam computed tomography (CBCT). Materials and methods: Cone-beam computed tomography was used to determine the location of the mandibular foramen in 67 patients (totally 99 sides) with unilateral or bilateral impacted mandibular third molars. Results: The distance from the posterior border of the mandibular ramus to mandibular foramen did not differ significantly among the other angulations. But the difference between vertical and horizontal angulation of the impactedmandibular third molars according to ‘fd’ values (the shortest distance between mandibular foramen and the posterior border of mandibular ramus) was found to be statistically significant (p < 0.05). Conclusions: The present study provides new information to the literature concerning relationship between the location of the mandibular foramen and the mandibular third molars

Benefits of Environmental Conditions for Growing Coriander in Banat Region, Serbia

As one of the oldest multi-purpose plants (spice, aromatic, honey and medicinal), coriander is widespread across Europe. Although in Serbia there are favorable conditions fir its growth and development, it is grown on relatively small areas. During both investigated years it took more than 1200 degrees C for transfer from vegetative to generative phase of development and over 2000 degrees C for it to be ready for harvesting. Coriander is a photophilic plant, which requires around 1000 hours of light from sowing to ripening.. As for humidity, coriander grows well, if there are more than 200 mm of rainfall during growing season. In 2009. and 2010., the experiment carried out at the experimental field in Ostojicevo (Banat, Vojvodina province, Serbia) monitored the effect of parameters mentioned above on development of coriander plants, seed yield and essential oil content. The average yields of 1866 kg ha(-1) (2009) and 2470 kg ha(-1) (2010), and relatively high content of essential oil (1,06 % in both years) indicate a great potential of this plant species in Serbia, which is, however, greatly dependent on environmental conditions during year

Target detection and classification in SAR images using region covariance and co-difference

In this paper, a novel descriptive feature parameter extraction method from synthetic aperture radar (SAR) images is proposed. The new approach is based on region covariance (RC) method which involves the computation of a covariance matrix whose entries are used in target detection and classification. In addition the region co-difference matrix is also introduced. Experimental results of object detection in MSTAR (moving and stationary target recognition) database are presented. The RC and region co-difference method delivers high detection accuracy and low false alarm rates. It is also experimentally observed that these methods produce better results than the commonly used principal component analysis (PCA) method when they are used with different distance metrics introduced. © 2009 SPIE