Detailed Spectral Modeling of a 3-D Pulsating Reverse Detonation Model: Too Much Nickel

We calculate detailed NLTE synthetic spectra of a Pulsating Reverse Detonation (PRD) model, a novel explosion mechanism for Type Ia supernovae. While the hydro models are calculated in 3-D, the spectra use an angle averaged hydro model and thus some of the 3-D details are lost, but the overall average should be a good representation of the average observed spectra. We study the model at 3 epochs: maximum light, seven days prior to maximum light, and 5 days after maximum light. At maximum the defining Si II feature is prominent, but there is also a prominent C II feature, not usually observed in normal SNe Ia near maximum. We compare to the early spectrum of SN 2006D which did show a prominent C II feature, but the fit to the observations is not compelling. Finally we compare to the post-maximum UV+optical spectrum of SN 1992A. With the broad spectral coverage it is clear that the iron-peak elements on the outside of the model push too much flux to the red and thus the particular PRD realizations studied would be intrinsically far redder than observed SNe Ia. We briefly discuss variations that could improve future PRD models.Comment: 15 pages, 4 figures, submitted to Ap

Effect of hypoxia on lung gene expression and proteomic profile: insights into the pulmonary surfactant response

Exposure of lung to hypoxia has been previously reported to be associated with significant alterations in the protein content of bronchoalveolar lavage (BAL) and lung tissue. In the present work we have used a proteomic approach to describe the changes in protein complement induced by moderate long-term hypoxia (rats exposed to 10% O2 for 72h) in BAL and lung tissue, with a special focus on the proteins associated with pulmonary surfactant, which could indicate adaptation of this system to limited oxygen availability. The analysis of the general proteomic profile indicates a hypoxia-induced increase in proteins associated with inflammation both in lavage and lung tissue. Analysis at mRNA and protein levels revealed no significant changes induced by hypoxia on the content in surfactant proteins or their apparent oligomeric state. In contrast, we detected a hypoxia-induced significant increase in the expression and accumulation of hemoglobin in lung tissue, at both mRNA and protein levels, as well as an accumulation of hemoglobin both in BAL and associated with surface-active membranes of the pulmonary surfactant complex. Evaluation of pulmonary surfactant surface activity from hypoxic rats showed no alterations in its spreading ability, ruling out inhibition by increased levels of serum or inflammatory proteins.Ministerio de Ciencia BIO2012-30733Ministerio de Ciencia CSD2007-00010Gobierno de la Comunidad de Madrid S2009MAT-1507National Institutes of Health NIH HL3478

Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling in a microcosm soil experiments

The oxalate-carbonate pathway (OCP) leads to a potential carbon sink in terrestrial environments. This process is linked to the activity of oxalotrophic bacteria. Although isolation and molecular characterizations are used to study oxalotrophic bacteria, these approaches do not give information on the active oxalotrophs present in soil undergoing the OCP. The aim of this study was to assess the diversity of active oxalotrophic bacteria in soil microcosms using the Bromodeoxyuridine (BrdU) DNA labeling technique. Soil was collected near an oxalogenic tree (Milicia excelsa). Different concentrations of calcium oxalate (0.5%, 1%, and 4% w/w) were added to the soil microcosms and compared with an untreated control. After 12 days of incubation, a maximal pH of 7.7 was measured for microcosms with oxalate (initial pH 6.4). At this time point, a DGGE profile of the frc gene was performed from BrdU-labeled soil DNA and unlabeled soil DNA. Actinobacteria (Streptomyces- and Kribbella-like sequences), Gammaproteobacteria and Betaproteobacteria were found as the main active oxalotrophic bacterial groups. This study highlights the relevance of Actinobacteria as members of the active bacterial community and the identification of novel uncultured oxalotrophic groups (i.e. Kribbella) active in soil

Leptin and Hypertension in Obesity

Leptin, a peptide discovered more than 10 years ago, decreases food intake and increases sympathetic nerve activity to both thermogenic and nonthermogenic tissue. Leptin was initially believed to be an anti-obesity hormone, owing to its metabolic effects. However, obese individuals, for unknown reasons, become resistant to the satiety and weight-reducing effect of the hormone, but preserve leptin-mediated sympathetic activation to nonthermogenic tissue such as kidney, heart, and adrenal glands. Leptin has been shown to influence nitric oxide production and natriuresis, and along with chronic sympathetic activation, especially to the kidney, it may lead to sodium retention, systemic vasoconstriction, and blood pressure elevation. Consequently, leptin is currently considered to play an important role in the development of hypertension in obesity

