Spawning of Threatened Barred Galaxias, Galaxias fuscus (Teleostei: Galaxiidae)

Barred galaxias Galaxias fuscus is an endangered freshwater fish endemic to south-eastern Australia. Little is known of the species’ ecology. We investigated spawning biology of G. fuscus in three headwater streams and found spawning to occur mid-August to late September when photoperiod was 10 h 39 min – 12 h 25 min. Spawning sites were in fresh (range 35.3 – 56.6 EC, mean 44.7 EC), slightly acidic (range 5.7 – 7.1 pH, mean 5.9 pH), moderate to fast flowing (range 0.4 – 2.0 m/s, mean 1.0 m/s), shallow (range 70 – 310 mm, mean 174 mm), well oxygenated (range 10.8 – 12.4 mg/l, mean 11.3mg/l), clear (range 1.2 – 6.3 NTU, mean 3.8 NTU), cool waters (range 8.4 – 10 °C, mean 9.1°C) immediately upstream of pools. Multi-layered clusters of up to 218 eggs were generally adhered close to the stream bed on the downstream side of cobbles greater than 180 mm diameter

Involvement of lactate and pyruvate in the anti-inflammatory effects exerted by voluntary activation of the sympathetic nervous system

We recently demonstrated that the sympathetic nervous system can be voluntarily activated following a training program consisting of cold exposure, breathing exercises, and meditation. This resulted in profound attenuation of the systemic inflammatory response elicited by lipopolysaccharide (LPS) administration. Herein, we assessed whether this training program affects the plasma metabolome and if these changes are linked to the immunomodulatory effects observed. A total of 224 metabolites were identified in plasma obtained from 24 healthy male volunteers at six timepoints, of which 98 were significantly altered following LPS administration. Effects of the training program were most prominent shortly after initiation of the acquired breathing exercises but prior to LPS administration, and point towards increased activation of the Cori cycle. Elevated concentrations of lactate and pyruvate in trained individuals correlated with enhanced levels of anti-inflammatory interleukin (IL)-10. In vitro validation experiments revealed that co-incubation with lactate and pyruvate enhances IL-10 production and attenuates the release of pro-inflammatory IL-1β and IL-6 by LPS-stimulated leukocytes. Our results demonstrate that practicing the breathing exercises acquired during the training program results in increased activity of the Cori cycle. Furthermore, this work uncovers an important role of lactate and pyruvate in the anti-inflammatory phenotype observed in trained subjects.</p

Metabolomics and lipidomics reveal perturbation of sphingolipid metabolism by a novel anti-trypanosomal 3-(oxazolo[4,5-b]pyridine-2-yl)anilide

Introduction: Trypanosoma brucei is the causative agent of human African trypanosomiasis, which is responsible for thousands of deaths every year. Current therapies are limited and there is an urgent need to develop new drugs. The anti-trypanosomal compound, 3-(oxazolo[4,5-b]pyridine-2-yl)anilide (OXPA), was initially identified in a phenotypic screen and subsequently optimized by structure–activity directed medicinal chemistry. It has been shown to be non-toxic and to be active against a number of trypanosomatid parasites. However, nothing is known about its mechanism of action. Objective: Here, we have utilized an untargeted metabolomics approach to investigate the biochemical effects and potential mode of action of this compound in T. brucei. Methods: Total metabolite extracts were analysed by HILIC-chromatography coupled to high resolution mass spectrometry. Results: Significant accumulation of ceramides was observed in OXPA-treated T. brucei. To further understand drug-induced changes in lipid metabolism, a lipidomics method was developed which enables the measurement of hundreds of lipids with high throughput and precision. The application of this LC–MS based approach to cultured bloodstream-form T. brucei putatively identified over 500 lipids in the parasite including glycerophospholipids, sphingolipids and fatty acyls, and confirmed the OXPA-induced accumulation of ceramides. Labelling with BODIPY-ceramide further confirmed the ceramide accumulation following drug treatment. Conclusion: These findings clearly demonstrate perturbation of ceramide metabolism by OXPA and indicate that the sphingolipid pathway is a promising drug target in T. brucei.No Full Tex

First results of archaeological research in the Colorado-Negro interfluvium (Pichi Mahuida department, Río Negro province, Argentina)

