Primary Evaluation on Growth Performances of Stress Negative Piétrain Pigs Raised in Hai Phong Province of Vietnam

peer reviewedThe present study was carried out on 19 stress negative Piétrain pigs (Pietrain ReHal), consisting of 13 gilts and 6 young boars imported from Belgium, raised in the livestock farm of Dong Hiep (Hai Phong) in order to evaluate growth performances and their adaptability in the North of Vietnam. Results showed that the average body weight of the whole herd at 2, 4, 5.5, and 8.5 months old was 19.05, 51.05, 85.82, and 119.47 kg, respectively. During the growing periods, except the first stage, the male grew faster than the female and the pigs of the CT genotype grew faster than those of CC genotype although the difference was not significant (P>0.05). The average daily gain (ADG) was 528.56 grams for the whole herd. The ADG was higher for the male (546.48 grams) than for the female (520.29 grams), and its was higher for the CT than the CC, but the difference was not statistically significant (P>0.05). The feed conversion ratio (FCR) was 2.69 kg. The estimated lean percentage at 8.5 months old was 64.08%. The results indicate that Piétrain stress negative pigs could develop well on the farm conditions in Hai Phong, Vietnam

Antibiotic Resistance Profile and Methicillin-Resistant Encoding Genes of Staphylococcus aureus Strains Isolated from Bloodstream Infection Patients in Northern Vietnam

Background:  Evaluating the antibiotic susceptibility and resistance genes is essential in the clinical management of bloodstream infections (BSIs). Nevertheless, there are still limited studies in Northern Vietnam. AIM: This study aimed to determine the antibiotic resistance profile and methicillin-resistant encoding genes of Staphylococcus aureus (S. aureus) causing BSIs in Northern Vietnam. METHODS: The cross-sectional study was done from December 2012 to June 2014 in two tertiary hospitals in Northern Vietnam. Tests performed at the lab of the hospital. RESULTS:  In 43 S. aureus strains isolating, 53.5 % were MRSA. Distribution of gene for overall, MRSA, and MSSA strains were following: mecA gene (58.1 %; 95.7%, and 15%), femA gene (48.8%, 47.8%, and 50%), femB gene (88.4%, 82.6%, and 95%). Antibiotic resistance was highest in penicillin (100%), followed by erythromycin (65.1%) and clindamycin (60.5%). Several antibiotics were susceptible (100%), including vancomycin, tigecycline, linezolid, quinupristin/dalfopristin. Quinolone group was highly sensitive, include ciprofloxacin (83.7%), levofloxacin (86%) and moxifloxacin (86%). CONCLUSION:  In S. aureus causing BSIs, antibiotic resistance was higher in penicillin, erythromycin, and clindamycin. All strains were utterly susceptible to vancomycin, tigecycline, linezolid, quinupristin/dalfopristin

Antibiotic Resistance Profile and Diversity of Subtypes Genes in Escherichia coli Causing Bloodstream Infection in Northern Vietnam

BACKGROUND: Evaluating the antibiotic susceptibility and resistance genes is essential in the clinical management of bloodstream infections (BSIs). But there are still limited studies in Northern Vietnam. AIM: The aim of the study was to determine the antibiotic resistance profile and characteristics of subtypes genes in Escherichia coli causing BSIs in Northern Vietnam. METHODS: The cross-sectional study was done in the period from December 2012 to June 2014 in two tertiary hospitals in Northern Vietnam. Tests were performed at the lab of the hospital. RESULTS: In 56 E. coli strains isolating 39.29 % produced ESBL. 100% of the isolates harbored blaTEM gene, but none of them had the blaPER gene. The prevalence of ESBL producers and ESBL non-producers in blaCTX-M gene was 81.82%, and 73.53%, in blaSHV gene was 18.18% and 35.29%. Sequencing results showed three blaTEM subtypes (blaTEM 1, 79, 82), four blaCTX-M subtypes (blaCTX-M-15, 73, 98, 161), and eight blaSHV subtypes (blaSHV 5, 7, 12, 15, 24, 33, 57, 77). Antibiotic resistance was higher in ampicillin (85.71%), trimethoprim/sulfamethoxazole (64.29%) and cephazolin (50%). Antibiotics were still highly susceptible including doripenem (96.43%), ertapenem (94.64%), amikacin (96.43%), and cefepime (89.29%). CONCLUSION: In Escherichia coli causing BSIs, antibiotic resistance was higher in ampicillin, trimethoprim/sulfamethoxazole and cephazolin. Antibiotics was highly susceptible including doripenem, ertapenem, amikacin, and cefepime

The Role of Serial NT-ProBNP Level in Prognosis and Follow-Up Treatment of Acute Heart Failure after Coronary Artery Bypass Graft Surgery

