Chapter 6: Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique

Transient receptor potential (TRP) ion channels are involved in a variety of fundamental physiological processes, and their malfunction produces numerous human diseases. Therefore, these proteins represent a class of attractive drug targets and a class of important off-targets for in vitro pharmacological profiling. In the past decades, the rapid progress in emerging functional assays and instrumentation has enabled to readily monitor thermoTRP channel activity, and to develop high throughput screening (HTS) assays for TPR drug discovery. Chronologically, functional methods for ion channels include the ligand binding assay, flux-based assay, electrophysiology, fluorescence-based assays, and, more recently, automated electrophysiological assays. Here we described the methodology used to monitor the functionality of two thermoTRPs, TRPV1 and TRPM8, based on Ca2+ microfluorography using a 96-well fluorescence plate reader that allows the implementation of a medium- to high-throughput format ideal for drug screening

Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression

TRPM8 is a non-selective cation channel expressed in primary sensory neurons and other tissues, including the prostate and urothelium. Its participation in different physiological and pathological processes such as thermoregulation, pain, itch, inflammation and cancer has been widely described, making it a promising target for therapeutic approaches. The detection and quantification of TRPM8 seems crucial for advancing the knowledge of the mechanisms underlying its role in these pathophysiological conditions. Antibody-based techniques are commonly used for protein detection and quantification, although their performance with many ion channels, including TRPM8, is suboptimal. Thus, the search for reliable antibodies is of utmost importance. In this study, we characterized the performance of six TRPM8 commercial antibodies in three immunodetection techniques: Western blot, immunocytochemistry and immunohistochemistry. Different outcomes were obtained for the tested antibodies; two of them proved to be successful in detecting TRPM8 in the three approaches while, in the conditions tested, the other four were acceptable only for specific techniques. Considering our results, we offer some insight into the usefulness of these antibodies for the detection of TRPM8 depending on the methodology of choice

Medición de la actitud de los aspirantes a docentes en el examen de oposición a cátedra de una universidad pública

La presente investigación tiene como objetivo identificar el tipo de actitud de los aspirantes a maestros a través de las variables independientes, dominio del tema, comunicación, empatía y presencia, en una Universidad Pública del Estado de Nuevo León, México, con el propósito de ser utilizada en los procesos de contratación y selección. Se aplicó un cuestionario conformado por 23 reactivos validados a través del alpha de cronbach a una población de 36 maestros que conformaron el jurado para calificar a los sustentantes. Se realizó la regresión lineal para ver la significancia de cada una de las variables independientes y los resultados obtenidos fueron satisfactorios. La Universidad, como institución de educación superior, requiere docentes altamente calificados para formar profesionales autónomos, creativos y comprometidos con su entorno y con la sociedad en general. ABSTRACT This research aims to identify the kind of attitude of aspiring teachers through independent variables, mastery, communication, empathy and presence in a Public University of Nuevo Leon, Mexico, in order to be used in the processes of recruitment and selection. A questionnaire which was composed of 23 items validated through Cronbach's alpha is applied a population of 36 teachers who formed the jury to assess prospective teachers. Linear regression was performed to check the significance of each of the independent variables and the results obtained were satisfactory. The University as an institution of higher education, requires highly qualified teachers to form autonomous, creative and committed to their professional environment and society in genera

Comprehensive description of clinical characteristics of a large systemic Lupus Erythematosus Cohort from the Spanish Rheumatology Society Lupus Registry (RELESSER) with emphasis on complete versus incomplete lupus differences

