Current desires of conspecific observers affect cache-protection strategies in California scrub-jays and Eurasian jays

Many corvid species accurately remember the locations where they have seen others cache food, allowing them to pilfer these caches efficiently once the cachers have left the scene [1] . To protect their caches, corvids employ a suite of different cache-protection strategies that limit the observers’ visual or acoustic access to the cache site [2,3] . In cases where an observer’s sensory access cannot be reduced it has been suggested that cachers might be able to minimise the risk of pilfering if they avoid caching food the observer is most motivated to pilfer [4] . In the wild, corvids have been reported to pilfer others’ caches as soon as possible after the caching event [5] , such that the cacher might benefit from adjusting its caching behaviour according to the observer’s current desire. In the current study, observers pilfered according to their current desire: they preferentially pilfered food that they were not sated on. Cachers adjusted their caching behaviour accordingly: they protected their caches by selectively caching food that observers were not motivated to pilfer. The same cache-protection behaviour was found when cachers could not see on which food the observers were sated. Thus, the cachers’ ability to respond to the observer’s desire might have been driven by the observer’s behaviour at the time of caching.We thank the BBSRC, the ERC and the Leverhulme Trust for funding the research. K.F.B., F.L. and C.D. were funded by ERASMUS scholarships

Attempts to detect retrotransposition and de novo deletion of Alus and other dispersed repeats at specific loci in the human genome

Dispersed repeat elements contribute to genome instability by de novo insertion and unequal recombination between repeats. To study the dynamics of these processes, we have developed single DNA molecule approaches to detect de novo insertions at a single locus and Alu-mediated deletions at two different loci in human genomic DNA. Validation experiments showed these approaches could detect insertions and deletions at frequencies below 10(-6) per cell. However, bulk analysis of germline (sperm) and somatic DNA showed no evidence for genuine mutant molecules, placing an upper limit of insertion and deletion rates of 2 x 10(-7) and 3 x 10(-7), respectively, in the individuals tested. Such re-arrangements at these loci therefore occur at a rate lower than that detectable by the most sensitive methods currently available

Last Interglacial Climate in Northern Sweden—Insights from a Speleothem Record

Continental records with absolute dates of the timing and progression of climatic conditions during the Last Interglacial (LIG) from northern Europe are rare. Speleothems from northern Europe have a large potential as archives for LIG environmental conditions since they were formed in sheltered environments and may be preserved beneath ice sheets. Here, we present δ13C and δ18O values from speleothem Kf-21, from Korallgrottan in Jämtland (northwest Sweden). Kf-21 is dated with five MC-ICPMS U-Th dates with errors smaller than ~1 ka. Kf-21 started forming at ~130.2 ka and the main growth phase with relatively constant growth rates lasted from 127.3 ka to 124.4 ka, after which calcite formation ceased. Both δ13C and δ18O show rapid shifts but also trends, with a range of values within their Holocene counterparts from Korallgrottan. Our results indicate an early onset of the LIG in northern Europe with ice-free conditions at ~130 ka. Higher growth rates combined with more negative δ18O values between ~127.3 and 126.8 ka, interpreted here as warmer and more humid conditions, as well as indications of a millennial-scale cold spell centered at 126.2 ka, resemble findings from speleothem records from other parts of Europe, highlighting that these were regional scale climatic patterns

Last Interglacial Climate in Northern Sweden—Insights from a Speleothem Record

Continental records with absolute dates of the timing and progression of climatic conditions during the Last Interglacial (LIG) from northern Europe are rare. Speleothems from northern Europe have a large potential as archives for LIG environmental conditions since they were formed in sheltered environments and may be preserved beneath ice sheets. Here, we present δ13C and δ18O values from speleothem Kf-21, from Korallgrottan in Jämtland (northwest Sweden). Kf-21 is dated with five MC-ICPMS U-Th dates with errors smaller than ~1 ka. Kf-21 started forming at ~130.2 ka and the main growth phase with relatively constant growth rates lasted from 127.3 ka to 124.4 ka, after which calcite formation ceased. Both δ13C and δ18O show rapid shifts but also trends, with a range of values within their Holocene counterparts from Korallgrottan. Our results indicate an early onset of the LIG in northern Europe with ice-free conditions at ~130 ka. Higher growth rates combined with more negative δ18O values between ~127.3 and 126.8 ka, interpreted here as warmer and more humid conditions, as well as indications of a millennial-scale cold spell centered at 126.2 ka, resemble findings from speleothem records from other parts of Europe, highlighting that these were regional scale climatic patterns

