Clover:cereal bi-cropping for organic farms (OF0173)

This is the final report of Defra project OF0173. The attached main report starts with a more detailed summary, from which this text is extracted. IGER, IACR and others developed a system for growing cereals, especially for whole crop silage, that enables either greatly reduced or zero levels of N fertilizer and agrochemicals to be used. The system is simple and straightforward and relies on direct drilling of the cereal into an established understorey of white clover. The white clover understorey becomes permanent and perennial and successive cereal crops are drilled into it, harvested and re-drilled each year. The clover provides N for the cereal crop and the gross changes in crop architecture that occur (a) encourage large populations of predatory invertebrates that devour pest species and hence obviate the need for insecticide use (b) frustrate the spread of fungal foliar diseases and remove the need to use fungicides and (c) suppress most weeds. Some of the advantages of the clover:cereal bi-cropping system that are especially relevant to organic farms are a) simultaneous cropping and fertility building rather than separate seasons for each b) effective nutrient cycling c) weeds replaced by clover; limitation of crop area requiring intensive weed control d) confusion of insect pests and habitat for beneficial insects, spiders etc. e) improved field access relative to bare soil The work investigated the agronomic viability and sustainability of bi-cropping for both silage and grain production in organic farming systems as specified under UKROFS. Experiments were carried out on three UKROF approved sites with contrasting soil types and environmental conditions. Randomised block, small-plot experiments evaluated the most satisfactory ways of modifying the IGER/IACR system for organic farming. The main point to emerge is that although the bi-crop system is well proven and developed for use in a non-organic system, grass weeds remain a problem in translating the system to organic farming as herbicides can not be used to control them. There are a number of strategies that emerged during the course of the present work that indicated ways in which the grass weed problem may be overcome in future. Firstly, oats seem to suppress grass weeds and growing this crop instead of wheat appeared to show considerable promise. Secondly strip drilling alternate 20 cm strips of cereal with 30 cm strips of clover allows separate management of the two crop components, facilitating weed control. The work confirmed that spring-sown cereals are not an option for bi-cropping as they are easily outcompeted by the clover. An important positive feature of all of the trials was the consistent absence or low levels of diseases and pests despite high levels of airborne pathogen inoculum in the trial area. It is difficult to gauge the relative contributions of the organic system as a whole and the bi-crop system in particular to this feature. There are certainly important theoretical reasons as to why the bi-crop system should help in this direction, including the restriction on spread of splash-borne diseases because of the presence of clover around the cereal plants, green background confusion of insect pests and the probable lack of surplus soluble nitrogen in the cereal plants. The more positive results in the absence of grass weeds were limited to a single year for cereals (and two seasons for vegetables in another related trial). However, in both cases, there were clear indications that, with a relatively small amount of fine tuning of the system, it should be possible to obtain highly acceptable results from both types of inter-cropping and, indeed, from rotational integration of the two. Further progress would certainly be worthwhile for the organic producer because it seems clear that a modest further adjustment of the competitive balance between crop and clover will lead to a clearer expression of all of the potential advantages of such systems, outlined above. Despite the difficulties encountered, progress was made and the list of advantages and potential advantages of bi-cropping for organic agriculture is so large and significant that further work should be done to capitalise on that completed to date

Children’s Views on Playtime in Schools: A Systematic Literature Review

Background and aim(s)Playtime in schools has been shown to have wide-ranging benefits to children and young people. However, opportunities for playtime in schools have declined over time. It is important children’s voices are heard on matters that are important to them, yet playtime is often driven by adult policy. The aim of this systematic literature review (SLR) was to explore children’s views on playtime to further our understanding of what is important to them, and what affects their access to playtime.MethodologyThis SLR employed thematic synthesis to aggregate the views of children on playtime. This review includes 8 papers which met inclusion criteria, and this review drew out children’s views on playtime from each study.FindingsChildren’s views on playtime included both aspects that they enjoyed as well as the barriers that they perceived to enjoying and accessing their playtime. Aspects that they enjoyed included: being outdoors, social interaction and friendships, physical activity, and freedom and autonomy. Aspects that they perceived to be barriers to enjoying and accessing playtime included: social conflict, risk, weather, loss of playtime, and lack of resource and support.LimitationsMost of papers included were from either the UK or USA, with one being from Greece. This is likely to impact the representation of these findings to other countries. The studies did not include the views of older children (14+) or the views of groups that are more likely to face barriers to their play (e.g. children with special educational needs).ConclusionsFrom the studies reviewed, children identified many positive aspects of, as well as barriers to, their playtime. Implications for policy and practice include considerations about length of opportunities for playtime, as well as resources and staffing allocated to managing playtime. Further implications include challenging practice that restricts playtime. This review also highlighted a dearth in research exploring children’s views on playtime as a general concept. Directions for future research are discussed further

A Correlation Study between Religiosity and Empathy toward Victims of Crime

Abstract This present study was conducted to examine the relationship between religious involvement and empathy towards victims of crime. There was a total sample size of 84 individuals that participated in an online survey. Religiosity was measured using the Belief into Action scale. Empathy towards victims of crime was observed using the Victim Impact Scale. A Pearson correlation showed no significant relationship between religious involvement and empathy towards victims. There was, however, a negative significant correlation between Accountability score and percent of income given to religious causes (r=-.297, p=.011), victim blaming and how much time is spent in religious volunteering (r=-.261, p=.025), knowledge of victim-related facts and how often one attends religious services (r=-.263, p=.029), percent of income given to religious causes (r=-.301, p=.012), and time spent in religious volunteering (r=-.312, p=.01)

