The FAD Cofactor of RebC Shifts to an IN Conformation upon Flavin Reduction†,‡

RebC is a putative flavin hydroxylase functioning together with RebP to carry out a key step in the biosynthesis of rebeccamycin. To probe the mechanism of flavin-based chemistry in RebC, we solved the structure of RebC with reduced flavin. Upon flavin reduction, the RebC crystal undergoes a change in its unit cell dimension concurrent with a 5 Å movement of the isoalloxazine ring, positioning the flavin ring adjacent to the substrate-binding pocket. Additionally, a disordered helix becomes ordered upon flavin reduction, closing off one side of the substrate-binding pocket. This structure, along with previously reported structures, increases our understanding of the RebC enzyme mechanism, indicating that either the reduction of the flavin itself or binding of substrate is sufficient to drive major conformational changes in RebC to generate a closed active site. Our finding that flavin reduction seals the active site such that substrate cannot enter suggests that our reduced flavin RebC structure is off-pathway and that substrate binding is likely to precede flavin reduction during catalysis. Along with kinetic data presented here, these structures suggest that the first cycle of catalysis in RebC may resemble that of p-hydroxybenzoate hydroxylase, with substrate binding promoting flavin reduction

Mitogenomics supports an unexpected taxonomic relationship for the extinct diving duck Chendytes lawi and definitively places the extinct Labrador Duck

Chendytes lawi, an extinct flightless diving anseriform from coastal California, was traditionally classified as a sea duck, tribe Mergini, based on similarities in osteological characters. We recover and analyze mitochondrial genomes of C. lawi and five additional Mergini species, including the extinct Labrador Duck, Camptorhyncus labradorius. Despite its diving morphology, C. lawi is reconstructed as an ancient relictual lineage basal to the dabbling ducks (tribe Anatini), revealing an additional example of convergent evolution of characters related to feeding behavior among ducks. The Labrador Duck is sister to Steller’s Eider which may provide insights into the evolution and ecology of this poorly known extinct species. Our results demonstrate that inclusion of full length mitogenomes, from taxonomically distributed ancient and modern sources can improve phylogeny reconstruction of groups previously assessed with shorter single-gene mitochondrial sequences

X-ray Limits on the Progenitor System of the Type Ia Supernova 2017ejb

We present deep X-ray limits on the presence of a pre-explosion counterpart to the low-luminosity Type Ia supernova (SN Ia) 2017ejb. SN 2017ejb was discovered in NGC 4696, a well-studied elliptical galaxy in the Centaurus cluster with 894 ks of Chandra imaging between 14 and 3 years before SN 2017ejb was discovered. Using post-explosion photometry and spectroscopy of SN 2017ejb, we demonstrate that SN 2017ejb is most consistent with low-luminosity SNe Ia such as SN 1986G and SN 1991bg. Analyzing the location of SN 2017ejb in pre-explosion images, we do not detect a pre-explosion X-ray source. We use these data to place upper limits on the presence of any unobscured supersoft X-ray source (SSS). SSS systems are known to consist of white dwarfs accreting from a non-degenerate companion star. We rule out any source similar to known SSS systems with $kT_{\rm eff} > 85$ eV and $L_{\rm bol} > 4\times10^{38}~\text{erg s}^{-1}$ as well as models of stably-accreting Chandrasekhar-mass WDs with accretion rates $\dot{M}>3\times10^{-8}~M_{\odot}~\text{yr}^{-1}$. These findings suggest that low-luminosity SNe Ia similar to SN 2017ejb explode from WDs that are low-mass, have low pre-explosion accretion rates, or accrete very soon before explosion. Based on the limits from SN 2017ejb and other nearby SNe Ia, we infer that $<$47% of SNe Ia explode in stably-accreting Chandrasekhar-mass SSS systems.Comment: 10 pages, 4 figures, submitted to MNRA

Dust in Comet C/2007 N3 (Lulin)

We report optical imaging, optical and near-infrared polarimetry, and Spitzer mid-infrared spectroscopy of comet C/2007 N3 (Lulin). Polarimetric observations were obtained in R (0.676 micron) at phase angles from 0.44 degrees to 21 degrees with simultaneous observations in H (1.65 micron) at 4.0 degrees, exploring the negative branch in polarization. Comet C/2007 N3 (Lulin) shows typical negative polarization in the optical as well as a similar negative branch near-infrared wavelengths. The 10 micron silicate feature is only weakly in emission and according to our thermal models, is consistent with emission from a mixture of silicate and carbon material. We argue that large, low-porosity (akin to Ballistic Particle Cluster Aggregates) rather absorbing aggregate dust particles best explain both the polarimetric and the mid-infrared spectral energy distribution.Comment: 18 pages, 9 figures, 3 table

Forecasting the inundation of postfire debris flows

In the semi-arid regions of the western United States, postfire debris flows are typically runoff generated. The U.S. Geological Survey has been studying the mechanisms of postfire debris-flow initiation for multiple decades to generate operational models for forecasting the timing, location, and magnitude of postfire debris flows. Here we discuss challenges and progress for extending operational capabilities to include modeling postfire debris-flow inundation extent. Analysis of volume and impacted area scaling relationships indicated that postfire debris flows do not conform to assumptions of geometric self-similarity. We documented sensitivity of impacted areas to rainfall intensity using a candidate methodology for generating inundation hazard assessments. Our results emphasize the importance of direct measurements of debris-flow volume, inundated area, and high temporal resolution rainfall intensity

