Evaluation of Ensiled Brewer's Grain in the Diet of Piglets by One Way Multiple Analysis of Variance, MANOVA

The basic purpose of feeding trials is to find the optimum level of feed ingredients which give the highest economical returns to the farmers. This can be achieved through estimation and comparison of means of different rations. The example we have is a study of incorporation of different levels of ensiled brewers grains in the diet of 24 hybrids weaned piglets from Landrace x Duroc x Berkshire x Large White. They were randomly divided into four groups with three replicates of two piglets per pen. They were fed 0, 10, 20, 30% incorporation of ensiled brewer's grains on dry matter basis during post-weaning period followed by 0, 30, 40 and 50% during growing period and 0, 50, 60 and 70% during finishing period. We have one explanatory variable: initial weight, and four post treatment outcome variables recorded per piglets: final weight, dry matter consumption, weight gain and index of consumption. Comparing of several multivariate treatment means model design analysis is adapted. We obtain the MANOVA (Multiple Analyse of Variance) table of each phase, where the treatment differences exist by using Wilk's lambda distribution, and we find the treatment effect by using a confidence interval method of MANOVA. This model has the advantage of computing the responses of all variables in the matrix of sum of squares and more precisely in separation of the different means percentage of Ensiled Brewer's grain

Evaluation naturelle de l’impact de Beauveria bassiana : Champignon enthomopathogène dans la dynamique de population de Hypothenemus hampei, scolyte de baies des cerises de Coffea canefora

A study of finding out natural enemies of the coffee cherry borer beetle Coffea canephora, Hypothenemus hampei (Ferrari) (Coleoptera, Scolytidae) was carried out at two ecologically different sites of Cameroon: Nkoemvone in the South region of the country and Nkolbisson around Yaoundé in the central region. For six months, 500 berries were randomly collected monthly at each site. The presence of entomopathogenic fungus was observed on berries infested by the borer beetle. Rates of borer insect mortality due to Bauveria bassiana at Nkoemvone are: 70.6% in August, 64.6% in September, 32% in October, 43.2% in November, 39.2% in December and very low in January (8.6%). On the other hand, these rates at Nkolbisson are: 42.6% in October, 10% in November, 3.4% in December, 2.6% in January, 1.4% in February and 0% in March. From these observations, it was possible to carry out a research on the pathogenicity tests through its natural presence and on artificial-infection trials of the borer beetle with this fungus in order to protect better the coffee grains with a biological product

Pesticides use in cocoa sector in Cameroon: characterization of supply source, nature of actives ingredients, fashion and reasons for their utilization

The chemical control is the widely control method applied in Cameroon for pests and diseases by all the cocoa producers without technical suitable assistance by the State as before 1980’S. However, it is known that farmers have less knowledge on the good pesticides use for the control of a specific pest. The objective of this study was to provide insight in current pesticide use in the cocoa sector in Cameroon. A stratified sampling scheme was used. A total of 251 cocoa farmers, 20 post-harvest cocoa traders and 37 chemical retailers were randomly selected and interviewed. The study showed that 35 different chemicals were marketed in Cameroon for use in cocoa: 4 herbicides, 11 fungicides and 20 insecticides. Of 251 farmers consulted, 96.8% said that they use pesticides on their farms while 3.2% did not. Fungicides were used most often by farmers 61.8%) followed by insecticides (38.2%). Eight active ingredients although, officially banned, were still being used on cocoa farms. Over 77% of farmers do not respect the official spray recommendations for chemicals. Moreover, 64% do not respect recommended doses. Two main pesticide-supply-pathways exist in Cameroon: a legal and illegal supply chain, which provides 51% of pesticides to cocoa farmers, resulting in an estimated loss of value added tax for the Cameroonian government of about 550 000 to 2.4 million Euros per year. These results suggest that improved pests and diseases control by the State and raising farmer awareness about pesticide use in cocoa could greatly attribute to more sustainable cocoa economy in Cameroon.Keywords : Cocoa, pesticides, insecticides, fungicides, Cameroon

Diversity and pathogenicity of the Ceratocystidaceae associated with cacao agroforests in Cameroon

Knowledge of the diversity and ecology of plant pathogenic fungi in cacao agroforests and surrounding natural ecosystems can inform the development of sustainable management strategies for new cacao disease outbreaks. In this study, we investigated the occurrence of fungi related to the Ceratocystisdaceae and their nitidulid beetle vectors in cacao agroforests in Cameroon, under diverse agroecological conditions. The fungi and their vectors were collected from artificially induced stem wounds on cacao and associated shade trees. Collections were also made from abandoned cacao pod husks and other tree wounds within and around plantations. Fungal isolates were identified using DNA sequence-based phylogenies and morphological comparisons, and two representatives of each species were evaluated for pathogenicity on cacao. Five species of Ceratocystidaceae were recovered, including Huntiella chlamydoformis sp. nov., H. pycnanthi sp. nov. and H. moniliformis, as well as Thielaviopsis cerberus and Th. ethacetica. The incidence of these fungi appeared to be influenced by the prevailing agroecological conditions. Nitidulid beetles in the genus Brachypeplus were found to be their most common insect associates on cacao. Both Th. ethacetica and H. pycnanthi produced extensive lesions after inoculation on branches of mature cacao trees, while Th. ethacetica also caused pod rot. Although their impact remains unknown, fungi in the Ceratocystidaceae and their nitidulid beetle vectors are common and likely contribute to the parasitic pressure in Cameroonian cacao agrosystems.NRF-DST Centre of Excellence in Tree Health Biotechnology (CTHB) of South Africa and the Department of Corporate International Relations of the University of Pretoria through a postgraduate ‘study abroad grant’.http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-30592017-01-31hb2016Forestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant Patholog

