Meio século de artes plásticas em Cuba

Durante las recientes décadas, las artes plásticas en Cuba han desarrollado diversas tendencias como la abstracción, el fotorrealismo, el neoexpresionismo, el conceptualismo, etc., mientras mantienen algunas temáticas y caracteres cuya base se remite a las culturas afrocubanas. A las manifestaciones con antecedentes nacionales se añadirá, como manifestación orgánica, el diseño gráfico que se ha constituido en una escuela de caracteres propios. En la actualidad proliferan las instalaciones y los performances, a menudo con carácter interdisciplinario, así como el video art.Durante as recentes décadas, as artes plásticas em Cuba desenvolveram diversas tendências como a abstração, o fotorrealismo, o neoexpressionismo, o conceitualismo etc., enquanto algumas temáticas e alguns caracteres cuja base remete às culturas afro-cubanas são mantidos. Às manifestações com antecedentes nacionais se agregará, como manifestação orgânica, o design gráfico, que vem se constituindo numa escola de particularidades próprias. Na atualidade proliferam as instalações e as performances, muitas vezes com caráter interdisciplinar, como no caso da video art.During recent decades, several trends have emerged in the Cuban visual arts, e.g., abstraction, photorealism, neoexpressionism and conceptualism, among others, without losing sight of certain themes and characters based on Afro-Cuban cultures. To the manifestations of a national heritage should be added, as an organic expression, graphic design, which is becoming a school with unique features. Today, we have a proliferation of performances and installations, often of interdisciplinary nature, as is the case of video art

The AHAWOMEN project: study protocol of a multi‑design research for exploring HAPA predictors of exercise in postmenopausal women

This research is funded by FEDER/Junta de Andalucía — Consejería de Transformación Económica, Industria, Conocimiento y Universidades (I + D + I Programa Operativo FEDER de Andalucía 2014–2020). Research Project B-CTS-342-UGR20 (2021–23). This research project has obtained formal ethical review by the Ethics Committee on Human Research of the University of Granada (Spain), reg. B-CTS-342-UGR20 (17/11/2021) and has undergone independent peer-review from the funding body (29/07/2021). The funding body has no role in the design of the study, the collection, analysis and interpretation of data and the writing of the manuscript.Background The postmenopausal period can represent an opportunity for women to improve their health and well-being. The Active and Healthy Ageing in Women during early postmenopause (AHAWOMEN) study aims to identify the key determinants of an active lifestyle among middle-aged women, with a focus on the stages and the social-cognitive variables outlined in the Health Action Process Approach (HAPA) model, a theoretical framework for understanding health behaviour change. We expected that HAPA factors and processes of intention creation (motivational phase) and action adoption (volitional phase) will be significant predictors of exercise initiation and maintenance, supporting both the HAPA tenets and the efficacy of HAPA-based interventions. Methods/design This study was approved by the authors’ Institutional Review Committee. Postmenopausal women aged between 45 and 65 years will voluntarily participate. The participants will be allocated to one of three groups: Intervention-Initiators (n = 100, random allocation), Control-Sedentary (n = 100, random allocation) or Control-Active (n = 100, non-random allocation). The intervention group will engage in a supervised exercise programme lasting at least 3 months, supplemented with a HAPA-based intervention for behaviour change. The sedentary control group will not receive any intervention to change their physical activity, while the active control group will consist of women who are already regularly adhering to an active lifestyle. Study variables will be measured at baseline and postintervention phases, as well as at 1, 3, 6 and 12-month follow-ups. The predictors of exercise behaviour in the different phases of the behavioural change process will be explored and compared within and between groups throughout the study. These analyses will help identify the factors that determine the adoption of a healthy active behaviour. Additionally, the effectiveness of the model and the intervention for changing active behaviour will be evaluated. Discussion This paper describes the rationale, development and methods used in the AHAWOMEN project. Supporting women who intend to become active can help them to translate their goals into sustainable action. Verifying that the HAPA predictions are applicable to postmenopausal women’s adoption of exercise would provide the basis for designing effective interventions for promoting healthy and active ageing that are also tailored to the experiences of middle-aged women.FEDER/Junta de Andalucía B-CTS-342-UGR20University of Granada B-CTS-342-UGR2

Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury

[EN]Using an immunohistochemical technique, we have studied the distribution of kynuneric acid (KYNA) and 3-hydroxyanthranilic acid (3-HAA) in a rat brain injury model (trauma). The study was carried out inducing a cerebral ablation of the frontal motor cortex. Two mouse monoclonal specific antibodies previously developed by our group directed against KYNA and 3-HAA were used. In control animals (sham-operated), the expression of both KYNA and 3-HAA was not observed. In animals in which the ablation was performed, the highest number of immunoreactive cells containing KYNA or 3-HAA was observed in the region surrounding the lesion and the number of these cells decreased moving away from the lesion. KYNA and 3-HAA were also observed in the white matter (ipsilateral side) located close to the injured region and in some cells placed in the white matter of the contralateral side. The distribution of KYNA and 3-HAA perfectly matched with the peripheral injured regions. The results found were identical independently of the perfusion date of animals (17, 30 or 54 days after brain injury). For the first time, the presence of KYNA and 3-HAA has been described in a rat trauma model

Trasplantes amigdalares embrionarios en ratas adultas con lesiones de la corteza motora: análisis molecular y electrofisiológico

Transplants of embryonic nervous tissue ameliorate motor deficits induced by motor cortex lesions in adult animals. Restoration of lost brain functions has been recently shown in grafts of homotopic cortical origin, to be associated with a functional integration of the transplant after development of reciprocal host-graft connections. Nevertheless little is known about physiological properties or gene expression profiles of cortical implants with functional restorative capacity but no cortical origin. In this study, we show molecular and electrophysiological evidence supporting the functional development and integration of heterotopic transplants of embryonic amygdalar tissue placed into pre-lesioned motor cortex of adult rats. Grafts were analyzed 3 months post-transplantation

Characterisation of youth entrepreneurship in Medellín-Colombia using machine learning

The aim of this paper is to identify profiles of young Colombian entrepreneurs based on data from the “Youth Entrepreneurship” survey developed by the Colombian Youth Secretariat. Our research results show five profiles of entrepreneurs, mainly differentiated by age and entrepreneurial motives, as well as the identification of relevant skills, capacities, and capabilities for entrepreneurship, such as creativity, learning, and leadership. The sample consists of 633 young people aged between 14 and 28 years in Medellín. The data treatment was approached through cluster analysis using the K-means algorithm to obtain information about the underlying nature and structure of the data. These data analysis techniques provide valuable information that can help to better understand the behaviour of Colombian entrepreneurs. They also reveal hidden information in the data. Therefore, one of the advantages of using statistical and artificial intelligence techniques in this type of study is to extract valuable information that might otherwise go unnoticed. The clusters generated show correlations with profiles that can support the design of policies in Colombia to promote an entrepreneurial ecosystem and the creation and development of new businesses through business regulation

Diplomado de profundización cisco (diseño e implementación de soluciones integradas LAN / WAN)

Cada día en nuestra sociedad estamos utilizando los medios de comunicación en especial la internet que integra todas las formas de comunicaciones existentes, por ello se hace indispensable que sepamos como es el funcionamiento desde adentro, para así comprender más fácilmente su operación, en este trabajo se ha abordado una serie de temáticas que tienen que ver con el funcionamiento de las redes de datos, por tal motivo aprenderemos a configurar, un router para que opere el direccionamiento OSPF, y así configurar muchos parámetros que hacen parte del correcto trabajo de las redes de datos, también encontraremos como crear direccionamiento DHCP, para que los enrutadores asignen, las direcciones de red automáticamente, y que los demás clientes, puedan obtener de forma automática su dirección IP, también trataremos la traducción de direcciones de ip para IPv4, y demás temáticas que son de gran ayuda a los profesionales que seremos en un futuro cercano, todo inclinado hacia la solución de los diferentes problemas que se podrían presentar en un red de comunicación, que integre elementos que se están viendo en este cursoEvery day in our society we are using the media in especially the internet that integrates all forms of existing communications, so It makes it indispensable that we know how is the operation from inside, so more easily understand their operation, in this work a series of topics that have to do with the functioning of data networks, for this reason we will learn to configure, a router to operate the OSPF address, and configure many parameters that are part of the correct work of data networks, we will also find how to create DHCP addressing, so that the routers assign, the network addresses automatically, and that other customers can obtain automatically your IP address, we will also deal with the translation of ip addresses for IPv4, and other topics that are of great help to professionals that we will be in a near future, all inclined towards the solution of the different problems that could be present in a communication network, that integrates elements that are being seen in this cours

Correlation of RECIST, Computed Tomography Morphological Response, and Pathological Regression in Hepatic Metastasis Secondary to Colorectal Cancer: The AVAMET Study.

