Different Levels of DNA Methylation Detected in Human Sperms after Morphological Selection Using High Magnification Microscopy

Objective. To analyze DNA methylation levels between two groups of spermatozoa taken from the same sample, following morphological selection by high magnification (HM) at 6100x microscopy. A prospective study was conducted and studied 876 spermatozoa from 10 randomly selected men. Sperm morphology was characterized at HM according to criteria previously established. High-scoring Score 6 and low-scoring Score 0 sperm were selected. Sperm DNA methylation level was assessed using an immunoassay method targeting 5-methylcytosine residues by fluorescence microscopy with imaging analysis system to detect DNA methylation in single spermatozoon. Results. In total, 448 S6 spermatozoa and 428 S0 spermatozoa were analyzed. A strong relationship was found between sperm DNA methylation levels and sperm morphology observed at HM. Sperm DNA methylation level in the S6 group was significantly lower compared with that in the S0 group (p<10-6), OR = 2.4; and p<0.001, as determined using the Wilcoxon test. Conclusion. Differences in DNA methylation levels are associated with sperm morphology variations as observed at HM, which allows spermatozoa with abnormal levels to be discarded and ultimately decrease birth defects, malformations, and epigenetic diseases that may be transmitted from sperm to offspring in ICSI

Probing the structure and evolution of active galactic nuclei with the ultraviolet polarimeter POLLUX aboard LUVOIR

The ultraviolet (UV) polarization spectrum of nearby active galactic nuclei (AGN) is poorly known. The Wisconsin Ultraviolet Photo-Polarimeter Experiment and a handful of instruments on board the Hubble Space Telescope were able to probe the near- and mid-UV polarization of nearby AGN, but the far-UV band (from 1200 Å down to the Lyman limit at 912 Å) remains completely uncharted. In addition, the linewidth resolution of previous observations was at best 1.89 Å. Such a resolution is not sufficient to probe in detail quantum mechanical effects, synchrotron and cyclotron processes, scattering by electrons and dust grains, and dichroic extinction by asymmetric dust grains. Exploring those physical processes would require a new, high-resolution, broadband polarimeter with full ultraviolet-band coverage. In this context, we discuss the AGN science case for POLLUX, a high-resolution UV spectropolarimeter, proposed for the 15-meter primary mirror option of LUVOIR (a multi-wavelength space observatory concept being developed by the Goddard Space Flight Center and proposed for the 2020 Decadal Survey Concept Study)

Molecular Profiling of Spermatozoa Reveals Correlations between Morphology and Gene Expression: A Novel Biomarker Panel for Male Infertility

International audienceChoosing spermatozoa with an optimum fertilizing potential is one of the major challenges in assisted reproductive technologies (ART). This selection is mainly based on semen parameters, but the addition of molecular approaches could allow a more functional evaluation. To this aim, we used sixteen fresh sperm samples from patients undergoing ART for male infertility and classified them in the high-and poor-quality groups, on the basis of their morphology at high magnification. Then, using a DNA sequencing method, we analyzed the spermatozoa methylome to identify genes that were differentially methylated. By Gene Ontology and protein-protein interaction network analyses, we defined candidate genes mainly implicated in cell motility, calcium reabsorption, and signaling pathways as well as transmembrane transport. RT-qPCR of high-and poorquality sperm samples allowed showing that the expression of some genes, such as AURKA, HDAC4, CFAP46, SPATA18, CACNA1C, CACNA1H, CARHSP1, CCDC60, DNAH2, and CDC88B, have different expression levels according to sperm morphology. In conclusion, the present study shows a strong correlation between morphology and gene expression in the spermatozoa and provides a biomarker panel for sperm analysis during ART and a new tool to explore male infertility

Abundance analysis of targets for the COROT/MONS asteroseismology missions. II. Abundance analysis of the COROT main targets

