Museus, Educação e Multiculturalismo: Um Estudo De Caso

A presente dissertação pretende contribuir para a análise das temáticas relacionadas com os museus, educação e multiculturalismo. A partir de duas iniciativas promovidas pela Fundação Calouste Gulbenkian: a exposição Looking Both Ways. Das Esquinas do Olhar. Arte da Diáspora Africana Contemporânea, em 2005 e Fórum Cultural O Estado do Mundo - Plataforma 2, em 2006/2007. No que toca à última iniciativa focamo-nos no programa Transfert e no programa Jardim do Mundo. Partindo da análise deste caso prático, foi nosso objectivo compreender de que forma os museus abordam a questão do multiculturalismo. A exposição Looking Both Ways. Das Esquinas do Olhar. Arte da Diáspora Africana Contemporânea focou trabalhos de artistas africanos onde se evidenciavam as influências pessoais de cada um. O projecto ARTAFRICA desempenhou aqui um papel fundamental. O Fórum Cultural O Estado do Mundo - Plataforma 2 promoveu por um lado a fruição de objectos de arte noutros espaços que não os museus – Tranfert; por outro, desenvolveu uma iniciativa integrando elementos de grupos minoritários – Jardim do Mundo. Adicionalmente, foi nosso objectivo reflectir sobre a pertinência de um projecto educativo neste género de iniciativas centrando-nos na figura central do educador enquanto mediador. Analisamos também os termos multiculturalismo e interculturalismo dentro do contexto museológico. Por fim, concluímos que os museus do século XXI que incluem na sua programação as temáticas do multiculturalismo parecem tender para centros culturaisInstituto Superior de Ciências do Trabalho e da Empres

Bichectomia: bola de Bichat em foco

A cirurgia de bichectomia consiste na remoção do Corpo Adiposo da Bochecha e tem tido sucesso no âmbito das intervenções cirúrgicas orais, contribuindo para a melhoria estética da face, para a harmonia do sorriso e tem sido ainda utilizada com o intuito de reconstruir defeitos maxilares, defeitos no palato duro e mole, rebordo alveolar, bochecha e fossa tonsilar/amigdalina. O presente estudo objetivo explorar o tema bichectomia: abordam-se aspetos anatómicos relacionados com a intervenção cirúrgica, descrevem-se os seus objetivos, os seus contextos de aplicação e discutem-se as suas vantagens e desvantagens. Foi realizada uma pesquisa bibliográfica (2008 e 2018), nos motores de busca PubMed, B-on e Google Académico com as palavras chave: “bichectomy”; “bichat´s fat pad”; “adipose tissue”; “anatomia da face”; “estética facial” e “corpo adiposo da bochecha.” Utilizada em contexto estético, a bichectomia tem sido ainda utilizada no tratamento da comunicação oroantral, da osteonecrose dos maxilares, de cistos e tumores da cavidade oral, da fibrose submucosa oral e em casos clínicos de peri-implantite. Conclui-se que a bichectomia se apresenta como um recurso terapêutico de interesse crescente na área cirúrgica de cabeça e pescoço.The bichectomy surgery consists of the removal of the Adipose Body of the Cheek and has been successful in the scope of the oral surgical interventions, contributing to the aesthetic improvement of the face, to the harmony of the smile and has still been used in order to reconstruct maxillary defects, defects on the hard and soft palate, alveolar ridge, cheek and tonsillar fossa. The present study aims to explore the bichectomy theme: anatomical aspects related to surgical intervention are described, their objectives are described, their contexts of application and their advantages and disadvantages are discussed. A bibliographic search was carried out (2008 and 2018), in the search engines PubMed, B-on and Google Scholar with the key words: “bichectomy”; “Bichat's fat pad”; Adipose tissue; “Face anatomy”; “Facial aesthetics” and “cheek fat body”. Used in an aesthetic context, bichectomy has also been used in the treatment of oroantral communication, maxillary osteonecrosis, oral cavity cysts and tumors, oral submucosal fibrosis and clinical cases of peri-implantitis. It is concluded that bichectomy presents as a therapeutic resource of increasing interest in the surgical area of head and neck

