A non-destructive method for distinguishing reindeer antler (Rangifer tarandus) from red deer antler (Cervus elaphus) using X-Ray micro-tomography coupled with SVM classifiers

International audienc

Auger et al F2 2D4D vs AGD input file

2D to 4D digit ratio measured by semi-automatic image analysis vs relative anogenital distance (AGD) measured by a standardized method according to exposure conditions in the F2 unexposed generation sired by exposed fathers and unexposed mother

Data from: Environmental levels of oestrogenic and antiandrogenic compounds feminize digit ratios in male rats and their unexposed male progeny

Digit length ratios, especially the second-to-fourth digit ratio (2D : 4D), are associated with various pathological and behavioural conditions in many species including humans and are dependent upon prenatal androgen to oestrogen balance. It is unknown whether digit ratios are modified by environmental exposure to ubiquitous endocrine disruptors. We studied the effect on adult male Wistar rat digit ratios of a gestational exposure to the oestrogenic and antiandrogenic compounds bisphenol A (BPA), genistein and vinclozolin, in low doses, and in combination with investigating in parallel a possible sexual dimorphism of this trait. We also investigated the effects on the male progeny not exposed during gestation. X-rays were taken of the left and right forepaws, and 2D–5D proximal to distal phalanx distances were measured by a standardized procedure based on semi-automatic image analysis. We provide evidence that there is a sexual dimorphism of digit ratios in the Wistar rat, and we found that BPA alone or in combination with genistein and vinclozolin significantly feminized digit ratios in male rats. Intriguingly, significant feminization of digit ratios was also found in the unexposed male progeny of males that had been exposed to compound mixtures. In conclusion, prenatal environmental levels of endocrine-active substances permanently disrupt digit ratios. Digit ratio measurement in adults is thus a promising biomarker of prenatal exposure to low-dose endocrine disruptors in rodents, with potential implications for future studies in humans

Environmental levels of bisphenol A, genistein and vinclozolin feminize digit length ratios in male rats: towards a new sensitive indicator of prenatal endocrine disruption and its impact in the progeny. – CIME project

Environmental levels of bisphenol A, genistein and vinclozolin feminize digit length ratios in male rats: towards a new sensitive indicator of prenatal endocrine disruption and its impact in the progeny. – CIME project. Colloque PNR-P

Grape seed extracts inhibit dentin matrix degradation by MMP-3

International audienc

Mean and standard deviation of the biometric measurements and inter-group comparisons (Wilcoxon test) for the archaeological corpus.

<p>It included 50 antler artefacts of which 23 came from red deer and 27 from reindeer (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149658#pone.0149658.s005" target="_blank">S3 Table</a>).</p

Anatomical location of the antler samples (modern corpus).

<p>Anatomical location of the antler samples (modern corpus).</p

Mean and standard deviation of the biometric measurements and inter-group comparisons (Wilcoxon test) for the modern corpus.

<p>It included 39 antler samples of which 17 were red deer samples and 22 were reindeer samples (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149658#pone.0149658.s003" target="_blank">S1 Table</a>).</p

Auger et al F2 digit length and ratio input file

All basic measurements on digit length and ratio in the progeny according to exposures conditions in fathers; from semi automatic image analysis of forepaw X-rays in the second unexposed generation sired by exposed fathers and unexposed mothers

Confusion matrix from the SVM classification (predictive statistics) obtained by cross-validation in which the training dataset was the modern corpus and the validation dataset was the archaeological corpus.

<p>The decision rule for modern material could not be transferred to the archaeological material (accuracy: 42%).</p