14 pages, 6 figures, accepted 11th of June 2004 by A&AInternational audienceOne of the goals of the ground-based support program for the COROT and MONS/RÖMER satellite missions is to characterize suitable target stars for the part of the missions dedicated to asteroseismology. We present the detailed abundance analysis of nine of the potential COROT main targets using the semi-automatic software VWA. For two additional COROT targets we could not perform the analysis due to the high rotational velocity of these stars. For five stars with low rotational velocity we have also performed abundance analysis by a classical equivalent width method in order to test the reliability of the VWA software. The agreement between the different methods is good. We find that it is necessary to measure abundances extracted from each line relative to the abundances found from a spectrum of the Sun in order to remove systematic errors. We have constrained the global atmospheric parameters Teff, log g, and [Fe/H] to within 70-100 K, 0.1-0.2 dex, and 0.1 dex for five stars which are slow rotators (v sin i -1). For most of the stars we find good agreement with the parameters found from line depth ratios, H alpha lines, Strömgren indices, previous spectroscopic studies, and also log g determined from the HIPPARCOS parallaxes. For the fast rotators (v sin i > 60 km s-1) it is not possible to constrain the atmospheric parameters

Comment prendre en compte la dimension éco-responsable des produits de santé tout au long de leur cycle de vie?

National audienceL’achat de produits santé représente environ 50 % des émissions gaz à effet serre du système français. Cet enseignement tiré la publication travaux Shift Project en novembre 2021 a été un élément déclencheur au sein santé, accélérant les réflexions et mises œuvre d’actions visant réduire impacts environnementaux avant, pendant après soins. Au-delà l’empreinte carbone, sont divers : eau, air, sols…et ce tout long vie produits. Nous avons choisi diviser cycle quatre étapes principales recherche développement production, distribution l’accès marché, l’usage enfin gestion fin vie. L’analyse cadre règlementaire chaque étape d’initiatives existantes, décrites dans littérature ou par acteurs terrain ont structuré nourri notre réflexion. constaté que règlementation existante conduit une lecture exclusivement centrée sur le risque sanitaire, avec peu pas prise compte environnemental, qui constitue pourtant lui-même pour santé. Par ailleurs, mise certaines actions structurantes cours 3 premières permettrait simplifier, voire supprimer problématique aujourd’hui majeure déchets liée C’est sens nous produisons 9 recommandations permettant meilleur

How can the environmental sustainability of healthcare products be taken into account throughout their life cycle?

National audienceHealthcare product procurement accounts for around 50% of the French healthcare system’s greenhouse gas emissions. This lesson learned from the publication of the Shift Project’s work in November 2021 has been a catalyst within the healthcare system, accelerating the consideration and implementation of actions aimed at reducing the environmental impact of the healthcare system, before, during and after care. In addition to their carbon footprint, healthcare products have a wide range of environmental impacts, including on water, air and soil, throughout their entire life cycle. We have chosen to divide this life cycle into four main stages: from research and development to production, distribution and market access, use and finally end-of-life management. Analysis of the regulatory framework at each stage and of existing initiatives described in the literature or by those in the field have structured and fuelled our thinking. We found that existing regulations focus exclusively on the health risk, with little or no consideration of the environmental risk, which is in itself a health risk. Furthermore, the implementation of certain structuring actions during the first 3 stages of the life cycle would make it possible to simplify or even eliminate the major problem of waste management associated with the end-of-life of healthcare products. With this in mind, we have produced 9 recommendations to ensure that the environmental impact of healthcare products is better taken into account throughout their life cycle

Placental restriction increases adipose leptin gene expression and plasma leptin and alters their relationship to feeding activity in the young lamb

Low birth weight and catch-up growth predict increased adiposity in children and adults. This may be due in part to leptin resistance, as adults who were born small exhibit increased plasma leptin concentration relative to adiposity. Placental restriction (PR), a major cause of intrauterine growth restriction, reduces size at birth and increases feeding activity and adiposity by 6 wk in sheep. We hypothesized that PR would increase plasma leptin concentration and alter its relationship with feeding activity and adiposity in young lambs. Body size, plasma leptin, feeding activity, adiposity, leptin, and leptin receptor gene expression in adipose tissue were measured (12 control, 12 PR). PR reduced size at birth and increased adiposity. Plasma leptin concentration decreased with age, but to a lesser extent after PR and correlated positively with adiposity similarly in control and PR. PR increased plasma leptin concentration and perirenal adipose tissue leptin expression. Feeding activity correlated negatively with plasma leptin concentration in controls, but positively after PR. PR increases adipose tissue leptin expression and plasma leptin concentration, however, this increased abundance of peripheral leptin does not inhibit feeding activity (suckling event frequency), suggesting PR programs resistance to appetite and energy balance regulation by leptin, leading to early onset obesity.Miles J. De Blasio, Dominique Blache, Kathryn L. Gatford, Jeffrey S. Robinson and Julie A. Owen