The Relationship between Adverse Childhood Experiences (ACEs) and Intrinsic Religiosity in Southern Appalachia

The present exploratory study examined adverse childhood experiences and religiosity in a sample of individuals from Southern Appalachia. Self-reports of childhood adversity and intrinsic religion were obtained from 167 individuals. Results showed that fifty-five percent had not experienced a childhood adversity while forty-three percent had experienced one to five episodes. Although a little under half the sample had one or more adverse experiences, there was no significant relationship between ACEs and intrinsic religion (r= -.037, p= .631). Sex and intrinsic religion were significantly related in that men endorsed the use of intrinsic religiosity more than women (t(165)= -2.28, p= .005)

Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity

During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase briefly extends RNA primers synthesized by the primase DnaG prior to handing-off DNA synthesis to PolC. Here, we show in vivo that (i) the polymerase activity of DnaE is essential for both the initiation and elongation stages of DNA replication, (ii) its error rate varies inversely with PolC concentration, and (iii) its misincorporations are corrected by the mismatch repair system post-replication. We also found that the error rates in cells encoding mutator forms of both PolC and DnaE are significantly higher (up to 15-fold) than in PolC mutants. In vitro, we showed that (i) the polymerase activity of DnaE is considerably stimulated by DnaN, SSB and PolC, (ii) its error-prone activity is strongly inhibited by DnaN, and (iii) its errors are proofread by the 30 . 50 exonuclease activity of PolC in a stable template-DnaE –PolC complex. Collectively our data show that protein –protein interactions within the replisome modulate the activity and fidelity of DnaE, and confirm the prominent role of DnaE during B. subtilis replication

Skin dendritic cells in melanoma are key for successful checkpoint blockade therapy.

BACKGROUND: Immunotherapy with checkpoint inhibitors has shown impressive results in patients with melanoma, but still many do not benefit from this line of treatment. A lack of tumor-infiltrating T cells is a common reason for therapy failure but also a loss of intratumoral dendritic cells (DCs) has been described. METHODS: We used the transgenic tg(Grm1)EPv melanoma mouse strain that develops spontaneous, slow-growing tumors to perform immunological analysis during tumor progression. With flow cytometry, the frequencies of DCs and T cells at different tumor stages and the expression of the inhibitory molecules programmed cell death protein-1 (PD-1) and T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) on T cells were analyzed. This was complemented with RNA-sequencing (RNA-seq) and real-time quantitative PCR (RT-qPCR) analysis to investigate the immune status of the tumors. To boost DC numbers and function, we administered Fms-related tyrosine 3 ligand (Flt3L) plus an adjuvant mix of polyI:C and anti-CD40. To enhance T cell function, we tested several checkpoint blockade antibodies. Immunological alterations were characterized in tumor and tumor-draining lymph nodes (LNs) by flow cytometry, CyTOF, microarray and RT-qPCR to understand how immune cells can control tumor growth. The specific role of migratory skin DCs was investigated by coculture of sorted DC subsets with melanoma-specific CD8+ T cells. RESULTS: Our study revealed that tumor progression is characterized by upregulation of checkpoint molecules and a gradual loss of the dermal conventional DC (cDC) 2 subset. Monotherapy with checkpoint blockade could not restore antitumor immunity, whereas boosting DC numbers and activation increased tumor immunogenicity. This was reflected by higher numbers of activated cDC1 and cDC2 as well as CD4+ and CD8+ T cells in treated tumors. At the same time, the DC boost approach reinforced migratory dermal DC subsets to prime gp100-specific CD8+ T cells in tumor-draining LNs that expressed PD-1/TIM-3 and produced interferon γ (IFNγ)/tumor necrosis factor α (TNFα). As a consequence, the combination of the DC boost with antibodies against PD-1 and TIM-3 released the brake from T cells, leading to improved function within the tumors and delayed tumor growth. CONCLUSIONS: Our results set forth the importance of skin DC in cancer immunotherapy, and demonstrates that restoring DC function is key to enhancing tumor immunogenicity and subsequently responsiveness to checkpoint blockade therapy

Regional Endothermy in a Coral Reef Fish?

Although a few pelagic species exhibit regional endothermy, most fish are regarded as ectotherms. However, we document significant regional endothermy in a benthic reef fish. Individual steephead parrotfish, Chlorurus microrhinos (Labridae, formerly Scaridae) were tagged and their internal temperatures were monitored for a 24 h period using active acoustic telemetry. At night, on the reef, C. microrhinos were found to maintain a consistent average peritoneal cavity temperature 0.16±0.005°C (SE) warmer than ambient. Diurnal internal temperatures were highly variable for individuals monitored on the reef, while in tank-based trials, peritoneal cavity temperatures tracked environmental temperatures. The mechanisms responsible for a departure of the peritoneal cavity temperature from environmental temperature occurred in C. microrhinos are not yet understood. However, the diet and behavior of the species suggests that heat in the peritoneal cavity may result primarily from endogenous thermogenesis coupled with physiological heat retention mechanisms. The presence of limited endothermy in C. microrhinos indicates that a degree of uncertainty may exist in the manner that reef fish respond to their thermal environment. At the very least, they do not always appear to respond to environmental temperatures as neutral thermal vessels and do display limited, but significant, visceral warming