Evaluation of the use of serological and bacteriological investigation for monitoring and controlling Salmonella in Italian pig herds

At the European level the control of foodborne diseases is defined by the new zoonoses legislation (Directive 20031991EC and Regulation (EC) No 2160/2003), which points out the necessity to establish surveillance programmes for zoonotic agents in animal populations. Recently Commission Decision 2006/6681EC concerning a baseline study on the prevalence of Salmonella in slaughter pigs has been published. Many different strategies have been developed and applied by EU Member States in order to Implement momtoring and/or control programmes for Salmonella in pigs; these strategies are mamly based on bacteriological analysis (performed on caecal content, ileo caecal lymph nodes or carcass swabs collected at slaughterhouse) and/or on serological analysis (mainly performed on meat juice obtained from diaphragm muscle)

Description of a Sarcoptic Mange Outbreak in Alpine Chamois Using an Enhanced Surveillance Approach

Since 1995, the Alpine chamois (Rupicapra r. rupicapra) population of the Dolomites has been affected by sarcoptic mange with considerable management concerns. In this study, 15 years (2006–2020) of passive surveillance and demographic data were analyzed in order to describe a mange outbreak. Furthermore, an enhanced passive surveillance protocol was implemented in order to evaluate the efficiency of ordinary vs. enhanced surveillance protocol in identifying dead chamois in the field and in reaching a correct diagnosis. Our results confirm the role of mange as a determining factor for chamois mortality, while stressing the importance of a wider view on the factors affecting population dynamics. The enhanced passive surveillance protocol increased the probability of carcass retrieval and identification of the cause of death; however, its adoption may be too costly if applied for long periods on a wide scale. Passive surveillance, in both ordinary and enhanced surveillance protocol, should encompass the use of other strategies in the future to study the eco-epidemiology of the disease in wild Caprinae

Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica

[EN] Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods: Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results: ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions: Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populationsSIThe Consejería de Medio Ambiente y Ordenación del Territorio of the Junta de Andalucía supported sample collection and conservation through the projects 676/2006/A/00, 1571/2007/M/00, 173/2009/M/00 and 861/2011/M/ 00, as did the PAIDI Research Group RNM18 from the Junta de Andalucía. The Ministerio de Economía y Competitividad of the Spanish Government funded the study through the research projects CGL2012-40043-C02-02 and CGL2016-80543-P, and a predoctoral grant (BES-2013-063931) to JE. ES was supported by the Fundação para a Ciência ea Tecnologia (Portugal), through the postdoctoral program (SFRH/BPD/96637/2013), and the University of Aveiro (Department of Biology) and FCT/MEC (CESAM RU UID/AMB/50017), co-financed by the FEDER within the PT2020 Partnership Agreement. LR, DD and CC were supported by the Italian National PRIN Program “Genomics and host-pathogen interaction: a study model in the One-Health perspective

A highly endemic area of Echinococcus multilocularis identified through a comparative re-assessment of prevalence in the red fox (Vulpes vulpes), Alto Adige (Italy: 2019-2020)

Surveillance of Echinococcus multilocularis at the edge of its range is hindered by fragmented distributional patterns and low prevalence in definitive hosts. Thus, tests with adequate levels of sensitivity are especially important for discriminating between infected and non-infected areas. In this study we reassessed the prevalence of E. multilocularis at the southern border of its distribution in Province of Bolzano (Alto Adige, northeastern Alps, Italy), to improve surveillance in wildlife and provide more accurate estimates of exposure risk. We compared the diagnostic test currently implemented for surveillance based on coproscopy and multiplex PCR (CMPCR) to a real-time quantitative PCR (qPCR) in 235 fox faeces collected in 2019 and 2020. The performances of the two tests were estimated using a scraping technique (SFCT) applied to the small intestines of a subsample (n = 123) of the same foxes as the reference standard. True prevalence was calculated and the sample size required by each faecal test for the detection of the parasite was then estimated. True prevalence of E. multilocularis in foxes (14.3%) was markedly higher than reported in the last decade, which was never more than 5% from 2012 to 2018 in the same area. In addition, qPCR showed a much higher sensitivity (83%) compared to CMPCR (21%) and agreement with the reference standard was far higher for qPCR (0.816) than CMPCR (0.298) meaning that for the latter protocol, a smaller sample size would be required to detect the disease. Alto Adige should be considered a highly endemic area. Routine surveillance on definitive hosts at the edges of the E. multilocularis distribution should be applied to smaller geographic areas, and rapid, sensitive diagnostic tools using directly host faeces, such as qPCR, should be adopted

The effects of antibiotic additions to extenders on fresh and frozen–thawed bull semen