BACKGROUND: After coronary artery bypass graft (CABG) surgery, heart failure is still major problem. The valuable marker for it is needed. AIM: Evaluating the role of serial NT-proBNP level in prognosis and follow-up treatment of acute heart failure after CABG surgery. METHODS: The prospective, analytic study evaluated 107 patients undergoing CABG surgery at Ho Chi Minh Heart Institute from October 2012 to June 2014. Collecting data was done at pre- and post-operative days with measuring NT-proBNP levels on the day before operation, 2 hours after surgery, every next 24 h until the 5th day, and in case of acute heart failure occurred after surgery. RESULTS: On the first postoperative day (POD1), the NT-proBNP level demonstrated significant value for AHF with the cut-off point = 817.8 pg/mL and AUC = 0.806. On the second and third postoperative day, the AUC value of NT- was 0.753 and 0.751. It was statistically significant in acute heart failure group almost at POD 1 and POD 2 when analyzed by the doses of dobutamine, noradrenaline, and adrenaline (both low doses and normal doses). CONCLUSION: Serial measurement of NT-proBNP level provides useful prognostic and follow-up treatment information in acute heart failure after CABG surgery

Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco

Tobacco is an important commercial crop and a rich source of alkaloids for pharmaceutical and agricultural applications. However, its yield can be reduced by up to 70% due to virus infections, especially by a potyvirus Potato virus Y (PVY). The replication of PVY relies on host factors, and eukaryotic translation initiation factor 4Es (eIF4Es) have already been identified as recessive resistance genes against potyviruses in many plant species. To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing of two clades, eIF4E1 (eIF4E1-S and eIF4E1-T) and eIF4E2 (eIF4E2-S and eIF4E2-T) in the eIF4E gene family comprising six members in tobacco. We screened for CRISPR/Cas9-induced mutations by heteroduplex analysis and Sanger sequencing, and monitored PVY(O) accumulation in virus challenged regenerated plants by DAS-ELISA both in T0 and T1 generations. We found that all T0 lines carrying targeted mutations in the eIF4E1-S gene displayed enhanced resistance to PVY(O) confirming previous reports. More importantly, our combinatorial approach revealed that eIF4E1-S is necessary but not sufficient for complete PVY resistance. Only the quadruple mutants harboring loss-of-function mutations in eIF4E1-S, eIF4E1-T, eIF4E2-S and eIF4E2-T showed heritable high-level resistance to PVY(O) in tobacco. Our work highlights the importance of understanding host factor redundancy in virus replication and provides a roadmap to generate virus resistance by combinatorial CRISPR/Cas9-mediated editing in non-model crop plants with complex genomes

Safety and efficacy of fluoxetine on functional outcome after acute stroke (AFFINITY): a randomised, double-blind, placebo-controlled trial

Background Trials of fluoxetine for recovery after stroke report conflicting results. The Assessment oF FluoxetINe In sTroke recoverY (AFFINITY) trial aimed to show if daily oral fluoxetine for 6 months after stroke improves functional outcome in an ethnically diverse population. Methods AFFINITY was a randomised, parallel-group, double-blind, placebo-controlled trial done in 43 hospital stroke units in Australia (n=29), New Zealand (four), and Vietnam (ten). Eligible patients were adults (aged ≥18 years) with a clinical diagnosis of acute stroke in the previous 2–15 days, brain imaging consistent with ischaemic or haemorrhagic stroke, and a persisting neurological deficit that produced a modified Rankin Scale (mRS) score of 1 or more. Patients were randomly assigned 1:1 via a web-based system using a minimisation algorithm to once daily, oral fluoxetine 20 mg capsules or matching placebo for 6 months. Patients, carers, investigators, and outcome assessors were masked to the treatment allocation. The primary outcome was functional status, measured by the mRS, at 6 months. The primary analysis was an ordinal logistic regression of the mRS at 6 months, adjusted for minimisation variables. Primary and safety analyses were done according to the patient's treatment allocation. The trial is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12611000774921. Findings Between Jan 11, 2013, and June 30, 2019, 1280 patients were recruited in Australia (n=532), New Zealand (n=42), and Vietnam (n=706), of whom 642 were randomly assigned to fluoxetine and 638 were randomly assigned to placebo. Mean duration of trial treatment was 167 days (SD 48·1). At 6 months, mRS data were available in 624 (97%) patients in the fluoxetine group and 632 (99%) in the placebo group. The distribution of mRS categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio 0·94, 95% CI 0·76–1·15; p=0·53). Compared with patients in the placebo group, patients in the fluoxetine group had more falls (20 [3%] vs seven [1%]; p=0·018), bone fractures (19 [3%] vs six [1%]; p=0·014), and epileptic seizures (ten [2%] vs two [<1%]; p=0·038) at 6 months. Interpretation Oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and epileptic seizures. These results do not support the use of fluoxetine to improve functional outcome after stroke