Bichectomia: bola de Bichat em foco

A cirurgia de bichectomia consiste na remoção do Corpo Adiposo da Bochecha e tem tido sucesso no âmbito das intervenções cirúrgicas orais, contribuindo para a melhoria estética da face, para a harmonia do sorriso e tem sido ainda utilizada com o intuito de reconstruir defeitos maxilares, defeitos no palato duro e mole, rebordo alveolar, bochecha e fossa tonsilar/amigdalina. O presente estudo objetivo explorar o tema bichectomia: abordam-se aspetos anatómicos relacionados com a intervenção cirúrgica, descrevem-se os seus objetivos, os seus contextos de aplicação e discutem-se as suas vantagens e desvantagens. Foi realizada uma pesquisa bibliográfica (2008 e 2018), nos motores de busca PubMed, B-on e Google Académico com as palavras chave: “bichectomy”; “bichat´s fat pad”; “adipose tissue”; “anatomia da face”; “estética facial” e “corpo adiposo da bochecha.” Utilizada em contexto estético, a bichectomia tem sido ainda utilizada no tratamento da comunicação oroantral, da osteonecrose dos maxilares, de cistos e tumores da cavidade oral, da fibrose submucosa oral e em casos clínicos de peri-implantite. Conclui-se que a bichectomia se apresenta como um recurso terapêutico de interesse crescente na área cirúrgica de cabeça e pescoço.The bichectomy surgery consists of the removal of the Adipose Body of the Cheek and has been successful in the scope of the oral surgical interventions, contributing to the aesthetic improvement of the face, to the harmony of the smile and has still been used in order to reconstruct maxillary defects, defects on the hard and soft palate, alveolar ridge, cheek and tonsillar fossa. The present study aims to explore the bichectomy theme: anatomical aspects related to surgical intervention are described, their objectives are described, their contexts of application and their advantages and disadvantages are discussed. A bibliographic search was carried out (2008 and 2018), in the search engines PubMed, B-on and Google Scholar with the key words: “bichectomy”; “Bichat's fat pad”; Adipose tissue; “Face anatomy”; “Facial aesthetics” and “cheek fat body”. Used in an aesthetic context, bichectomy has also been used in the treatment of oroantral communication, maxillary osteonecrosis, oral cavity cysts and tumors, oral submucosal fibrosis and clinical cases of peri-implantitis. It is concluded that bichectomy presents as a therapeutic resource of increasing interest in the surgical area of head and neck

Optimization strategies for metabolic networks

<p>Abstract</p> <p>Background</p> <p>The increasing availability of models and data for metabolic networks poses new challenges in what concerns optimization for biological systems. Due to the high level of complexity and uncertainty associated to these networks the suggested models often lack detail and liability, required to determine the proper optimization strategies. A possible approach to overcome this limitation is the combination of both kinetic and stoichiometric models. In this paper three control optimization methods, with different levels of complexity and assuming various degrees of process information, are presented and their results compared using a prototype network.</p> <p>Results</p> <p>The results obtained show that Bi-Level optimization lead to a good approximation of the optimum attainable with the full information on the original network. Furthermore, using Pontryagin's Maximum Principle it is shown that the optimal control for the network in question, can only assume values on the extremes of the interval of its possible values.</p> <p>Conclusions</p> <p>It is shown that, for a class of networks in which the product that favors cell growth competes with the desired product yield, the optimal control that explores this trade-off assumes only extreme values. The proposed Bi-Level optimization led to a good approximation of the original network, allowing to overcome the limitation on the available information, often present in metabolic network models. Although the prototype network considered, it is stressed that the results obtained concern methods, and provide guidelines that are valid in a wider context.</p

Thymus citriodorus: phenolic characterization and antioxidant activity

The present work aims to determine the phenolic composition of Thymus citriodorus and to estimate its antioxidant capacity. An ethanolic extract was prepared from the plant and its total phenolic and flavone contents were determined. The phenolic extract was fractionated by reversed-phase HPLC and the major phenolic compounds of each fraction were identified by ESI-MS and MSn analysis, in order to establish the specific phenolic profile of the plant. The total phenolic compounds in the ethanolic extract of Thymus citriodorus accounted for 138,75±13,56 mg/g, and its content of flavones was estimated as 27,30±2,15 mg/g. Moreover, the interpretation of the fragmentation pathways under ESI-MS/MS of the collected HPLC fractions allowed concluding that the major phenolic compounds in the plant included some glycoside derivatives of luteonin, naringenin and eriodictyol. Also, the phenolic extract showed a considerable antioxidant activity, as evaluated by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging potential and the reducing potential, suggesting that Thymus citriodorus can be an important source of natural antioxidants

