1,419 research outputs found
No evidence of a significant role for CTLA-4 in multiple sclerosis
Variation in the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) gene plays a significant role in determining susceptibility to autoimmune thyroid disease and type 1 diabetes. Its role in multiple sclerosis is more controversial. In order to explore this logical candidate more thoroughly, we genotyped 771 multiple sclerosis trio families from the United Kingdom for the 3? untranslated region variable number tandem repeat, the CT60 single nucleotide polymorphism (SNP) and five haplotype-tagging SNPs. No individual marker or common haplotype showed evidence of association with disease. These data suggest that any effect of CTLA-4 on multiple sclerosis susceptibility is likely to be very small
Manufacturing Cells for Clinical Use
Citation: Weiss, M. L., Rao, M. S., Deans, R., & Czermak, P. (2016). Manufacturing Cells for Clinical Use. Stem Cells International, 5. doi:10.1155/2016/1750697The growth in the number of registered clinical trials indicates that there is a need for cells for many types of cell therapy. Figure 1, which is reprinted from the excellent blog maintained by Alexi Bersenev, shows that the cell type used in most clinical trials worldwide is the mesenchymal stromal cell (MSC). The MSC type requires in vitro expansion to reach a clinical dose and thus there is a desire to optimize and standardize processes and procedures for MSC manufacture specifically for clinical use
Convergence and segregation of the multiple rod pathways in mammalian retina.
Using a multidisciplinary approach, we demonstrate that three different pathways are responsible for the transmission of rod signals across the mouse retina. Each pathway serves a primarily nonoverlapping range of stimulus intensities, with ganglion cells receiving either segregated or convergent inputs. For both on-center (ON) and off-center (OFF) ganglion cells, the primary rod pathway carries signals with the lowest threshold, whereas the secondary rod pathway is less sensitive by approximately 1 log unit. In addition, OFF signaling uses a tertiary rod pathway that is approximately 1 log unit less sensitive than the secondary. Although some ganglion cells received rod inputs exclusively from one of the pathways, others showed convergent inputs. Using pharmacological and genetic approaches, we defined classes of ON and OFF ganglion cells for which the scotopic inputs derive only from the primary pathway or from both primary and secondary pathways. In addition, we observed a class of OFF ganglion cell receiving mixed input from primary and tertiary pathways. Interestingly, OFF ganglion cells receiving convergent inputs from all three rod pathways or from the secondary and tertiary pathways together were never observed. Overall, our data show a complex arrangement of convergence and segregation of rod inputs to ganglion cells in the mammalian retina
Tomographic approach to resolving the distribution of LISA Galactic binaries
The space based gravitational wave detector LISA is expected to observe a
large population of Galactic white dwarf binaries whose collective signal is
likely to dominate instrumental noise at observational frequencies in the range
10^{-4} to 10^{-3} Hz. The motion of LISA modulates the signal of each binary
in both frequency and amplitude, the exact modulation depending on the source
direction and frequency. Starting with the observed response of one LISA
interferometer and assuming only doppler modulation due to the orbital motion
of LISA, we show how the distribution of the entire binary population in
frequency and sky position can be reconstructed using a tomographic approach.
