Bacchilide e il Ditirambo

La tesi inquadra i Ditirambi di Bacchilide all'interno della storia evolutiva di un genere dai caratteri complessi e sfuggenti agli studiosi moderni, anche a causa della scarsità di testi a disposizione. La prima parte traccia l'evoluzione del ditirambo, dalle antiche e pressoché oscure origini di canto rituale connesso con il culto di Dioniso, allo sviluppo di una particolare forma lirica corale, dotata di sue specifiche caratteristiche e funzionalità artistiche. La seconda parte analizza poi singolarmente i sei ditirambi bacchilidei riportati dal famoso papiro londinese 733, tenendo conto delle varie problematiche formali e contenutistiche proposte dal testo; l'obiettivo dell'analisi è quello di recuperare di volta in volta il senso della composizione dell'ode e, se possibile, l'originaria intenzione espressiva dell'autore, collocando lo specifico componimento nella luce dello stretto rapporto di interdipendenza tra genere e occasione della rappresentazione

Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period

High neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular, advanced oxidation protein products (AOPP), are OS biomarkers linked to PMN activity. To test this hypothesis, the relationship between protein oxidation and uterus health was studied in thirty-eight dairy cows during the puerperium. The animals were found to be cycling, without any signs of disease and pharmacological treatments. PMN count was performed either through a cytobrush or a uterine horn lavage (UHL). Cows were classified into four groups, based on the uterine ultrasonographic characteristics and the PMN percentage in the uterine horns with a higher percentage of high neutrophil horn (HNH). They were classified as: Healthy (H); Subclinical Endometritis (SCE); Grade 1 Endometritis (EM1); and Grade 2 Endometritis (EM2). AOPP and carbonyls were measured in plasma and UHL. UHL samples underwent Western blot analysis to visualize the carbonyl and dityrosine formation. Plasma AOPP were higher (p < 0.05) in EM2. AOPP and carbonyl group concentrations were higher in the HNH samples (p < 0.05). Protein concentration in the UHL was higher in the EM2 (p < 0.05). Carbonyl and dityrosine formation was more intense in EM1 and EM2. Protein oxidation observed in the EM2 suggests the presence of an inflammatory status in the uterus which, if not adequately hindered, could result in low fertility

Effect of auxotrophies on yeast growth in aerated fed-batch reactor

Mutant and deletion strains of the yeast Saccharomyces cerevisiae having one/several auxotrophies are largely used in the development of recombinant strains for heterologous protein production because they ensure maintenance of plasmids with selectable markers. The production is usually carried out by culturing the recombinant strain in aerated fed-batch, where sugar limitation achieves high yields of biomass and product. In a previous work, it was evidenced that growth of the auxotrophic S. cerevisiae BY4741 (MATa, ura30, leu20, met150, his31) engineered for human IL-1 production, and employed in aerated fed-batch, early arrested even in the presence of a correct nutritional complementation (being specific nutrients for genetically uncomplemented auxotrophies provided in no growthlimiting amounts). It was assumed that this behaviour may depend on the high number of auxotrophies, since the prototrophic S288C, from which BY4741 derives, showed a typical performance under the same cultivation mode. Therefore, a systematic investigation on the effect of auxotrophies on yeast growth in aerated fed-batch was carried out. Four isogenic strains of the CEN.PK family, with a progressively increasing number of auxotrophies (from one to four) were assayed under fed-batch conditions and a proper nutritional complementation. Feeding to the reactor was exponentially increased imposing a specific growth rate below the critical one. The behaviour of the auxotrophic strains was compared with that of the isogenic prototrophic strain. by evaluating the capacity to keep the specific growth rate chosen. A clear correlation among optimum growth and number of auxotrophies has been found. Furthermore we have investigated the possible effect of the type of auxotrophy (ura- or leu-) on the strain performance, monitoring as well cell viability of each strain. The study is a contribution to know the phenotypic effects of auxotrophies in yeast and can have implications for biotechnological applications

Effect of auxotrophies on yeast performance in aerated fed-batch reactor

A systematic investigation on the effects of auxotrophies on the performance of yeast in aerated fed-batch reactor was carried out. Six isogenic strains from the CEN.PK family of Saccharomyces cerevisiae,one prototroph and five auxotrophs, were grown in aerated fed-batch reactor using the same operative conditions and a proper nutritional supplementation. The performance of the strains, in terms of final biomass decreased with increasing the number of auxotrophies. Auxotrophy for leucine exerted a profound negative effect on the performance of the strains. Accumulation of reactive oxygen species (ROS) in the cells of the strain carrying four auxotrophies and its significant viability loss, were indicative of an oxidative stress response induced by exposure of cells to the environmental conditions. The mathematical model was fundamental to highlight how the carbon flux, depending on the number and type of auxotrophies, was diverted towards the production of increasingly large quantities of energy for maintenance

Clues of Post-seismic Relaxation for the 1915 Fucino Earthquake (Central Italy) from Modeling of Leveling Data