Characteristics of patients making serious inhaler errors with a dry powder inhaler and association with asthma-related events in a primary care setting

Acknowledgements The iHARP database was funded by unrestricted grants from Mundipharma International Ltd and Research in Real-Life Ltd; these analyses were funded by an unrestricted grant from Teva Pharmaceuticals. Mundipharma and Teva played no role in study conduct or analysis and did not modify or approve the manuscript. The authors wish to direct a special appreciation to all the participants of the iHARP group who contributed data to this study and to Mundipharma, sponsors of the iHARP group. In addition, we thank Julie von Ziegenweidt for assistance with data extraction and Anna Gilchrist and Valerie L. Ashton, PhD, for editorial assistance. Elizabeth V. Hillyer, DVM, provided editorial and writing support, funded by Research in Real-Life, Ltd.Peer reviewedPublisher PD

Longitudinal Research on Aging Drivers (LongROAD): study design and methods

Background: As an important indicator of mobility, driving confers a host of social and health benefits to older adults. Despite the importance of safe mobility as the population ages, longitudinal data are lacking about the natural history and determinants of driving safety in older adults. Methods: The Longitudinal Research on Aging Drivers (LongROAD) project is a multisite prospective cohort study designed to generate empirical data for understanding the role of medical, behavioral, environmental and technological factors in driving safety during the process of aging. Results: A total of 2990 active drivers aged 65–79 years at baseline have been recruited through primary care clinics or health care systems in five study sites located in California, Colorado, Maryland, Michigan, and New York. Consented participants were assessed at baseline with standardized research protocols and instruments, including vehicle inspection, functional performance tests, and “brown-bag review” of medications. The primary vehicle of each participant was instrumented with a small data collection device that records detailed driving data whenever the vehicle is operating and detects when a participant is driving. Annual follow-up is being conducted for up to three years with a telephone questionnaire at 12 and 36 months and in-person assessment at 24 months. Medical records are reviewed annually to collect information on clinical diagnoses and healthcare utilization. Driving records, including crashes and violations, are collected annually from state motor vehicle departments. Pilot testing was conducted on 56 volunteers during March–May 2015. Recruitment and enrollment were completed between July 2015 and March 2017. Conclusions: Results of the LongROAD project will generate much-needed evidence for formulating public policy and developing intervention programs to maintain safe mobility while ensuring well-being for older adults

Effect of vessel wettability on the foamability of "ideal" surfactants and "real-world" beer heads

The ability to tailor the foaming properties of a solution by controlling its chemical composition is highly desirable and has been the subject of extensive research driven by a range of applications. However, the control of foams by varying the wettability of the foaming vessel has been less widely reported. This work investigates the effect of the wettability of the side walls of vessels used for the in situ generation of foam by shaking aqueous solutions of three different types of model surfactant systems (non-ionic, anionic and cationic surfactants) along with four different beers (Guinness Original, Banks’s Bitter, Bass No 1 and Harvest Pale). We found that hydrophilic vials increased the foamability only for the three model systems but increased foam stability for all foams except the model cationic system. We then compared stability of beer foams produced by shaking and pouring and demonstrated weak qualitative agreement between both foam methods. We also showed how wettability of the glass controls bubble nucleation for beers and champagne and used this effect to control exactly where bubbles form using simple wettability patterns

A Cool and Inflated Progenitor Candidate for the Type Ib Supernova 2019yvr at 2.6 Years Before Explosion

We present Hubble Space Telescope imaging of a pre-explosion counterpart to SN 2019yvr obtained 2.6 years before its explosion as a type Ib supernova (SN Ib). Aligning to a post-explosion Gemini-S/GSAOI image, we demonstrate that there is a single source consistent with being the SN 2019yvr progenitor system, the second SN Ib progenitor candidate after iPTF13bvn. We also analyzed pre-explosion Spitzer/IRAC imaging, but we do not detect any counterparts at the SN location. SN 2019yvr was highly reddened, and comparing its spectra and photometry to those of other, less extinguished SNe Ib we derive $E(B-V)=0.51\substack{+0.27\\-0.16}$ mag for SN 2019yvr. Correcting photometry of the pre-explosion source for dust reddening, we determine that this source is consistent with a $\log(L/L_{\odot}) = 5.3 \pm 0.2$ and $T_{\mathrm{eff}} = 6800\substack{+400\\-200}$ K star. This relatively cool photospheric temperature implies a radius of 320$\substack{+30\\-50} R_{\odot}$, much larger than expectations for SN Ib progenitor stars with trace amounts of hydrogen but in agreement with previously identified SN IIb progenitor systems. The photometry of the system is also consistent with binary star models that undergo common envelope evolution, leading to a primary star hydrogen envelope mass that is mostly depleted but seemingly in conflict with the SN Ib classification of SN 2019yvr. SN 2019yvr had signatures of strong circumstellar interaction in late-time ($>$150 day) spectra and imaging, and so we consider eruptive mass loss and common envelope evolution scenarios that explain the SN Ib spectroscopic class, pre-explosion counterpart, and dense circumstellar material. We also hypothesize that the apparent inflation could be caused by a quasi-photosphere formed in an extended, low-density envelope or circumstellar matter around the primary star.Comment: 22 pages, 9 figures, submitted to MNRA