Meeting Report for Mobile DNA 2010

An international conference on mobile DNA was held 24-28 April 2010 in Montreal, Canada. Sponsored by the American Society for Microbiology, the conference's goal was to bring together researchers from around the world who study transposition in diverse organisms using multiple experimental approaches. The meeting drew over 190 attendees and most contributed through poster presentations, invited talks and short talks selected from poster abstracts. The talks were organized into eight scientific sessions, which ranged in topic from the evolutionary dynamics of mobile genetic elements to transposition reaction mechanisms. Here we present highlights from the platform sessions with a focus on talks presented by the invited speakers

Identification and Characterization of AES-135, a Hydroxamic Acid-Based HDAC Inhibitor That Prolongs Survival in an Orthotopic Mouse Model of Pancreatic Cancer

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive, incurable cancer with a 20% 1 year survival rate. While standard-of-care therapy can prolong life in a small fraction of cases, PDAC is inherently resistant to current treatments, and novel therapies are urgently required. Histone deacetylase (HDAC) inhibitors are effective in killing pancreatic cancer cells in in vitro PDAC studies, and although there are a few clinical studies investigating combination therapy including HDAC inhibitors, no HDAC drug or combination therapy with an HDAC drug has been approved for the treatment of PDAC. We developed an inhibitor of HDACs, AES-135, that exhibits nanomolar inhibitory activity against HDAC3, HDAC6, and HDAC11 in biochemical assays. In a three-dimensional coculture model, AES-135 kills low-passage patient-derived tumor spheroids selectively over surrounding cancer-associated fibroblasts and has excellent pharmacokinetic properties in vivo. In an orthotopic murine model of pancreatic cancer, AES-135 prolongs survival significantly, therefore representing a candidate for further preclinical testing

Environmental Costs of Government-Sponsored Agrarian Settlements in Brazilian Amazonia

Brazil has presided over the most comprehensive agrarian reform frontier colonization program on Earth, in which ~1.2 million settlers have been translocated by successive governments since the 1970's, mostly into forested hinterlands of Brazilian Amazonia. These settlements encompass 5.3% of this ~5 million km2 region, but have contributed with 13.5% of all land conversion into agropastoral land uses. The Brazilian Federal Agrarian Agency (INCRA) has repeatedly claimed that deforestation in these areas largely predates the sanctioned arrival of new settlers. Here, we quantify rates of natural vegetation conversion across 1911 agrarian settlements allocated to 568 Amazonian counties and compare fire incidence and deforestation rates before and after the official occupation of settlements by migrant farmers. The timing and spatial distribution of deforestation and fires in our analysis provides irrefutable chronological and spatially explicit evidence of agropastoral conversion both inside and immediately outside agrarian settlements over the last decade. Deforestation rates are strongly related to local human population density and road access to regional markets. Agrarian settlements consistently accelerated rates of deforestation and fires, compared to neighboring areas outside settlements, but within the same counties. Relocated smallholders allocated to forest areas undoubtedly operate as pivotal agents of deforestation, and most of the forest clearance occurs in the aftermath of government-induced migration

Regions identity between the genome of vertebrates and non-retroviral families of insect viruses

<p>Abstract</p> <p>Background</p> <p>The scope of our understanding of the evolutionary history between viruses and animals is limited. The fact that the recent availability of many complete insect virus genomes and vertebrate genomes as well as the ability to screen these sequences makes it possible to gain a new perspective insight into the evolutionary interaction between insect viruses and vertebrates. This study is to determine the possibility of existence of sequence identity between the genomes of insect viruses and vertebrates, attempt to explain this phenomenon in term of genetic mobile element, and try to investigate the evolutionary relationship between these short regions of identity among these species.</p> <p>Results</p> <p>Some of studied insect viruses contain variable numbers of short regions of sequence identity to the genomes of vertebrate with nucleotide sequence length from 28 bp to 124 bp. They are found to locate in multiple sites of the vertebrate genomes. The ontology of animal genes with identical regions involves in several processes including chromatin remodeling, regulation of apoptosis, signaling pathway, nerve system development and some enzyme-like catalysis. Phylogenetic analysis reveals that at least some short regions of sequence identity in the genomes of vertebrate are derived the ancestral of insect viruses.</p> <p>Conclusion</p> <p>Short regions of sequence identity were found in the vertebrates and insect viruses. These sequences played an important role not only in the long-term evolution of vertebrates, but also in promotion of insect virus. This typical win-win strategy may come from natural selection.</p