Adipogenesis in Rodent Skin, The Dermal Adipose Layer: What Signals Adipocyte Development in the Lower Dermis?

Research into adipogenesis has become increasingly more fundamental as our understanding of diabetes and obesity develops. Adipose tissue (fat) exists in many depots throughout the body. While widely known that there is a subcutaneous adipose depot beneath the skin, more recently a separate dermal adipose layer, within the lower dermis of rodent skin, has been found. However, the developmental origins and timings of adipogenesis in this dermal layer are not well characterised. This thesis aims to study adipogenesis within this layer, by creating an in vivo-like model and investigating signalling pathway effects. Firstly, the hypothesis of an inhibition signal from the upper dermis and the role of the G0/G1 switch gene 2 (G0S2) in the dermal adipose tissue was explored. Experiments showed G0S2 expression in the lower dermis during development. PPARγ has been shown as a main regulator of adipogenesis, together with C/EBPα. The potential role of PPARγ in early adipogenesis was investigated, using immunofluorescence analysis. Microarray analysis studies previously showed molecular differences between in vitro and in vivo, therefore model systems, more closely related to in vivo conditions, are necessary. An organ culture and a cell culture model were developed. The cell culture model, using 3D cell spheres, proved most useful to investigate signalling in adipocyte development. The EGF/EGFR, TGFβ/BMP and KGF/KGFR pathways were studied, by supplementing cultures with an activator or inhibitor of the receptor of each pathway. Differential levels of oil red O staining and therefore adipocyte formation, were observed. In conclusion, this work was found to suggest commitment of the cells to the adipocyte lineage begins earlier, than from when microarray data was collected. While not conclusive, it provides an insight and direction for further studies, in terms of PPARγ and potential adipogenic inhibition signals in the upper dermis, as well as a model system

NASA Provides the Capability to Deliver Near Real-Time JPSS Data to Users in Order to Monitor Time-Sensitive Applications Such as Wildfires, Floods, Volcanic Eruptions, Tropical Cyclones and Extreme Weather Events

NASA's Land, Atmosphere Near real-time Capability for EOS (Earth Observing System) (LANCE https://earthdata.nasa.gov/lance) serves near real time (NRT) data to monitor time sensitive applications such as monitoring wildfires, floods, volcanic eruptions, tropical cyclones and extreme weather events. It currently serves data and imagery from the Visible Infrared Imager Radiometer Suite (VIIRS) and Ozone Mapping and Profiler Suite (OMPS) S NPP (Suomi National Polar-orbiting Partnership) instruments and is in the process of integrating continuity data products from VIIRS and OMPS onboard the Joint Polar Satellite System (JPSS), via the JPSS data Hub, to continue to meet the needs of agencies, scientists and members of the general public. NASA's Earth Science Division (ESD) sponsored the EOSDIS development of LANCE in 2009 to provide a central point of access to high quality NRT data products and imagery for applications users. LANCE makes data available to the public within 3 hours of satellite observation and imagery within 4-5 hours of satellite observation. Full resolution browse imagery from LANCE are provided through the Global Imagery Browse Services (GIBS) which also fuels NASA's Worldview tool so that users can interactively browse near real time data. This data supports time critical applications and allows users to view current natural hazards and events and animate the imagery over time

Effects of audibility and multichannel wide dynamic range compression on consonant recognition for listeners with severe hearing loss

Objective—This study examined the effects of multichannel wide-dynamic range compression (WDRC) amplification and stimulus audibility on consonant recognition and error patterns. Design—Listeners had either severe or mild-to-moderate sensorineural hearing loss. Each listener was monaurally fit with a wearable hearing aid using typical clinical procedures, frequency-gain parameters and a hybrid of clinically prescribed compression ratios for DSL (Scollie et al., 2005) and NAL-NL (Dillon, 1999). Consonant-vowel nonsense syllables were presented in soundfield at multiple input levels (50, 65, 80 dB SPL). Test conditions were four-channel fast-acting WDRC amplification and a control compression limiting (CL) amplification condition. Listeners identified the stimulus heard from choices presented on an on-screen display. A between-subject repeated measures design was used to evaluate consonant recognition and consonant confusion patterns. Results—Fast-acting WDRC provided a considerable audibility advantage at 50 dB SPL, especially for listeners with severe hearing loss. Listeners with mild-to-moderate hearing loss received less audibility improvement from the fast-acting WDRC amplification, for conversational and high level speech, compared to listeners with severe hearing loss. Analysis of WDRC benefit scores revealed that listeners had slightly lower scores with fast-acting WDRC amplification (relative to CL) when WDRC provided minimal improvement in audibility. The negative effect was greater for listeners with mild-to-moderate hearing loss compared to their counterparts with severe hearing loss. Conclusions—All listeners, but particularly the severe loss group, benefited from fast-acting WDRC amplification for low-level speech. For conversational and higher speech levels (i.e., when WDRC does not confer a significant audibility advantage), fast-acting WDRC amplification appears to slightly degrade performance. Listeners’ consonant confusion patterns suggest that this negative effect may be partly due to fast-acting WDRC-induced distortions which alter specific consonant features. In support of this view, audibility accounted for a greater percentage of the variance in listeners’ performance with CL amplification compared to fast-acting WDRC amplification