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by multiple organ involvement and pronounced racial and ethnic heterogeneity. The aims of the present work were (1) to describe the cumulative clinical characteristics of those patients included in the Spanish Rheumatology Society SLE Registry (RELESSER), focusing on the differences between patients who fulfilled the 1997 ACR-SLE criteria versus those with less than 4 criteria (hereafter designated as incomplete SLE (iSLE)) and (2) to compare SLE patient characteristics with those documented in other multicentric SLE registries. RELESSER is a multicenter hospital-based registry, with a collection of data from a large, representative sample of adult patients with SLE (1997 ACR criteria) seen at Spanish rheumatology departments. The registry includes demographic data, comprehensive descriptions of clinical manifestations, as well as information about disease activity and severity, cumulative damage, comorbidities, treatments and mortality, using variables with highly standardized definitions. A total of 4.024 SLE patients (91% with ≥4 ACR criteria) were included. Ninety percent were women with a mean age at diagnosis of 35.4 years and a median duration of disease of 11.0 years. As expected, most SLE manifestations were more frequent in SLE patients than in iSLE ones and every one of the ACR criteria was also associated with SLE condition; this was particularly true of malar rash, oral ulcers and renal disorder. The analysis-adjusted by gender, age at diagnosis, and disease duration-revealed that higher disease activity, damage and SLE severity index are associated with SLE [OR: 1.14; 95% CI: 1.08-1.20 (P < 0.001); 1.29; 95% CI: 1.15-1.44 (P < 0.001); and 2.10; 95% CI: 1.83-2.42 (P < 0.001), respectively]. These results support the hypothesis that iSLE behaves as a relative stable and mild disease. SLE patients from the RELESSER register do not appear to differ substantially from other Caucasian populations and although activity [median SELENA-SLEDA: 2 (IQ: 0-4)], damage [median SLICC/ACR/DI: 1 (IQ: 0-2)], and severity [median KATZ index: 2 (IQ: 1-3)] scores were low, 1 of every 4 deaths was due to SLE activity. RELESSER represents the largest European SLE registry established to date, providing comprehensive, reliable and updated information on SLE in the southern European population

XII reunión de la Mesa Española de Tratamiento de Aguas Residuales

Desde 1980 el Grupo de Tecnología Ambiental del Departamento de Ingeniería Química y Tecnología del Medio Ambiente de la Universidad de Valladolid trabaja en el desarrollo de tecnologías eficientes, económicas y sostenibles de tratamiento, gestión y valorización de contaminantes, tanto para aguas residuales como para gases y residuos sólidos. La investigación del grupo se ha dirigido principalmente al desarrollo de procesos biológicos, empleando técnicas de biología molecular para su caracterización y seguimiento. Actualmente, el grupo está formado por 10 investigadores senior, 6 post-docs y 17 doctorandos. En los últimos diez años, ha participado en 40 proyectos con financiación pública y 51 con financiación privada, con una producción científica de 27 Tesis Doctorales defendidas, 207 publicaciones JCR, 222 congresos Internacionales y 6 patentes, trabajando en diversas líneas de investigación (http://envtech.uva.es/): - Procesos anaerobios de tratamiento, incluyendo la aplicación de tecnologías de membranas, el estudio de procesos microaeróbicos para la eliminación de H2S, o el enriquecimiento de biogás por conversión biológica de CO2 y H2. - Tratamiento de aguas residuales, con estudios microbiológicos de los procesos de eliminación de nutrientes, análisis y tratamiento de microcontaminantes y combinando eliminación de nutrientes, minimización de fangos y optimización energética. - Tratamiento, minimización y valorización de fangos, aplicando pretratamientos como la explosión de vapor o la hidrólisis térmica para incrementar la producción de biogás. - Tratamiento biológico de aguas residuales mediante consorcios de algas y bacterias, acoplando procesos de oxidación de materia orgánica, eliminación de nutrientes, enriquecimiento de biogás o captura de CO2. - Tratamiento biológico de gases de efecto invernadero, olores y compuestos orgánicos volátiles mediante biorreactores de alta transferencia de materia - Valorización de residuos lignocelulósicos y de biomasa algal para producir bioenergía en forma de alcoholes o de biogás, aprovechando la fracción proteica como biofertilizantes o alimentación animal

Vídeos para apoyo al aprendizaje en las áreas de Ingeniería Química y Tecnologías del Medio Ambiente

Elaboración de screencasts/podcasts (vídeos que resultan de la grabación de imagen de pantalla y el sonido de las explicaciones del docente) para substituir a una parte substancial de las explicaciones teóricas que tradicionalmente se llevan a cabo en el aula. Se han elaborado vídeos de apoyo para la docencia teórica y/o práctica de dos asignaturas del Máster de Ingeniería Química, y bloques de clases teóricas de asignaturas de primer y cuarto curso del Grado Ingeniería Química y se han empleado en la docencia. Se han elaborado también numerosos vídeos con temática relacionada con la docencia del Departamento por los participantes no encuadrados en el desarrollo de medios para las asignaturas citadas.Departamento de Ingeniería Química y Tecnología del Medio AmbienteVídeos elaborados en el PROYECTO DE INNOVACIÓN DOCENTE 2020/2021 Ref:089 "Vídeos para apoyo al aprendizaje en las áreas de Ingeniería Química y Tecnologías del Medio Ambiente