Global, regional, and national burden of disorders affecting the nervous system, 1990–2021: a systematic analysis for the Global Burden of Disease Study 2021

BackgroundDisorders affecting the nervous system are diverse and include neurodevelopmental disorders, late-life neurodegeneration, and newly emergent conditions, such as cognitive impairment following COVID-19. Previous publications from the Global Burden of Disease, Injuries, and Risk Factor Study estimated the burden of 15 neurological conditions in 2015 and 2016, but these analyses did not include neurodevelopmental disorders, as defined by the International Classification of Diseases (ICD)-11, or a subset of cases of congenital, neonatal, and infectious conditions that cause neurological damage. Here, we estimate nervous system health loss caused by 37 unique conditions and their associated risk factors globally, regionally, and nationally from 1990 to 2021.MethodsWe estimated mortality, prevalence, years lived with disability (YLDs), years of life lost (YLLs), and disability-adjusted life-years (DALYs), with corresponding 95% uncertainty intervals (UIs), by age and sex in 204 countries and territories, from 1990 to 2021. We included morbidity and deaths due to neurological conditions, for which health loss is directly due to damage to the CNS or peripheral nervous system. We also isolated neurological health loss from conditions for which nervous system morbidity is a consequence, but not the primary feature, including a subset of congenital conditions (ie, chromosomal anomalies and congenital birth defects), neonatal conditions (ie, jaundice, preterm birth, and sepsis), infectious diseases (ie, COVID-19, cystic echinococcosis, malaria, syphilis, and Zika virus disease), and diabetic neuropathy. By conducting a sequela-level analysis of the health outcomes for these conditions, only cases where nervous system damage occurred were included, and YLDs were recalculated to isolate the non-fatal burden directly attributable to nervous system health loss. A comorbidity correction was used to calculate total prevalence of all conditions that affect the nervous system combined.FindingsGlobally, the 37 conditions affecting the nervous system were collectively ranked as the leading group cause of DALYs in 2021 (443 million, 95% UI 378–521), affecting 3·40 billion (3·20–3·62) individuals (43·1%, 40·5–45·9 of the global population); global DALY counts attributed to these conditions increased by 18·2% (8·7–26·7) between 1990 and 2021. Age-standardised rates of deaths per 100 000 people attributed to these conditions decreased from 1990 to 2021 by 33·6% (27·6–38·8), and age-standardised rates of DALYs attributed to these conditions decreased by 27·0% (21·5–32·4). Age-standardised prevalence was almost stable, with a change of 1·5% (0·7–2·4). The ten conditions with the highest age-standardised DALYs in 2021 were stroke, neonatal encephalopathy, migraine, Alzheimer's disease and other dementias, diabetic neuropathy, meningitis, epilepsy, neurological complications due to preterm birth, autism spectrum disorder, and nervous system cancer.InterpretationAs the leading cause of overall disease burden in the world, with increasing global DALY counts, effective prevention, treatment, and rehabilitation strategies for disorders affecting the nervous system are needed

Catalytic N-demethylation of alkaloids with an Feᴵᴵᴵ-TAML macrocycle and syntheses towards new TAML analogues

The N-demethylation of specific tropane and opiate alkaloids enables the synthesis of various medicines. This thesis investigates the use of an iron(III) tetraamido macrocycle (Feᴵᴵᴵ-TAML) as a catalyst for the oxidative N-demethylation of tropane and opiate alkaloids with hydrogen peroxide as a green oxidant. The syntheses of new tetraamide macrocycles are also reported. Initial investigations examined the oxidative N-demethylation of tropane alkaloids to their nortropane derivatives with H₂O₂. After screening various reaction conditions with atropine, the optimised conditions were applied to preparative N-demethylation of atropine and scopolamine to noratropine and norscopolamine, respectively, in high conversion and good yields in a simple one-pot process without any chromatography. Some by-products were tentatively identified and putative N-demethylation reaction pathways were proposed. Further investigations conclusively identified N-formyl-noratropine and carbon-hydroxylated tropane derivatives as by-products of the catalytic reaction with atropine. Additional studies indicated that Feᴵᴵᴵ-TAML catalyses the oxidative N-demethylation of atropine to noratropine via a biomimetic pathway involving the formation and then decomposition of a N-hydroxymethyl-noratropine intermediate. Factors such as substrate concentration, alcohol co-solvent and oxidant structure, rate of oxidant addition and concentration of water were found to influence the selectivity of N-demethylation vs. N-methyl oxidation to N-formyl-noratropine, whereas temperature mainly affected conversion efficiency. It was found that Feᴵᴵᴵ-TAML also catalysed the N-demethylation of the opiate alkaloids thebaine and oxycodone but with much less selectivity. A set of new tetraamide macrocycles were synthesized to enable future studies on the effect of Feᴵᴵᴵ-TAML structure on reaction selectivity with tropane and opiate alkaloids. These syntheses were achieved by preparing the requisite malonyl dichloride precursors which were then cyclized with a common diamide diamine precursor. Bond rotation and ring inversion were observed for two of these macrocycles on the N.M.R. timescale and their associated thermodynamic activation parameters were measured. These studies showed that the activation energy for bond rotation of geminal substituents is predominantly influenced by the macrocycle ring structure rather than substituent size, while the activation energy for ring inversion is significantly influenced by substituent size. In addition, ring inversion appears to proceed via a more-ordered transition state, suggesting an associative solvent-mediated process

