Clinical and histopathological correlation of breast lesions

Background: To study the histopathological features of neoplastic and non neoplastic lesions of breast.  To correlate the pathological findings with clinical parameters.Methods: We have studied total 170 cases of breast lesions over a period of two years in our institute. The specimens were received in histopathology section of our department. Detailed gross examination of specimens was done followed by fixation, thorough sampling, and tissue processing. The different lesions were studied by histopathological examination and analysed. Neoplastic lesions were classified according to the WHO classification.Results: Out of the 170 cases, 128 cases had neoplastic lesions and 41 cases had non-neoplastic lesions, and one case had coexistent neoplastic and nonneoplastic lesions. Out of the total 129 cases with neoplastic lesions, 76 cases had benign breast tumors, 51 cases had malignant breast tumors, and 2 cases had precursor lesions. Fibroadenoma was the most common benign tumour with 62 cases. Invasive carcinoma no special type was the most common malignant tumour with 43 cases. Special subtypes of invasive carcinoma found in our study were mucinous carcinoma (2 case). The most common nonneoplastic lesion was mastitis with 12 cases, followed by duct ectasia and fibrocystic change. There were 6 cases of gynaecomastia. All the tumors involved upper outer quadrant most frequently. The benign tumors were most frequent in second, third and fourth decades, malignant tumours were seen beyond 4th decade. The nonneoplastic lesions were common in 4th decade.Conclusions: Histopathological study is important in the management of breast lesions

Mesoscale structure and techno-functional properties of enzymatically cross-linked a-lactalbumin nanoparticles

Abstract The aim of this thesis is to understand the connection between molecular, meso and macroscales of enzymatically cross-linked proteins. It was hypothesised that the techno-functional properties at macroscale, such as bulk rheology and foam stability, are affected by the structure of nanoparticles at mesoscale. The approach was to make α-lactalbumin (α-LA) nanoparticles by using two different enzymes, horseradish peroxidase (HRP) or microbial transglutaminase (mTG), to produce an open and compact mesoscale structure, respectively. In addition to the control over the mesoscale structure, the size of the nanoparticles can be independently controlled by varying the dosage of hydrogen peroxide in the case of HRP and by thermal inactivation in the case of mTG. The other important parameters determining the size are protein concentration and ionic strength. The size (radius of gyration) range that could be achieved by varying the above mentioned control parameters is 20 – 200 nm. The polydispersed nanoparticles were separated by asymmetrical flow field flow fractionation (AF4) and characterised inline with multi angle light scattering (MALS). Polymerization of apo α-LA with HRP and mTG proceeds in a step growth way i.e. first monomers react to form oligomers and the oligomers are cross-linked to form polymers (nanoparticles). Extensive cross-linking of α-LA with HRP gives rise to not only di-tyrosine cross-links, but also tri–octa tyrosine cross-links, which was hitherto unknown. The two different mesoscale structures result in gels of different storage moduli. The storage modulus of gels made by concentrating the α-LA/mTG nanoparticles was around ten times higher than that made with open nanoparticles. The half-life time (t0.5) of the foam made with α-LA nanoparticles was two to six times higher than that of the monomeric α-LA. The higher foam-stability of the α-LA nanoparticles as compared to the monomeric α-LA is due to their higher thickness of the interfacial layer and thin films. In conclusion, it is shown that the techno-functional properties of α-LA are directly correlated to the size and meso-scale structures of the nanoparticles and enzymatic cross-linking is an effective way to control them. </p

Approximate Controllability of Delayed Fractional Stochastic Differential Systems with Mixed Noise and Impulsive Effects

We herein report a new class of impulsive fractional stochastic differential systems driven by mixed fractional Brownian motions with infinite delay and Hurst parameter $\hat{\cal H} \in ( 1/2, 1)$. Using fixed point techniques, a $q$-resolvent family, and fractional calculus, we discuss the existence of a piecewise continuous mild solution for the proposed system. Moreover, under appropriate conditions, we investigate the approximate controllability of the considered system. Finally, the main results are demonstrated with an illustrative example.Comment: Please cite this paper as follows: Hakkar, N.; Dhayal, R.; Debbouche, A.; Torres, D.F.M. Approximate Controllability of Delayed Fractional Stochastic Differential Systems with Mixed Noise and Impulsive Effects. Fractal Fract. 2023, 7, 104. https://doi.org/10.3390/fractalfract702010