Emergence of Fosfomycin Resistance by Plasmid-Mediated <i>fos</i> Genes in Uropathogenic ESBL-Producing <i>E. coli</i> Isolates in Mexico

Fosfomycin is currently a viable option against urinary tract infections, particularly against extended-spectrum β-lactamases (ESBL)-producing E. coli, due to its unique mechanism of action and its low resistance among bacteria. The objective of this study was to investigate two of the three most common mechanisms of resistance against this antibiotic among 350 ESBL-producing E. coli strains isolated from the urine of Mexican patients. The prevalence of fosfomycin resistance in our study was 10.9% (38/350). Of all resistant isolates analyzed, 23 (60.5%) were identified as fos-producing organisms, with 14 strains carrying fosA3 and 9, fosA1. Additionally, 11 (28.9%) fosfomycin-resistant isolates presented resistance due to impaired antibiotic transport and 8 (21.0%) both mechanisms. No resistance mechanism investigated in the study was found on 12 strains. All 38 confirmed ESBL-producing isolates carried a blaCTX-M subtype, 36 (94.5%) belonged to the O25b-ST131 clone, and all of them were able to transfer the fosfomycin resistance trait to recipient strains horizontally. This is the first study in Mexico demonstrating a plasmid-mediated fosfomycin resistance mechanism among clinical E. coli strains. Since our results suggest a strong association among fos and blaCTX-M genes and ST131 clones in uropathogenic E. coli, plasmid-mediated fosfomycin resistance should be closely monitored

Otro título: Elementos folklóricos y creación estética del Lazarillo de Tormes

Gregorio Marañón Moya, Dir. ICH. Dámaso Alonso, escritor. Elsa Mercado, Embajadora de Panamá en España.Cinta 1: Presentación por parte de Marañón -- Min. 0.24: El secretario general entrega las condecoraciones: Miembros de honor: Yolanda de Arzate Avendeyo. José Solís Ruiz, Mº Scret. Gral. del Movimiento. Antonio Garrigues, Embajador de España en Washington. José Antonio Giménez-Arnau, Embajador de España en Guatemala. James A. Farley, Ex-presidente del partido demócrata de EE.UU. Edmundo Correa, Ex-rector de la Universidad de Cuya. Francisco Javier Sánchez Cantón, Miembro de la Real Academia de la Historia. Federico García Sanchís, Miembro de la RAE. Juán José López Ibor, Catedrático Facultad de Medicina Madrid. Hermenegildo Arruga, Conde de Arruga. José de la Peña, Dir. Archivo de Indias. José Antonio Elola-Olaso, Delegado Nal. de Educación Física y Deportes. Alfonso de la Peña, Catedrático Facultad de Medicina de Madrid. Miembros titulares del ICH: Elvira Pérez Peña, Inst. Cuyano de Cultura Hispánica. Alberico Praga, Rector de la Unv. de Bahía. Francisco Monterde, de la Academia Mexicana de la Lengua. José Barroso, Presidente del Inst. Hispano-Mexicano. Jorge Montoya, Dir. Inst. Antioqueño de Cultura Hispánica. Paul Bouchard, Prof. Hª Precolombina Unv. Laval Canadá. George Age Ornstein, Dir. United Airlines. Luis Alfonso D'Escargnolle, Arquitecto Brasileño de la Casa de Brasil. Juán José Espinosa, Dir. Gral. PSOE. Marcos Peña Royo, Gobernador Civil de Asturias. Plácido Careaga, Pdte. Diputación Vizcaya. José Burgos y Rosado, Marqués de la Liseda. Gabriel Cañadas, Scre. Gral. Mº Infomación y Turismo. Jose María Mor, Dir. Personal Mº Asuntos Exteriores. Fernando Luis Ybarra y López de Dóriga, Marqués de de Agridulce y Pte. Del Ins. Cultura Vascongadas. Antonio Fondán, Dir. Escuela Periodismo Vascongadas. Jose Ignacio Ramos, Delegado de Prensa Embajada de España en Buenos Aires. Salvador Bermúdez, Delegado de Prensa Embajada de España en Lima. Jaime de Abrisqueta, Scre. Embajada de España en Quito. Tomás Lozano, Embajador de España en Honduras. Francisco Javier Chapa, Embajador de España en México. Amaro González de Mesa, Embajador de España en la Santa Sede. Jose Luís Aparicio, Cónsul España en Nueva Orleans. Joaquín Tomás, Cónsul de España en Mendoza. Enrique de la Hoz Díaz, Scret. Gral. Mº de Información -- MIn. 11.35: Conferencia de Dámaso Alonso "Elementos folklóricos y creación estética del Lazarillo de Tormes" -- Cinta 2: Continuación de la conferencia de Dámaso Alonso -- Min. 27.56: Discurso de Elsa Mercado, Embajadora de Panamá en España -- Min. 33.14: Palabras de clausura de Gregorion Marañó

