Partitioning of starter bacteria and added exogenous enzyme activities between curd and whey during Cheddar cheese manufacture

peer-reviewedPartitioning of starter bacteria and enzyme activities was investigated at different stages of Cheddar cheese manufacture using three exogenous commercial enzyme preparations added to milk or at salting. The enzyme preparations used were: Accelase AM317, Accelase AHC50, Accelerzyme CPG. Flow cytometric analysis indicated that AHC50 or AM317 consisted of permeabilised or dead cells and contained a range of enzyme activities. The CPG preparation contained only carboxypeptidase activity. Approximately 90% of starter bacteria cells partitioned with the curd at whey drainage. However, key enzyme activities partitioned with the bulk whey in the range of 22%–90%. An increased level of enzyme partitioning with the curd was observed for AHC50 which was added at salting, indicating that the mode of addition influenced partitioning. These findings suggest that further scope exists to optimise both bacterial and exogenous enzyme incorporation into cheese curd to accelerate ripening.Department of Agriculture, Food and the Marin

Any Time? Any Place? The impact on student learning of an on-line learning environment.

Original paper can be found at: http://www.actapress.com/Content_of_Proceeding.aspx?proceedingID=292#pages Copyright ACTA Press [Full text of this paper is not available in the UHRA]An increasing number of HE institutions are adopting virtual and managed learning environments (VLEs and MLEs), which offer flexible access to on-line learning materials all day and every day. There are multiple claims about e-learning enhancing learning and teaching (eg. [1] Britain and Liber, 1999; [2]Conole, 2002; [4]Allen, 2003; [5]Littlejohn and Higginson, 2003) such as supporting active learning, facilitative rather than didactic teaching and increased student motivation but these are not pre determined outcomes. Much depends on how lecturers use the available technology and how students respond to that use. This paper reports on a research project which has evaluated the students' own experience of on-line learning at the University of Hertfordshire. Using its own institution-wide MLE (StudyNet) academic staff at the university have been able to offer students on-line access to their study material from September 2001. Activities available for students using StudyNet include participating in discussion forums, using formative assessment materials and accessing journal articles as well as viewing and downloading courseware for each of their courses. Students were invited to participate in a questionnaire and focus groups to identify the characteristics of the on-line learning environment which benefited their learning

The social impact of accounting processes on benefit claimants in the UK

The accounting processes of categorisation and classification are inherent in modern-day welfare systems, though little has been done to investigate the link these have to the social consequences for benefit claimants within these systems. This paper uses research from both primary and secondary sources to show how UK welfare reform has affected claimants and their inalienable human rights since its introduction in 2012. The data gathered for this work combine face-to-face interview data with press releases, and data and reports compiled and published both by the government and independent bodies. Face-to-face interviews were conducted with two illustrative participants, who were identified as being excellent examples of individuals with a close working knowledge of the welfare system. In addition to the primary data gathered, several sources of secondary data are used within the analysis to identify facts, figures and quotations from reliable government sources. Our analysis uncovers that the accounting processes inherent in the system have helped foster a culture of stigmatisation, food bank dependency and financial and emotional hardship for vulnerable welfare claimants in today’s society

Statistical estimation of trailing edge noise from finite wall-mounted airfoils

The 2016 Joint Conference of The Australian Acoustical Society and The Acoustical Society of New Zealand. Acoustics2016 - The Second Australasian Acoustical Societies ConferenceIt is important to be able to accurately model the flow and noise generated by finite wall-mounted airfoil flows because of the many engineering applications in which these flows occur. One method for predicting turbulent trailing edge noise is the Reynolds-averaged Navier-Stokes based statistical noise model (RSNM) of Doolan et al. (Proceedings of 20th International Congress on Acoustics, ICA 2010). The RSNM method has previously been used successfully on a range of two-dimensional geometry-flow cases. In this paper a new turbulent velocity cross-spectrum model and improved implementation are proposed to allow the RSNM method to be used to effectively and efficiently predict turbulent trailing edge noise from more complex three-dimensional cases. Reynolds-averaged Navier-Stokes (RANS) simulations of a series of wing-in-junction cases are used in combination with the developed acoustic model to predict the far-field noise and compared against experimental noise measurements.Jesse Coombs, Con Doolan, Anthony Zander, Danielle Moreau and Laura Brook

The relationship between early pregnancy dietary intakes and subsequent birthweight and neonatal adiposity

Background: Maternal nutrition intakes may influence neonatal birthweight and adiposity; however, inconsistencies within the literature exist. The relationships between maternal dietary intakes in early pregnancy and both birthweight and neonatal adiposity requires elucidation. This study examined the relationship between early pregnancy dietary intakes and subsequent birthweight and neonatal adiposity. Methods: Women were recruited at their convenience after sonographic confirmation of a singleton pregnancy. Women completed a Willet food frequency questionnaire evaluating habitual food and nutrient intakes at their first antenatal visit. Neonatal body composition was measured using air-displacement plethysmography. Results: Of the 385 mother-neonate dyads, mean maternal age was 30.8 ± 5.3 years, mean Body Mass Index (BMI) was 24.5 ± 4.8 kg/m2 and 41.8% (n = 161) were nulliparous. There were no relationships between maternal food intakes and birthweight (P \u3e 0.05) (n = 385). On multivariable analysis there was a positive relationship between polyunsaturated fat and neonatal fat mass index (FMI) (beta = 0.015, 95% CI = 0.002-0.028, P = 0.04) (n = 80). Conclusion: Dietary intakes of polyunsaturated fat in early pregnancy are positively associated with neonatal FMI at birth on multivariable analysis. Further longitudinal studies need to explore this association and the long-term implications for the neonate