Estimating morbidity due to stroke in Nigeria: a systematic review and meta-analysis

The response to stroke in Nigeria is impaired by inadequate epidemiologic information. We sought to collate available evidence and estimate the incidence of stroke and prevalence of stroke survivors in Nigeria. Using random effects meta-analysis, we pooled nationwide and regional incidence and prevalence of stroke from the estimates reported in each study. Eleven studies met our selection criteria. The pooled crude incidence of stroke in Nigeria was 26.0 (12.8-39.0) /100,000 person-years, with this higher among men at 34.1 (9.7-58.4) /100,000, compared to women at 21.2 (7.4-35.0) /100,000. The pooled crude prevalence of stroke survivors in Nigeria was 6.7 (5.8-7.7) /1000 population, with this also higher among men at 6.4 (5.1-7.6) /1000, compared to women at 4.4 (3.4-5.5) /1000. In the period 2000-2009, the incidence of stroke in Nigeria was 24.3 (95% CI: 11.9-36.8) per 100,000, with this increasing to 27.4 (95% CI: 2.2-52.7) per 100,000 from 2010 upwards. The prevalence of stroke survivors increased minimally from 6.0 (95% CI: 4.6-7.5) per 1000 to 7.5 (95% CI: 5.8-9.1) per 1000 over the same period. The prevalence of stroke survivors was highest in the South-south region at 13.4 (9.1-17.8) /100,000 and among rural dwellers at 10.8 (7.5-14.1) /100,000. Although study period does not appear to contribute substantially to variations in stroke morbidity in Nigeria, an increasing number of new cases compared to survivors may be due in part to limited door-door surveys, or possibly reflects an increasing mortality from stroke in the country. [Abstract copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Witchcraft and the Somerset idyll : The depiction of folk belief in Walter Raymond’s novels

The work of Walter Raymond (1852-1931) is now largely forgotten. Yet his Somerset novels, complemented by his ethnographic writings, contain depictions of local witchcraft belief that are worthy of study in literary and historical contexts. They raise issues regarding the fictional depiction of rural life and tradition, and the value of fiction as a folkloric and historical sourcePeer reviewe

Protocol for a mixed-methods study to develop and feasibility test a digital system for the capture of Patient-Reported Outcomes (PROs) in patients receiving Chimeric Antigen Receptor T-cell (CAR-T) therapies (The PRO-CAR-T Study)

Introduction: Chimeric antigen receptor (CAR) T-cell therapies are novel, potentially curative therapies for haematological malignancies. CAR T-cell therapies are associated with severe toxicities, meaning patients require monitoring during acute and postacute treatment phases. Electronic patient-reported outcomes (ePROs), self-reports of health status provided via online questionnaires, can complement clinician observation with potential to improve patient outcomes. This study will develop and evaluate feasibility of a new ePRO system for CAR-T patients in routine care. Methods and analysis: Multiphase, mixed-methods study involving multiple stakeholder groups (patients, family members, carers, clinicians, academics/researchers and policy-makers). The intervention development phase comprises a Delphi study to select PRO measures for the digital system, a codesign workshop and consensus meetings to establish thresholds for notifications to the clinical team if a patient reports severe symptoms or side effects. Usability testing will evaluate how users interact with the digital system and, lastly, we will evaluate ePRO system feasibility with 30 CAR-T patients (adults aged 18+ years) when used in addition to usual care. Feasibility study participants will use the ePRO system to submit self-reports of symptoms, treatment tolerability and quality of life at specific time points. The CAR-T clinical team will respond to system notifications triggered by patients’ submitted responses with actions in line with standard clinical practice. Feasibility measures will be collected at prespecified time points following CAR T-cell infusion. A qualitative substudy involving patients and clinical team members will explore acceptability of the ePRO system. Ethics and dissemination: Favourable ethical opinion was granted by the Health and Social Care Research Ethics Committee B(HSC REC B) (ref: 23/NI/0104) on 28 September 2023. Findings will be submitted for publication in high-quality, peer-reviewed journals. Summaries of results, codeveloped with the Blood and Transplant Research Unit Patient and Public Involvement and Engagement group, will be disseminated to all interested groups. Trial registration number: ISCTRN11232653

Feminist Reflections on the Scope of Labour Law: Domestic Work, Social Reproduction and Jurisdiction

Drawing on feminist labour law and political economy literature, I argue that it is crucial to interrogate the personal and territorial scope of labour. After discussing the “commodification” of care, global care chains, and body work, I claim that the territorial scope of labour law must be expanded beyond that nation state to include transnational processes. I use the idea of social reproduction both to illustrate and to examine some of the recurring regulatory dilemmas that plague labour markets. I argue that unpaid care and domestic work performed in the household, typically by women, troubles the personal scope of labour law. I use the example of this specific type of personal service relation to illustrate my claim that the jurisdiction of labour law is historical and contingent, rather than conceptual and universal. I conclude by identifying some of the implications of redrawing the territorial and personal scope of labour law in light of feminist understandings of social reproduction