Background: The prospective phase IV AVAMET study was undertaken to correlate response evaluation criteria in solid tumors (RECIST)-defined response rates with computed tomography-based morphological criteria (CTMC) and pathological response after liver resection of colorectal cancer metastases. Methods: Eligible patients were aged >/=18 years, with Eastern Cooperative Oncology Group (ECOG) performance status 0/1 and histologically-confirmed colon or rectal adenocarcinoma with measurable liver metastases. Preoperative treatment was bevacizumab (7.5 mg on day 1) + XELOX (oxaliplatin 130 mg/m(2), capecitabine 1000 mg/m(2) bid on days 1-14 q3w). After three cycles, response was evaluated by a multidisciplinary team. Patients who were progression-free and metastasectomy candidates received one cycle of XELOX before undergoing surgery 3-5 weeks later, followed by four cycles of bevacizumab + XELOX. Results: A total of 83 patients entered the study; 68 were eligible for RECIST, 67 for CTMC, and 51 for pathological response evaluation. Of these patients, 49% had a complete or partial RECIST response, 91% had an optimal or incomplete CTMC response, and 81% had a complete or major pathological response. CTMC response predicted 37 of 41 pathological responses versus 23 of 41 responses predicted using RECIST (p = 0.008). Kappa coefficients indicated a lack of correlation between the results of RECIST and morphological responses and between morphological and pathological response rates. Conclusion: CTMC may represent a better marker of pathological response to bevacizumab + XELOX than RECIST in patients with potentially-resectable CRC liver metastases

Comparative effects of A- and B-type proanthocyanidins in the prevention of urinary tract infection in mice

Resumen del póster presentado a la VI International Conference on Polyphenols and Health celebrada en Buenos Aires (Argentina) del 16 al 19 de octubre de 2013.Consumption of cranberry (Vaccinium macrocarpum) is widely recommended forprophylaxis against urinary tract infections (UTI) in women. Among cranberry components, A-type proanthocyanins would be implicated in these preventive effects against UTI. However, proanthocyanidins are poorly absorbed in the small intestine, but subjected to extensive biotransformation in the colon, although studies are almost restricted to B-type proanthocyanidins. Therefore, the hypothesis of this study is that urinary metabolome from of A-type and B-type proanthocyanidins-mainly derived from their colonic catabolism-differ,and only metabolites from the A-type procyanidins have protective effects against UTI. To test this hypothesis, JAXc3H/OuJ female mice previously fed with specific diet (control, 1% cranberry extract and 1% grape seed extract) for 2 weeks, were inoculated with the uropathogenic E. coli (ATCC 53503™) to provoke infection, and maintained 2 weeks more before being sacrificed. Urine samples were collected at different times and subjected to E.coli counting, leukocytary esterase and nitrites analyses, and mieloperoxidase task. Samples of kidney and bladder tissues were also collected for E. coli counting and histopathologic analysis. Additionally, the capacity of the urine samples to inhibit bacterial adherence was tested in the T24 bladder cell line (ATCC HTB4 ™).Peer reviewe

Tumor cell and immune cell profiles in primary human glioblastoma: Impact on patient outcome

