Using foraminifera in STEMSEAS Site 1 to understand the recent paleoceanographic and paleoclimatic history of Tanner Basin, California Borderland

In May of 2016, the STEMSEAS Educational Transit cruise OC1605-tranA collected the STEMSEAS Site 1 core from the Tanner Basin in the California Borderland. This research serves as the first formal survey of the foraminifera preserved within that core. The purpose of this research is to use foraminifera preserved within that core to understand the recent depositional and paleoenvironmental conditions at Site 1, and to place that information into a regional paleoceanographic and paleoclimatic context. In pursuing this purpose, this research aims to answer three questions: 1) Can biostratigraphic markers in the foraminiferal assemblages in STEMSEAS Site 1 core be used to test the hypothesis that a shift in elemental ratio concentrations at 120 cmbsf marks the Pleistocene/Holocene boundary? 2) Is there evidence of turbidity flow deposition at STEMSEAS Site 1? 3) Can the foraminiferal assemblages within STEMSEAS Site 1 core be used to study paleoenvironmental changes in the California Borderland through time? Thirty-four samples of the \u3e63 mm size sediment fraction from the core were analyzed throughout this study, and the data from those samples was compared with lithologic and elemental data collected by the STEMSEAS cruise shipboard party and with regional data. Additionally, radiocarbon dates were obtained to develop an age model for the core, which allowed cored data to be interpreted in a temporal context. In answering the proposed questions, the study found that: 1) The shift in elemental data at 120 cmbsf occurs very close to (~1 kyr following) the Pleistocene/Holocene boundary, and the Pleistocene/Holocene transition represents a period of low dissolved oxygen supply within the Tanner Basin and low surface productivity. 2) A small percentage of benthic foraminifera present at Site 1 were displaced to Site 1 from a shallower depth, suggesting that turbidity flows did impact sedimentation at this location, but were not the dominant sediment transport process. 3) The core records a paleoenvironmental history of semi-regular millennial scale variation in sea surface temperature, upwelling strength, and nutrient influx that may be driven by oscillating and increasing frequency of El Niño/Southern Oscillation events

Non-invasive anaerobic threshold measurement using fuzzy model interpolation

The interface between skeletal muscle activation through aerobic and anaerobic glycolysis is of key interest to sportspeople and athletes who participate in medium to long distance sports, such as middle- and long-distance running, cycling, swimming, rowing, kayaking and a variety of other events. To date, the gold standard for measuring anaerobic threshold (AT) is a structured test to exhaustion where blood lactate concentration is measured at regular intervals. However, the need for invasive testing, requiring trained personnel and specialist equipment, limits the availability of such tests. This paper proposes a non-invasive AT measurement method, which validates well against AT measured using lactate analysis. In addition, the proposed test has a relatively loose set of requirements on the exercise test protocol required and just requires a measure of exercise intensity and heart-rate. While the test is applicable to a range of sports, usage is demonstrated in this paper for a set of cyclists, using velocity as a measure of exercise intensity

Two-dimensional imaging of edge-localized modes in KSTAR plasmas unperturbed and perturbed by n=1 external magnetic fields

The temporal evolution of edge-localized modes (ELMs) has been studied using a 2-D electron cyclotron emission imaging system in the KSTAR tokamak. The ELMs are observed to evolve in three distinctive stages: the initial linear growth of multiple filamentary structures having a net poloidal rotation, the interim state of regularly spaced saturated filaments, and the final crash through a short transient phase characterized by abrupt changes in the relative amplitudes and distance among filaments. The crash phase, typically consisted of multiple bursts of a single filament, involves a complex dynamics, poloidal elongation of the bursting filament, development of a fingerlike bulge, and fast localized burst through the finger. Substantial alterations of the ELM dynamics, such as mode number, poloidal rotation, and crash time scale, have been observed under external magnetic perturbations with the toroidal mode number n = 1. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3694842]X1125sciescopu

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.