The method is linear and the reconstruction of a delta function distribution,
corresponding to an isolated binary, yields a point spread function (psf). An
arbitrary distribution and its reconstruction are related via smoothing with
this psf. Exploratory results are reported demonstrating the recovery of binary
sources, in the presence of white Gaussian noise.Comment: 13 Pages and 9 figures high resolution figures can be obtains from
http://www.phys.utb.edu/~rajesh/lisa_tomography.pd
Quantum reconstruction of an intense polarization squeezed optical state
We perform a reconstruction of the polarization sector of the density matrix
of an intense polarization squeezed beam starting from a complete set of Stokes
measurements. By using an appropriate quasidistribution, we map this onto the
Poincare space providing a full quantum mechanical characterization of the
measured polarization state.Comment: 4 pages, 4 eps color figure
Thermal Stability of RP-2 for Hydrocarbon Boost Regenerative Cooling
A series of tests were performed in the NASA Glenn Research Centers Heated Tube Facility to study the heat transfer and thermal stability behavior of RP-2 under conditions similar to those found in rocket engine cooling channels. It has long been known that hydrocarbon fuels, such as RP-2, can decompose at high temperature to form deposits (coke) which can adversely impact rocket engine cooling channel performance. The heated tube facility provides a simple means to study these effects. Using resistively heated copper tubes in a vacuum chamber, flowing RP-2 was heated to explore thermal effects at a range of test conditions. Wall temperature (850-1050F) and bulk fluid temperature (300-500F) were varied to define thermal decomposition and stability at each condition. Flow velocity and pressure were fixed at 75 fts and 1000 psia, respectively. Additionally, five different batches of RP-2 were tested at identical conditions to examine any thermal stability differences resulting from batch to batch compositional variation. Among these tests was one with a potential coke reducing additive known as 1,2,3,4-Tetrahydroquinoline (THQ). While copper tubes were used for the majority of tests, two exploratory tests were performed with a copper alloy known as GRCop-42. Each tube was instrumented with 15 thermocouples to examine the temperature profile, and carbon deposition at each thermocouple location was determined post-test in an oxidation furnace. In many tests, intermittent local temperature increases were observed visually and in the thermocouple data. These hot spots did not appear to correspond with a higher carbon deposition
Tissue accumulation of cephalothin in burns: A comparative study by microdialysis of subcutaneous interstitial fluid cephalothin concentrations in burn patients and healthy volunteers
Burn tissue sites are a potential source of bacteremia during debridement surgery. Burn injury is likely to affect the distribution of antibiotics to tissues, but direct evidence of this is lacking. The aim of this study was to directly evaluate the influence of burn trauma on the distribution of cephalothin to peripheral tissues. We used subcutaneous microdialysis techniques to monitor interstitial fluid concentrations of cephalothin in the burnt and nonburnt tissues of adult patients with severe burns following parenteral administration of 1 g cephalothin for surgical prophylaxis. Analogous simultaneous studies conducted with healthy adult volunteers provided reference tissue concentration data. Equivalent tissue exposures were seen for burn and nonburn sites, giving overall median interstitial cephalothin concentrations (from 0 to 240 min) of 2.84 mg/liter and 3.06 mg/liter, respectively. A lower overall median interstitial cephalothin concentration of 0.54 mg/liter was observed for healthy individuals, and the patient nonburnt tissue and volunteer control tissue cephalothin concentrations exhibited significantly different data distributions (P < 0.001; Kolmogorov-Smirnov nonparametric test). The duration of tissue residence for cephalothin was longer for burn patients than for healthy volunteers. The results demonstrate the potential fallibility of using healthy population models to extrapolate tissue pharmacodynamic predictions from plasma data for burn patients
Fermiology via the electron momentum distribution
Investigations of the Fermi surface via the electron momentum distribution
reconstructed from either angular correlation of annihilation radiation (or
Compton scattering) experimental spectra are presented. The basis of these
experiments and mathematical methods applied in reconstructing
three-dimensional densities from line (or plane) projections measured in these
experiments are described. The review of papers where such techniques have been
applied to study the Fermi surface of metallic materials with showing their
main results is also done.Comment: 22 pages, 9 Figures, 4 Table
The dark kinase STK32A regulates hair cell planar polarity opposite of EMX2 in the developing mouse inner ear.
The vestibular maculae of the inner ear contain sensory receptor hair cells that detect linear acceleration and contribute to equilibrioception to coordinate posture and ambulatory movements. These hair cells are divided between two groups, separated by a line of polarity reversal (LPR), with oppositely oriented planar-polarized stereociliary bundles that detect motion in opposite directions. The transcription factor EMX2 is known to establish this planar polarized organization in mouse by regulating the distribution of the transmembrane receptor GPR156 at hair cell boundaries in one group of cells. However, the genes regulated by EMX2 in this context were previously not known. Using mouse as a model, we have identified the serine threonine kinase STK32A as a downstream effector negatively regulated by EMX2
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