Advanced nanostructured medical device combining mesenchymal cells and VEGF nanoparticles for enhanced engineered tissue vascularization.

AIM: Success of functional vascularized tissue repair depends on vascular support system supply and still remains challenging. Our objective was to develop a nanoactive implant enhancing endothelial cell activity, particularly for bone tissue engineering in the regenerative medicine field. MATERIALS & METHODS: We developed a new strategy of tridimensional implant based on cell-dependent sustained release of VEGF nanoparticles. These nanoparticles were homogeneously distributed within nanoreservoirs onto the porous scaffold, with quicker reorganization of endothelial cells. Moreover, the activity of this active smart implant on cells was also modulated by addition of osteoblastic cells. RESULTS & CONCLUSION: This sophisticated active strategy should potentiate efficiency of current therapeutic implants for bone repair, avoiding the need for bone substitutes

Régénération des lésions osseuses maxillo-faciales : épidémiologie, stratégies innovantes au service des patients, qualité et réflexions éthiques

Current treatments of maxillofacial bone defects have now been proven. Only the autogenous graft presents the ideal properties but shows complications: chronic pain, infection... Some bone filling techniques that are currently available do not allow the formation of blood vessels, guaranteeing the sustainability of the regenerated tissue for large lesions. It is then necessary to develop implants in that way, and to find ways to fight effectively the risk of infection. This work presents the results of research conducted on the fabrication of nanofibrous implants mimicking the ECM of bone tissue, with a porosity that is favorable to a vascular formation. These implants can be functionalized with growth factors / cells. Ethical considerations are provided on the development of these advances, but also on their applications to ensure that these developments constitute a real progress in the interest of patients. Moreover, this work shows that it is possible to improve the safety of care in the treatment of maxillofacial bone defects, with the development of equipment in the field of hygiene and the establishment of procedures to assess their effectiveness.Les traitements actuels des lésions osseuses maxillo-faciales ont été éprouvés. La greffe autogène présente les propriétés idéales, mais montre des inconvénients : douleurs chroniques, infection… Certains comblements proposés ne permettent pas une néoformation vasculaire, garante de la viabilité des tissus régénérés pour des lésions importantes. Il faut alors développer des implants avec les caractéristiques recherchées et trouver les moyens de lutter contre le risque infectieux. Ce travail présente les résultats de recherches menées sur la fabrication d’implants nanofibreux mimant la MEC du tissu osseux, dotés d’une porosité favorable à une formation vasculaire et pouvant être fonctionnalisés par des facteurs de croissance / des cellules. Une réflexion éthique est menée sur le développement de ces avancées et sur leurs applications afin de garantir qu’elles constituent un réel progrès pour les patients. Il est aussi montré que l’on peut améliorer la sécurité des soins dans le traitement des lésions osseuses maxillo-faciales en développant des équipements dans le champ de l’hygiène et par la mise en place de procédures visant à évaluer leur efficacité

Regeneration of maxillofacial bone defects : epidemiology, innovative strategies for the patients, quality, and ethical considerations

Les traitements actuels des lésions osseuses maxillo-faciales ont été éprouvés. La greffe autogène présente les propriétés idéales, mais montre des inconvénients : douleurs chroniques, infection… Certains comblements proposés ne permettent pas une néoformation vasculaire, garante de la viabilité des tissus régénérés pour des lésions importantes. Il faut alors développer des implants avec les caractéristiques recherchées et trouver les moyens de lutter contre le risque infectieux. Ce travail présente les résultats de recherches menées sur la fabrication d’implants nanofibreux mimant la MEC du tissu osseux, dotés d’une porosité favorable à une formation vasculaire et pouvant être fonctionnalisés par des facteurs de croissance / des cellules. Une réflexion éthique est menée sur le développement de ces avancées et sur leurs applications afin de garantir qu’elles constituent un réel progrès pour les patients. Il est aussi montré que l’on peut améliorer la sécurité des soins dans le traitement des lésions osseuses maxillo-faciales en développant des équipements dans le champ de l’hygiène et par la mise en place de procédures visant à évaluer leur efficacité.Current treatments of maxillofacial bone defects have now been proven. Only the autogenous graft presents the ideal properties but shows complications: chronic pain, infection... Some bone filling techniques that are currently available do not allow the formation of blood vessels, guaranteeing the sustainability of the regenerated tissue for large lesions. It is then necessary to develop implants in that way, and to find ways to fight effectively the risk of infection. This work presents the results of research conducted on the fabrication of nanofibrous implants mimicking the ECM of bone tissue, with a porosity that is favorable to a vascular formation. These implants can be functionalized with growth factors / cells. Ethical considerations are provided on the development of these advances, but also on their applications to ensure that these developments constitute a real progress in the interest of patients. Moreover, this work shows that it is possible to improve the safety of care in the treatment of maxillofacial bone defects, with the development of equipment in the field of hygiene and the establishment of procedures to assess their effectiveness

L' évolution de l'image du chirurgien-dentiste pour le patient, en France, depuis la création de l'ordre de Saint Côme (1268) jusqu'au XIXème siècle