Differential effects of interleukin-13 and interleukin-6 on Jak/STAT signaling and cell viability in pancreatic β-cells

This is an Accepted Manuscript of an article published by Taylor & Francis in islets on 1 March 2013, available online: http://www.tandfonline.com/10.4161/isl.24249Open Access articlePro-inflammatory cytokines are important mediators of β-cell demise in type 1 diabetes, and similar mechanisms are increasingly implicated in type 2 diabetes, where a state of chronic inflammation may persist. It is likely that the actions of anti-inflammatory cytokines are also altered in diabetes. Cytokines are released from immune cells, which may be recruited to the islets in diabetes, but they can also be produced by islet endocrine cells in response to environmental factors, including enteroviral infection. Since enteroviral infection of islet cells may influence the development of diabetes in humans, we examined the actions of two cytokines, IL-13 and IL-6, whose expression are reported to be altered in β-cells during enteroviral infection. Human and rodent islet cells were shown to express receptors for both IL-13 and IL-6, and treatment with either cytokine resulted in the rapid phosphorylation of STAT3 and STAT6. However, while β-cells were protected against a range of cytotoxic insults during exposure to IL-13, treatment with IL-6 enhanced cytotoxicity and western blotting revealed that IL-13 induced one specific isoform of phospho-STAT6 preferentially. Upon incubation with both cytokines together, the isoform of STAT6 that was upregulated by IL-13 alone was again induced, and the effects of IL-6 on β-cell viability were attenuated. Overall, the results suggest that induction of specific isoforms of STAT family transcription factors may underlie the cytoprotective actions of IL-13, and they imply that selective targeting of specific STAT-mediated signaling components could provide a means to ameliorate the loss of β-cell viability in diabetes.Nuffield Foundation - Bursary schemeEuropean Union’s Seventh Framework Programme PEVNET (FP7/2007-2013

Effect of foliar application of zinc and salicylic acid on growth, flowering and chemical constitute of African marigold cv. pusa narangi gainda (Targets erecta L.)

A field experiment on African marigold (Targets erecta L.) was conducted during winter season of 2014-15to study the foliar effect of Zn and SA of 20 treatment combinations having five concentrations of zinc (0.0, 0.25, 0.50, 0.75, and 1.0 %) and salicylic acid (0.0, 0.25, 0.50 and 1.0 mM/L).The treatmentZn4SA3 (Zinc 1% + Salicylic acid 1.0 mM/L) recorded the maximum plant height (77.41 cm), number of leaves per plant (314.10),earliest first flower bud appearance (39.78 days), maximum number of flowers per plant (62.33), maximum chlorophyll content (3.83mg/g) and maximum carotene content (3.07 mg/g)as compared to control where it was recorded minimum. These results are conclusive that foliar spraying with zinc 1.0% + salicylic acid 1.0 mM/L may positively increasedthe growth and flowering parametersof marigold

Chitosan/polyaniline hybrid conducting biopolymer base impedimetric immunosensor to detect Ochratoxin-A

Chitosan (CS)–polyaniline (PANI) hybrid conducting biopolymer film was obtained on indium–tin-oxide (ITO) electrode using electrochemical polymerization process. Fourier transform infrared (FT-IR) spectra of PANI–CS had showed covalent and hydrogen binding between PANI and CS molecules. Electrochemical impedance spectroscopy (EIS) measurements had showed lowcharge transfer resistance (RCT) of PANI–CS and PANI. Successive rabbit antibody (IgGs) immobilizationonPANI–CS, CS and PANI matrixes surfacewere confirmed with FT-IR and EIS measurements. Ochratoxin-A (OTA) interaction with IgGs had increased RCT values and showed linear response up to 10 ng/mL OTA concentration in electrolyte. Relative change in RCT was higher in PANI–CS due to higher proportion of carboxylic and hydroxyl functionalities at PANI–CS matrix surfaces. The absolute sensitivity of PANI, CS, and PANI–CSwere 16±6, 22±9 and 53±8�mL/ng, respectively derived fromslope of linear response up to 10 ng/mL with 1 ng/mL minimum detection limit

