On-Line Monitoring of Blind Fastener Installation Process

Blind fasteners are of special interest for aircraft construction since they allow working on joints where only one side is accessible, as is the case in many common aerospace box-type structures, such as stabilizers and flaps. This paper aims to deliver an online monitoring method for the detection of incorrect installed blind fasteners. In this type of fastener, the back side of the assembly is not accessible, so monitoring the process installation is suitable as a system to assess the formed head at the back side with no access. The solution proposed consists of an on-line monitoring system that is based on sensor signals acquired during the installation. The signals are conveniently analyzed in order to provide an evaluation outcome on how the fastener was installed. This new method will help production to decrease/eliminate time and cost-intensive inspections and fasteners over installation in structures. The decrease of the number of installed fasteners will also contribute to weight savings and will reduce the use of resources.This research is part of the BLINDFAST: INNOVATIVE BLIND FASTENER MONITORING TECHNOLOGY FOR QUALITY CONTROL project, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement 686827

The role of tissue-resident memory T cells as mediators for response and toxicity in immunotherapy-treated melanoma—two sides of the same coin?

Tissue-resident memory T cells (TRM cells) have become an interesting subject of study for antitumor immunity in melanoma and other solid tumors. In the initial phases of antitumor immunity, they maintain an immune equilibrium and protect against challenges with tumor cells and the formation of primary melanomas. In metastatic settings, they are a prime target cell population for immune checkpoint inhibition (ICI) because they highly express inhibitory checkpoint molecules such as PD-1, CTLA-4, or LAG-3. Once melanoma patients are treated with ICI, TRM cells residing in the tumor are reactivated and expand. Tumor killing is achieved by secreting effector molecules such as IFN-γ. However, off-target effects are also observed. Immune-related adverse events, such as those affecting barrier organs like the skin, can be mediated by ICI-induced TRM cells. Therefore, a detailed understanding of this memory T-cell type is obligatory to better guide and improve immunotherapy regimens

On-Line Monitoring of Blind Fastener Installation Process

Blind fasteners are of special interest for aircraft construction since they allow working on joints where only one side is accessible, as is the case in many common aerospace box-type structures, such as stabilizers and flaps. This paper aims to deliver an online monitoring method for the detection of incorrect installed blind fasteners. In this type of fastener, the back side of the assembly is not accessible, so monitoring the process installation is suitable as a system to assess the formed head at the back side with no access. The solution proposed consists of an on-line monitoring system that is based on sensor signals acquired during the installation. The signals are conveniently analyzed in order to provide an evaluation outcome on how the fastener was installed. This new method will help production to decrease/eliminate time and cost-intensive inspections and fasteners over installation in structures. The decrease of the number of installed fasteners will also contribute to weight savings and will reduce the use of resources.This research is part of the BLINDFAST: INNOVATIVE BLIND FASTENER MONITORING TECHNOLOGY FOR QUALITY CONTROL project, which has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement 686827

Respiration-based investigation of adsorbent-bioprocess compatibility

Background: The efficiency of downstream processes plays a crucial role in the transition from conventional petrochemical processes to sustainable biotechnological production routes. One promising candidate for product separation from fermentations with low energy demand and high selectivity is the adsorption of the target product on hydrophobic adsorbents. However, only limited knowledge exists about the interaction of these adsorbents and the bioprocess. The bioprocess could possibly be harmed by the release of inhibitory components from the adsorbent surface. Another possibility is co-adsorption of essential nutrients, especially in an in situ application, making these nutrients unavailable to the applied microorganism. Results: A test protocol investigating adsorbent-bioprocess compatibility was designed and applied on a variety of adsorbents. Inhibitor release and nutrient adsorption was studied in an isolated manner. Respiratory data recorded by a RAMOS device was used to assess the influence of the adsorbents on the cultivation in three different microbial systems for up to six different adsorbents per system. While no inhibitor release was detected in our investigations, adsorption of different essential nutrients was observed. Conclusion: The application of adsorption for product recovery from the bioprocess was proven to be generally possible, but nutrient adsorption has to be assessed for each application individually. To account for nutrient adsorption, adsorptive product separation should only be applied after sufficient microbial growth. Moreover, concentrations of co-adsorbed nutrients need to be increased to compensate nutrient loss. The presented protocol enables an investigation of adsorbent-bioprocess compatibility with high-throughput and limited effort

A mental model for the solution of the direct and inverse kinematic problem

Steinkühler U, Deitert J, Cruse H. A mental model for the solution of the direct and inverse kinematic problem. In: Proceed. of the ESANN 93. 1993

Kernel density-based pattern classification in blind fasteners installation

Publisher Copyright: © Springer International Publishing AG 2017.In this work we introduce a kernel density-based pattern classification approach for the automatic identification of behavioral patterns from monitoring data related to blind fasteners installation. High density regions are estimated from feature space to establish behavioral patterns, automatically removing outliers and noisy instances in an iterative process. First the kernel density estimator is applied on the fastener features representing the quality of the installation. Then the behavioral patterns are identified from resulting high density regions, also considering the proximity between instances. Patterns are computed as the average of related monitoring torque-rotation diagrams. New fastening installations can be thus automatically classified in an online fashion. In order to show the validity of the approach, experiments have been conducted on real fastening data. Experimental results show an accurate pattern identification and classification approach, obtaining a global accuracy over 78% and improving current detection capabilities and existing evaluation systems.These results are part of a project that has received funding from the European Unions Horizon 2020 research and innovation programme under grant agreement 686827.Peer reviewe

Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates

Abstract Background One critical parameter in microbial cultivations is the composition of the cultivation medium. Nowadays, the application of chemically defined media increases, due to a more defined and reproducible fermentation performance than in complex media. In order, to improve cost-effectiveness of fermentation processes using chemically defined media, the media should not contain nutrients in large excess. Additionally, to obtain high product yields, the nutrient concentrations should not be limiting. Therefore, efficient medium optimization techniques are required which adapt medium compositions to the specific nutrient requirements of microorganisms. Results Since most Paenibacillus cultivation protocols so far described in literature are based on complex ingredients, in this study, a chemically defined medium for an industrially relevant Paenibacillus polymyxa strain was developed. A recently reported method, which combines a systematic experimental procedure in combination with online monitoring of the respiration activity, was applied and extended to identify growth limitations for Paenibacillus polymyxa. All cultivations were performed in microtiter plates. By systematically increasing the concentrations of different nutrient groups, nicotinic acid was identified as a growth-limiting component. Additionally, an insufficient buffer capacity was observed. After optimizing the growth in the chemically defined medium, the medium components were systematically reduced to contain only nutrients relevant for growth. Vitamins were reduced to nicotinic acid and biotin, and amino acids to methionine, histidine, proline, arginine, and glutamate. Nucleobases/-sides could be completely left out of the medium. Finally, the cultivation in the reduced medium was reproduced in a laboratory fermenter. Conclusion In this study, a reliable and time-efficient high-throughput methodology was extended to investigate limitations in chemically defined media. The interpretation of online measured respiration activities agreed well with the growth performance of samples measured in parallel via offline analyses. Furthermore, the cultivation in microtiter plates was validated in a laboratory fermenter. The results underline the benefits of online monitoring of the respiration activity already in the early stages of process development, to avoid limitations of medium components, oxygen limitation and pH inhibition during the scale-up