Progesterone regulation of implantation-related genes: new insights into the role of oestrogen

Genomic profiling was performed on explants of late proliferative phase human endometrium after 24-h treatment with progesterone (P) or oestradiol and progesterone (17β-E2+P) and on explants of menstrual phase endometrium treated with 17β-E2+P. Gene expression was validated with real-time PCR in the samples used for the arrays, in endometrium collected from early and mid-secretory phase endometrium, and in additional experiments performed on new samples collected in the menstrual and late proliferative phase. The results show that late proliferative phase human endometrium is more responsive to progestins than menstrual phase endometrium, that the expression of several genes associated with embryo implantation (i.e. thrombomodulin, monoamine oxidase A, SPARC-like 1) can be induced by P in vitro, and that genes that are fully dependent on the continuous presence of 17β-E2 during P exposure can be distinguished from those that are P-dependent to a lesser extent. Therefore, 17β-E2 selectively primes implantation-related genes for the effects of P

Complement Regulates Nutrient Influx and Metabolic Reprogramming during Th1 Cell Responses.

Expansion and acquisition of Th1 cell effector function requires metabolic reprogramming; however, the signals instructing these adaptations remain poorly defined. Here we found that in activated human T cells, autocrine stimulation of the complement receptor CD46, and specifically its intracellular domain CYT-1, was required for induction of the amino acid (AA) transporter LAT1 and enhanced expression of the glucose transporter GLUT1. Furthermore, CD46 activation simultaneously drove expression of LAMTOR5, which mediated assembly of the AA-sensing Ragulator-Rag-mTORC1 complex and increased glycolysis and oxidative phosphorylation (OXPHOS), required for cytokine production. T cells from CD46-deficient patients, characterized by defective Th1 cell induction, failed to upregulate the molecular components of this metabolic program as well as glycolysis and OXPHOS, but IFN-γ production could be reinstated by retrovirus-mediated CD46-CYT-1 expression. These data establish a critical link between the complement system and immunometabolic adaptations driving human CD4(+) T cell effector function

Sivas il merkezi özel ve devlet okullarındaki 12-17 yaş grubu öğrencilerin periodontal tedavi gereksiniminin ve dolgu ihtiyaçlarının karşılaştırılması

Objective: The purpose of this study was to determine the periodontal treatment needs, using the CPITN and tooth decay, in 12-17 years old children from private and public schools from Sivas. Material and method: A total of 838 children was studied (235 in private schools and 603 in public schools). Results: There was statistical difference between children from private and public schools regarding plaque index, gingival index and CPITN. There was no statistical difference regarding the number of missing and decayed teeth. The number of the filled teeth in private schools were more than public schools. Conclusion: There occured an idea concerning to give more information and an intensive treatment approach for the students from the public schools of the city. ÖZET Amaç: Bu çalışmanın amacı; CPITN kullanılarak, Sivas İl Merkezi özel ve devlet okullarındaki 12-17 yaş grubu öğrencilerin periodontal tedavi gereksinimi ve çürük diş dağılımının belirlenmesidir. Gereç ve yöntem: Toplam 838 öğrencide (235 özel okul ve 603 devlet okulu öğrencisi) çalışma yapılmıştır. Bulgular: Gingival indeks, plak indeksi, CPITN bakımından özel okul öğrencileri ile devlet okulu öğrencileri arasındaki fark anlamlı bulunmuştur. Çürük ve eksik diş sayısı arasında fark bulunmazken, özel okul öğrencilerinde dolgulu diş sayısı daha fazla tespit edilmiştir. Sonuç: Devlet okullarında okuyan öğrencilerin daha fazla bilgilendirilmeleri ve daha yoğun bir tedavi yaklaşımının olması yönünde bir fikir birliği oluşmuştur. Anahtar kelimeler: Gingival indeks, plak indeksi, CPIT

The Adjunctive Effect of Tenoxicam During Non-Surgical Periodontal Treatment on Clinical Parameters and Gingival Crevicular Fluid Levels of Mmp-8 and Tnf-Α in Patients with Chronic Periodontitis - Randomized, Double-Blind Clinical Trial

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