The 1915 Fucino earthquake (Ms=6.9) was one of the largest and most destructive events in Italy during the last century. The epicentral area is centered in the Abruzzi region (Central Italy), where a long historical record of large earthquakes is available. Seismotectonic studies on this region, based on instrumental seismicity (focal mechanism solutions of major events and stress analysis of background seismicity), borehole break-out studies and several geological and paleoseismological investigations, suggest NE-SW oriented active extension. The 1915 earthquake fault produced detectable surface ruptures for about 20 km along NW-SE striking SW-dipping structures. Coseismic geodetic data recorded in the epicentral area have been inverted in the past (Amoruso et al. 1998 and references therein), indicating a source fault dipping at moderate angle toward SW and a normal focal mechanism, with a non-negligible left-lateral component. Three high precision leveling lines located in a wide sector north and east of the Fucino plain were measured in 1950 and 1997-2000 by the IGM (Istituto Geografico Militare). Two consecutive lines run in a NW-SE direction along the chain, and form a "T-shape" net together with a third line SW-NE striking, towards the Adriatic sea. The total length is about 360 km with a mean benchmark density higher than 0.5 bm/km. The relative elevation changes recorded during this time interval show maximum values between 7 and 12 cm with a signal wavelength of 40-70 km. The observed elevation changes stand significantly above the calculated total error of 1.13 mm sqrt(L) km. A sharp gradient has been observed east of the earthquake epicenter, where we observe peculiar elevation changes along a 40 km long section of the leveling line. The observed elevation changes in Fucino earthquake area seem to comprise both regional tectonic deformation and post-seismic relaxation. The former and the latter effects are expected to dominate along sections of the leveling lines which are respectively about perpendicular and parallel to the Apennines. Since we compare measurements performed in 1950 and 1997-2000, relaxation effects refer to a late stage of the process. We have used Pollitz (1997) code for computing gravitational-viscoelastic postseismic relaxation on a layered spherical Earth. Different Earth models, characterized by different thicknesses and viscosities of crustal layers and of the upper mantle, have been considered. Even if S/N ratio of expected post-seismic effects is not high, comparison between predictions and observations allows to constrain regional crustal structure. Best-fit seismic moment is in good agreement with Amoruso et al. (1998) and residuals are fully consistent with expected regional tectonic deformation in central Apennines

Small leucine rich proteoglycans are differently distributed in normal and pathological endometrium

BACKGROUND: During the woman's fertile period, the non-pregnant uterus is subject to constant cyclic changes. The complex mechanisms that control the balance among proliferation, differentiation, cell death and the structural remodeling of the extracellular matrix can contribute to the benign or malignant endometrial pathological state. The small leucine-rich proteoglycans (SLRPs) are important components of cell surface and extracellular matrices. MATERIALS AND METHODS: Using immunohistochemistry, we showed that the distribution patterns of SLRPs were completely modified in the pathological compared to normal endometrium. RESULTS: The expression of SLRPs was low/absent in all endometrial pathologies examined compared to normal endometrium. We observed an increase of lumican from proliferative to secretory phase of the endometrium and a decrease of fibromodulin, biglycan and decorin. In menopause endometrial tissue, the level of expression of fibromodulin, biglycan, decorin and lumican dramatically decreased. CONCLUSION: The results revealed the prominence and importance of proteoglycans in the tissue architecture and extracellular matrix organization

Therapeutic Efficacy of the Novel Stimuli-Sensitive Nano-Ferritins Containing Doxorubicin in a Head and Neck Cancer Model

Doxorubicin is employed alone or in combination for the treatment of several hematological and solid malignancies; despite its efficacy, there are associated cardiotoxicity limits both in its application in patients with heart disease risk factors and also in its long-term use. HFt-MP-PAS40 is a genetically engineered human ferritin heavy chain (HFt)-based construct able to efficiently entrap and deliver doxorubicin to cancer cells. HF-MP-PAS contains a short motif sequence (defined as MP) responsive to proteolytic cleavage by tumor matrix metalloproteases (MMPs), located between each HFt subunit and a masking polypeptide sequence rich in proline (P), alanine (A), and serine (S) residues (PAS). This carrier displayed excellent therapeutic efficacy in a xenogenic pancreatic cancer model in vivo, leading to a significant increase in overall animal survival in treated mice. Herein, we describe the HFt-MP-PAS40-Dox efficacy against squamous cell carcinomas of the head and neck (HNSCC) with the goal of validating the application of our nano-drug for the treatment of different solid tumors. In addition, a tolerability study in healthy mice was also performed. The results indicate that HFt-MP-PAS40-Dox produced increased anti-tumor effects both in vitro and in vivo in comparison to the free drug in several HNSCC cell lines. In the acute toxicity studies, the maximum tolerated dose (MTD) of HFt-MP-PAS40-Dox was about 3.5 higher than the free drug: 25 mg/kg versus 7 mg/kg doxorubicin equivalents. Importantly, evaluation of heart tissues provided evidence that doxorubicin is less cardio-toxic when encapsulated inside the ferritin carrier. In conclusion, HFt-MP-PAS40-Dox may be administered safely at higher doses compared with the free drug, resulting in superior efficacy to control HNSCC malignancies

Phonons and structures of tetracene polymorphs at low temperature and high pressure

Crystals of tetracene have been studied by means of lattice phonon Raman spectroscopy as a function of temperature and pressure. Two different phases (polymorphs I and II) have been obtained, depending on sample preparation and history. Polymorph I is the most frequently grown phase, stable at ambient conditions. A pressure induced phase transition, observed above 1 GPa, leads to polymorph II, which is also obtained at temperatures below 140 K. Polymorph II can also be maintained at ambient conditions. We have calculated the crystallographic structures and phonon frequencies as a function of temperature, starting from the configurations of the energy minima found by exploring the potential energy surface of crystalline tetracene. The spectra calculated for the first and second deepest minima match satisfactorily those measured for polymorphs I and II, respectively. All published x-ray structures, once assigned to the appropriate polymorph, are also reproduced.Comment: 8 pages, 5 figures, RevTeX4, update after referees report