© 2020 The Authors.The distribution and role of tumor-infiltrating leucocytes in glioblastoma (GBM) remain largely unknown. Here, we investigated the cellular composition of 55 primary (adult) GBM samples by flow cytometry and correlated the tumor immune profile with patient features at diagnosis and outcome. GBM single-cell suspensions were stained at diagnosis (n = 44) and recurrence following radiotherapy and chemotherapy (n = 11) with a panel of 8-color monoclonal antibody combinations for the identification and enumeration of (GFAPCD45) tumor and normal astrocytic cells, infiltrating myeloid cells —i.e. microglial and blood-derived tumor-associated macrophages (TAM), M1-like, and M2-like TAM, neutrophils. and myeloid-derived suppressor cells (MDSC)— and tumor-infiltrating lymphocytes (TIL) —i.e. CD3T-cells and their TCD4, TCD8, TCD4CD8, and (CD25CD127) regulatory (T-regs) subsets, (CD19CD20) B-cells, and (CD16) NK-cells—. Overall, GBM samples consisted of a major population (mean ± 1SD) of tumor and normal astrocytic cells (73% ± 16%) together with a significant but variable fraction of immune cells (24% ± 18%). Within myeloid cells, TAM predominated (13% ± 12%) including both microglial cells (10% ± 11%) and blood-derived macrophages (3% ± 5%), in addition to a smaller proportion of neutrophils (5% ± 9%) and MDSC (4% ± 8%). Lymphocytes were less represented and mostly included TCD4 (0.5% ± 0.7%) and TCD8 cells (0.6% ± 0.7%), together with lower numbers of TCD4CD8 T-cells (0.2% ± 0.4%), T-regs (0.1% ± 0.2%), B-lymphocytes (0.1% ± 0.2%) and NK-cells (0.05% ± 0.05%). Overall, three distinct immune profiles were identified: cases with a minor fraction of leucocytes, tumors with a predominance of TAM and neutrophils, and cases with mixed infiltration by TAM, neutrophils, and T-lymphocytes. Untreated GBM patients with mixed myeloid and lymphoid immune infiltrates showed a significantly shorter patient overall survival versus the other two groups, in the absence of gains of the EGFR gene (p = 0.02). Here we show that immune cell infiltrates are systematically present in GBM, with highly variable levels and immune profiles. Patients with mixed myeloid and T-lymphoid infiltrates showed a worse outcome.Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain and fondos FEDER, Grant/Award Number: CB16/12/00400 and ISCIII PI16/0476; Consejería de Sanidad Junta de Castilla y León, Gerencia Regional de Salud, Spain, Grant/Award Number: GRS2049/A/1

Statistical Evaluation of Metaproteomics and 16S rRNA Amplicon Sequencing Techniques for Study of Gut Microbiota Establishment in Infants with Cystic Fibrosis

Newborn screening for cystic fibrosis (CF) can identify affected but asymptomatic infants. The selection of omic technique for gut microbiota study is crucial due to both the small amount of feces available and the low microorganism load. Our aims were to compare the agreement between 16S rRNA amplicon sequencing and metaproteomics by a robust statistical analysis, including both presence and abundance of taxa, to describe the sequential establishment of the gut microbiota during the first year of life in a small size sample (8 infants and 28 fecal samples). The taxonomic assignations by the two techniques were similar, whereas certain discrepancies were observed in the abundance detection, mostly the lower predicted relative abundance of Bifidobacterium and the higher predicted relative abundance of certain Firmicutes and Proteobacteria by amplicon sequencing. During the first months of life, the CF gut microbiota is characterized by a significant enrichment of Ruminococcus gnavus, the expression of certain virulent bacterial traits, and the detection of human inflammation-related proteins. Metaproteomics provides information on composition and functionality, as well as data on host-microbiome interactions. Its strength is the identification and quantification of Actinobacteria and certain classes of Firmicutes, but alpha diversity indices are not comparable to those of amplicon sequencing. Both techniques detected an aberrant microbiota in our small cohort of infants with CF during their first year of life, dominated by the enrichment of R. gnavus within a human inflammatory environment. IMPORTANCE In recent years, some techniques have been incorporated for the study of microbial ecosystems, being 16S rRNA gene sequencing being the most widely used. Metaproteomics provides the advantage of identifying the interaction between microorganisms and human cells, but the available databases are less extensive as well as imprecise. Few studies compare the statistical differences between the two techniques to define the composition of an ecosystem. Our work shows that the two methods are comparable in terms of microorganism identification but provide different results in alpha diversity analysis. On the other hand, we have studied newborns with cystic fibrosis, for whom we have described the establishment of an intestinal ecosystem marked by the inflammatory response of the host and the enrichment of Ruminococcus gnavus