Catalytic N-demethylation of alkaloids with an Feᴵᴵᴵ-TAML macrocycle and syntheses towards new TAML analogues

The N-demethylation of specific tropane and opiate alkaloids enables the synthesis of various medicines. This thesis investigates the use of an iron(III) tetraamido macrocycle (Feᴵᴵᴵ-TAML) as a catalyst for the oxidative N-demethylation of tropane and opiate alkaloids with hydrogen peroxide as a green oxidant. The syntheses of new tetraamide macrocycles are also reported. Initial investigations examined the oxidative N-demethylation of tropane alkaloids to their nortropane derivatives with H₂O₂. After screening various reaction conditions with atropine, the optimised conditions were applied to preparative N-demethylation of atropine and scopolamine to noratropine and norscopolamine, respectively, in high conversion and good yields in a simple one-pot process without any chromatography. Some by-products were tentatively identified and putative N-demethylation reaction pathways were proposed. Further investigations conclusively identified N-formyl-noratropine and carbon-hydroxylated tropane derivatives as by-products of the catalytic reaction with atropine. Additional studies indicated that Feᴵᴵᴵ-TAML catalyses the oxidative N-demethylation of atropine to noratropine via a biomimetic pathway involving the formation and then decomposition of a N-hydroxymethyl-noratropine intermediate. Factors such as substrate concentration, alcohol co-solvent and oxidant structure, rate of oxidant addition and concentration of water were found to influence the selectivity of N-demethylation vs. N-methyl oxidation to N-formyl-noratropine, whereas temperature mainly affected conversion efficiency. It was found that Feᴵᴵᴵ-TAML also catalysed the N-demethylation of the opiate alkaloids thebaine and oxycodone but with much less selectivity. A set of new tetraamide macrocycles were synthesized to enable future studies on the effect of Feᴵᴵᴵ-TAML structure on reaction selectivity with tropane and opiate alkaloids. These syntheses were achieved by preparing the requisite malonyl dichloride precursors which were then cyclized with a common diamide diamine precursor. Bond rotation and ring inversion were observed for two of these macrocycles on the N.M.R. timescale and their associated thermodynamic activation parameters were measured. These studies showed that the activation energy for bond rotation of geminal substituents is predominantly influenced by the macrocycle ring structure rather than substituent size, while the activation energy for ring inversion is significantly influenced by substituent size. In addition, ring inversion appears to proceed via a more-ordered transition state, suggesting an associative solvent-mediated process

Primary evaluation on growth performances of stress negative Piétrain pigs raised in Hai Phong province of Vietnam

peer reviewedThe present study was carried out on 19 stress negative Piétrain pigs (Pietrain ReHal), consisting of 13 gilts and 6 young boars imported from Belgium, raised in the livestock farm of Dong Hiep (Hai Phong) in order to evaluate growth performances and their adaptability in the North of Vietnam. Results showed that the average body weight of the whole herd at 2, 4, 5.5, and 8.5 months old was 19.05, 51.05, 85.82, and 119.47 kg, respectively. During the growing periods, except the first stage, the male grew faster than the female and the pigs of the CT genotype grew faster than those of CC genotype although the difference was not significant (P>0.05). The average daily gain (ADG) was 528.56 grams for the whole herd. The ADG was higher for the male (546.48 grams) than for the female (520.29 grams), and its was higher for the CT than the CC, but the difference was not statistically significant (P>0.05). The feed conversion ratio (FCR) was 2.69 kg. The estimated lean percentage at 8.5 months old was 64.08%. The results indicate that Piétrain stress negative pigs could develop well on the farm conditions in Hai Phong, Vietnam