Reproduction in dairy cows is based around the use of cryopreserved semen. Antibiotics are utilized to control bacterial contamination and growth in cryopreserved bull semen. The antibiotic resistance of some bacteria required the evaluation of new antibiotic combinations with a high level of antibacterial effectiveness and a negligible effect on spermatozoa. In this research, we studied the effect of the fluorinate carboxyquinolone ofloxacin and the combination of ceftiofur/tylosin on bull spermatozoa and in-field bacterial growth. In Experiment 1, the toxicity of different levels of ofloxacin and ceftiofur/tylosin was tested by the incubation of bull spermatozoa and the evaluation of sperm kinetic parameters, membranes and acrosome integrity after dilution, and at 60 and 120 min after incubation. The data reported in this study reveals that both antibiotic combinations, at all concentrations, seem to have a negligible effect on spermatozoa with respect to all of the parameters examined (p>0.05). Furthermore, progressive motility was significantly higher for sperm diluted with both antibiotic combinations compared with samples without antibiotics (p<0.01). In Experiment 2, the ability of ofloxacin or ceftiofur/tylosin to control bacterial growth during bovine semen cryopreservation was compared with the combination of gentamicin/tylosin/spectinomycin/lincomycin. A significant reduction in progressive motility was found in cooled semen with respect to all of the antibiotic treatments (p<0.05). However, the membrane integrity was found to significantly rise in frozen samples with, compared to samples without, antibiotics (p<0.05). In a bull, gentamicin, tylosin, spectinomycin, and lincomycin failed to control bacterial growth in the cryopreserved sample, while no such growth was found in samples extended with ceftiofur/tylosin or ofloxacin. In conclusion, both ceftiofur/tylosin and ofloxacin can be safely added to bull seminal extenders, and both can protect insemination doses from bacteria that are resistant to other antibiotic combinations

Evaluation of the use of serological and bacteriological investigation for monitoring and controlling Salmonella in Italian pig herds

At the European level the control of foodborne diseases is defined by the new zoonoses legislation (Directive 20031991EC and Regulation (EC) No 2160/2003), which points out the necessity to establish surveillance programmes for zoonotic agents in animal populations. Recently Commission Decision 2006/6681EC concerning a baseline study on the prevalence of Salmonella in slaughter pigs has been published. Many different strategies have been developed and applied by EU Member States in order to Implement momtoring and/or control programmes for Salmonella in pigs; these strategies are mamly based on bacteriological analysis (performed on caecal content, ileo caecal lymph nodes or carcass swabs collected at slaughterhouse) and/or on serological analysis (mainly performed on meat juice obtained from diaphragm muscle).</p

Antibiotic Use in Alpine Dairy Farms and Its Relation to Biosecurity and Animal Welfare

The quantification of antimicrobial usage (AMU) in food-producing animals can help identify AMU risk factors, thereby enhancing appropriate stewardship policies and strategies for a more rational use. AMU in a sample of 34 farms in the Province of Trento (north-eastern Italy) from 2018 to 2020 was expressed as defined daily doses for animals per population correction unit according to European Surveillance of Veterinary Antimicrobial Consumption guidelines (DDDvet) and according to Italian guidelines (DDDAit). A retrospective analysis was carried out to test the effects of several husbandry practices on AMU. Overall, the average AMU ranged between 6.5 DDDAit in 2018 and 5.2 DDDAit in 2020 (corresponding to 9 and 7 DDDvet, respectively), showing a significant trend of decrement (−21.3%). Usage of the highest priority critically important antimicrobials (HPCIA) was reduced by 83% from 2018 to 2020. Quarantine management, available space, water supply, animals’ cleanliness and somatic cell count had no significant association with AMU. Rather, farms with straw-bedded cubicles had lower AMU levels than those with mattresses and concrete floors (p < 0.05). In conclusion, this study evidenced a decrement in AMU, particularly regarding HPCIA, but only a few risk factors due to farm management

Assessing Red Fox (<i>Vulpes vulpes</i>) Demographics to Monitor Wildlife Diseases: A Spotlight on <i>Echinococcus multilocularis</i>

The assessment of red fox population density is considered relevant to the surveillance of zoonotic agents vectored by this species. However, density is difficult to estimate reliably, since the ecological plasticity and elusive behavior of this carnivore hinder classic methods of inference. In this study, red fox population density was estimated using a non-invasive molecular spatial capture-recapture (SCR) approach in two study areas: one in a known hotspot of the zoonotic cestode Echinococcus multilocularis, and another naïve to the parasite. Parasitological investigations on collected samples confirmed the presence of the parasite exclusively in the former area; the SCR results indicated a higher fox population density in the control area than in the hotspot, suggesting either that the relationship between fox density and parasite prevalence is not linear and/or the existence of other latent factors supporting the parasitic cycle in the known focus. In addition, fox spotlight count data for the two study areas were used to estimate the index of kilometric abundance (IKA). Although this method is cheaper and less time-consuming than SCR, IKA values were the highest in the areas with the lower molecular SCR density estimates, confirming that IKA should be regarded as a relative index only

Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica

Abstract Background Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populations