1,490 research outputs found
The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli
Skeletal metastases are frequently accompanied by chronic pain that is mechanoceptive in nature. Mechanistically, cancer-induced bone pain (CIBP) is mediated by peripheral sensory neurons innervating the cancerous site, the cell bodies of which are housed in the dorsal root ganglia (DRG). How these somatosensory neurons encode sensory information in CIBP remains only partly explained. Using a validated rat model, we first confirmed cortical bone destruction in CIBP but not sham-operated rats (day 14 after surgery, designated âlateâ-stage bone cancer). This occurred with behavioural mechanical hypersensitivity (KruskalâWallis H for independent samples; CIBP vs sham-operated, day 14; P < 0.0001). Next, hypothesising that the proportion and phenotype of primary afferents would be altered in the disease state, dorsal root ganglia in vivo imaging of genetically encoded calcium indicators and Markov Cluster Analysis were used to analyse 1748 late-stage CIBP (n = 10) and 757 sham-operated (n = 9), neurons. Distinct clusters of responses to peripheral stimuli were revealed. In CIBP rats, upon knee compression of the leg ipsilateral to the tumour, (1) 3 times as many sensory afferents responded (repeated-measures analysis of variance: P < 0.0001 [vs sham]); (2) there were significantly more small neurons responding (KruskalâWallis for independent samples (vs sham): P < 0.0001); and (3) approximately 13% of traced tibial cavity afferents responded (no difference observed between CIBP and sham-operated animals). We conclude that an increased sensory afferent response is present in CIBP rats, and this is likely to reflect afferent recruitment from outside of the bone rather than increased intraosseous afferent activity
Toxoplasmosis in Zoo Animals
Toxoplasma gondii is an extremely successful zoonotic protozoan parasite that has been demonstrated in a wide range of endo- and poikilothermic species. Although infection is widespread amongst domestic animals, overt disease other than abortion in small ruminants is sporadic. This survey evaluates toxoplasmosis in zoo animals based on a systematic review of pathology archive material (n = 33,506 submissions) over a 16-year study period. A total of 126 submissions, deriving from 32 zoos, two educational facilities and two private owners, were included in the study, based on gross lesions, cytological, histological and immunohistological diagnosis of toxoplasmosis. Clinical history, signalment, annual distribution and post-mortem findings were evaluated. A total of 31 species (mammalian 97%/avian 3%) were represented in the study material. Ring-tailed lemurs, slender tailed meerkats, Pallasâ cats, and squirrel monkeys were most affected. An unusual outbreak occurred in Asian small-clawed otters, in which toxoplasmosis has not been reported to date. Clinically, animals over 12 months of age presented with non-specific symptoms (anorexia, weight loss, lethargy, debilitation), neurological, gastrointestinal or respiratory signs and sudden death. Systemic disease predominated, with a propensity for encephalitis in meerkats and Pallasâ cats and systemic disease involving lymphoid tissues in ring-tailed lemurs. Cases in the UK occurred year-round, with species-specific peaks and increases between August and November. This study reinforces the importance of toxoplasmosis as a significant cause of sporadic and epizootic mortalities in a wide range of zoo animals. Feral cat control is crucial to reduce infection pressure
Lebesgue regularity for differential difference equations with fractional damping
We provide necessary and sufficient conditions for the existence and unique-ness of solutions belonging to the vector-valued space of sequences ïżœ(Z, X) forequations that can be modeled in the formÎu(n)+Îu(n)=Au(n)+G(u)(n)+ (n), n â Z,,>0,â„0,where X is a Banach space, â ïżœ(Z, X), A is a closed linear operatorwith domain D(A) defined on X,andG is a nonlinear function. The oper-ator Îdenotes the fractional difference operator of order >0inthesense of GrĂŒnwald-Letnikov. Our class of models includes the discrete timeKlein-Gordon, telegraph, and Basset equations, among other differential differ-ence equations of interest. We prove a simple criterion that shows the existenceof solutions assuming that f is small and that G is a nonlinear term
Effects of strong density fluctuations at the plasma edge on ECEI measurements at ASDEX Upgrade
EUROfusion Consortium 63305
Effects of density gradients and fluctuations at the plasma edge on ECEI measurements at ASDEX Upgrade
Electron cyclotron emission imaging (ECEI) provides measurements of electron temperature (Te) and its fluctuations (ÎŽTe). However, when measuring at the plasma edge, in the steep gradient region, radiation transport effects must be taken into account. It is shown that due to these effects, the scrape-off layer region is not accessible to the ECEI measurements in steady state conditions and that the signal is dominated by the shine-through emission. Transient effects, such as filaments, can change the radiation transport locally, but cannot be distinguished from the shine-through. Local density measurements are essential for the correct interpretation of the electron cyclotron emission, since the density fluctuations influence the temperature measurements at the plasma edge. As an example, a low frequency 8 kHz mode, which causes 10%â15% fluctuations in the signal level of the ECEI, is analysed. The same mode has been measured with the lithium beam emission spectroscopy density diagnostic, and is very well correlated in time with high frequency magnetic fluctuations. With radiation transport modelling of the electron cyclotron radiation in the ECEI geometry, it is shown that the density contributes significantly to the radiation temperature (Trad) and the experimental observations have shown the amplitude modulation in both density and temperature measurements. The poloidal velocity of the low frequency mode measured by the ECEI is 3 km sâ1. The calculated velocity of the high frequency mode measured with the magnetic pick-up coils is about 25 km sâ1. Velocities are compared with the E Ă B background flow velocity and possible explanations for the origin of the low frequency mode are discussed.EUROfusion Consortium 63305
Forces between clustered stereocilia minimize friction in the ear on a subnanometre scale
The detection of sound begins when energy derived from acoustic stimuli
deflects the hair bundles atop hair cells. As hair bundles move, the viscous
friction between stereocilia and the surrounding liquid poses a fundamental
challenge to the ear's high sensitivity and sharp frequency selectivity. Part