Association Between Use of Enhanced Recovery After Surgery Protocol and Postoperative Complications in Total Hip and Knee Arthroplasty in the Postoperative Outcomes Within Enhanced Recovery After Surgery Protocol in Elective Total Hip and Knee Arthroplasty Study (POWER2)

[Importance] The Enhanced Recovery After Surgery (ERAS) care protocol has been shown to improve outcomes compared with traditional care in certain types of surgery.[Objective] To assess the association of use of the ERAS protocols with complications in patients undergoing elective total hip arthroplasty (THA) and total knee arthroplasty (TKA).[Design, Setting, and Participants] This multicenter, prospective cohort study included patients recruited from 131 centers in Spain from October 22 through December 22, 2018. All consecutive adults scheduled for elective THA or TKA were eligible for inclusion. Patients were stratified between those treated in a self-designated ERAS center (ERAS group) and those treated in a non-ERAS center (non-ERAS group). Data were analyzed from June 15 through September 15, 2019.[Exposures] Total hip or knee arthroplasty and perioperative management. Sixteen individual ERAS items were assessed in all included patients, whether they were treated at a center that was part of an established ERAS protocol or not.[Main Outcomes and Measures] The primary outcome was postoperative complications within 30 days after surgery. Secondary outcomes included length of stay and mortality.[Results] During the 2-month recruitment period, 6146 patients were included (3580 women [58.2%]; median age, 71 [interquartile range (IQR), 63-76] years). Of these, 680 patients (11.1%) presented with postoperative complications. No differences were found in the number of patients with overall postoperative complications between ERAS and non-ERAS groups (163 [10.2%] vs 517 [11.4%]; odds ratio [OR], 0.89; 95% CI, 0.74-1.07; P = .22). Fewer patients in the ERAS group had moderate to severe complications (73 [4.6%] vs 279 [6.1%]; OR, 0.74; 95% CI, 0.56-0.96; P = .02). The median overall adherence rate with the ERAS protocol was 50.0% (IQR, 43.8%-62.5%), with the rate for ERAS facilities being 68.8% (IQR, 56.2%-81.2%) vs 50.0% (IQR, 37.5%-56.2%) at non-ERAS centers (P < .001). Among the patients with the highest and lowest quartiles of adherence to ERAS components, the patients with the highest adherence had fewer overall postoperative complications (144 [10.6%] vs 270 [13.0%]; OR, 0.80; 95% CI, 0.64-0.99; P < .001) and moderate to severe postoperative complications (59 [4.4%] vs 143 [6.9%]; OR, 0.62; 95% CI, 0.45-0.84; P < .001) and shorter median length of hospital stay (4 [IQR, 3-5] vs 5 [IQR, 4-6] days; OR, 0.97; 95% CI, 0.96-0.99; P < .001).[Conclusions and Relevance] An increase in adherence to the ERAS program was associated with a decrease in postoperative complications, although only a few ERAS items were individually associated with improved outcomes.This study was supported by institutional and/or departmental sources. The Postoperative Outcomes Within Enhanced Recovery After Surgery Protocol in Elective Total Hip and Knee Arthroplasty (POWER2) study was supported by the Spanish Perioperative Audit and Research Network (REDGERM