En este trabajo se presentan los resultados de las primeras investigaciones arqueológicas realizadas en el interfluvio ubicado entre los cursos medios de los ríos Colorado y Negro (Provincia de Río Negro, Argentina). En esta extensa planicie árida se encuentran lagunas temporarias en las que se concentra el material arqueológico, principalmente en superficie. Con el fin de comprender las estrategias humanas de uso del espacio y la intensidad de las ocupaciones se llevaron a cabo estudios distribucionales y, en menor medida, excavaciones arqueológicas. La información generada fue discutida a la luz de las expectativas arqueológicas derivadas del modelo de uso del espacio propuesto por Borrero y colaboradores (2008). Los resultados obtenidos indican que el registro arqueológico localizado en torno a estas lagunas efímeras es principalmente el efecto de un uso del espacio planificado y redundante. Asimismo, algunos sectores de estas lagunas fueron utilizados más intensa y repetidamente, sugiriendo casos de redundancia específica. Si bien no se cuenta con fechados radiocarbónicos, la presencia de cerámica permite vincular las ocupaciones humanas al menos al Holoceno Tardío. La evidencia discutida revela la importancia de este espacio interfluvial en los circuitos de asentamiento y movilidad de las sociedades cazadoras-recolectoras en torno a dos de los principales ríos norpatagónicos.This paper presents the first results of the archaeological investigations carried out in the interfluvium located between the middle courses of the Colorado and Negro rivers (Río Negro province, Argentina). This is an extensive arid plain with many temporary lagoons in which archaeological material is concentrated, mainly on the surface. Our analysis included distributional studies and, to a lesser extent, archaeological excavations, in order to understand the human strategies of space use and occupational intensity. The generated information is discussed in the light of archaeological expectations derived from the space use model proposed by Borrero and colleagues (2008). The results obtained indicate that the archaeological record located around these ephemeral lagoons is mainly the result of a planned and redundant use of space. Furthermore, some sectors of these lagoons were used more intensively and repeatedly, suggesting cases of specific redundancy. Although radiocarbon dates could not be obtained, the presence of pottery relates human occupations at least to the Late Holocene. The evidence discussed reveals the importance of this interfluvial space in the settlement and mobility strategies of the hunter-gatherer societies that inhabited two of the main northern Patagonian rivers.Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Santos Valero, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Alcaraz, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Borges Vaz, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Stoessel, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Flensborg, Gustavo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Ramos Martínez, Gustavo Antonio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Geología de Costas y del Cuaternario. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto de Geología de Costas y del Cuaternario; ArgentinaFil: Rafuse, Daniel Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; Argentin

A ausculta cardíaca é eficaz para o diagnóstico de sopros em crianças? - revisão de literatura científica / Is heart auscultation effective for the diagnosis of chips in children? - scientific literature review

Objetivo: Este trabalho objetiva compreender a eficácia da ausculta cardíaca quanto ao diagnóstico de sopro em crianças. Método: A partir da seleção de publicações relevantes nas bases de dados Pubmed, Medline e Lilacs, utilizou-se como critérios de seleção artigos relacionados ao tema, de disponibilidade online, publicados entre 2001 e 2017, nas línguas: inglesa, espanhola, portuguesa e francesa. Na seleção dos textos foram utilizados os descritores (DeCs) “auscultação”, “sopros cardíacos”, “criança” e “diagnóstico” na língua portuguesa e na língua inglesa foram “auscultation”, “heart murmurs”, “child” e “diagnoses”. Resultados: Na utilização de tais descritores foram encontrados 510 artigos científicos, onde foram selecionados por meio da leitura de seus resumos, identificando as publicações com abordagem mais semiológica e segundo os critérios já mencionados, 24 artigos. Destes 10 encontrados no Pubmed e 11 na base de dados Lilacs, sendo 17 em inglês, 3 em português e 1 em francês. A literatura utilizada aponta por totalidade dos mesmo que os exames computadorizados e gráficos como ecocardiografia e eletrocardiografia, oferecem um diagnóstico definitivo nas patologias cardíacas pediátricas. É verificado pela mesma totalidade dentre os autores, a importância do exame de auscultação cardíaca em crianças dada a sua alta fidedignidade (98,8%) no diagnóstico de sopro inocente, a auscultação pode poupar recursos, uma vez que todos os autores revisados reconhecem como os exames computadorizados e eletrônicos são dispendiosos e por vezes inacessíveis. Conclusão: A auscultação cardíaca tem grande vantagem no diagnóstico de sopros no que tange o custo/benefício, porém, necessita de um médico capaz e experiente para identificar seus resultados.

