Low-temperture electrostatic silicon-to-silicon seals using sputtered borosilicate glass

Silicon members are hermetically sealed to each other. Process produces no measurable deformation of silicon surfaces and is compatible with package designs of tight tolerance. Seals have been made with glass coatings in 10-mm to 20-mm thickness range without any prior annealing of coated silicon substrates

The colour-magnitude relations of ClJ1226.9+3332, a massive cluster of galaxies at z=0.89

(Abridged) The colour-magnitude relations of one of the most massive, high redshift clusters of galaxies known have been studied. Photometry has been measured in the V, R, I, z, F606W, F814W, J and K bands to a depth of K*+2.5 and spectroscopy confirms 27 K band selected cluster members. The V-K colours are equivalent to a rest-frame colour of ~2700A-J, and provide a very sensitive measure of star-formation activity. HST ACS imaging has been used to morphologically classify the galaxies. The cluster has a low early-type fraction compared to nearby clusters, with only 33% of the cluster members having types E or S0. The early-type member galaxies form a clear red-sequence in all colours. The scatter and slope of the relations show no evolution compared to the equivalent Coma cluster relations, suggesting the stellar populations are already very old. The normalisation of the relations has been compared to models based on synthetic stellar populations, and are most consistent with stellar populations forming at z>3. Some late-type galaxies were found to lie on the red-sequence, suggesting that they have very similar stellar populations to the early-types. These results present a picture of a cluster in which the early-type galaxies are all old, but in which there must be future morphological transformation of galaxies to match the early-type fraction of nearby clusters. In order to preserve the tight colour-magnitude relation of early-types seen in nearby clusters, the late-type galaxies must transform their colours, through the cessation of star-formation, before the morphological transformation occurs. Such evolution is observed in the late-types lying on the colour-magnitude relation.Comment: Accepted for publication in MNRAS. 14 pages, 5 figure

Baseline for assessing the impact of fairtrade certification on cocoa farmers and cooperatives in Côte d’Ivoire

Report commissioned by Fairtrade Africa and Fairtrade International. World Agroforestry Centre, Nairobi, Kenya. Farms-Forests-LandscapesIn 2014, Fairtrade International, Fairtrade Africa, the World Agroforestry Centre (ICRAF) and Bioversity International initiated a collaboration for the development of a multidimensional baseline on small-scale cocoa farmers and their cooperatives in West Africa. The baseline is expected to provide a fuller understanding of the current situation for Fairtrade cocoa production and marketing as well as provide the foundation for rigorous assessment of outcomes and impacts of Fairtrade certification on cocoa cooperatives and smallholder households in West Africa in the future. Côte d’Ivoire and Ghana, the two largest Fairtrade cocoa producers in West Africa, provide about 68 percent of the cocoa that is sold under Fairtrade terms in global markets. In 2013, the year this study was commissioned, the volume of Fairtrade cocoa sold from West Africa reached 133 400 tonnes, involving 71 cooperatives and producer associations and 138 800 farmers. Most of this cocoa originated from Côte d’Ivoire and Ghana. The rapid growth in the number of cocoa-producing organizations joining the Fairtrade system in Côte d’Ivoire and Ghana provides a unique opportunity to build a baseline on Fairtrade cocoa producers in West Africa for future monitoring and impact assessment. This report focuses on the Fairtrade cocoa baseline for Côte d’Ivoire (a similar report is available for Ghana). It describes the conceptual framework and methods used in the design of the baseline, followed by an assessment of the context in Côte d’Ivoire. Key features of the baseline data at the cooperative and household levels are covered in detail. The report concludes with some recommendations to Fairtrade for expanding Fairtrade International in Côte d’Ivoire and for follow-up actions for future baseline work

Correlation between X-ray Lightcurve Shape and Radio Arrival Time in the Vela Pulsar

We report the results of simultaneous observations of the Vela pulsar in X-rays and radio from the RXTE satellite and the Mount Pleasant Radio Observatory in Tasmania. We sought correlations between the Vela's X-ray emission and radio arrival times on a pulse by pulse basis. At a confidence level of 99.8% we have found significantly higher flux density in Vela's main X-ray peak during radio pulses that arrived early. This excess flux shifts to the 'trough' following the 2nd X-ray peak during radio pulses that arrive later. Our results suggest that the mechanism producing the radio pulses is intimately connected to the mechanism producing X-rays. Current models using resonant absorption of radio emission in the outer magnetosphere as a cause of the X-ray emission are explored as a possible explanation for the correlation.Comment: 6 pages, 5 figures, accepted by Ap

