A practice-oriented model for pushover analysis of a class of timber-framed masonry buildings

Timber-Framed (TF) masonry is a structural system characterized by high complexity and diversity. Limited experimental and analytical research has been carried out so far to explore their earthquake response, partly due to the complexity of the problem and partly due to the scarcity of TF buildings across the world. Here, a new practice-oriented non-linear (NL) macro-model is presented for TF masonry structures, based on the familiar diagonal strut approach with NL axial hinges in the struts. The constitutive law for the hinges (axial force vs. axial deformation) is derived on the basis of an extensive parametric analysis of the main factors affecting the response of TF masonry panels subjected to horizontal loading. The parameters studied are related to the geometric features of the panel and the strength of wood as well as the connections of the timber elements. The parametric analysis is performed using a micro-model based on Hill-type plasticity and it is shown that in the studied X-braced walls the masonry infills do not make a significant contribution to the lateral load resistance. Empirical expressions are proposed for the yield and maximum displacement and shear of a horizontally loaded TF panel. The model is verified against available experimental data, and is found to capture well the envelopes of the experimental loops. The model is readily applicable to NL static analysis (pushover) analysis for the assessment of the lateral load capacity of TF masonry buildings, as the number of input parameters for deriving the constitutive law has been limited to only five

Still a Host of Hosts for Wolbachia: Analysis of Recent Data Suggests That 40% of Terrestrial Arthropod Species Are Infected

Wolbachia are intracellular bacteria that manipulate the reproduction of their arthropod hosts in remarkable ways. They are predominantly transmitted vertically from mother to offspring but also occasionally horizontally between species. In doing so, they infect a huge range of arthropod species worldwide. Recently, a statistical analysis estimated the infection frequency of Wolbachia among arthropod hosts to be 66%. At the same time, the authors of this analysis highlighted some weaknesses of the underlying data and concluded that in order to improve the estimate, a larger number of individuals per species should be assayed and species be chosen more randomly. Here we apply the statistical approach to a more appropriate data set from a recent survey that tested both a broad range of species and a sufficient number of individuals per species. Indeed, we find a substantially different infection frequency: We now estimate the proportion of Wolbachia-infected species to be around 40% which is lower than the previous estimate but still points to a surprisingly high number of arthropods harboring the bacteria. Notwithstanding this difference, we confirm the previous result that, within a given species, typically most or only a few individuals are infected. Moreover, we extend our analysis to include several reproductive parasites other than Wolbachia that were also screened for in the aforementioned empirical survey. For these symbionts we find a large variation in estimated infection frequencies and corroborate the finding that Wolbachia are the most abundant endosymbionts among arthropod species

Wolbachia pipientis associated with tephritid fruit fly pests: from basic research to applications

Members of the true fruit flies (family Tephritidae) are among the most serious agricultural pests worldwide, whose control and management demands large and costly international efforts. The need for cost-effective and environmentally friendly integrated pest management (IPM) has led to the development and implementation of autocidal control strategies. These approaches include the widely used sterile insect technique and the incompatible insect technique (IIT). IIT relies on maternally transmitted bacteria (namely Wolbachia) to cause a conditional sterility in crosses between released mass-reared Wolbachia-infected males and wild females, which are either uninfected or infected with a different Wolbachia strain (i.e., cytoplasmic incompatibility; CI). Herein, we review the current state of knowledge on Wolbachia-tephritid interactions including infection prevalence in wild populations, phenotypic consequences, and their impact on life history traits. Numerous pest tephritid species are reported to harbor Wolbachia infections, with a subset exhibiting high prevalence. The phenotypic effects of Wolbachia have been assessed in very few tephritid species, due in part to the difficulty of manipulating Wolbachia infection (removal or transinfection). Based on recent methodological advances (high-throughput DNA sequencing) and breakthroughs concerning the mechanistic basis of CI, we suggest research avenues that could accelerate generation of necessary knowledge for the potential use of Wolbachia-based IIT in area-wide integrated pest management (AW-IPM) strategies for the population control of tephritid pests.Instituto de GenéticaFil: Mateos, Mariana. Texas A&M University. Departments of Ecology and Conservation Biology, and Wildlife and Fisheries Sciences; Estados UnidosFil: Martinez Montoya, Humberto. Universidad Autónoma de Tamaulipas. Unidad Académica Multidisciplinaria Reynosa Aztlan. Laboratorio de Genética y Genómica Comparativa; MéxicoFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Guillén, Karina. El Colegio de la Frontera Sur; MéxicoFil: Morán-Aceves, Brenda M. El Colegio de la Frontera Sur; MéxicoFil: Toledo, Jorge. El Colegio de la Frontera Sur; MéxicoFil: Liedo, Pablo. El Colegio de la Frontera Sur; MéxicoFil: Asimakis, Elias D. University of Patras. Department of Environmental Engineering; GreciaFil: Doudoumis, Vangelis. University of Patras. Department of Environmental Engineering; GreciaFil: Kyritsis, Georgios A. University of Thessaly. Department of Agriculture Crop Production and Rural Environment. Laboratory of Entomology and Agricultural Zoology; GreciaFil: Papadopoulos, Nikos T. University of Thessaly. Department of Agriculture Crop Production and Rural Environment. Laboratory of Entomology and Agricultural Zoology; GreciaFil: Augustinos, Antonios A. Hellenic Agricultural Organization. Institute of Industrial and Forage Crops. Department of Plant Protection; GreciaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tsiamis, George. University of Patras. Department of Environmental Engineering; Greci

Challenging the Wigglesworthia, Sodalis, Wolbachia symbiosis dogma in tsetse flies : Spiroplasma is present in both laboratory and natural populations

Profiling of wild and laboratory tsetse populations using 16S rRNA gene amplicon sequencing allowed us to examine whether the “Wigglesworthia-Sodalis-Wolbachia dogma” operates across species and populations. The most abundant taxa, in wild and laboratory populations, were Wigglesworthia (the primary endosymbiont), Sodalis and Wolbachia as previously characterized. The species richness of the microbiota was greater in wild than laboratory populations. Spiroplasma was identified as a new symbiont exclusively in Glossina fuscipes fuscipes and G. tachinoides, members of the palpalis sub-group, and the infection prevalence in several laboratory and natural populations was surveyed. Multi locus sequencing typing (MLST) analysis identified two strains of tsetse-associated Spiroplasma, present in G. f. fuscipes and G. tachinoides. Spiroplasma density in G. f. fuscipes larva guts was significantly higher than in guts from teneral and 15-day old male and female adults. In gonads of teneral and 15-day old insects, Spiroplasma density was higher in testes than ovaries, and was significantly higher density in live versus prematurely deceased females indicating a potentially mutualistic association. Higher Spiroplasma density in testes than in ovaries was also detected by fluorescent in situ hybridization in G. f. fuscipe

Paul Baldassera et le mythe d'Hermès

Doudoumis Anélie. Paul Baldassera et le mythe d'Hermès. In: Cahiers du Centre d'Etudes Chypriotes. Volume 29, 1999. pp. 167-184