3D fluid-dynamic ovarian cancer model resembling systemic drug administration for efficacy assay.

Recently, 3D in vitro cancer models have become important alternatives to animal tests for establishing the efficacy of anticancer treatments. In this work, 3D SKOV-3 cell-laden alginate hydrogels were established as ovarian tumor models and cultured within a fluid-dynamic bioreactor (MIVO®) device able to mimic the capillary flow dynamics feeding the tumor. Cisplatin efficacy tests were performed within the device over time and compared with (i) the in vitro culture under static conditions and (ii) a xenograft mouse model with SKOV-3 cells, by monitoring and measuring cell proliferation or tumor regression, respectively, over time. After one week of treatment with 10 µM cisplatin, viability of cells within the 3D hydrogels cultured under static conditions remained above 80%. In contrast, the viability of cells within the 3D hydrogels cultured within dynamic MIVO® decreased by up to 50%, and very few proliferating Ki67-positive cells were observed through immunostaining. Analysis of drug diffusion, confirmed by computational analysis, explained that these results are due to different cisplatin diffusion mechanisms in the two culture conditions. Interestingly, the outcome of the drug efficacy test in the xenograft model was about 44% of tumor regression after 5 weeks, as predicted in a shorter time just in the fluid-dynamic in vitro tests carried out within MIVO® device. These results indicate that the in vivo-like dynamic environment provided by the MIVO® device allows to better model the 3D tumor environment and predict in vivo drug efficacy than a static in vitro model

Discrete element simulation of the charge in the hopper of a blast furnace, calibrating the parameters through an optimization algorithm

The purpose of this study is to simulate the distribution of a coarse granular material discharged in a hopper via a conveyor belt. This simulation is intended to be a model calibration for an optimization that will be later performed to obtain a proper material distribution device. From the hopper, the material is discharged in a blast furnace. Hence, achieving an adequate distribution in the hopper is crucial, since that distribution is directly linked to how the material spreads in the blast furnace, and this heavily influences the efficiency of the whole steel-making process. The apparatus is modeled by online three dimensional Computer-Aided Design software Onshape. Rocky DEM, a Computer-Aided Engineering software based on Discrete Element Method, is used to simulate the charge. The parameters of the numerical model are calibrated through an optimization algorithm. This phase is realized thanks to the optimization platform modeFRONTIER, using an algorithm that exploits meta-models to reduce the computational time of the optimization. By comparing the simulated results with the visual data obtained from blast furnace plant, the goal is to validate the model and to better understand the behavior of the whole charging process

40. Premio Sergio Amidei : catalogo 2021

Catalogo della 40a edizione del Premio Internazionale alla Migliore Sceneggiatura Cinematografica "Sergio Amidei" tenutasi a Gorizia dal 23 al 29 luglio 2021