STRASBOURG-Medecine (674822101) / SudocSudocFranceF

Analyse éthique des leviers de l'esthétique dentaire pour le patient et pour le praticien (entre normativité et coercition)

STRASBOURG-Medecine (674822101) / SudocSudocFranceF

Etude comparative de différents tuyaux d'aspiration en pratique de médecine et chirurgie bucco-dentaires dans le champ de l'hygiène

STRASBOURG-Medecine (674822101) / SudocSudocFranceF

An Evaluation of Two Systems for the Management of the Microbiological Quality of Water in Dental Unit Waterlines: Hygowater® and IGN Calbénium®

International audienceWater in dental unit waterlines (DUWL) represents a risk for vulnerable patients if its microbiological quality is not controlled. The aim of this prospective study was to evaluate two systems for its management under real conditions: Hygowater® and IGN Calbenium®. Samples of the output water of DUWL were obtained for 5 previously contaminated units connected to Hygowater®, and 5 non-contaminated units connected to IGN Calbenium®, which was already effective for more than 1 year, as a control group. Samples were regularly collected up to 6 months after the implementation of Hygowater®, and were then cultured and analyzed. With IGN Calbenium®, except for a technical problem and a sample result in one unit at 6 months (Heterotrophic Plate Count (HPC) at 37 °C of 66 colony forming units (cfu)/mL), the results showed an absence of contamination. Hygowater® took a couple of weeks to be effective on initially contaminated DUWL (over 200 cfu/mL for all the units), then showed its efficacy for 2 months (HPC at 37 °C with a mean of 40.2 ufc/mL, and HPC at 22 °C with a mean of 0.2 ufc/mL). At 6 months, results were satisfactory for HPC at 22 °C (mean of 12 ufc/mL), but HPC at 37 °C gave non-satisfactory results for 4 of the 5 units (mean of 92.2 ufc/mL). Both systems have an effect on the microbiological quality of DUWL. IGN Calbenium® appears to be more reliable on a long-term basis

Intérêt prophylactique et thérapeutique des chewing-gums sans sucre en orthodontie. Une étude menée auprès de professionnels de santé et de patients [Preventive and therapeutic advantages of sugar-free chewing gums in orthodontics. A study conducted on practitioners and patients]

OBJECTIVE: The objective of this study is to assess the level of knowledge in a cohort of oral health professionals and patients about preventive and therapeutic actions of sugar-free chewing gums. MATERIALS AND METHODS: A forward-looking monocentric study of perception regarding the level of information about the effects of sugar-free chewing gums consumption was conducted on 135 young patients, from 11 to 17 years old, carriers of fixed orthodontic appliances and treated in the Department of Orthodontics in the Oral Medicine and Surgery Center of the University Hospitals of Strasbourg. Besides, 34 practitioners in the Department of Orthodontics and Pediatric Dentistry were also included. Data were collected between May 2016 and July 2016. A specific questionnaire, using the adapted terminology and dealing with the same items was developed for each studied population. RESULTS: The majority of the individuals of both studied populations believe that the consumption of sugar-free chewing gum leads to a greater risk of orthodontic device unsticking or fracture and that it is not associated to a decrease of the orthodontic pain. DISCUSSION: Our results confirm the fact that the knowledge, mainly acquired in an empirical way, is against the data of the current literature. The evaluation of the level of knowledge demonstrated that there is a real lack of information about the preventive interests of the consumption of sugar-free chewing gums during orthodontic treatment. CONCLUSIONS: This study highlights the need for information campaigns and oral prevention in general population, as well as in healthcare professionals, concerning the preventive and analgesic interests of sugar-free chewing gums during orthodontic treatments

The “new normal” of hygiene measures at the end of the COVID-19 epidemic: a survey among French dentists

Abstract Objectives The COVID-19 epidemic upset the standards in terms of hygiene and protection in the dental office, bringing additional precautions for dentists. The objective of our study was to draw the “new normal” of hygiene measures at the end of the COVID-19 epidemic. Materials and methods A self-administered questionnaire about transitional recommendations for oral care in the context of the COVID-19 epidemic was published online in private groups dedicated to French dentists. Results The 246 respondents understood the reasons behind those recommendations, since 10 out of 11 measures reached a mean score greater than 2.5 on a 0 (not at all) to 4 (absolutely) scale when it came to determining whether the measure made the practitioner feel safe and ensured patient safety. Besides, more of the respondents intended to maintain the measures than they were to apply them before the epidemic. Conclusions The COVID-19 epidemic reshaped the relationship to hygiene and protection measures in the context of dental practices. The “new normal” of hygiene measures at the end of the COVID-19 epidemic will probably involve more protective measures than before. Clinical relevance These results constitute interesting avenues for public health deliberation, which would make it possible to best adapt future health recommendations in order to define the “new normal” of hygiene measures in dental practices at the end of the COVID-19 epidemic. Therefore, it could have an impact on all practitioners in their clinical activities