Response of okra (Abelmoschus esculentus L. Moench) to vermicompost, mycorrhiza and micronutrients mixture

A field experiments on okra (Abelmoschus esculentus&nbsp;L Moench) cv. Arka Anamika” was conducted at Department of Vegetable Science, College of Horticulture and Forestry, Jhalrapatan city, Jhalawar during the Kharif 2016-17 to study the effect of vermicompost, mycorrhiza and micronutrients mixture of 16 treatment combination i.e. vermicompost with two levels (2 tons/ha and 4 tons /ha), mycorrhiza with one level (Mycorrhiza seed treatment) and micronutrients mixture with two levels (50 ppm and 100 ppm). The observations revealed that the plant height of&nbsp;A. esculentus&nbsp;after 60 DAS (74 cm), plant height after 90 DAS (79.71cm), number of leaves / plant (43.17), main steam diameter (5.52 cm), number of primary branches per plant (5.10) of&nbsp;A. esculentus&nbsp;and lowest days to first flower bud emergence (33.53) of A. esculentus was noted maximum as compared to control. The results indicates that plant height, number of leaves per plant, main stem diameter, stem girth, number of primary branches increased significantly due to application of different levels of vermicompost, mycorrhiza and micronutrients mixture as compared to control. It is postulated that the vermicompost 2tons and 4tons + mycorrhiza seed treatment + micronutrients mixture 50ppm and 100ppm may positively regulate the&nbsp;A. esculentus&nbsp;growth improved. Therefore, application of vermicompost, mycorrhiza along with a mixture of micronutrients played a significant role on enhancing the growth of okra (A. esculentus) and can be applied to obtain the maximum crop yield of okra (A. esculentus)

Qualitative and Quantitative Evaluation of Flavonoids from Corchorus olitorius L.

Corchorus olitorius is fibre yielding plant with nutritive value so human used its various parts in their food. Flavonoids&nbsp; are secondary metabolites&nbsp; of plants and exist with variable phenolic structures.In the present study carried out the qualitative and quantitative analysis of flavonoids by using chromatographic and spectral studies.Total amount of flavonoids were&nbsp; found in Corchorus olitorius ( 0.55 mg/gdw in stem and 0.30 mg/gdw in fruit). Thirty three compound were found in GC-MS analysis, Ethyl Oleate found to be maximum from fruits of Corchorus olitorius. Keywords: GC-MS,flavonoids , Corchorus olitorius

IbM KELURAHAN CIPTOMULYO KOTA MALANG DALAM MENGOLAH DAUN TANAMAN ALLOE VERA SEBAGAI BAHAN UTAMA KOMODITAS PRODUK MAKANAN

Kegiatan IbM kelurahan Ciptomulyo didanai oleh DIKTI, melalui penugasan untuk pelaksanaan Program Hibah Pengabdian pada Masyarakat mono tahun bagi dosen ini, secara garis besar dikategorikan kedalam 2(dua) aktifitas utama yang saling berketerkaitan. yaitu :aspek Produksi dan aspek Manajemen Usaha. Produksi makanan jeli dengan bahan baku utama adalah daun aloevera diharapkan akan menjadi penciri produk dari kelurahan Ciptomulyo Kota Malang. Hal tersebut dikarenakan belum banyak pemanfaatan daun aloevera sebagai makanan jeli. Saat ini aloevera digunakan sebagai bahan dasar pembuatan produk kosmetik. Gambaran dari Teknologi terapan yang digunakan meliputi mesin mixer yang digunakan untuk memproses daun aloe vera ini dilengkapi dengan pemanas berupa kompor gas dan juga dipasangkan pengendali suhu atau temperature. Dengan kata lain bahwa mesin ini merupakan integrasi dari fungsi pengaduk, pemanas dan pengendali suhu. Mesin ini tepat digunakan untuk tujuan proses pengentalan dari daun aloevera dengan karakter dasarnya adalah lembek menjadi produk makanan Jeli dengan karakter dasarnya adalah kenyal. Untuk mencapai tingkat kekenyalan produk jeli dibutuhkan suhu 70 0 C