of the solution to this problem lies in the active process that uses energy for
frequency-selective sound amplification. Here we demonstrate that a
complementary part involves the fluid-structure interaction between the liquid
within the hair bundle and the stereocilia. Using force measurement on a
dynamically scaled model, finite-element analysis, analytical estimation of
hydrodynamic forces, stochastic simulation and high-resolution interferometric
measurement of hair bundles, we characterize the origin and magnitude of the
forces between individual stereocilia during small hair-bundle deflections. We
find that the close apposition of stereocilia effectively immobilizes the
liquid between them, which reduces the drag and suppresses the relative
squeezing but not the sliding mode of stereociliary motion. The obliquely
oriented tip links couple the mechanotransduction channels to this least
dissipative coherent mode, whereas the elastic horizontal top connectors
stabilize the structure, further reducing the drag. As measured from the
distortion products associated with channel gating at physiological stimulation
amplitudes of tens of nanometres, the balance of forces in a hair bundle
permits a relative mode of motion between adjacent stereocilia that encompasses
only a fraction of a nanometre. A combination of high-resolution experiments
and detailed numerical modelling of fluid-structure interactions reveals the
physical principles behind the basic structural features of hair bundles and
shows quantitatively how these organelles are adapted to the needs of sensitive
mechanotransduction.Comment: 21 pages, including 3 figures. For supplementary information, please
see the online version of the article at http://www.nature.com/natur
Toward the use of temporary tattoo electrodes for impedancemetric respiration monitoring and other electrophysiological recordings on skin
The development of dry, ultra-conformable and unperceivable temporary tattoo electrodes (TTEs), based on the ink-jet printing of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) on top of commercially available temporary tattoo paper, has gained increasing attention as a new and promising technology for electrophysiological recordings on skin. In this work, we present a TTEs epidermal sensor for real time monitoring of respiration through transthoracic impedance measurements, exploiting a new design, based on the application of soft screen printed Ag ink and magnetic interlink, that guarantees a repositionable, long-term stable and robust interconnection of TTEs with external âdockingâ devices. The efficiency of the TTE and the proposed interconnection strategy under stretching (up to 10%) and over time (up to 96 h) has been verified on a dedicated experimental setup and on humans, fulfilling the proposed specific application of transthoracic impedance measurements. The proposed approach makes this technology suitable for large-scale production and suitable not only for the specific use case presented, but also for real time monitoring of different bio-electric signals, as demonstrated through specific proof of concept demonstrators
Optimum Small Optical Beam Displacement Measurement
We derive the quantum noise limit for the optical beam displacement of a
TEM00 mode. Using a multimodal analysis, we show that the conventional split
detection scheme for measuring beam displacement is non-optimal with 80%
efficiency. We propose a new displacement measurement scheme that is optimal
for small beam displacement. This scheme utilises a homodyne detection setup
that has a TEM10 mode local oscillator. We show that although the quantum noise
limit to displacement measurement can be surpassed using squeezed light in
appropriate spatial modes for both schemes, the TEM10 homodyning scheme
out-performs split detection for all values of squeezing.Comment: 13 pages, 7 figure
The quest for companions to post-common envelope binaries. II. NSVS14256825 and HS0705+6700
We report new mid-eclipse times of the two close binaries NSVS14256825 and
HS0705+6700, harboring an sdB primary and a low-mass main-sequence secondary.
Both objects display clear variations in the measured orbital period, which can
be explained by the action of a third object orbiting the binary. If this
interpretation is correct, the third object in NSVS14256825 is a giant planet
with a mass of roughly 12 M_Jup. For HS0705+6700, we provide evidence that
strengthens the case for the suggested periodic nature of the eclipse time
variation and reduces the uncertainties in the parameters of the brown dwarf
implied by that model. The derived period is 8.4 yr and the mass is 31 M_Jup,
if the orbit is coplanar with the binary. This research is part of the
PlanetFinders project, an ongoing collaboration between professional
astronomers and student groups at high schools.Comment: Accepted by Astron. and Astrophy
Gain in Stochastic Resonance: Precise Numerics versus Linear Response Theory beyond the Two-Mode Approximation
In the context of the phenomenon of Stochastic Resonance (SR) we study the
correlation function, the signal-to-noise ratio (SNR) and the ratio of output
over input SNR, i.e. the gain, which is associated to the nonlinear response of
a bistable system driven by time-periodic forces and white Gaussian noise.
These quantifiers for SR are evaluated using the techniques of Linear Response
Theory (LRT) beyond the usually employed two-mode approximation scheme. We
analytically demonstrate within such an extended LRT description that the gain
can indeed not exceed unity. We implement an efficient algorithm, based on work
by Greenside and Helfand (detailed in the Appendix), to integrate the driven
Langevin equation over a wide range of parameter values. The predictions of LRT
are carefully tested against the results obtained from numerical solutions of
the corresponding Langevin equation over a wide range of parameter values. We
further present an accurate procedure to evaluate the distinct contributions of
the coherent and incoherent parts of the correlation function to the SNR and
the gain. As a main result we show for subthreshold driving that both, the
correlation function and the SNR can deviate substantially from the predictions
of LRT and yet, the gain can be either larger or smaller than unity. In
particular, we find that the gain can exceed unity in the strongly nonlinear
regime which is characterized by weak noise and very slow multifrequency
subthreshold input signals with a small duty cycle. This latter result is in
agreement with recent analogue simulation results by Gingl et al. in Refs. [18,
19].Comment: 22 pages, 5 eps figures, submitted to PR
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