Inter-comparison of relative stopping power estimation models for proton therapy

Theoretical stopping power values were inter-compared for the Bichsel, Janni, ICRU and Schneider relative stopping power (RSP) estimation models, for a variety of tissues and tissue substitute materials taken from the literature. The RSPs of eleven plastic tissue substitutes were measured using Bragg peak shift measurements in water in order to establish a gold standard of RSP values specific to our centre's proton beam characteristics. The theoretical tissue substitute RSP values were computed based on literature compositions to assess the four different computation approaches. The Bichsel/Janni/ICRU approaches led to mean errors in the RSP of  −0.1/+0.7/−0.8%, respectively. Errors when using the Schneider approach, with I-values from the Bichsel, Janni and ICRU sources, followed the same pattern but were generally larger. Following this, the mean elemental ionisation energies were optimized until the differences between theoretical RSP values matched measurements. Failing to use optimized I-values when applying the Schneider technique to 72 human tissues could introduce errors in the RSP of up to  −1.7/+1.1/−0.4% when using Bichsel/Janni/ICRU I-values, respectively. As such, it may be necessary to introduce an additional step in the current stoichiometric calibration procedure in which tissue insert RSPs are measured in a proton beam. Elemental I-values can then optimized to match these measurements, reducing the uncertainty when calculating human tissue RSPs

A Role for Antimicrobial Stewardship in Clinical Sepsis Pathways: A Prospective Interventional Study

© 2017 by The Society for Healthcare Epidemiology of America. All rights reserved. OBJECTIVE To evaluate the impact of early infectious diseases (ID) antimicrobial stewardship (AMS) intervention on inpatient sepsis antibiotic management. DESIGN Interventional, nonrandomized, controlled study. SETTING Tertiary-care referral hospital, Sydney, Australia. PATIENTS Consecutive, adult, non-intensive care unit (non-ICU) inpatients triggering an institutional clinical sepsis pathway from May to August 2015. INTERVENTION All patients reviewed by an ID Fellow within 24 hours of sepsis pathway trigger underwent case review and clinic file documentation of recommendations. Those not reviewed by an ID Fellow were considered controls and received standard sepsis pathway care. The primary outcome was antibiotic appropriateness 48 hours after sepsis trigger. RESULTS In total, 164 patients triggered the sepsis pathway: 6 patients were excluded (previous sepsis trigger); 158 patients were eligible; 106 had ID intervention; and 52 were control cases. Of these 158 patients, 91 (58%) had sepsis, and 15 of these 158 (9.5%) had severe sepsis. Initial antibiotic appropriateness, assessable in 152 of 158 patients, was appropriate in 80 (53%) of these 152 patients and inappropriate in 72 (47%) of these patients. In the intervention arm, 93% of ID Fellow recommendations were followed or partially followed, including 53% of cases in which antibiotics were de-escalated. ID Fellow intervention improved antibiotic appropriateness at 48 hours by 24% (adjusted risk ratio, 1.24; 95% confidence interval, 1.04-1.47; P=.035). The appropriateness agreement among 3 blinded ID staff opinions was 95%. Differences in intervention and control group mortality (13% vs 17%) and median length of stay (13 vs 17.5 days) were not statistically significant. CONCLUSION Sepsis overdiagnosis and delayed antibiotic optimization may reduce sepsis pathway effectiveness. Early ID AMS improved antibiotic management of non-ICU inpatients with suspected sepsis, predominantly by de-escalation. Further studies are needed to evaluate clinical outcomes. Infect Control Hosp Epidemiol 2017;38:1032-1038

Bacterial Antigen Expression Is an Important Component in Inducing an Immune Response to Orally Administered Salmonella-Delivered DNA Vaccines

BACKGROUND: The use of Salmonella to deliver heterologous antigens from DNA vaccines is a well-accepted extension of the success of oral Salmonella vaccines in animal models. Attenuated S. typhimurium and S. typhi strains are safe and efficacious, and their use to deliver DNA vaccines combines the advantages of both vaccine approaches, while complementing the limitations of each technology. An important aspect of the basic biology of the Salmonella/DNA vaccine platform is the relative contributions of prokaryotic and eukaryotic expression in production of the vaccine antigen. Gene expression in DNA vaccines is commonly under the control of the eukaryotic cytomegalovirus (CMV) promoter. The aim of this study was to identify and disable putative bacterial promoters within the CMV promoter and evaluate the immunogenicity of the resulting DNA vaccine delivered orally by S. typhimurium. METHODOLOGY/PRINCIPAL FINDINGS: The results reported here clearly demonstrate the presence of bacterial promoters within the CMV promoter. These promoters have homology to the bacterial consensus sequence and functional activity. To disable prokaryotic expression from the CMV promoter a series of genetic manipulations were performed to remove the two major bacterial promoters and add a bacteria transcription terminator downstream of the CMV promoter. S. typhimurium was used to immunise BALB/c mice orally with a DNA vaccine encoding the C-fragment of tetanus toxin (TT) under control of the original or the modified CMV promoter. Although both promoters functioned equally well in eukaryotic cells, as indicated by equivalent immune responses following intramuscular delivery, only the original CMV promoter was able to induce an anti-TT specific response following oral delivery by S. typhimurium. CONCLUSIONS: These findings suggest that prokaryotic expression of the antigen and co-delivery of this protein by Salmonella are at least partially responsible for the successful oral delivery of C-fragment DNA vaccines containing the CMV promoter by S. typhimurium