Termite diversity across an anthropogenic disturbance gradient in the humid forest zone of West Africa

Data are presented for termite assemblages across an anthropogenic disturbance gradient in the humid forest zone of West and Central Africa. Sampling was by standardised 100m × 2m transects in: primary forest, several ages of regenerating forest, agroforestry plots, short fallows, mixed food crop fields, and mechanically cleared plots. Most sites were in southernCameroon—two additional transects were conducted in primary forest in Congo (Brazzaville). Species richness was negatively correlated with the disturbance gradient, although transects in areas with a complete or near-complete canopy were broadly similar in species richness. The strongest negative correlation was found for termite species feeding furthest down the humification gradient (group IV soil feeders) probably because these species are energetically constrained from living in non-forest habitats. In contrast species feeding and nesting in dead wood (group I wood feeders) termites, which live in the most protected micro-habitats, were significantly positively correlated with the disturbance gradient. Species composition was also strongly correlated with disturbance level in multivariate redundancy analyses. Pest species were an insignificant element of the assemblage, in part because of the distance of the sites from savanna source pools. Overall, it appears that termite-mediated wood and litter decomposition may be relatively unaffected by light to moderate disturbance intensity, but that soil-conditioning by termites might be greatly reduced in agricultural land cleared from tropical forest

Termite diversity across an anthropogenic disturbance gradient in the humid forest zone of West Africa

Data are presented for termite assemblages across an anthropogenic disturbance gradient in the humid forest zone of West and Central Africa. Sampling was by standardised 100 mx2 m transects in: primary forest, several ages of regenerating forest, agroforestry plots, short fallows, mixed food crop fields, and mechanically cleared plots. Most sites were in southern Cameroon––two additional transects were conducted in primary forest in Congo (Brazzaville). Species richness was negatively correlated with the disturbance gradient, although transects in areas with a complete or near-complete canopy were broadly similar in species richness. The strongest negative correlation was found for termite species feeding furthest down the humification gradient (group IV soil feeders) probably because these species are energetically constrained from living in non-forest habitats. In contrast species feeding and nesting in dead wood (group I wood feeders) termites, which live in the most protected micro-habitats, were significantly positively correlated with the disturbance gradient. Species composition was also strongly correlated with disturbance level in multivariate redundancy analyses. Pest species were an insignificant element of the assemblage, in part because of the distance of the sites from savanna source pools. Overall, it appears that termite-mediated wood and litter decomposition may be relatively unaffected by light to moderate disturbance intensity, but that soil-conditioning by termites might be greatly reduced in agricultural land cleared from tropical forest

EVALUATION NATURELLE DE L\u2019IMPACT DE BEAUVERIA BASSIANA : CHAMPIGNON ENTHOMOPATHOG\uc9NE DANS LA DYNAMIQUE DE POPULATION DE HYPOTHENEMUS HAMPEI , SCOLYTE DE BAIES DES CERISES DE COFFEA CANEFORA COFFEA CANEFORA

A study of finding out natural enemies of the coffee cherry borer beetle Coffea canephora , Hypothenemus hampei (Ferrari) (Coleoptera, Scolytidae) was carried out at two ecologically different sites of Cameroon: Nkoemvone in the South region of the country and Nkolbisson around Yaound\ue9 in the central region. For six months, 500 berries were randomly collected monthly at each site. The presence of entomopathogenic fungus was observed on berries infested by the borer beetle. Rates of borer insect mortality due to Bauveria bassiana at Nkoemvone are: 70.6% in August, 64.6% in September, 32% in October, 43.2% in November, 39.2% in December and very low in January (8.6%). On the other hand, these rates at Nkolbisson are: 42.6% in October, 10% in November, 3.4% in December, 2.6% in January, 1.4% in February and 0% in March. From these observations, it was possible to carry out a research on the pathogenicity tests through its natural presence and on artificial-infection trials of the borer beetle with this fungus in order to protect better the coffee grains with a biological product.Une \ue9tude de recherche des ennemis naturels contre le scolyte des cerises de caf\ue9ier Coffea canephora , Hypothenemus hampei (Ferrari) (Coleoptera, Scolytidae), a \ue9t\ue9 men\ue9e dans deux sites au Cameroun : Nkoemvone une r\ue9gion du Sud du pays et Nkolbisson pr\ue8s de Yaound\ue9, une r\ue9gion du centre. Pendant six mois, 500 baies \ue9taient mensuellement pr\ue9lev\ue9es de mani\ue8re al\ue9atoire dans chaque site. La pr\ue9sence du champignon entomopathog\ue8nes a \ue9t\ue9 observ\ue9e sur les baies attaqu\ue9es par les scolytes. Les taux de mortalit\ue9 du scolyte due \ue0 Bauveria bassiana \ue0 Nkoemvone sont de 70,6 en ao\ufbt, 64,6% en septembre, 32% en octobre, 43,2% en Novembre, 39,2% en d\ue9cembre et tr\ue8s faible en janvier (8,6%). Par contre ce taux \ue0 Nkolbisson est de 42,6% en octobre, 10% en Novembre, 3,4% en d\ue9cembre, 2,6%en janvier, 1,4% en f\ue9vrier et 0% en Mars. A partir de ces observations, il a \ue9t\ue9 possible de proposer par sa pr\ue9sence naturel une cherche sur le test de pathogenicit\ue9 et des essais d\u2019infection artificielle du scolyte avec ce champignon \ue0 fin mieux prot\ue9ger les grains de caf\ue9ier avec un produit biologique