Toward Understanding the Role of Amot80 Lipid Binding in Cellular Proliferation and Migration

poster abstractsAmots are adaptor proteins which coordinate signaling that controls cellular differentiation and proliferation, and their Amot coiled-coil homology (ACCH) domain is able to bind lipids with specificity which leads to membrane deformation and targets transcription factors to the nucleus. Understanding the biophysical mechanisms involved in lipid binding may provide pathways to modulate protein sorting and downstream signaling events inducing cellular differentiation, cancer cell proliferation, and migration. At this time, all work reported on signaling based on Amot expression is unable to distinguish between the role of the Amot80 and the 130 family members as they share a common ACCH domain. The goal of this project is to specifically relate the Amot80 ACCH lipid binding with function related to cancer phenotypes Mutations were carried forward based on lipid sedimentation, FRET, and SAXS assays against the ACCH domain of the protein. Site-directed mutagenesis was then employed to probe the specific contributions of 7 selected lysines and arginines toward lipid head-group binding in the full length protein. The polarity/scaffolding signaling effect of mutations in the Amot80 will be monitored by matrigel, accumulation/cell counting, and titrated thymidine incorporation assays. Cell morphology will be imaged by confocal imaging, and cellular migration will be recorded by video. The effects on YAP1/2 and MAPK activation will be assessed by immunoblot analysis. The changes will then be correlated in extracellular scaffolding and migration with immunoblots and cellular staining. Likewise, effects on proliferation will be monitored by MTT assays. The hypothesis of this aim is that modulation of Amot’s ability to bind selective lipids will interrupt the signaling pathways leading to cellular migration, differentiation, and proliferation. This work was supported by the IUPUI Undergraduate Research Opportunities Program (UROP) and NIH K01CA169078-01

Mipomersen preferentially reduces small low-density lipoprotein particle number in patients with hypercholesterolemia

AbstractBackgroundBecause of variability in lipoprotein cholesterol content, low-density lipoprotein (LDL) cholesterol frequently underrepresents or overrepresents the number of LDL particles. Mipomersen is an antisense oligonucleotide that reduces hepatic production of apolipoprotein B–100, the sole apolipoprotein of LDL.ObjectiveTo characterize the effects of mipomersen on lipoprotein particle numbers as well as subclass distribution using nuclear magnetic resonance (NMR) spectroscopy.MethodsWe compared the tertiary results for the direct measurement of LDL particle numbers by NMR among 4 placebo-controlled, phase 3 studies of mipomersen that had similar study designs but different patient populations: homozygous familial hypercholesterolemia (HoFH), severe hypercholesterolemia, heterozygous familial hypercholesterolemia with established coronary artery disease, or hypercholesterolemia with high risk for coronary heart disease (HC-CHD).ResultsHoFH patients had the highest median total LDL particles at baseline compared with HC-CHD patients, who had the lowest. At baseline, the HoFH population uniquely had a greater mean percentage of large LDL particles (placebo, 60.2%; mipomersen, 54.9%) compared with small LDL particles (placebo, 33.1%; mipomersen, 38.9%). In all 4 studies, mipomersen was associated with greater reductions from baseline in the concentrations of small LDL particles compared with those of large LDL particles, and both total LDL particles and small LDL particles were statistically significantly reduced.ConclusionsMipomersen consistently reduced all LDL particle numbers and preferentially reduced the concentration of small LDL particles in all 4 phase 3 studies

HOPE: Help fOr People with money, employment, benefit or housing problems: study protocol for a randomised controlled trial

Background: Self-harm and suicide increase in times of economic recession. Factors including job loss, austerity measures, financial difficulties and house repossession contribute to the risk. Vulnerable individuals commonly experience difficulties in navigating the benefits system and in accessing the available sources of welfare and debt advice, and this contributes to their distress. Our aim is to determine the feasibility and acceptability of a brief psychosocial intervention (the “HOPE” service) for people presenting to hospital emergency departments (ED) following self-harm or in acute distress because of financial, employment, or welfare (benefit) difficulties. Method: A pilot study including randomisation will be employed to determine whether it is possible to undertake a full-scale trial. Twenty people presenting to the ED who have self-harmed, have suicidal thoughts and depression and/or are in crisis and where financial, employment or benefit problems are cited as contributory factors will be asked to consent to random allocation to the intervention or control arm on a 2:1 basis. People who require secondary mental health follow-up will be excluded. Those randomised to the intervention arm will receive up to six sessions with a mental health worker who will provide practical help with financial and other problems. The mental health worker will use the motivational interviewing method in their interactions with participants. Control participants will receive one session signposting them to existing relevant support organisations. Participants will be followed up after 3 months. Participants and the mental health workers will take part in qualitative interviews to enable refinement of the intervention. The acceptability of outcome measures including the PHQ-9, GAD-7, repeat self-harm, EQ5D-5L and questions about debt, employment and welfare benefits will be explored. Discussion: This study will assess whether a full-scale randomised trial of this novel intervention to prevent self-harm among those distressed because of financial difficulties is feasible, including the acceptability of randomisation, potential rate of recruitment and the acceptability of outcome measures. Trial registration: ISRCTN5853124