Anderson Photon-Phonon Colocalization in Certain Random Superlattices

Fundamental observations in physics ranging from gravitational wave detection to laser cooling of a nanomechanical oscillator into its quantum ground state rely on the interaction between the optical and the mechanical degrees of freedom. A key parameter to engineer this interaction is the spatial overlap between the two fields, optimized in carefully designed resonators on a case-by-case basis. Disorder is an alternative strategy to confine light and sound at the nanoscale. However, it lacks an a priori mechanism guaranteeing a high degree of colocalization due to the inherently complex nature of the underlying interference processes. Here, we propose a way to address this challenge by using GaAs/AlAs vertical distributed Bragg reflectors with embedded geometrical disorder. Because of a remarkable coincidence in the physical parameters governing light and motion propagation in these two materials, the equations for both longitudinal acoustic waves and normal-incidence light become practically equivalent for excitations of the same wavelength. This guarantees spatial overlap between the electromagnetic and displacement fields of specific photon-phonon pairs, leading to strong light-matter interaction. In particular, a statistical enhancement in the vacuum optomechanical coupling rate, go, is found, making this system a promising candidate to explore Anderson localization of high frequency (∼20 GHz) phonons enabled by cavity optomechanics. The colocalization effect shown here unlocks the access to unexplored localization phenomena and the engineering of light-matter interactions mediated by Anderson-localized states

Gravitational Settling of 22Ne in Liquid White Dwarf Interiors

The nuclear reactions that occur in the stellar progenitors of white dwarfs (WDs) lead to an internal composition of 12C, 16O, and a ``contaminant'' nucleus, 22Ne. The 22Ne is produced by helium captures on 14N left from hydrogen burning via the CNO cycle. By virtue of its two excess neutrons (relative to the predominant A=2Z nuclei), a downward force of 2m_pg is exerted on 22Ne in the WD interior. This biases its diffusive equilibrium, forcing 22Ne to settle towards the center of the WD. We discuss the physics of the gravitational settling when the WD is in the liquid state and the luminosity generated by it. This modifies the cooling of WD's with masses in excess of a solar mass. The current uncertainties in the microphysics even allow for solutions where a 1.2M_\odot WD remains mostly liquid for a few Gyrs due to the internal heating from 22Ne sedimentation. This highlights the need for an accurate calculation of the inter-diffusion coefficient, especially in the quantum liquid regime relevant for high mass WD's. There is also time in old, liquid WD's (such as those found in cataclysmic variables and possibly in accreting Type Ia progenitors) for partial settling.Comment: To Appear in Ap. J. Letters, 6 pages, 2 figure

Changes in whole body bone mineral composition in a community-based pilot study designed for Mexican-American women at risk for type II diabetes

Changes in whole body bone mineral composition in a community-based pilot study designed for Mexican-American women at risk for type II diabetes David C. Castillo, B.S., Maria G. Placeres, B.S., Arely Perez, M.S., Danielle M. Bravo, B.S., Donovan L. Fogt, Ph.D., Zenong Yin, Ph.D. Mobile Health Laboratory, Department of Health and Kinesiology The University of Texas at San Antonio Classification of First Author (Master’s) Background: Osteoporosis is a serious public health concern in the United States that is expected to increase over the next several years, especially in women. The US Surgeon General’s Report on Bone Health states that individuals can decrease the risk of developing osteoporosis with proper combinations of nutrition and physical activity. Diet and exercise are two important factors that have been shown to prevent or delay the onset of type II diabetes, another national top health concern. Intensive lifestyle interventions, such as The Diabetes Prevention Program have shown that type II diabetes can be delayed or prevented by losing moderate amounts of weight through dietary changes and increased physical activity. It is not clear if a lifestyle intervention can impact bone health. Purpose: This pilot study examined the effects of lifestyle intervention (dietary and physical activity behavior modification) on bone mineral density (BMD) and bone mineral content (BMC). The intervention was a 14-week community-based pilot study, based on The Diabetes Prevention Program, designed to reduce risk for type II diabetes in high-risk Mexican American women. The research questions were: Did the lifestyle intervention affect the outcome measures (BMC and BMD)? Were there differences in BMC and BMD between age groups (low-age ≤45 yrs. vs. high-age \u3e45 yrs.)? Were there differences in BMC and BMD between body weight groups (≤78 kg vs. \u3e78.1 kg)? Methods: The study used a one-group pre- and post-test design. Twenty-five non-diabetic Mexican-American females (average age = 45, SD = 10.9; BMI 25-40) participated in a 14-week lifestyle intervention pilot study. Changes in BMD (g/cm2) and BMC (g) were measured at baseline and 14 weeks after baseline using whole body dual-energy x-ray absorptiometry (DXA). Results: results of paired t-test showed a significant increase in whole body BMC (p\u3c .0001) and a marginally significant increase in whole body BMD (p\u3c .06). Results of Analysis of Covariance revealed no significant difference in BMC between age groups and a marginally significant increase of BMD (p\u3c .07) in younger participants compared to older participants, after controlling pretest measure. There was a significant increase in BMC (p\u3c .01) and a marginally significant increase in BMD (p\u3c .08) in the high-body weight group compared to the low weight group after controlling for pretest measure. Conclusions: A lifestyle intervention that utilized a combination of physical activity and dietary modification showed great promise toward preventing the onset of osteoporosis, especially in heavier Mexican-American women