Relatório de estágio profissional

No âmbito das Unidades Curriculares de Estágio Profissional I, II e III, a elaboração do Relatório de Estágio Profissional é um dos pré-requisitos para a sua conclusão com vista à obtenção do grau de mestre em Educação Pré-Escolar e Ensino do 1.º Ciclo do Ensino Básico. O Estágio Profissional teve início a 12 de outubro de 2010 e terminou a 10 de fevereiro de 2012. Foi organizado da seguinte forma: numa primeira etapa, no Jardim Escola João de Deus de Albarraque, de 12 de outubro a 25 de fevereiro de 2011, passando por todos os grupos etários do Pré-Escolar. Numa segunda etapa, o estágio realizou-se no Jardim Escola João de Deus da Estrela, com início a 14 de março de 2011, e terminou a 10 de fevereiro de 2012, passando por todos os grupos etários do 1.º Ciclo do Ensino Básico, sendo este sempre às segundas, terças e sextas feiras, das 9horas às 13horas

Mentha aquatica: source of flavanone glycosides

Mentha aquatica L., commonly known as water mint, is a perennial herb that grows in Europe temperate regions. This plant is used in traditional medicine for the treatment of external inflammation, rheumatism, colds, respiratory problems and difficult menstruation [1]. It has been described as a good source of phenolic compounds with high scavenger activity [2]. In this study, a purified ethanolic extract of M. aquatica was prepared and its specific phenolic composition was determined. The extract of the aerial parts of M. aquatica was prepared according the procedure described by Pereira et al [3] and analyzed by high performance liquid chromatography with diode array detection with quantification of main phenolic compounds, using the external standard method. In order to determine the exact structure of phenolic compounds, the HPLC eluted fractions were manually collected and further analyzed by tandem electrospray mass spectrometry. The purified ethanolic extract of M. aquatica was mainly rich in flavanones comprises eriodictyol, hesperitin and naringenin glycosides. Moreover, the purified ethanolic extract of M. aquatica contained moderate amounts of rosmarinic acid, a phenolic acid very common in Mentha species and in Lamiaceae family

Cloning and expression of a recombinant human CBD (carbohydrate binding domain) : a comparison between two expression models

Genetic engineering is a method that provides the production of recombinant proteins for different purposes. Nowadays there are several and commercially available expression systems aiming the production of these proteins. However, in same cases, the solubility and stability of the produced protein can be a problem, especially for eukaryotic proteins, which need post translational modifications to be biologically active. In the present work, different strategies were tested to increase the solubility of a human carbohydrate binding domain (CBD), present in laforin phosphatase. Namely, different expression systems, different hosts and fermentation conditions, the presence of additives and detergents during lyses, were used. The DNA coding sequence was cloned by PCR into three prokaryotic expression systems: pET 29a, pET 25b; and two eukaryotic systems: pGAPZaC and pPICZaC. The CBD was expressed at high level in pET system. In pET29a the CBD protein was obtained in inclusion bodies. In pET25b, a small amount of soluble protein was obtained in the presence of arginine and CHAPS, in the lyses buffer. Although a soluble recombinant protein was obtained, it was not stable in solution, aggregating easily. On the other hand, the utilisation of two expression systems of Pichia pastoris led to the production of soluble and stable CBD in extra cellular medium; however, this CBD was obtained at low expression level and its activity was not confirmed

Improving the solubility of a recombinant human CBD (carbohydrate binding domain)

Different expression systems have been developed, and are commercially available, aiming at producing recombinant proteins from different organisms, ranging from bacteria to man. However, in same cases, the solubility and stability of the produced protein can be a problem, especially for eukaryotic proteins, which need post translational modifications to be biologically active. In the present work, different strategies were tested to increase the solubility of a human carbohydrate binding domain (CBD), present in laforin phosphatase. Namely, different expression systems, different hosts and fermentation conditions, the presence of additives and detergents during lyses, were used. The DNA coding sequence was cloned by PCR into three prokaryotic expression systems: pET 29a, pET 25b; and two eukaryotic systems: pGAPZaC and pPICZaC. The CBD was expressed at high level in pET system. In pET29a the CBD protein was obtained in inclusion bodies. In pET25b, a small amount of soluble protein was obtained in the presence of 0,6M of arginine, in the lyses buffer. Although a functional recombinant protein was obtained, it was not stable in solution, aggregating easily. On the other hand, the utilisation of two expression systems of Pichia pastoris led to the production of soluble and stable CBD in extra cellular medium; however, this CBD was obtained at low expression level and its activity was not confirmed. Glycosilation of the expressed protein may explain the increased stability, at the expense of reduced functionality. Studies are underway to confirm this hypothesis