4,852 research outputs found
Characterization of cellulase production by carbon sources in two Bacillus species
The induction of cellulase production in two Bacillus spp. was studied by means of measuring cellulase activities under the condition of different carbon sources. The results indicate that cellulase could not be induced by cellulose material as a sole carbon source. Instead, they could be induced by monosaccharide or disaccharide with reducing group. Moreover, the expression of cellulase components was synergistic. When cell wall/envelope enzyme and endoenzyme from two Bacillus spp. acted on these inducers, analysis of reaction products by high performance liquid chromatography (HPLC) revealed that cell wall/envelope enzyme and endoenzyme from two Bacillus spp. were inactive on these inducers. It also indicated that these inducers entered cells directly and served function of induction.Keywords: Bacillus, cellulase, induction, carbon source
Interplay of tRNA-Derived Fragments and T Cell Activation in Breast Cancer Patient Survival
Effector CD8+ T cell activation and its cytotoxic function are positively correlated with improved survival in breast cancer. tRNA-derived fragments (tRFs) have recently been found to be involved in gene regulation in cancer progression. However, it is unclear how interactions between expression of tRFs and T cell activation affect breast cancer patient survival. We used Kaplan–Meier survival and multivariate Cox regression models to evaluate the effect of interactions between expression of tRFs and T cell activation on survival in 1081 breast cancer patients. Spearman correlation analysis and weighted gene co-expression network analysis were conducted to identify genes and pathways that were associated with tRFs. tRFdb-5024a, 5P_tRNA-Leu-CAA-4-1, and ts-49 were positively associated with overall survival, while ts-34 and ts-58 were negatively associated with overall survival. Significant interactions were detected between T cell activation and ts-34 and ts-49. In the T cell exhaustion group, patients with a low level of ts-34 or a high level of ts-49 showed improved survival. In contrast, there was no significant difference in the activation group. Breast cancer related pathways were identified for the five tRFs. In conclusion, the identified five tRFs associated with overall survival may serve as therapeutic targets and improve immunotherapy in breast cancer
Single charge control of localized excitons in heterostructures with ferroelectric thin films and two-dimensional transition metal dichalcogenides
Single charge control of localized excitons (LXs) in two-dimensional
transition metal dichalcogenides (TMDCs) is crucial for potential applications
in quantum information processing and storage. However, traditional
electrostatic doping method with applying metallic gates onto TMDCs may cause
the inhomogeneous charge distribution, optical quench, and energy loss. Here,
by locally controlling the ferroelectric polarization of the ferroelectric thin
film BiFeO3 (BFO) with a scanning probe, we can deterministically manipulate
the doping type of monolayer WSe2 to achieve the p-type and n-type doping. This
nonvolatile approach can maintain the doping type and hold the localized
excitonic charges for a long time without applied voltage. Our work
demonstrated that ferroelectric polarization of BFO can control the charges of
LXs effectively. Neutral and charged LXs have been observed in different
ferroelectric polarization regions, confirmed by magnetic optical measurement.
Highly circular polarization degree about 90 % of the photon emission from
these quantum emitters have been achieved in high magnetic fields. Controlling
single charge of LXs in a non-volatile way shows a great potential for
deterministic photon emission with desired charge states for photonic long-term
memory.Comment: 13 pages, 5 figure
Asymmetric Chiral Coupling in a Topological Resonator
Chiral light-matter interactions supported by topological edge modes at the
interface of valley photonic crystals provide a robust method to implement the
unidirectional spin transfer. The valley topological photonic crystals possess
a pair of counterpropagating edge modes. The edge modes are robust against the
sharp bend of and , which can form a resonator with
whispering gallery modes. Here, we demonstrate the asymmetric emission of
chiral coupling from single quantum dots in a topological resonator by tuning
the coupling between a quantum emitter and a resonator mode. Under a magnetic
field in Faraday configuration, the exciton state from a single quantum dot
splits into two exciton spin states with opposite circularly polarized
emissions due to Zeeman effect. Two branches of the quantum dot emissions
couple to a resonator mode in different degrees, resulting in an asymmetric
chiral emission. Without the demanding of site-control of quantum emitters for
chiral quantum optics, an extra degree of freedom to tune the chiral contrast
with a topological resonator could be useful for the development of on-chip
integrated photonic circuits.Comment: 13 pages, 4 figure
Controllable Spin-Resolved Photon Emission Enhanced by Slow-Light Mode in Photonic Crystal Waveguides on Chip
We report the slow-light enhanced spin-resolved in-plane emission from a
single quantum dot (QD) in a photonic crystal waveguide (PCW). The slow light
dispersions in PCWs are designed to match the emission wavelengths of single
QDs. The resonance between two spin states emitted from a single QD and a slow
light mode of a waveguide is investigated under a magnetic field with Faraday
configuration. Two spin states of a single QD experience different degrees of
enhancement as their emission wavelengths are shifted by combining diamagnetic
and Zeeman effects with an optical excitation power control. A circular
polarization degree up to 0.81 is achieved by changing the off-resonant
excitation power. Strongly polarized photon emission enhanced by a slow light
mode shows great potential to attain controllable spin-resolved photon sources
for integrated optical quantum networks on chip.Comment: 7 pages,5 figure
Preparation of a Porous Composite Film for the Fabrication of a Hydrogen Peroxide Sensor
A series of dopant-type polyaniline-polyacrylic acid composite (PAn-PAA) films with porous structures were prepared and developed for an enzyme-free hydrogen peroxide (H2O2) sensor. The composite films were highly electroactive in a neutral environment as compared to polyaniline (PAn). In addition, the carboxyl group of the PAA was found to react with H2O2 to form peroxy acid groups, and the peroxy acid could further oxidize the imine structure of PAn to form N-oxides. The N-oxides reverted to their original form via electrochemical reduction and increased the reduction current. Based on this result, PAn-PAA was used to modify a gold electrode (PAn-PAA/Au) as a working electrode for the non-enzymatic detection of H2O2. The characteristics of the proposed sensors could be tuned by the PAA/PAn molar ratio. Blending PAA with PAn enhanced the surface area, electrocatalytic activity, and conductivity of these sensors. Under optimal conditions, the linear concentration range of the H2O2 sensor was 0.04 to 12 mM with a sensitivity of 417.5 μA/mM-cm2. This enzyme-free H2O2 sensor also exhibited a rapid response time, excellent stability, and high selectivity
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