Promising Metabolite Profiles in the Plasma and CSF of Early Clinical Parkinson's Disease

Parkinson's disease (PD) shows high heterogeneity with regard to the underlying molecular pathogenesis involving multiple pathways and mechanisms. Diagnosis is still challenging and rests entirely on clinical features. Thus, there is an urgent need for robust diagnostic biofluid markers. Untargeted metabolomics allows establishing low-molecular compound biomarkers in a wide range of complex diseases by the measurement of various molecular classes in biofluids such as blood plasma, serum, and cerebrospinal fluid (CSF). Here, we applied untargeted high-resolution mass spectrometry to determine plasma and CSF metabolite profiles. We semiquantitatively determined small-molecule levels (≤1.5 kDa) in the plasma and CSF from early PD patients (disease duration 0-4 years; n = 80 and 40, respectively), and sex- and age-matched controls (n = 76 and 38, respectively). We performed statistical analyses utilizing partial least square and random forest analysis with a 70/30 training and testing split approach, leading to the identification of 20 promising plasma and 14 CSF metabolites. These metabolites differentiated the test set with an AUC of 0.8 (plasma) and 0.9 (CSF). Characteristics of the metabolites indicate perturbations in the glycerophospholipid, sphingolipid, and amino acid metabolism in PD, which underscores the high power of metabolomic approaches. Further studies will enable to develop a potential metabolite-based biomarker panel specific for PD

A compendium of ecological knowledge for restoration of freshwater fishes in Australia’s Murray–Darling Basin

Many freshwater fishes are imperilled globally, and there is a need for easily accessible, contemporary ecological knowledge to guide management. This compendium contains knowledge collated from over 600 publications and 27 expert workshops to support the restoration of 9 priority native freshwater fish species, representative of the range of life-history strategies and values in south-eastern Australia’s Murray–Darling Basin. To help prioritise future research investment and restoration actions, ecological knowledge and threats were assessed for each species and life stage. There is considerable new knowledge (80% of publications used were from the past 20 years), but this varied among species and life stages, with most known about adults, then egg, juvenile and larval stages (in that order). The biggest knowledge gaps concerned early life stage requirements, survival, recruitment, growth rates, condition and movements. Key threats include reduced longitudinal and lateral connectivity, altered flows, loss of refugia, reductions in both flowing (lotic) and slackwater riverine habitats, degradation of wetland habitats, alien species interactions and loss of aquatic vegetation. Examples and case studies illustrating the application of this knowledge to underpin effective restoration management are provided. This extensive ecological evidence base for multiple species is presented in a tabular format to assist a range of readers

Promising Metabolite Profiles in the Plasma and CSF of Early Clinical Parkinson's Disease

Parkinson's disease (PD) shows high heterogeneity with regard to the underlying molecular pathogenesis involving multiple pathways and mechanisms. Diagnosis is still challenging and rests entirely on clinical features. Thus, there is an urgent need for robust diagnostic biofluid markers. Untargeted metabolomics allows establishing low-molecular compound biomarkers in a wide range of complex diseases by the measurement of various molecular classes in biofluids such as blood plasma, serum, and cerebrospinal fluid (CSF). Here, we applied untargeted high-resolution mass spectrometry to determine plasma and CSF metabolite profiles. We semiquantitatively determined small-molecule levels (≤1.5 kDa) in the plasma and CSF from early PD patients (disease duration 0–4 years; n = 80 and 40, respectively), and sex- and age-matched controls (n = 76 and 38, respectively). We performed statistical analyses utilizing partial least square and random forest analysis with a 70/30 training and testing split approach, leading to the identification of 20 promising plasma and 14 CSF metabolites. These metabolites differentiated the test set with an AUC of 0.8 (plasma) and 0.9 (CSF). Characteristics of the metabolites indicate perturbations in the glycerophospholipid, sphingolipid, and amino acid metabolism in PD, which underscores the high power of metabolomic approaches. Further studies will enable to develop a potential metabolite-based biomarker panel specific for PD

Novel Biomarkers for diagnosis and progression of primary progressive multiple sclerosis (PPMS)

The present invention relates to a method of diagnosing primary progressive multiple sclerosis (PPMS) in a patient suspected of having PPMS. Further, the present invention relates to a method of determining the course of PPMS in a patient having PPMS. Furthermore, the present invention relates to a method of determining the severity of PPMS in a patient suspected of having PPMS. It also relates to the use of at least one metabolite for diagnosing PPMS in a patient suspected of having PPMS, for determining the course of PPMS in a patient having PPMS, or for determining the severity of PPMS in a patient suspected of having PPMS. In addition, it relates to a kit for diagnosing PPMS in a patient suspected of having PPMS, for determining the course of PPMS in a patient having PPMS, or for determining the severity of PPMS in a patient suspected of having PPMS

Spawning of threatened barred galaxias, \u27Galaxias fuscus\u27 (Teleostei: Galaxiidae)

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