Supporting the inclusion and retention of autistic students: Exploring teachers' and paraeducators' use of evidence-based practices in public elementary schools

Introduction: Educators in public schools are required to serve students in their least restrictive environment. While many evidence-based practices (EBPs), defined as practices and strategies shown by research to have meaningful effectson outcomes for autistic students are documented in the literature, less is known about EBP use among educators in public schools. Methods: Eighty-six general and special education teachers and para educators completed a survey about familiarity, training, and EBP use for included autistic children. Results: Across roles, educators reported familiarity (98.8%), use (97.7%), and training (83.7%) in reinforcement. They reported the least familiarity with behavioral momentum (29.1%), training in both video modeling and peer-mediated instruction and intervention (18.6%), and use of video modeling (14.0%). Follow-up interviews (n = 80) highlighted mixed understanding of EBP definitions and use. Discussion: Implications for inclusive education are discussed including autism-specific EBP training within pre-service teacher preparation programs

Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis

The Clostridium-related poultry disease, necrotic enteritis (NE), causes substantial economic losses on a global scale. In the present study, a mixture of two plant-derived phytonutrients, Capsicum oleoresin and turmeric oleoresin (XT), was evaluated for its effects on local and systemic immune responses using a co-infection model of experimental NE in commercial broilers. Chickens were fed from hatch with a diet supplemented with XT, or with a non-supplemented control diet, and either uninfected or orally challenged with virulent Eimeria maxima oocysts at 14d and Clostridium perfringens at 18d of age. Parameters of protective immunity were as follows: (1) body weight; (2) gut lesions; (3) serum levels of C. perfringens α-toxin and NE B-like (NetB) toxin; (4) serum levels of antibodies to α-toxin and NetB toxin; (5) levels of gene transcripts encoding pro-inflammatory cytokines and chemokines in the intestine and spleen. Infected chickens fed the XT-supplemented diet had increased body weight and reduced gut lesion scores compared with infected birds given the non-supplemented diet. The XT-fed group also displayed decreased serum α-toxin levels and reduced intestinal IL-8, lipopolysaccharide-induced TNF-α factor (LITAF), IL-17A and IL-17F mRNA levels, while cytokine/chemokine levels in splenocytes increased in the XT-fed group, compared with the animals fed the control diet. In conclusion, the present study documents the molecular and cellular immune changes following dietary supplementation with extracts of Capsicum and turmeric that may be relevant to protective immunity against avian N

Phase I dose-escalation study of the mTOR inhibitor sirolimus and the HDAC inhibitor vorinostat in patients with advanced malignancy.

Preclinical models suggest that histone deacetylase (HDAC) and mammalian target of rapamycin (mTOR) inhibitors have synergistic anticancer activity. We designed a phase I study to determine the safety, maximum tolerated dose (MTD), recommended phase II dose (RP2D), and dose-limiting toxicities (DLTs) of combined mTOR inhibitor sirolimus (1 mg-5 mg PO daily) and HDAC inhibitor vorinostat (100 mg-400 mg PO daily) in patients with advanced cancer. Seventy patients were enrolled and 46 (66%) were evaluable for DLT assessment since they completed cycle 1 without dose modification unless they had DLT. DLTs comprised grade 4 thrombocytopenia (n = 6) and grade 3 mucositis (n = 1). Sirolimus 4 mg and vorinostat 300 mg was declared RP2D because MTD with sirolimus 5 mg caused significant thrombocytopenia. The grade 3 and 4 drug-related toxic effects (including DLTs) were thrombocytopenia (31%), neutropenia (8%), anemia (7%), fatigue (3%), mucositis (1%), diarrhea (1%), and hyperglycemia (1%). Of the 70 patients, 35 (50%) required dose interruption or modification and 61 were evaluable for response. Partial responses were observed in refractory Hodgkin lymphoma (-78%) and perivascular epithelioid tumor (-54%), and stable disease in hepatocellular carcinoma and fibromyxoid sarcoma. In conclusion, the combination of sirolimus and vorinostat was feasible, with thrombocytopenia as the main DLT. Preliminary anticancer activity was observed in patients with refractory Hodgkin lymphoma, perivascular epithelioid tumor, and hepatocellular carcinoma