Cellular stress responses are frequently governed by the subcellular localization of critical effector proteins. Apoptosis-inducing Factor (AIF) or Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), for example, can translocate from mitochondria to the nucleus, where they modulate apoptotic death pathways. Hypoxia-inducible gene domain 1A (HIGD1A) is a mitochondrial protein regulated by Hypoxia-inducible Factor-1α (HIF1α). Here we show that while HIGD1A resides in mitochondria during physiological hypoxia, severe metabolic stress, such as glucose starvation coupled with hypoxia, in addition to DNA damage induced by etoposide, triggers its nuclear accumulation. We show that nuclear localization of HIGD1A overlaps with that of AIF, and is dependent on the presence of BAX and BAK. Furthermore, we show that AIF and HIGD1A physically interact. Additionally, we demonstrate that nuclear HIGD1A is a potential marker of metabolic stress in vivo, frequently observed in diverse pathological states such as myocardial infarction, hypoxic-ischemic encephalopathy (HIE), and different types of cancer. In summary, we demonstrate a novel nuclear localization of HIGD1A that is commonly observed in human disease processes in vivo

Mass spectrometry of B. subtilis CopZ: Cu(I)-binding and interactions with bacillithiol

CopZ from Bacillus subtilis is a well-studied member of the highly conserved family of Atx1-like copper chaperones. It was previously shown via solution and crystallographic studies to undergo Cu(I)-mediated dimerisation, where the CopZ dimer can bind between one and four Cu(I) ions. However, these studies could not provide information about the changing distribution of species at increasing Cu(I) levels. To address this, electrospray ionisation mass spectrometry using soft ionisation was applied to CopZ under native conditions. Data revealed folded, monomeric CopZ in apo- and Cu(I)-bound forms, along with Cu(I)-bound dimeric forms of CopZ at higher Cu(I) loading. Cu4(CopZ)2 was the major dimeric species at loadings >1 Cu(I)/CopZ, indicating the cooperative formation of the tetranuclear Cu(I)-bound species. As the principal low molecular weight thiol in B. subtilis, bacillithiol (BSH) may play a role in copper homeostasis. Mass spectrometry showed that increasing BSH led to a reduction in Cu(I)-bound dimeric forms, and the formation of S-bacillithiolated apo-CopZ and BSH adducts of Cu(I)-bound forms of CopZ, where BSH likely acts as a Cu(I) ligand. These data, along with the high affinity of BSH for Cu(I), determined here to be β2(BSH) = ∼4 × 1017 M−2, are consistent with a role for BSH alongside CopZ in buffering cellular Cu(I) levels. Here, mass spectrometry provides a high resolution overview of CopZ–Cu(I) speciation that cannot be obtained from less discriminating solution-phase methods, thus illustrating the potential for the wider application of this technique to studies of metal–protein interactions

Conduit dynamics and post-explosion degassing on Stromboli:a combined UV camera and 1 numerical modelling treatment

Recent gas flux measurements have shown that strombolian explosions are often followed by periods of elevated flux, or ‘gas codas’, with durations of order a minute. Here, we present UV camera data from 200 events recorded at Stromboli volcano to constrain the nature of these codas for the first time, providing estimates for combined explosion plus coda SO2 masses of ≈ 18 – 225 kg. Numerical simulations of gas slug ascent show that substantial proportions of the initial gas mass can be distributed into a train of ‘daughter bubbles’ released from the base of the slug, which we suggest, generate the codas, on bursting at the surface. This process could also cause transitioning of slugs into cap bubbles, significantly reducing explosivity. This study is the first attempt to combine high temporal resolution gas flux data with numerical simulations of conduit gas flow to investigate volcanic degassing dynamics

Wigs, disguises and child's play : solidarity in teacher education

It is generally acknowledged that much contemporary education takes place within a dominant audit culture, in which accountability becomes a powerful driver of educational practices. In this culture both pupils and teachers risk being configured as a means to an assessment and target-driven end: pupils are schooled within a particular paradigm of education. The article discusses some ethical issues raised by such schooling, particularly the tensions arising for teachers, and by implication, teacher educators who prepare and support teachers for work in situations where vocational aims and beliefs may be in in conflict with instrumentalist aims. The article offers De Certeau’s concept of ‘la perruque’ to suggest an opening to playful engagement for human ends in education, as a way of contending with and managing the tensions generated. I use the concept to recover a concept of solidarity for teacher educators and teachers to enable ethical teaching in difficult times