Introduction to The Special Issue: Advances in Methods and Measurement in Family Psychology

This special issue presents a collection of reports that highlight recent advances in methods and measurement and also shed light on the complexity of family psychology. The importance of theory in guiding solid family science is evident throughout these reports. The reports include guides for researchers who incorporate direct observation into their research protocols and the ever-expanding field of tele-health interventions. Advanced analytic approaches are offered in the areas of grid sequence analysis, latent fixed-effects models, and the Factors of Curves Model (FOCUS). These sophisticated analytic approaches may be applied to advance systemic thinking in family psychology. The last set of articles illustrate how complex and innovative methodologies are applied to address important societal issues. Work experiences and marital relationships in African American couples address the importance of spillover effects in contemporary families. The creation of biobehavioral plasticity index has the potential to inform gene x environment contributions to family functioning. Finally, the unique methodological issues that are particularly germane to the diverse nature of stepfamilies and nonresident fathers are addressed. We hope that readers of this special issue will return to these reports as resources and examples of theory-driven methods and measurements

Histological characteristics of singleton placentas delivered before the 28th week of gestation

The placenta is a record of the fetal environment and its examination may provide information about the baby’s subsequent growth and development. We describe the histological characteristics of 947 singleton placentas from infants born between 23 and 27 weeks gestation

Transcriptional and Linkage Analyses Identify Loci that Mediate the Differential Macrophage Response to Inflammatory Stimuli and Infection

Macrophages display flexible activation states that range between pro-inflammatory (classical activation) and anti-inflammatory (alternative activation). These macrophage polarization states contribute to a variety of organismal phenotypes such as tissue remodeling and susceptibility to infectious and inflammatory diseases. Several macrophage- or immune-related genes have been shown to modulate infectious and inflammatory disease pathogenesis. However, the potential role that differences in macrophage activation phenotypes play in modulating differences in susceptibility to infectious and inflammatory disease is just emerging. We integrated transcriptional profiling and linkage analyses to determine the genetic basis for the differential murine macrophage response to inflammatory stimuli and to infection with the obligate intracellular parasite Toxoplasma gondii. We show that specific transcriptional programs, defined by distinct genomic loci, modulate macrophage activation phenotypes. In addition, we show that the difference between AJ and C57BL/6J macrophages in controlling Toxoplasma growth after stimulation with interferon gamma and tumor necrosis factor alpha mapped to chromosome 3, proximal to the Guanylate binding protein (Gbp) locus that is known to modulate the murine macrophage response to Toxoplasma. Using an shRNA-knockdown strategy, we show that the transcript levels of an RNA helicase, Ddx1, regulates strain differences in the amount of nitric oxide produced by macrophage after stimulation with interferon gamma and tumor necrosis factor. Our results provide a template for discovering candidate genes that modulate macrophage-mediated complex traits

Eddy covariance measurements of the sea spray aerosol flux over the open ocean

Direct eddy covariance measurements of size-segregated sea spray aerosol fluxes over the open Atlantic Ocean are presented, along with a source function derived from them for a wind speed range of 4 to 18 m s -1 and a size range of 0.176 < R 80 < 6.61 μm. This is in broad agreement with other recent estimates of the source function over this size range but shows a more rapid decrease with size above R 80 = 2 μm than most other functions. The measurements were made during a 3 week cruise in the North Atlantic as part of the UK contribution to the international Surface Ocean-Lower Atmosphere Study (SOLAS) program. They utilized the new high-rate Compact Lightweight Aerosol Spectrometer Probe (CLASP), providing a 16-channel size spectrum (0.17 < R amb < 9.5 μm) at 10 Hz, collocated with a sonic anemometer. The measurements demonstrate the high variability in sea spray aerosol flux compared with other air-sea fluxes, both between individual estimates and in the scales contributing to the flux